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The map label for this gene is murE [H]

Identifier: 197116884

GI number: 197116884

Start: 586176

End: 587693

Strand: Direct

Name: murE [H]

Synonym: Gbem_0486

Alternate gene names: 197116884

Gene position: 586176-587693 (Clockwise)

Preceding gene: 197116883

Following gene: 197116885

Centisome position: 12.7

GC content: 65.55

Gene sequence:

>1518_bases
ATGAAACTCGCACAACTTCTGAATTGCCTCGCCTCGCCCGAGCTCTCCGGAGACGCCGGTATCGAGATAGAGTCGCTTTG
CTACGACTCGCGAAAGGTGGCGCCCGGAGCCCTCTTCTTCGCCCTGCGCGGAGTGAAGAGCGACGGGACCGAATTCGTTG
AAGCCGCTGTAAAGGGGGGGGCGGTCGCAATCGTTGCGGACCGCCCCTGCGCTTGTCCCGGGGTAACGGTGGTCGTGGTC
CCGGACCCAAGGCTCGCCATGAGCCTCATGGCAGCGGCCTTCTACGGCACCCCGACCCGCGGCATCCCGGTGGTAGGGAT
CACCGGGACCAACGGCAAGACCACCACCACCTATCTCGTCGAGGGGATCATGGAGCAGGCGGGAGTGCCGGCAGCTGTCC
TCGGCACCATCAGCTACCGCTTCGGAGAGACCAACATCCCCGCCCCCAACACCACCCCCGAATCGGTCGACCTGCAAGCC
ATCCTGAGGGAACTGGTGGACCACGGCGCGCGCGGCGCGGTGATGGAGGTATCCTCCCACGCCCTGGAACAGCACCGCGT
CGACGGCTGCCTCTTCGACGTCGCCCTCTTCTCCAACCTGACCCGCGACCACCTCGATTACCACCTCGACATGGAGTCCT
ACTTCAAGAGCAAGCTGAGGCTCTTCACCGATCTTCTGGCGCCTACCGCGGAAAAGCCGCTCAGGCGCGCGGTGGTGAAC
CTGGACGACCCCTTCGGCCCTCGCATCGCGGAAAAGGCAGCCGCGCCGGTATTGACCTACTCCATGACCGGGAAGGCCGA
CATCAGCGTGGCCGAGGCCGAATTCTCGGTCGACGGCATCCGCTGCCGCCTCAACACCCCGGTAGGGGAGATCTCCCTTG
CCTCAGAGCTTTTGGGGCGCTTCAACCTCTACAACATCCTCTCCGCGGTAGGGGCGGGTCTTGCCCTTGGCTACTCGAAG
GAGGCGATCCGCGCCGGCATCGAGGGGCACAAGCGGGTTCCCGGCCGCCTGGAGCGGGTGGAAACGGAAGAAGGGATCAC
CGTCCTCGTCGACTACGCCCATACCGGGGACGCCCTGGAGAACGTCCTTTCCACCGTCTCCGAACTGAAGACGGACCGGA
TCATCACCATCTTCGGCTGCGGCGGCGATCGCGACAAAGGGAAACGCCCGGTGATGGGGGAGATAGCCGCCCGCTACAGC
GACCTTGCCGTCATCACCTCGGACAACCCCCGCACCGAGGACCCGCAGGCGATCCTGGAGGATGTCCGCGCCGGGATCCT
GCCGCTCGGCCTGCGCGAGTACGGCCTGGAGGAACTCGCCGCAGGGTTCCACGAGAAGGGGTTCGCCACCATCGAGTCGC
GCCGTGCCGCCATCCGGGCCGCGCTTTTGGCCGCGCACCCCGGAGACATCGTGCTTCTGGCCGGCAAGGGGCACGAGGAC
TACCAGATCGTCGGGACCGAGAAGTTCCACTTCGACGACAGGGAAGAGGCTGCGGCCGCGCTGAAACTAAGAGGTTAG

Upstream 100 bases:

>100_bases
GGCAGGGCGGTCGAGCAGAACCCGGCCCCCGGGAGCCGCATCACCACGCAGGATCAGGTCTGGGTCAAGTTCGTCCCTTC
GGCCTGACAATGGGGGTAGC

Downstream 100 bases:

>100_bases
TAATTGGGGAAAATTAGGTTCCCTTTTCCGCGTTTTTGCGGCACATTACGTGGTTTCGGTCCTGGACCGGGGCAAGTAAA
CAGGGCGCGTCGCGCGCCGG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MKLAQLLNCLASPELSGDAGIEIESLCYDSRKVAPGALFFALRGVKSDGTEFVEAAVKGGAVAIVADRPCACPGVTVVVV
PDPRLAMSLMAAAFYGTPTRGIPVVGITGTNGKTTTTYLVEGIMEQAGVPAAVLGTISYRFGETNIPAPNTTPESVDLQA
ILRELVDHGARGAVMEVSSHALEQHRVDGCLFDVALFSNLTRDHLDYHLDMESYFKSKLRLFTDLLAPTAEKPLRRAVVN
LDDPFGPRIAEKAAAPVLTYSMTGKADISVAEAEFSVDGIRCRLNTPVGEISLASELLGRFNLYNILSAVGAGLALGYSK
EAIRAGIEGHKRVPGRLERVETEEGITVLVDYAHTGDALENVLSTVSELKTDRIITIFGCGGDRDKGKRPVMGEIAARYS
DLAVITSDNPRTEDPQAILEDVRAGILPLGLREYGLEELAAGFHEKGFATIESRRAAIRAALLAAHPGDIVLLAGKGHED
YQIVGTEKFHFDDREEAAAALKLRG

Sequences:

>Translated_505_residues
MKLAQLLNCLASPELSGDAGIEIESLCYDSRKVAPGALFFALRGVKSDGTEFVEAAVKGGAVAIVADRPCACPGVTVVVV
PDPRLAMSLMAAAFYGTPTRGIPVVGITGTNGKTTTTYLVEGIMEQAGVPAAVLGTISYRFGETNIPAPNTTPESVDLQA
ILRELVDHGARGAVMEVSSHALEQHRVDGCLFDVALFSNLTRDHLDYHLDMESYFKSKLRLFTDLLAPTAEKPLRRAVVN
LDDPFGPRIAEKAAAPVLTYSMTGKADISVAEAEFSVDGIRCRLNTPVGEISLASELLGRFNLYNILSAVGAGLALGYSK
EAIRAGIEGHKRVPGRLERVETEEGITVLVDYAHTGDALENVLSTVSELKTDRIITIFGCGGDRDKGKRPVMGEIAARYS
DLAVITSDNPRTEDPQAILEDVRAGILPLGLREYGLEELAAGFHEKGFATIESRRAAIRAALLAAHPGDIVLLAGKGHED
YQIVGTEKFHFDDREEAAAALKLRG
>Mature_505_residues
MKLAQLLNCLASPELSGDAGIEIESLCYDSRKVAPGALFFALRGVKSDGTEFVEAAVKGGAVAIVADRPCACPGVTVVVV
PDPRLAMSLMAAAFYGTPTRGIPVVGITGTNGKTTTTYLVEGIMEQAGVPAAVLGTISYRFGETNIPAPNTTPESVDLQA
ILRELVDHGARGAVMEVSSHALEQHRVDGCLFDVALFSNLTRDHLDYHLDMESYFKSKLRLFTDLLAPTAEKPLRRAVVN
LDDPFGPRIAEKAAAPVLTYSMTGKADISVAEAEFSVDGIRCRLNTPVGEISLASELLGRFNLYNILSAVGAGLALGYSK
EAIRAGIEGHKRVPGRLERVETEEGITVLVDYAHTGDALENVLSTVSELKTDRIITIFGCGGDRDKGKRPVMGEIAARYS
DLAVITSDNPRTEDPQAILEDVRAGILPLGLREYGLEELAAGFHEKGFATIESRRAAIRAALLAAHPGDIVLLAGKGHED
YQIVGTEKFHFDDREEAAAALKLRG

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=487, Percent_Identity=38.6036960985626, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI1786274, Length=397, Percent_Identity=26.448362720403, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53954; Mature: 53954

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAQLLNCLASPELSGDAGIEIESLCYDSRKVAPGALFFALRGVKSDGTEFVEAAVKGG
CCHHHHHHHHCCCCCCCCCCCEEHHHHCCCCCCCCHHEEHHHHCCCCCCHHHHHHHHCCC
AVAIVADRPCACPGVTVVVVPDPRLAMSLMAAAFYGTPTRGIPVVGITGTNGKTTTTYLV
EEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHH
EGIMEQAGVPAAVLGTISYRFGETNIPAPNTTPESVDLQAILRELVDHGARGAVMEVSSH
HHHHHHCCCCEEHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEHHHHHH
ALEQHRVDGCLFDVALFSNLTRDHLDYHLDMESYFKSKLRLFTDLLAPTAEKPLRRAVVN
HHHHHHHHHHHHHHHHHHHCCHHHHHEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
LDDPFGPRIAEKAAAPVLTYSMTGKADISVAEAEFSVDGIRCRLNTPVGEISLASELLGR
CCCCCCCHHHHHHCCCEEEEEECCCCCEEEEEEEECCCCEEEEECCCCCHHHHHHHHHHH
FNLYNILSAVGAGLALGYSKEAIRAGIEGHKRVPGRLERVETEEGITVLVDYAHTGDALE
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCHHHH
NVLSTVSELKTDRIITIFGCGGDRDKGKRPVMGEIAARYSDLAVITSDNPRTEDPQAILE
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCHHHHHH
DVRAGILPLGLREYGLEELAAGFHEKGFATIESRRAAIRAALLAAHPGDIVLLAGKGHED
HHHHCCCCCCHHHCCHHHHHHCHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
YQIVGTEKFHFDDREEAAAALKLRG
EEEEECEEECCCCHHHHHHHHCCCC
>Mature Secondary Structure
MKLAQLLNCLASPELSGDAGIEIESLCYDSRKVAPGALFFALRGVKSDGTEFVEAAVKGG
CCHHHHHHHHCCCCCCCCCCCEEHHHHCCCCCCCCHHEEHHHHCCCCCCHHHHHHHHCCC
AVAIVADRPCACPGVTVVVVPDPRLAMSLMAAAFYGTPTRGIPVVGITGTNGKTTTTYLV
EEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHH
EGIMEQAGVPAAVLGTISYRFGETNIPAPNTTPESVDLQAILRELVDHGARGAVMEVSSH
HHHHHHCCCCEEHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEHHHHHH
ALEQHRVDGCLFDVALFSNLTRDHLDYHLDMESYFKSKLRLFTDLLAPTAEKPLRRAVVN
HHHHHHHHHHHHHHHHHHHCCHHHHHEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
LDDPFGPRIAEKAAAPVLTYSMTGKADISVAEAEFSVDGIRCRLNTPVGEISLASELLGR
CCCCCCCHHHHHHCCCEEEEEECCCCCEEEEEEEECCCCEEEEECCCCCHHHHHHHHHHH
FNLYNILSAVGAGLALGYSKEAIRAGIEGHKRVPGRLERVETEEGITVLVDYAHTGDALE
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCHHHH
NVLSTVSELKTDRIITIFGCGGDRDKGKRPVMGEIAARYSDLAVITSDNPRTEDPQAILE
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCHHHHHH
DVRAGILPLGLREYGLEELAAGFHEKGFATIESRRAAIRAALLAAHPGDIVLLAGKGHED
HHHHCCCCCCHHHCCHHHHHHCHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
YQIVGTEKFHFDDREEAAAALKLRG
EEEEECEEECCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA