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The map label for this gene is kefF [H]

Identifier: 194735745

GI number: 194735745

Start: 98402

End: 98932

Strand: Direct

Name: kefF [H]

Synonym: SeSA_A0095

Alternate gene names: 194735745

Gene position: 98402-98932 (Clockwise)

Preceding gene: 194735205

Following gene: 194737490

Centisome position: 2.09

GC content: 53.11

Gene sequence:

>531_bases
ATGATCCTCATTATTTATGCGCACCCGTATCCGCATCATTCTCATGCGAATAAGCGGATGCTTGAACAGGCAGGGACGCT
GGAAAACGTCGAAATACGTTCGCTTTATCACCTCTATCCCGATTTCAATATTGACGTTGCCGCCGAGCAGGAGGTGCTGT
CGCGCGCAAGCCTTATCGTCTGGCAGCATCCGATGCAGTGGTACAGCGTCCCTCCTTTACTCAAATTATGGATGGATAAA
GTCCTCACCCACGGCTGGGCTTACGGTCATGGCGGTACGGCATTACACGGTAAACATCTGCTGTGGGCGGTAACGACCGG
CGGTGGTGAAAACCACTTTGCTATCGGTTCACATCCGGGATTTGACGTCCTTTCTCAGCCGCTACAGGCGACGGCGCTCT
ATTGCGGCCTTAAATGGCTGCCGCCGTTTGCCATGCACTGCACGTTCATTTGCGATGACGACACCTTACAGGCGCAGGCG
CGTCAGTATAAACAGCGTTTACTGACATGGCAGGAGGTGAATCATGGATAG

Upstream 100 bases:

>100_bases
ATTCAATTTTTTCTGATTAGCCGCTCAATAATATTCACTATTAGCCTGGCGAATTTGTCACTACACTTTGTGATAAAAAA
CAGATCATCACAGGCTAATC

Downstream 100 bases:

>100_bases
CCATACTCTACTGCAGGCGCTGATCTATCTTGGTTCGGCGGCGTTGATTGTGCCGATTGCGGTACGTTTGGGCTTAGGAT
CGGTGCTGGGGTATCTGATT

Product: glutathione-regulated potassium-efflux system ancillary protein KefF

Products: NAD(P); reduced acceptor [C]

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MILIIYAHPYPHHSHANKRMLEQAGTLENVEIRSLYHLYPDFNIDVAAEQEVLSRASLIVWQHPMQWYSVPPLLKLWMDK
VLTHGWAYGHGGTALHGKHLLWAVTTGGGENHFAIGSHPGFDVLSQPLQATALYCGLKWLPPFAMHCTFICDDDTLQAQA
RQYKQRLLTWQEVNHG

Sequences:

>Translated_176_residues
MILIIYAHPYPHHSHANKRMLEQAGTLENVEIRSLYHLYPDFNIDVAAEQEVLSRASLIVWQHPMQWYSVPPLLKLWMDK
VLTHGWAYGHGGTALHGKHLLWAVTTGGGENHFAIGSHPGFDVLSQPLQATALYCGLKWLPPFAMHCTFICDDDTLQAQA
RQYKQRLLTWQEVNHG
>Mature_176_residues
MILIIYAHPYPHHSHANKRMLEQAGTLENVEIRSLYHLYPDFNIDVAAEQEVLSRASLIVWQHPMQWYSVPPLLKLWMDK
VLTHGWAYGHGGTALHGKHLLWAVTTGGGENHFAIGSHPGFDVLSQPLQATALYCGLKWLPPFAMHCTFICDDDTLQAQA
RQYKQRLLTWQEVNHG

Specific function: Required for full activity of kefC [H]

COG id: COG2249

COG function: function code R; Putative NADPH-quinone reductase (modulator of drug activity B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily [H]

Homologues:

Organism=Homo sapiens, GI156564357, Length=162, Percent_Identity=32.7160493827161, Blast_Score=74, Evalue=6e-14,
Organism=Homo sapiens, GI4505415, Length=112, Percent_Identity=33.9285714285714, Blast_Score=68, Evalue=5e-12,
Organism=Escherichia coli, GI1786231, Length=176, Percent_Identity=87.5, Blast_Score=331, Evalue=1e-92,
Organism=Escherichia coli, GI1789750, Length=165, Percent_Identity=40, Blast_Score=117, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 1.6.99.- [C]

Molecular weight: Translated: 20073; Mature: 20073

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILIIYAHPYPHHSHANKRMLEQAGTLENVEIRSLYHLYPDFNIDVAAEQEVLSRASLIV
CEEEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHEEECCCCCEEECHHHHHHHHHHEEE
WQHPMQWYSVPPLLKLWMDKVLTHGWAYGHGGTALHGKHLLWAVTTGGGENHFAIGSHPG
EECCHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCEEEEEEEECCCCCEEEECCCCC
FDVLSQPLQATALYCGLKWLPPFAMHCTFICDDDTLQAQARQYKQRLLTWQEVNHG
HHHHHHHHHHHHHHHCCCCCCCHHHEEEEEECCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MILIIYAHPYPHHSHANKRMLEQAGTLENVEIRSLYHLYPDFNIDVAAEQEVLSRASLIV
CEEEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHEEECCCCCEEECHHHHHHHHHHEEE
WQHPMQWYSVPPLLKLWMDKVLTHGWAYGHGGTALHGKHLLWAVTTGGGENHFAIGSHPG
EECCHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCEEEEEEEECCCCCEEEECCCCC
FDVLSQPLQATALYCGLKWLPPFAMHCTFICDDDTLQAQARQYKQRLLTWQEVNHG
HHHHHHHHHHHHHHHCCCCCCCHHHEEEEEECCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; acceptor [C]

Specific reaction: NAD(P)H2 + acceptor = NAD(P) + reduced acceptor [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA