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The map label for this gene is rpfG [H]

Identifier: 197117275

GI number: 197117275

Start: 1037724

End: 1038698

Strand: Reverse

Name: rpfG [H]

Synonym: Gbem_0885

Alternate gene names: 197117275

Gene position: 1038698-1037724 (Counterclockwise)

Preceding gene: 197117279

Following gene: 197117272

Centisome position: 22.51

GC content: 60.21

Gene sequence:

>975_bases
ATGCCCAACAAACAAGAACGCGAAACCATCCTTTTCGTCGATGACGAGAGAAGCTACCTCGCCTACACCAGAGGGGTCTT
CGAGAACAGGGGGCTCAACGTACTGACTGCTGCGTCCGCACTGGAGGCGCTGAAGATCGTCGAGGAGCAGAATGTCGCCG
TAGTCATTTCGGACAACGAGATGCCGGGGATGCGTGGAATAGAACTGCTGTCAAAGATCAAGGAGGTCTCGCCGCACACG
GTGAAGATCATGATGACCGGAAGCGCCGACCTCTCCACCACACTGGCCGCCATCAACAGCGGCGAAGTCTTTCGTTTCGT
GCTCAAGCCGTGGAAGAAGGCGGACATGCTGCGTGCGGTGAAAGACGGCATCCGCAGGTACCGCGTGCTGCAGTCGCTCA
AGCGCGAGGACGAGTTCATCCTGTTCTCGCTGGCGCAGACCATCGAGCTGAAGGATGCCTCCACCAAGGGGCACTGCGAT
CGCGTGGCCACCCTCGCCATTAAAATCGCGGAGCGGCTGGGGGTGTCCGAGGAGATAAAGCAGGAGATCAGGTACGGCAG
TTGGTTGCACGACTGCGGCAAGATCGGGGTGCCTGAGGCGATCCTGAACGCGGAGCGCGGCTTGACCGACGAGGAGTTTC
AGGTCGTGATGAAGCACCCGGAATGGGGATGCGAGGTGGTGCGCAAGGCGAACCTATCGAAGACGGTGCAGAACATCGTC
CTTTACCACCACGAACGCTACGACGGCAAGGGGTACCCACAGAAGCTGGACGGAGACCGGATACCGATCGAGGCGCAGAT
AGTGGCGACCGCCGACATCTGCGACGCCCTGACCATGGACCGCCCCTACCGCAAGGGATTCACCCGTGACGAGACGATCG
GGACCATGAACGAGATGAAGGGAAACCACCTGGCGCCGCGCCTGGTGGAGGTCCTTCTGGAGGTTCTGGCCGAAGAGGCC
GAAGTGCAGGGATAA

Upstream 100 bases:

>100_bases
AGATCGCCAGCCTTAATTTTTTTCCCTTTTCTCATGGGAACTTCTGCCGATAAGTACAAGGCACACTGCTTAATGAACTG
TGCCTGCCGGAGATAAACCA

Downstream 100 bases:

>100_bases
AAAAAGGGAGCGCATCGCGCCCCCCTTCGTGTGCCTCTCATGCTGCCCGCCAGGTTACTTCTTCCGGTATTGATCCAAGG
CCTTTCGCACCCGCCCCTCG

Product: response receiver-modulated cyclic diguanylate phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MPNKQERETILFVDDERSYLAYTRGVFENRGLNVLTAASALEALKIVEEQNVAVVISDNEMPGMRGIELLSKIKEVSPHT
VKIMMTGSADLSTTLAAINSGEVFRFVLKPWKKADMLRAVKDGIRRYRVLQSLKREDEFILFSLAQTIELKDASTKGHCD
RVATLAIKIAERLGVSEEIKQEIRYGSWLHDCGKIGVPEAILNAERGLTDEEFQVVMKHPEWGCEVVRKANLSKTVQNIV
LYHHERYDGKGYPQKLDGDRIPIEAQIVATADICDALTMDRPYRKGFTRDETIGTMNEMKGNHLAPRLVEVLLEVLAEEA
EVQG

Sequences:

>Translated_324_residues
MPNKQERETILFVDDERSYLAYTRGVFENRGLNVLTAASALEALKIVEEQNVAVVISDNEMPGMRGIELLSKIKEVSPHT
VKIMMTGSADLSTTLAAINSGEVFRFVLKPWKKADMLRAVKDGIRRYRVLQSLKREDEFILFSLAQTIELKDASTKGHCD
RVATLAIKIAERLGVSEEIKQEIRYGSWLHDCGKIGVPEAILNAERGLTDEEFQVVMKHPEWGCEVVRKANLSKTVQNIV
LYHHERYDGKGYPQKLDGDRIPIEAQIVATADICDALTMDRPYRKGFTRDETIGTMNEMKGNHLAPRLVEVLLEVLAEEA
EVQG
>Mature_323_residues
PNKQERETILFVDDERSYLAYTRGVFENRGLNVLTAASALEALKIVEEQNVAVVISDNEMPGMRGIELLSKIKEVSPHTV
KIMMTGSADLSTTLAAINSGEVFRFVLKPWKKADMLRAVKDGIRRYRVLQSLKREDEFILFSLAQTIELKDASTKGHCDR
VATLAIKIAERLGVSEEIKQEIRYGSWLHDCGKIGVPEAILNAERGLTDEEFQVVMKHPEWGCEVVRKANLSKTVQNIVL
YHHERYDGKGYPQKLDGDRIPIEAQIVATADICDALTMDRPYRKGFTRDETIGTMNEMKGNHLAPRLVEVLLEVLAEEAE
VQG

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=115, Percent_Identity=32.1739130434783, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 36498; Mature: 36367

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNKQERETILFVDDERSYLAYTRGVFENRGLNVLTAASALEALKIVEEQNVAVVISDNE
CCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCEEEEEECCC
MPGMRGIELLSKIKEVSPHTVKIMMTGSADLSTTLAAINSGEVFRFVLKPWKKADMLRAV
CCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCCHHHHHHHCCHHHHHHHHHH
KDGIRRYRVLQSLKREDEFILFSLAQTIELKDASTKGHCDRVATLAIKIAERLGVSEEIK
HHHHHHHHHHHHHHCCCCEEEEEHHHHHEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHH
QEIRYGSWLHDCGKIGVPEAILNAERGLTDEEFQVVMKHPEWGCEVVRKANLSKTVQNIV
HHHHHCHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LYHHERYDGKGYPQKLDGDRIPIEAQIVATADICDALTMDRPYRKGFTRDETIGTMNEMK
HHHHHCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHCCCHHHCCCCCCHHHCCHHHCC
GNHLAPRLVEVLLEVLAEEAEVQG
CCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PNKQERETILFVDDERSYLAYTRGVFENRGLNVLTAASALEALKIVEEQNVAVVISDNE
CCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCEEEEEECCC
MPGMRGIELLSKIKEVSPHTVKIMMTGSADLSTTLAAINSGEVFRFVLKPWKKADMLRAV
CCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCCHHHHHHHCCHHHHHHHHHH
KDGIRRYRVLQSLKREDEFILFSLAQTIELKDASTKGHCDRVATLAIKIAERLGVSEEIK
HHHHHHHHHHHHHHCCCCEEEEEHHHHHEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHH
QEIRYGSWLHDCGKIGVPEAILNAERGLTDEEFQVVMKHPEWGCEVVRKANLSKTVQNIV
HHHHHCHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LYHHERYDGKGYPQKLDGDRIPIEAQIVATADICDALTMDRPYRKGFTRDETIGTMNEMK
HHHHHCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHCCCHHHCCCCCCHHHCCHHHCC
GNHLAPRLVEVLLEVLAEEAEVQG
CCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]