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The map label for this gene is hisZ [H]

Identifier: 197116951

GI number: 197116951

Start: 665916

End: 667238

Strand: Reverse

Name: hisZ [H]

Synonym: Gbem_0553

Alternate gene names: 197116951

Gene position: 667238-665916 (Counterclockwise)

Preceding gene: 197116952

Following gene: 197116950

Centisome position: 14.46

GC content: 61.9

Gene sequence:

>1323_bases
GTGACTAACCCCTCATGTATTGAAGCTCCGCTCCCCAAAGGGGTGAGCGACTTCCTCCCGGAAACCGCCGACAAGATCAC
CTTTATCGCGGACAGCATCCACCGAGTCTTCGAACTCTGGGGCTTCAGGCGCATGATCACCCCGCTGCTCGAGTTCGAGC
ACGTGCTCGCTCTGGGCATGGGTGACGAACTGCGGAGCAAGACCTTCCGCTTCGACGACCGCCAGACCGGACGCCTCCTC
GCCATTCCGCCGGACATCACGCCGCAGGTGGCACGCATCGTCGCCACCCGGATGCACGCCCTACCCCTCCCCCACCGCAT
ATACTATTCCGGACGCGTGCTCAGGCAGGCCCAGATGCAATCGGGCAAAAGCCGCGAGATCTTCCAGTTCGGCGTAGAGC
TGATCGGGCTGGACTCCCCCGAGGCGGACGCCGAGATGGTGGCGATGGCGGTGGAGGTGCTGAAAAACCTCGGCTTCACC
GGCTTCAAGATAGACCTGGGGCAGGTGGAGTTCTACCGCGGCATCATGGACGCCTCCGGCCTCTCCGCCGAGGTGCAGAA
ACAGTTGCAGGAAGCGATCAGCAAGAAGGAAGTCAGCGCGGTGCGCAGCATCCTCGAATCCGCCGGGGCTCCCGACCGGG
TCAAGCAGGAGATCGCCCTGCTGCCGAGACTCTACGGCGGGCGCGAGGTGTTGCAGGAAGCGCGCAGTATCGCCGGAAAC
GAGCGTTCGCTGCGGGCCCTGGACAACCTCGCCCAGGTGGTCGACATCTTGGACATATACGGGGTCGCCGAGCATCTAAC
CATCGACCTGGGCGAGATCCGGGGGCTGGACTACCACAGCGGGATCACCTTCGAAGGCTTCGTCCCGGGCGTCGGAGAAG
CGATCTGCAGCGGCGGGCGTTACGACGACCTCACCGCCAAGTACGGATACCCGGCGCATGCCACCGGCTTCGCTTTCAAC
ATCCTGGCCCTTCTGGCCAGCATGTCCAAGAGGCCTGAGGTCGAGGCGTCAAGCAGCCGCGACTTCCTGATCTTCAACAG
AAAGGACGAGCGCCGCGAGGCGCTGGAAGTGGCGCAAAAGCTGAGAAGCCTAGGCTACACCTGCGCCAGGGATATCATCA
AGCGCGACTTCGACAGTTCGCTTGAGTACGCAAAGAAGATGAACATCCGATTGCTGCTTGTGATCGGCGCTGAGGATTGC
GCGGCCGGCCAGCTTTACCTGGTGCGGGTGGCGGACCGTCAAAGCATCACCGTGAGCGAAGAGGAGCTGTTCGATAAGGA
TCTAGATTTGAAATTCGATCTGCAAGGGGAGAATCATGGCTAA

Upstream 100 bases:

>100_bases
TGGCAGGGGGAATTAATCGTTGACTTCCCCCTGCCATACTGTTACGATTTCTCGTTTTTTCGCCAGCTTCAGCGGTACCG
ACATACCTTAGGACGACCCC

Downstream 100 bases:

>100_bases
CGTCGTTGTAATAGGTGCCCAGTGGGGCGATGAAGGCAAGGGTAAGGTCGTAGACATTTACACGGAGTTCGCGGACAACG
TGGTGCGTTACCAGGGCGGG

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MTNPSCIEAPLPKGVSDFLPETADKITFIADSIHRVFELWGFRRMITPLLEFEHVLALGMGDELRSKTFRFDDRQTGRLL
AIPPDITPQVARIVATRMHALPLPHRIYYSGRVLRQAQMQSGKSREIFQFGVELIGLDSPEADAEMVAMAVEVLKNLGFT
GFKIDLGQVEFYRGIMDASGLSAEVQKQLQEAISKKEVSAVRSILESAGAPDRVKQEIALLPRLYGGREVLQEARSIAGN
ERSLRALDNLAQVVDILDIYGVAEHLTIDLGEIRGLDYHSGITFEGFVPGVGEAICSGGRYDDLTAKYGYPAHATGFAFN
ILALLASMSKRPEVEASSSRDFLIFNRKDERREALEVAQKLRSLGYTCARDIIKRDFDSSLEYAKKMNIRLLLVIGAEDC
AAGQLYLVRVADRQSITVSEEELFDKDLDLKFDLQGENHG

Sequences:

>Translated_440_residues
MTNPSCIEAPLPKGVSDFLPETADKITFIADSIHRVFELWGFRRMITPLLEFEHVLALGMGDELRSKTFRFDDRQTGRLL
AIPPDITPQVARIVATRMHALPLPHRIYYSGRVLRQAQMQSGKSREIFQFGVELIGLDSPEADAEMVAMAVEVLKNLGFT
GFKIDLGQVEFYRGIMDASGLSAEVQKQLQEAISKKEVSAVRSILESAGAPDRVKQEIALLPRLYGGREVLQEARSIAGN
ERSLRALDNLAQVVDILDIYGVAEHLTIDLGEIRGLDYHSGITFEGFVPGVGEAICSGGRYDDLTAKYGYPAHATGFAFN
ILALLASMSKRPEVEASSSRDFLIFNRKDERREALEVAQKLRSLGYTCARDIIKRDFDSSLEYAKKMNIRLLLVIGAEDC
AAGQLYLVRVADRQSITVSEEELFDKDLDLKFDLQGENHG
>Mature_439_residues
TNPSCIEAPLPKGVSDFLPETADKITFIADSIHRVFELWGFRRMITPLLEFEHVLALGMGDELRSKTFRFDDRQTGRLLA
IPPDITPQVARIVATRMHALPLPHRIYYSGRVLRQAQMQSGKSREIFQFGVELIGLDSPEADAEMVAMAVEVLKNLGFTG
FKIDLGQVEFYRGIMDASGLSAEVQKQLQEAISKKEVSAVRSILESAGAPDRVKQEIALLPRLYGGREVLQEARSIAGNE
RSLRALDNLAQVVDILDIYGVAEHLTIDLGEIRGLDYHSGITFEGFVPGVGEAICSGGRYDDLTAKYGYPAHATGFAFNI
LALLASMSKRPEVEASSSRDFLIFNRKDERREALEVAQKLRSLGYTCARDIIKRDFDSSLEYAKKMNIRLLLVIGAEDCA
AGQLYLVRVADRQSITVSEEELFDKDLDLKFDLQGENHG

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=444, Percent_Identity=23.4234234234234, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI6996014, Length=448, Percent_Identity=24.1071428571429, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1788861, Length=406, Percent_Identity=24.8768472906404, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71993686, Length=452, Percent_Identity=23.4513274336283, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71993693, Length=452, Percent_Identity=23.4513274336283, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325290, Length=461, Percent_Identity=24.0780911062907, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24643061, Length=457, Percent_Identity=23.4135667396061, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24643059, Length=474, Percent_Identity=22.3628691983122, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR004517
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 48976; Mature: 48845

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPSCIEAPLPKGVSDFLPETADKITFIADSIHRVFELWGFRRMITPLLEFEHVLALGM
CCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDELRSKTFRFDDRQTGRLLAIPPDITPQVARIVATRMHALPLPHRIYYSGRVLRQAQMQ
CHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHEECCCHHHHHHHHH
SGKSREIFQFGVELIGLDSPEADAEMVAMAVEVLKNLGFTGFKIDLGQVEFYRGIMDASG
CCCCHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCC
LSAEVQKQLQEAISKKEVSAVRSILESAGAPDRVKQEIALLPRLYGGREVLQEARSIAGN
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
ERSLRALDNLAQVVDILDIYGVAEHLTIDLGEIRGLDYHSGITFEGFVPGVGEAICSGGR
CHHHHHHHHHHHHHHHHHHHCCHHHHEEEHHHHCCCCCCCCCEECCCCCCHHHHHHCCCC
YDDLTAKYGYPAHATGFAFNILALLASMSKRPEVEASSSRDFLIFNRKDERREALEVAQK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHH
LRSLGYTCARDIIKRDFDSSLEYAKKMNIRLLLVIGAEDCAAGQLYLVRVADRQSITVSE
HHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEEEECCCCCEECH
EELFDKDLDLKFDLQGENHG
HHHHCCCCCEEEEECCCCCC
>Mature Secondary Structure 
TNPSCIEAPLPKGVSDFLPETADKITFIADSIHRVFELWGFRRMITPLLEFEHVLALGM
CCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDELRSKTFRFDDRQTGRLLAIPPDITPQVARIVATRMHALPLPHRIYYSGRVLRQAQMQ
CHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHEECCCHHHHHHHHH
SGKSREIFQFGVELIGLDSPEADAEMVAMAVEVLKNLGFTGFKIDLGQVEFYRGIMDASG
CCCCHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCC
LSAEVQKQLQEAISKKEVSAVRSILESAGAPDRVKQEIALLPRLYGGREVLQEARSIAGN
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
ERSLRALDNLAQVVDILDIYGVAEHLTIDLGEIRGLDYHSGITFEGFVPGVGEAICSGGR
CHHHHHHHHHHHHHHHHHHHCCHHHHEEEHHHHCCCCCCCCCEECCCCCCHHHHHHCCCC
YDDLTAKYGYPAHATGFAFNILALLASMSKRPEVEASSSRDFLIFNRKDERREALEVAQK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHH
LRSLGYTCARDIIKRDFDSSLEYAKKMNIRLLLVIGAEDCAAGQLYLVRVADRQSITVSE
HHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEEEECCCCCEECH
EELFDKDLDLKFDLQGENHG
HHHHCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA