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The map label for this gene is purA

Identifier: 197116950

GI number: 197116950

Start: 664625

End: 665923

Strand: Reverse

Name: purA

Synonym: Gbem_0552

Alternate gene names: 197116950

Gene position: 665923-664625 (Counterclockwise)

Preceding gene: 197116951

Following gene: 197116948

Centisome position: 14.43

GC content: 62.89

Gene sequence:

>1299_bases
ATGGCTAACGTCGTTGTAATAGGTGCCCAGTGGGGCGATGAAGGCAAGGGTAAGGTCGTAGACATTTACACGGAGTTCGC
GGACAACGTGGTGCGTTACCAGGGCGGGAACAACGCCGGCCACACGCTGGTCGTGGGCGACGAAAAGGTCATCCTGCACC
TGATCCCGTCCGGGATTCTGCACGAAGGGAAGCGCTGCGTGATCGGCAACGGGGTGGTGCTCGACCCGGAAGTCTTCATC
ATGGAGATCACCAAGCTCAAGGCCAACGGCTACCTCAAAGACGACAAGATGCTGCTCCTCTCCGAGGCGCTGCACATCAT
CATGCCGTACCACAAGAGGATAGACATCGCCCGCGAGAAAAAGAGCGGCTCCAAGAAGATCGGCACCACCGGGCGCGGCA
TCGGCCCCGCCTACGAGGACAAGATCGGGCGCCGCGGCATCCGTCTCATGGACCTTCTCGACGAGAAAGCCTTCACCCGC
AAGGTGAAAGAGGTGCTCGAGGAAAAGAACCTGATCCTGACCCAGCTTCTGGGCGACCAGCCGTTCACCTTCGAGGAGAT
CTACGAAGAGTACATGAAGTACGCCGAGACCCTCAGGAAATACGCGGCCGACACCTCGCTCATCCTGCACCAGGAGACCA
AGGCGGGCAAAAGCCTCCTCTTCGAAGGAGCGCAGGGAACACTCCTCGACGTCGACCACGGCACCTACCCCTACGTTACC
TCCTCTTCCACCTGCTCCGGCGGCGCCTGCACCGGAAGCGGCGTCTCCCCGAGGGAGATCCACGAGGTGATCGGCATCTC
CAAGGCCTACGCCACCCGCGTGGGGAGCGGCCCGTTCCCGACCGAACTTGAGGACGAGACCGGCGAGCAGCTGCGCCAGG
CAGGCCGCGAGTTCGGCTCCACCACCGGGCGCCCGCGCCGCACCGGCTGGTACGACGCGCTGGTCGCGCGCTACGCCGTC
AGGGTCAACGGCCTCTCCGGCATCGCCATCACCAAGCTCGACGTCCTCTCCGGGCAGGAAACGGTCAAGGTCTGCACCGC
TTACAACTATAAGGGTCAGGTACTCACCGAGGTCCCGGCAAGCCTCGAGATCATGGAACAGTGCACCCCGATCTATGAGG
AACTCCCCGGCTGGAACGACGACATCACCGGCGCGAAGAGCATGGCCGAGCTCCCGAAGAACGCCCGCGACTACGTGGCG
CGCATCGAGAAGCTCTCCGGCGCCCCCGTGGTACTGGTCTCCGTCGGCCCGCGCCGTGACGAGACTATCGTACTCAGAAA
CCCGTTCGAGCTGAACTAA

Upstream 100 bases:

>100_bases
TACCTGGTGCGGGTGGCGGACCGTCAAAGCATCACCGTGAGCGAAGAGGAGCTGTTCGATAAGGATCTAGATTTGAAATT
CGATCTGCAAGGGGAGAATC

Downstream 100 bases:

>100_bases
CTTTTTAATAAAAAGTGTTGACTGGCATAAATCGATTTGCTATAAGGTTGTTCTTGCTTCGCTGCACTGTTTGAGCCGCT
AGCTCAGTTGGTAGAGCACC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MANVVVIGAQWGDEGKGKVVDIYTEFADNVVRYQGGNNAGHTLVVGDEKVILHLIPSGILHEGKRCVIGNGVVLDPEVFI
MEITKLKANGYLKDDKMLLLSEALHIIMPYHKRIDIAREKKSGSKKIGTTGRGIGPAYEDKIGRRGIRLMDLLDEKAFTR
KVKEVLEEKNLILTQLLGDQPFTFEEIYEEYMKYAETLRKYAADTSLILHQETKAGKSLLFEGAQGTLLDVDHGTYPYVT
SSSTCSGGACTGSGVSPREIHEVIGISKAYATRVGSGPFPTELEDETGEQLRQAGREFGSTTGRPRRTGWYDALVARYAV
RVNGLSGIAITKLDVLSGQETVKVCTAYNYKGQVLTEVPASLEIMEQCTPIYEELPGWNDDITGAKSMAELPKNARDYVA
RIEKLSGAPVVLVSVGPRRDETIVLRNPFELN

Sequences:

>Translated_432_residues
MANVVVIGAQWGDEGKGKVVDIYTEFADNVVRYQGGNNAGHTLVVGDEKVILHLIPSGILHEGKRCVIGNGVVLDPEVFI
MEITKLKANGYLKDDKMLLLSEALHIIMPYHKRIDIAREKKSGSKKIGTTGRGIGPAYEDKIGRRGIRLMDLLDEKAFTR
KVKEVLEEKNLILTQLLGDQPFTFEEIYEEYMKYAETLRKYAADTSLILHQETKAGKSLLFEGAQGTLLDVDHGTYPYVT
SSSTCSGGACTGSGVSPREIHEVIGISKAYATRVGSGPFPTELEDETGEQLRQAGREFGSTTGRPRRTGWYDALVARYAV
RVNGLSGIAITKLDVLSGQETVKVCTAYNYKGQVLTEVPASLEIMEQCTPIYEELPGWNDDITGAKSMAELPKNARDYVA
RIEKLSGAPVVLVSVGPRRDETIVLRNPFELN
>Mature_431_residues
ANVVVIGAQWGDEGKGKVVDIYTEFADNVVRYQGGNNAGHTLVVGDEKVILHLIPSGILHEGKRCVIGNGVVLDPEVFIM
EITKLKANGYLKDDKMLLLSEALHIIMPYHKRIDIAREKKSGSKKIGTTGRGIGPAYEDKIGRRGIRLMDLLDEKAFTRK
VKEVLEEKNLILTQLLGDQPFTFEEIYEEYMKYAETLRKYAADTSLILHQETKAGKSLLFEGAQGTLLDVDHGTYPYVTS
SSTCSGGACTGSGVSPREIHEVIGISKAYATRVGSGPFPTELEDETGEQLRQAGREFGSTTGRPRRTGWYDALVARYAVR
VNGLSGIAITKLDVLSGQETVKVCTAYNYKGQVLTEVPASLEIMEQCTPIYEELPGWNDDITGAKSMAELPKNARDYVAR
IEKLSGAPVVLVSVGPRRDETIVLRNPFELN

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=42.2897196261682, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI22748717, Length=427, Percent_Identity=41.4519906323185, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=39.4472361809045, Blast_Score=277, Evalue=1e-74,
Organism=Escherichia coli, GI1790620, Length=429, Percent_Identity=53.1468531468531, Blast_Score=461, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=43.1235431235431, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=42.6573426573427, Blast_Score=332, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6324109, Length=433, Percent_Identity=42.0323325635104, Blast_Score=341, Evalue=1e-94,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=42.0427553444181, Blast_Score=320, Evalue=9e-88,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_GEOBB (B5ECF3)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137377.1
- GeneID:   6783385
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0552
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- ProtClustDB:   PRK01117
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47487; Mature: 47356

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVIGAQWGDEGKGKVVDIYTEFADNVVRYQGGNNAGHTLVVGDEKVILHLIPSGIL
CCCEEEEEECCCCCCCCCEEEEEHHHHCCEEEECCCCCCCCEEEECCCEEEEEECCCHHH
HEGKRCVIGNGVVLDPEVFIMEITKLKANGYLKDDKMLLLSEALHIIMPYHKRIDIAREK
HCCCEEEEECCEEECCCEEEEEEEEHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
KSGSKKIGTTGRGIGPAYEDKIGRRGIRLMDLLDEKAFTRKVKEVLEEKNLILTQLLGDQ
CCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
PFTFEEIYEEYMKYAETLRKYAADTSLILHQETKAGKSLLFEGAQGTLLDVDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHEEECCCCCEEECCCCCCCEEE
SSSTCSGGACTGSGVSPREIHEVIGISKAYATRVGSGPFPTELEDETGEQLRQAGREFGS
CCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
TTGRPRRTGWYDALVARYAVRVNGLSGIAITKLDVLSGQETVKVCTAYNYKGQVLTEVPA
CCCCCCCCCHHHHHHHHHHHEECCCCCEEEEEEEHHCCCCCEEEEEEECCCCCEEECCCH
SLEIMEQCTPIYEELPGWNDDITGAKSMAELPKNARDYVARIEKLSGAPVVLVSVGPRRD
HHHHHHHCCHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCC
ETIVLRNPFELN
CEEEECCCCCCC
>Mature Secondary Structure 
ANVVVIGAQWGDEGKGKVVDIYTEFADNVVRYQGGNNAGHTLVVGDEKVILHLIPSGIL
CCEEEEEECCCCCCCCCEEEEEHHHHCCEEEECCCCCCCCEEEECCCEEEEEECCCHHH
HEGKRCVIGNGVVLDPEVFIMEITKLKANGYLKDDKMLLLSEALHIIMPYHKRIDIAREK
HCCCEEEEECCEEECCCEEEEEEEEHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
KSGSKKIGTTGRGIGPAYEDKIGRRGIRLMDLLDEKAFTRKVKEVLEEKNLILTQLLGDQ
CCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
PFTFEEIYEEYMKYAETLRKYAADTSLILHQETKAGKSLLFEGAQGTLLDVDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHEEECCCCCEEECCCCCCCEEE
SSSTCSGGACTGSGVSPREIHEVIGISKAYATRVGSGPFPTELEDETGEQLRQAGREFGS
CCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
TTGRPRRTGWYDALVARYAVRVNGLSGIAITKLDVLSGQETVKVCTAYNYKGQVLTEVPA
CCCCCCCCCHHHHHHHHHHHEECCCCCEEEEEEEHHCCCCCEEEEEEECCCCCEEECCCH
SLEIMEQCTPIYEELPGWNDDITGAKSMAELPKNARDYVARIEKLSGAPVVLVSVGPRRD
HHHHHHHCCHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCC
ETIVLRNPFELN
CEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA