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The map label for this gene is nirB [C]

Identifier: 197117011

GI number: 197117011

Start: 735554

End: 736258

Strand: Direct

Name: nirB [C]

Synonym: Gbem_0614

Alternate gene names: 197117011

Gene position: 735554-736258 (Clockwise)

Preceding gene: 197117010

Following gene: 197117012

Centisome position: 15.94

GC content: 62.41

Gene sequence:

>705_bases
ATGACTGAAGCTATGACCAAGAAAGATATCCCCACTAAAGGCGCGATCCTCCAGCGCGACCGCGAAACCTACGCCATCGC
GCCGCACATTCCGGGCGGCTTCACCGACACGGCGACGCTCAGGAAGATCTGCGACGTGGCGGACAAGTACAACCTGGCTC
TCAAAATCACCTCGGCGCAGCGTATCGCCATGATCGGCGCCAAGGAAGAAGAGCTGGACGCCATCTGGAGCGACTTGGCG
CAGAACCCCGGCGCGGCCATCGGTCTTTGCGTCAGGAGCGTCAAGGTCTGCCCGGGCACCCAGCACTGCAAGAGGGCGGT
ACTCGATTCCGCGTCGCTCGGCCTCAAGCTGGACCGGATCTACCACGCCATGGAACTTCCCAACAAGATGAAGATGGGGG
TCTCCGGCTGCCTCCTTTCCTGCTCGGAGTCGGGCGTGAAGGACATCGGCATCGTCGGGCACAACAAGGGCTGGCGCATC
TTCGTAGGCGGCAACGCCGGCCCCAGGCCGCGCCTGGGCGAAATGCTGGTGGACAACGTGCAGAGCGAGGAAGAGGTGCT
GGAGATCGTCGCCAAGGTGGTCGATTTTTACAAGAACGATCCGCAGCAGGGGCGCATCGGCAAGCTGGTAGAGGAGATGG
GGATAGAGGCTTTCCGTGCCAAGGTCCTGGGAACCGCTGCCGCAGCGGCAGGGAGCGCCAAGTAA

Upstream 100 bases:

>100_bases
GGATTTTCCTCTGTGTACCTCTGTGTCCTCTGTGGTGAGTGCCTTTAAAGTTAGCCGTATTTGACATGCATCAATTTATT
ACCGGACAAAGGAGCAGATG

Downstream 100 bases:

>100_bases
TATGGACGCAAGTACGCAGGATCAGGCGCTCTCCGCGGGAGGTTGCCGTGGCGCTTATTGAGGACGCCGCCTTCACCCGG
ATCAAGAACCGGGTGGGGAA

Product: sulfite reductase, assimilatory

Products: 3 NAD(P)+; NH4OH; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MTEAMTKKDIPTKGAILQRDRETYAIAPHIPGGFTDTATLRKICDVADKYNLALKITSAQRIAMIGAKEEELDAIWSDLA
QNPGAAIGLCVRSVKVCPGTQHCKRAVLDSASLGLKLDRIYHAMELPNKMKMGVSGCLLSCSESGVKDIGIVGHNKGWRI
FVGGNAGPRPRLGEMLVDNVQSEEEVLEIVAKVVDFYKNDPQQGRIGKLVEEMGIEAFRAKVLGTAAAAAGSAK

Sequences:

>Translated_234_residues
MTEAMTKKDIPTKGAILQRDRETYAIAPHIPGGFTDTATLRKICDVADKYNLALKITSAQRIAMIGAKEEELDAIWSDLA
QNPGAAIGLCVRSVKVCPGTQHCKRAVLDSASLGLKLDRIYHAMELPNKMKMGVSGCLLSCSESGVKDIGIVGHNKGWRI
FVGGNAGPRPRLGEMLVDNVQSEEEVLEIVAKVVDFYKNDPQQGRIGKLVEEMGIEAFRAKVLGTAAAAAGSAK
>Mature_233_residues
TEAMTKKDIPTKGAILQRDRETYAIAPHIPGGFTDTATLRKICDVADKYNLALKITSAQRIAMIGAKEEELDAIWSDLAQ
NPGAAIGLCVRSVKVCPGTQHCKRAVLDSASLGLKLDRIYHAMELPNKMKMGVSGCLLSCSESGVKDIGIVGHNKGWRIF
VGGNAGPRPRLGEMLVDNVQSEEEVLEIVAKVVDFYKNDPQQGRIGKLVEEMGIEAFRAKVLGTAAAAAGSAK

Specific function: This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789765, Length=215, Percent_Identity=34.4186046511628, Blast_Score=132, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR017220 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: 1.7.1.4 [C]

Molecular weight: Translated: 25120; Mature: 24989

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAMTKKDIPTKGAILQRDRETYAIAPHIPGGFTDTATLRKICDVADKYNLALKITSAQ
CCCCCCCCCCCCCCHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCC
RIAMIGAKEEELDAIWSDLAQNPGAAIGLCVRSVKVCPGTQHCKRAVLDSASLGLKLDRI
EEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHH
YHAMELPNKMKMGVSGCLLSCSESGVKDIGIVGHNKGWRIFVGGNAGPRPRLGEMLVDNV
HHHHHCCHHHHHCHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHCC
QSEEEVLEIVAKVVDFYKNDPQQGRIGKLVEEMGIEAFRAKVLGTAAAAAGSAK
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TEAMTKKDIPTKGAILQRDRETYAIAPHIPGGFTDTATLRKICDVADKYNLALKITSAQ
CCCCCCCCCCCCCHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCC
RIAMIGAKEEELDAIWSDLAQNPGAAIGLCVRSVKVCPGTQHCKRAVLDSASLGLKLDRI
EEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHH
YHAMELPNKMKMGVSGCLLSCSESGVKDIGIVGHNKGWRIFVGGNAGPRPRLGEMLVDNV
HHHHHCCHHHHHCHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHCC
QSEEEVLEIVAKVVDFYKNDPQQGRIGKLVEEMGIEAFRAKVLGTAAAAAGSAK
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD; NAD+; NADH; Siroheme [C]

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.4 {NO2-}} 0.005 {NO2-}} 0.016 {NADH}} 5.3 {hydroxylamine}} [C]

Substrates: 3 NAD(P)H; nitrite [C]

Specific reaction: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O [C]

General reaction: Redox reaction [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Bipyridine; Arsenite; CN-; NAD+; NADP+; NaN3; p-Chloromercuribenzoate; SO32- [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1911781 [H]