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The map label for this gene is metF-2 [H]

Identifier: 197117010

GI number: 197117010

Start: 733566

End: 735398

Strand: Direct

Name: metF-2 [H]

Synonym: Gbem_0613

Alternate gene names: 197117010

Gene position: 733566-735398 (Clockwise)

Preceding gene: 197117009

Following gene: 197117011

Centisome position: 15.89

GC content: 66.78

Gene sequence:

>1833_bases
ATGGGCGGATCTTTTCTGGAGCGGGTGGCGCATGAGGTGCTGATCGGCGACGGCGCCATAGGGACCATGCTTTATGCGAA
GGGCGTGGCGCTAGACGCCAACTTCGAGCACCTGAACCTGGTGCGGCCGCAACTGGTGCTCGACCTGCACGCCGAGTACC
TGGCGGCGGGAGCGCAGGTGATCGAGACCAACACCTTCGGCGCCAACTACGCCAAGCTCTCCGCCATCGGCATCGGCGGC
AAGGTGGCCGACATCAACCGCCAGGGGGCGATCCTCGCCCGCCGCGCCGCTAAGGTGCGCGACGTCTTCGTCGCCGGTTC
CATGGGACCCATGGGTCGCGGCAAGCAGGAGATGAGCGCCGACCAGGTGCGCGACTCCTTCCGCCTCCAGGCAGCGGCCC
TCGCCGAGGGCGGCGTGGATCTCCTCATCCTGGAAACCTTCTCCGAACTGGACGAGCTGGAAACGGCGCTCTCTGCTGCC
CGCGAGACCGGGCTCCCCGTGGTGGCGAACCTTGCCTTCGGGGAAGGTTCGAGGCTTGCCGGCGGCATCGAGGCCGAGGA
CGCGGCTCTGAGACTCGCCGCGGCCGGCGCGAGCCTCGTGGGCGCCAACTGCGGCGCCGGCCCCCTGGAGCTCCTCGCCA
CCCTGAAGCGGATCGCTGCGGTCACGGACCTTCCCCTGGCCGCCTACCCCAACTCCGGCTTCCCCGAATACGTCGACGGG
CGTTACATCTACCGGACCACCCCCGACTACTTCGCCGCCCGGGCCGAGGAAATGGTCGCGGCAGGCGCCGTCTTGGTGGG
AGGGTGCTGCGGCACCACCCCTGAGCATATCCGGGTCATGGCACAGCGCCTTAAGGGAGCCCGTCCCGCAGCGCGCGTAG
CCGTCGGCGCCGTGAGCCGCACCCCCGCCTCGGAAAAGGCCAAGACTGCAGCCTCCGGTTTTCTCGACCTCTGGGGGAAG
GAGATGGTGGTCACCGTCGAGCTTGACCCACCGAAGGGGCTCGACTGCAGCAGGATACTGGCCGGGAGCCGCGCACTCAA
AGAGGCCGGTGCCGACGCCATCAACCTCGCCGAAAACCCGCTGGCGCGCGTGCGCATGGGGAACCTGGCGCTCGCCTCCC
TGATCCGGCGCGATGTCGGGATCGAGGTCATCGCCCATATCACCTGCCGCGACCGCAACTTGATCGGGCTTCAATCCGAC
CTGATGGGGGCGAGCCTTCTGGGAGTGAGCTCCATCCTCGCCGTAACCGGTGACCCGGCGAGCCTCGGCGAGGAGGCCGG
CGCCTCCTCCGTCTTCGACCTCAACTCCTTCACGCTGATCAAGCTCTTGAGCGACCTGAACCGCGGCGTCAACGCGCTGG
GAAACCCCATCGGCGCCGGCACCGGATTCACCATCGGCGCCGCCTTCAATCCCAACACCCAGAAGATGGAGGTGCAGGTG
GCGCGGCTCGCGAAGAAGGTGGCCAACGGCGCCTGCTTCGCCCAGACCCAGCCCATCTACGACCTGGAGCGGTTCGAGCA
GATGATGGAGCAGACCGCGCACCTGGGGATACCTATCCTACCCGGCGTGCTCCCCTTGGTGAGCGGCCGCAACGCCGAAT
TCCTGCACAACGAAGTCCCCGGCATCACCATTCCTGACGGCATAAGGGCTCGGATGGCGGGTAAGACGGGGGAGGAAGGG
GTACGCGAGGGGCTGGCCATCGCCCGCGAATTCATCGAGGCGACTATGGGGAGGGTGGGTGGATTCTACCTGATACCCCC
CTTCGGGAAGTACGAGATCGCCGTAGAGTTGGTGAAGTTCATAAAATCAAGGGCGGAACACAGAGGTCACTGA

Upstream 100 bases:

>100_bases
TACCCCCAGCCCGAGCCGCAGGTATTCGAGATGAAAGGGGTCATGTCGCGCAAGAAACAGCTGGTGCCGCACCTGATCAA
GGTGCTGGGGGGACGCTAAC

Downstream 100 bases:

>100_bases
GGTCACAGAGGAAGGCGAAAAACTTTTGGTTTAACCCAAAAACGCCTTTGCTTTTGGATTTTCCTCTGTGTACCTCTGTG
TCCTCTGTGGTGAGTGCCTT

Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 610; Mature: 609

Protein sequence:

>610_residues
MGGSFLERVAHEVLIGDGAIGTMLYAKGVALDANFEHLNLVRPQLVLDLHAEYLAAGAQVIETNTFGANYAKLSAIGIGG
KVADINRQGAILARRAAKVRDVFVAGSMGPMGRGKQEMSADQVRDSFRLQAAALAEGGVDLLILETFSELDELETALSAA
RETGLPVVANLAFGEGSRLAGGIEAEDAALRLAAAGASLVGANCGAGPLELLATLKRIAAVTDLPLAAYPNSGFPEYVDG
RYIYRTTPDYFAARAEEMVAAGAVLVGGCCGTTPEHIRVMAQRLKGARPAARVAVGAVSRTPASEKAKTAASGFLDLWGK
EMVVTVELDPPKGLDCSRILAGSRALKEAGADAINLAENPLARVRMGNLALASLIRRDVGIEVIAHITCRDRNLIGLQSD
LMGASLLGVSSILAVTGDPASLGEEAGASSVFDLNSFTLIKLLSDLNRGVNALGNPIGAGTGFTIGAAFNPNTQKMEVQV
ARLAKKVANGACFAQTQPIYDLERFEQMMEQTAHLGIPILPGVLPLVSGRNAEFLHNEVPGITIPDGIRARMAGKTGEEG
VREGLAIAREFIEATMGRVGGFYLIPPFGKYEIAVELVKFIKSRAEHRGH

Sequences:

>Translated_610_residues
MGGSFLERVAHEVLIGDGAIGTMLYAKGVALDANFEHLNLVRPQLVLDLHAEYLAAGAQVIETNTFGANYAKLSAIGIGG
KVADINRQGAILARRAAKVRDVFVAGSMGPMGRGKQEMSADQVRDSFRLQAAALAEGGVDLLILETFSELDELETALSAA
RETGLPVVANLAFGEGSRLAGGIEAEDAALRLAAAGASLVGANCGAGPLELLATLKRIAAVTDLPLAAYPNSGFPEYVDG
RYIYRTTPDYFAARAEEMVAAGAVLVGGCCGTTPEHIRVMAQRLKGARPAARVAVGAVSRTPASEKAKTAASGFLDLWGK
EMVVTVELDPPKGLDCSRILAGSRALKEAGADAINLAENPLARVRMGNLALASLIRRDVGIEVIAHITCRDRNLIGLQSD
LMGASLLGVSSILAVTGDPASLGEEAGASSVFDLNSFTLIKLLSDLNRGVNALGNPIGAGTGFTIGAAFNPNTQKMEVQV
ARLAKKVANGACFAQTQPIYDLERFEQMMEQTAHLGIPILPGVLPLVSGRNAEFLHNEVPGITIPDGIRARMAGKTGEEG
VREGLAIAREFIEATMGRVGGFYLIPPFGKYEIAVELVKFIKSRAEHRGH
>Mature_609_residues
GGSFLERVAHEVLIGDGAIGTMLYAKGVALDANFEHLNLVRPQLVLDLHAEYLAAGAQVIETNTFGANYAKLSAIGIGGK
VADINRQGAILARRAAKVRDVFVAGSMGPMGRGKQEMSADQVRDSFRLQAAALAEGGVDLLILETFSELDELETALSAAR
ETGLPVVANLAFGEGSRLAGGIEAEDAALRLAAAGASLVGANCGAGPLELLATLKRIAAVTDLPLAAYPNSGFPEYVDGR
YIYRTTPDYFAARAEEMVAAGAVLVGGCCGTTPEHIRVMAQRLKGARPAARVAVGAVSRTPASEKAKTAASGFLDLWGKE
MVVTVELDPPKGLDCSRILAGSRALKEAGADAINLAENPLARVRMGNLALASLIRRDVGIEVIAHITCRDRNLIGLQSDL
MGASLLGVSSILAVTGDPASLGEEAGASSVFDLNSFTLIKLLSDLNRGVNALGNPIGAGTGFTIGAAFNPNTQKMEVQVA
RLAKKVANGACFAQTQPIYDLERFEQMMEQTAHLGIPILPGVLPLVSGRNAEFLHNEVPGITIPDGIRARMAGKTGEEGV
REGLAIAREFIEATMGRVGGFYLIPPFGKYEIAVELVKFIKSRAEHRGH

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=317, Percent_Identity=27.1293375394322, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI13162290, Length=342, Percent_Identity=31.2865497076023, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI157266337, Length=348, Percent_Identity=31.6091954022989, Blast_Score=109, Evalue=7e-24,
Organism=Escherichia coli, GI1790450, Length=320, Percent_Identity=28.125, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786456, Length=305, Percent_Identity=32.4590163934426, Blast_Score=91, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17535405, Length=328, Percent_Identity=29.5731707317073, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 64000; Mature: 63869

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGSFLERVAHEVLIGDGAIGTMLYAKGVALDANFEHLNLVRPQLVLDLHAEYLAAGAQV
CCHHHHHHHHHHHHCCCCCHHHHHHHCCEEEECCCCHHHHCCHHHHHHHHHHHHHCCCCE
IETNTFGANYAKLSAIGIGGKVADINRQGAILARRAAKVRDVFVAGSMGPMGRGKQEMSA
EEECCCCCCCCEEEEEECCCEEECCCCCCHHHHHHHHHHHHEEEECCCCCCCCCHHHHHH
DQVRDSFRLQAAALAEGGVDLLILETFSELDELETALSAARETGLPVVANLAFGEGSRLA
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GGIEAEDAALRLAAAGASLVGANCGAGPLELLATLKRIAAVTDLPLAAYPNSGFPEYVDG
CCCCCCHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCC
RYIYRTTPDYFAARAEEMVAAGAVLVGGCCGTTPEHIRVMAQRLKGARPAARVAVGAVSR
EEEEECCCHHHHHHHHHHHHHCHHEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCC
TPASEKAKTAASGFLDLWGKEMVVTVELDPPKGLDCSRILAGSRALKEAGADAINLAENP
CCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEHHCCCC
LARVRMGNLALASLIRRDVGIEVIAHITCRDRNLIGLQSDLMGASLLGVSSILAVTGDPA
HHHHCCCHHHHHHHHHHHCCEEEEEEEEECCCCEECCHHHHHHHHHHHHHHHHEECCCCH
SLGEEAGASSVFDLNSFTLIKLLSDLNRGVNALGNPIGAGTGFTIGAAFNPNTQKMEVQV
HHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHH
ARLAKKVANGACFAQTQPIYDLERFEQMMEQTAHLGIPILPGVLPLVSGRNAEFLHNEVP
HHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHCCCC
GITIPDGIRARMAGKTGEEGVREGLAIAREFIEATMGRVGGFYLIPPFGKYEIAVELVKF
CCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
IKSRAEHRGH
HHHHHHHCCC
>Mature Secondary Structure 
GGSFLERVAHEVLIGDGAIGTMLYAKGVALDANFEHLNLVRPQLVLDLHAEYLAAGAQV
CHHHHHHHHHHHHCCCCCHHHHHHHCCEEEECCCCHHHHCCHHHHHHHHHHHHHCCCCE
IETNTFGANYAKLSAIGIGGKVADINRQGAILARRAAKVRDVFVAGSMGPMGRGKQEMSA
EEECCCCCCCCEEEEEECCCEEECCCCCCHHHHHHHHHHHHEEEECCCCCCCCCHHHHHH
DQVRDSFRLQAAALAEGGVDLLILETFSELDELETALSAARETGLPVVANLAFGEGSRLA
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GGIEAEDAALRLAAAGASLVGANCGAGPLELLATLKRIAAVTDLPLAAYPNSGFPEYVDG
CCCCCCHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCC
RYIYRTTPDYFAARAEEMVAAGAVLVGGCCGTTPEHIRVMAQRLKGARPAARVAVGAVSR
EEEEECCCHHHHHHHHHHHHHCHHEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCC
TPASEKAKTAASGFLDLWGKEMVVTVELDPPKGLDCSRILAGSRALKEAGADAINLAENP
CCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEHHCCCC
LARVRMGNLALASLIRRDVGIEVIAHITCRDRNLIGLQSDLMGASLLGVSSILAVTGDPA
HHHHCCCHHHHHHHHHHHCCEEEEEEEEECCCCEECCHHHHHHHHHHHHHHHHEECCCCH
SLGEEAGASSVFDLNSFTLIKLLSDLNRGVNALGNPIGAGTGFTIGAAFNPNTQKMEVQV
HHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHH
ARLAKKVANGACFAQTQPIYDLERFEQMMEQTAHLGIPILPGVLPLVSGRNAEFLHNEVP
HHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHCCCC
GITIPDGIRARMAGKTGEEGVREGLAIAREFIEATMGRVGGFYLIPPFGKYEIAVELVKF
CCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
IKSRAEHRGH
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]