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The map label for this gene is ttcA

Identifier: 197117012

GI number: 197117012

Start: 736306

End: 737073

Strand: Direct

Name: ttcA

Synonym: Gbem_0615

Alternate gene names: 197117012

Gene position: 736306-737073 (Clockwise)

Preceding gene: 197117011

Following gene: 197117013

Centisome position: 15.95

GC content: 61.98

Gene sequence:

>768_bases
GTGGCGCTTATTGAGGACGCCGCCTTCACCCGGATCAAGAACCGGGTGGGGAAGGCCATAGCGGAGTTCGACCTGATCTC
CGAGGGGGACCGGGTCGCGGTGGCGGTATCGGGGGGGAAGGATTCCTACACCATGCTGCACATGCTGGAGACCCTGAGAA
GGCGCGCGCCGGTACGCTACGAGCTGGTGGCGATCAACATCGACTCCGGCTACCGCGGCTACCGCGCCGACATCATCGAG
GAGCACCTGCGCGAGCACGGCTTCACCTATCACATGGAGAAGACCGACCACTACGACATCATCTCCGAGAAGCGGCGCCC
CAATTCCTCGTACTGCTCCATCTGCGCCAGGCTGAAGCGGGGGACGCTCTACACCCTGGCCCAGCAGTACGGCTGCAACA
AGCTCGCGCTCGGGCACCACATGGATGACTTCATCGAGACCCTGCTCCTGAACCAGTTCTTCGTAGGCTCCCTGAAGGCG
ATGGCCCCGAGCATGCTGGCCGACAACGGCGTCACCACCGTGATCAGGCCTTTGGTTTACGTGCCGGAGAAGGAGATAAT
CCCGTTCTCGCGCAACAACCGCTTCCCCGTGGTCTGCTGCTGTTGCCCGGTCTGCGGCACCGCCGACCTGCAGCGAAAGA
AGATGAAAGAGTTGCTGGAGACCTTGGAGCGGGACAACCCCCTGGTGAAGAAGAGCCTTTTGACCGCGCTCTCCAACGTG
CATCCGCGCCATTTGCTGGACAAGGGGTTGACTAGGAAGCCTTCCTGA

Upstream 100 bases:

>100_bases
TCCGTGCCAAGGTCCTGGGAACCGCTGCCGCAGCGGCAGGGAGCGCCAAGTAATATGGACGCAAGTACGCAGGATCAGGC
GCTCTCCGCGGGAGGTTGCC

Downstream 100 bases:

>100_bases
CGCAGATCGGCGACGATGTCCTTCACCGTCAGGTAGCCGCAGTAGATAACTATGGGAACTGCTCCTATGACCATCGGTTC
CATGTTTGCCACCTCCATCC

Product: tRNA (2-thio-C32)-thioltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MALIEDAAFTRIKNRVGKAIAEFDLISEGDRVAVAVSGGKDSYTMLHMLETLRRRAPVRYELVAINIDSGYRGYRADIIE
EHLREHGFTYHMEKTDHYDIISEKRRPNSSYCSICARLKRGTLYTLAQQYGCNKLALGHHMDDFIETLLLNQFFVGSLKA
MAPSMLADNGVTTVIRPLVYVPEKEIIPFSRNNRFPVVCCCCPVCGTADLQRKKMKELLETLERDNPLVKKSLLTALSNV
HPRHLLDKGLTRKPS

Sequences:

>Translated_255_residues
MALIEDAAFTRIKNRVGKAIAEFDLISEGDRVAVAVSGGKDSYTMLHMLETLRRRAPVRYELVAINIDSGYRGYRADIIE
EHLREHGFTYHMEKTDHYDIISEKRRPNSSYCSICARLKRGTLYTLAQQYGCNKLALGHHMDDFIETLLLNQFFVGSLKA
MAPSMLADNGVTTVIRPLVYVPEKEIIPFSRNNRFPVVCCCCPVCGTADLQRKKMKELLETLERDNPLVKKSLLTALSNV
HPRHLLDKGLTRKPS
>Mature_254_residues
ALIEDAAFTRIKNRVGKAIAEFDLISEGDRVAVAVSGGKDSYTMLHMLETLRRRAPVRYELVAINIDSGYRGYRADIIEE
HLREHGFTYHMEKTDHYDIISEKRRPNSSYCSICARLKRGTLYTLAQQYGCNKLALGHHMDDFIETLLLNQFFVGSLKAM
APSMLADNGVTTVIRPLVYVPEKEIIPFSRNNRFPVVCCCCPVCGTADLQRKKMKELLETLERDNPLVKKSLLTALSNVH
PRHLLDKGLTRKPS

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=241, Percent_Identity=41.0788381742739, Blast_Score=207, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI193211090, Length=243, Percent_Identity=35.3909465020576, Blast_Score=136, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17540090, Length=254, Percent_Identity=28.740157480315, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24651972, Length=244, Percent_Identity=29.5081967213115, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_GEOBB (B5ED57)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137439.1
- GeneID:   6783448
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0615
- HOGENOM:   HBG347038
- OMA:   EHILPEY
- ProtClustDB:   PRK10696
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 28944; Mature: 28813

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIEDAAFTRIKNRVGKAIAEFDLISEGDRVAVAVSGGKDSYTMLHMLETLRRRAPVRY
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEE
ELVAINIDSGYRGYRADIIEEHLREHGFTYHMEKTDHYDIISEKRRPNSSYCSICARLKR
EEEEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHC
GTLYTLAQQYGCNKLALGHHMDDFIETLLLNQFFVGSLKAMAPSMLADNGVTTVIRPLVY
CHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
VPEKEIIPFSRNNRFPVVCCCCPVCGTADLQRKKMKELLETLERDNPLVKKSLLTALSNV
CCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
HPRHLLDKGLTRKPS
CHHHHHHCCCCCCCC
>Mature Secondary Structure 
ALIEDAAFTRIKNRVGKAIAEFDLISEGDRVAVAVSGGKDSYTMLHMLETLRRRAPVRY
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEE
ELVAINIDSGYRGYRADIIEEHLREHGFTYHMEKTDHYDIISEKRRPNSSYCSICARLKR
EEEEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHC
GTLYTLAQQYGCNKLALGHHMDDFIETLLLNQFFVGSLKAMAPSMLADNGVTTVIRPLVY
CHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
VPEKEIIPFSRNNRFPVVCCCCPVCGTADLQRKKMKELLETLERDNPLVKKSLLTALSNV
CCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
HPRHLLDKGLTRKPS
CHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA