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The map label for this gene is hyaP [H]

Identifier: 197116778

GI number: 197116778

Start: 456729

End: 457259

Strand: Reverse

Name: hyaP [H]

Synonym: Gbem_0380

Alternate gene names: 197116778

Gene position: 457259-456729 (Counterclockwise)

Preceding gene: 197116780

Following gene: 197116777

Centisome position: 9.91

GC content: 59.51

Gene sequence:

>531_bases
ATGCCTTTACGCCAAAATTCATCGCCGAGAATTCTAGTTCTCGGCATCGGCAATACCATAATGAGCGACGACGGGGTGGG
CGCAAAGGTCGTTCAGGAACTGCAGCGGGAGTTCCATTTCCCCAGTGGCGTCGACGTCGTCGACGGGGGGACCATGGGCC
TCGACTTGCTGCCGCTTTTGGAAGGGAGAAGCCATGTCATCATGGTAGACGCGGTCGAAACCGGCAAGCGGCCAGGTTCC
TGCGTGAGGTTGACCGGCGAGGAACTCCCCTCCGCTTTGGAAGCGAAGCTCTCGCCGCACCAGATCGGGCTGAAGGACCT
GCTGGGCGTCGCCCAACTGACGGGGCACGCCATCCCGGAACTCGTTCTCATAGGCGTACAGCCCAAGTCCATTGCTGTGG
GTTCTGAGCTTACCGCAGAGGTCAACCTGCAGATGGAGACGATGAAAGGGGCGGTCCTAAAAGAGTTGGAGAGGATCGGC
GCCCACGTCAAGCCTCTGGCGAAATCCTCTCCCTTCCGATGGGACCAGTAA

Upstream 100 bases:

>100_bases
GAGTGGAGTAACGATGGATAGCTATACGCGGTGAAGCCGGCAGCCTATCCTGTAAACTAGGCTGGAGCAGCGATTGGAGA
GGGTTCGACAGGGGGGCAAG

Downstream 100 bases:

>100_bases
ACGCCAACGCCTCCGGCAAGTCGACTGTTCGCCGGGGCCTTCCCACCTTACCCCCCGCCGTTCCACAAGCCTCCCGCCTG
CCAGGGCGAGGGGCTTTTTC

Product: [NiFe]-hydrogenase maturation protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MPLRQNSSPRILVLGIGNTIMSDDGVGAKVVQELQREFHFPSGVDVVDGGTMGLDLLPLLEGRSHVIMVDAVETGKRPGS
CVRLTGEELPSALEAKLSPHQIGLKDLLGVAQLTGHAIPELVLIGVQPKSIAVGSELTAEVNLQMETMKGAVLKELERIG
AHVKPLAKSSPFRWDQ

Sequences:

>Translated_176_residues
MPLRQNSSPRILVLGIGNTIMSDDGVGAKVVQELQREFHFPSGVDVVDGGTMGLDLLPLLEGRSHVIMVDAVETGKRPGS
CVRLTGEELPSALEAKLSPHQIGLKDLLGVAQLTGHAIPELVLIGVQPKSIAVGSELTAEVNLQMETMKGAVLKELERIG
AHVKPLAKSSPFRWDQ
>Mature_175_residues
PLRQNSSPRILVLGIGNTIMSDDGVGAKVVQELQREFHFPSGVDVVDGGTMGLDLLPLLEGRSHVIMVDAVETGKRPGSC
VRLTGEELPSALEAKLSPHQIGLKDLLGVAQLTGHAIPELVLIGVQPKSIAVGSELTAEVNLQMETMKGAVLKELERIGA
HVKPLAKSSPFRWDQ

Specific function: Protease involved in the C-terminal processing of hybC, the large subunit of hydrogenase 2. Specifically cleaves off a 15 amino acid peptide from the C-terminus of the precursor of hybC [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789367, Length=157, Percent_Identity=37.5796178343949, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1787209, Length=123, Percent_Identity=39.0243902439024, Blast_Score=106, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 18797; Mature: 18666

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLRQNSSPRILVLGIGNTIMSDDGVGAKVVQELQREFHFPSGVDVVDGGTMGLDLLPLL
CCCCCCCCCEEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCEEHHHHH
EGRSHVIMVDAVETGKRPGSCVRLTGEELPSALEAKLSPHQIGLKDLLGVAQLTGHAIPE
CCCCEEEEEEECCCCCCCCCEEEECHHHCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCE
LVLIGVQPKSIAVGSELTAEVNLQMETMKGAVLKELERIGAHVKPLAKSSPFRWDQ
EEEEECCCCCEEECCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PLRQNSSPRILVLGIGNTIMSDDGVGAKVVQELQREFHFPSGVDVVDGGTMGLDLLPLL
CCCCCCCCEEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCEEHHHHH
EGRSHVIMVDAVETGKRPGSCVRLTGEELPSALEAKLSPHQIGLKDLLGVAQLTGHAIPE
CCCCEEEEEEECCCCCCCCCEEEECHHHCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCE
LVLIGVQPKSIAVGSELTAEVNLQMETMKGAVLKELERIGAHVKPLAKSSPFRWDQ
EEEEECCCCCEEECCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8021226; 9278503; 10331925 [H]