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The map label for this gene is gcvH [H]

Identifier: 194736611

GI number: 194736611

Start: 3117708

End: 3118097

Strand: Reverse

Name: gcvH [H]

Synonym: SeSA_A3224

Alternate gene names: 194736611

Gene position: 3118097-3117708 (Counterclockwise)

Preceding gene: 194735702

Following gene: 194734851

Centisome position: 66.21

GC content: 55.13

Gene sequence:

>390_bases
ATGAGCAACGTACCCGCAGAACTGAAATACAGCAAAGAACACGAATGGCTGCGTAAAGAAGCGGATGGCACTTACACCGT
TGGCATTACTGAACACGCTCAGGAGCTGTTGGGCGATATGGTTTTTGTTGATCTGCCGGAAGTCGGCGCGACCGTAAGCG
CGGGCGACGACTGCGCCGTGGCGGAGTCCGTAAAAGCGGCGTCCGACATTTATGCGCCGGTAAGCGGCGAGATCGTGGCG
GTAAACGATGCGCTGAGCGACTCCCCGGAGCTGGTGAACAGCGAACCGTATGCCGGTGGCTGGATCTTTAAAATCAAAGC
CAGCGATGAAAGCGAACTTGAATCGCTGCTGGATGCCACCGCGTATGAGGCATTGTTAGAAGACGAATAA

Upstream 100 bases:

>100_bases
CAAATTCGTAACCGTGAAATGCCGGTAAAAGTGACTAAACCTGTTTTTGTGCGTAACGGCAAAGCCGTCGCGTGATTCAT
CCTTTTTTGGAGATTAATTG

Downstream 100 bases:

>100_bases
TGTTGTGCCGGATGGCGCTAATGCTTATCCGGCCTACTCACTGCAACGCGAATGATTTTGCGTATTCGTTTTCGCACGCG
TAGGCCCGGTAAGCGTCAGC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVA
VNDALSDSPELVNSEPYAGGWIFKIKASDESELESLLDATAYEALLEDE

Sequences:

>Translated_129_residues
MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVA
VNDALSDSPELVNSEPYAGGWIFKIKASDESELESLLDATAYEALLEDE
>Mature_128_residues
SNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAV
NDALSDSPELVNSEPYAGGWIFKIKASDESELESLLDATAYEALLEDE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=45, Blast_Score=113, Evalue=5e-26,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=44.1666666666667, Blast_Score=110, Evalue=5e-25,
Organism=Escherichia coli, GI1789271, Length=129, Percent_Identity=100, Blast_Score=257, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17507493, Length=120, Percent_Identity=46.6666666666667, Blast_Score=115, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17551294, Length=121, Percent_Identity=43.801652892562, Blast_Score=108, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=128, Percent_Identity=38.28125, Blast_Score=100, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=44.1666666666667, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13811; Mature: 13680

Theoretical pI: Translated: 3.72; Mature: 3.72

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAV
CCCCCCCCEECHHHHHHHHCCCCCEEEEHHHHHHHHHHCEEEEECCCCCCEECCCCCCHH
AESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWIFKIKASDESELESLLDAT
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHH
AYEALLEDE
HHHHHHCCC
>Mature Secondary Structure 
SNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAV
CCCCCCCEECHHHHHHHHCCCCCEEEEHHHHHHHHHHCEEEEECCCCCCEECCCCCCHH
AESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWIFKIKASDESELESLLDAT
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHH
AYEALLEDE
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA