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The map label for this gene is ald [H]

Identifier: 194333595

GI number: 194333595

Start: 810861

End: 812081

Strand: Reverse

Name: ald [H]

Synonym: Paes_0761

Alternate gene names: 194333595

Gene position: 812081-810861 (Counterclockwise)

Preceding gene: 194333596

Following gene: 194333593

Centisome position: 32.32

GC content: 49.8

Gene sequence:

>1221_bases
ATGGGATACTCTGGAGACATTACAGGCATTGAGTTTGATCTTGGCATAAAGACCCTTGAGCGATGGCTTATCAAGCCGGA
AAAAACCATGAATATCGCTCTGGGAATTCCGAAAGAGCGGCTCAACGATGAGAGAAGAGTCGCCATTTCCCCTGCCGGGG
CCAGAATTCTGGTTGAAAACGGCATGAGGGTCATGATTGAACACGATGCCGGAAAAGCCAGCAATCTCTCCGACGAAGAC
TATAGTGATGCCGGGTGCACCATTACAGACTCGCTCGAAAAGCTTTACGATGAATCAAATATCATCGTAAAAGTCTCTCC
GCCCCAAAACCATGAGCTGCCGTTGTTCAAACCCTCCCAGATGCTCATTTCAGCTCTTCACCTTGGAGCCATCCACAGGC
CGATGATGAAGATGTTTCTGGAAAAAAACATCACCGCACTGGGGTTCGAATTTATTCAGACGCGCGATGGGGATCTCCCC
ATAGTCAGAACCCTGAGCGAAATAGCCGGCTCACTTGCAATTCAGACCGCAGCAAAATATCTGGAAACAGGTTACGGAGG
CAGCGGCATTCTGCTTGGAGGCATTGCCGGCGTTCCTCCTGCCCGAATCACCATTATCGGAGCAGGAACGGTTGGGCTGT
TTGCGGCACAGGATGCGCTTGGCCTCGGAGCGCAGGTCACGGTCATCGACAAAGAAATAAACCGCTTACGACGATTTGAG
GCGTTCTTTAACCGCCATATCGTTACCGCAATAACCAATGACCATTATATCGCTGAACTGGCCAAGGAATCCGATGTCAT
GATCGGTGCTCTCAGCCCGAAACAGAAATTCATCAAACCTATCGTCAGTGAAGAGGTTATCAAAAGCATGCGCCAGGGTT
CAGTCATTATTGATGTCTCAATAGATCAGGGCTCATGTTTTGAAACAAGTCGCCACACAACCCACACCAACCCAATTTTC
GTCAAGCACGGCGTCACGCACTACTGCGTTCCCAATATTCCTTCTGCCGTCGCTAAAACAGCGACCTTTGCCATGACCAA
CACCCTGCTCCCGTTTCTGATGAAACTCAACGCCCATGAAACCATCACGGATATTCTCTGGAACAGCCACAGCCTGAGAA
AAGGGACTTACGCATTCAAAGGCTATATCACCCAGAAAAACCTTGCTGAACTGACCGCTCTGCCATACAGGGAGATCGAT
ATGCTCCTGGCTACAGGGTAA

Upstream 100 bases:

>100_bases
CACAGTTGAAAAAAGCGGCAATACGAATGTAAACACGCATTGTGAGGTAACATCAAACCACATGAACGGTCACCAAACCT
CTTTCTACCTCTAAAGCGCG

Downstream 100 bases:

>100_bases
CAATTTCCTTCAGAGATACTCCAGAGGATTGCCAAGACTCCGTGCAATGTATGACGCACATGACACCCCTGAATAGGTCA
CAGCAATAACCCCCTGACCG

Product: alanine dehydrogenase/PNT domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MGYSGDITGIEFDLGIKTLERWLIKPEKTMNIALGIPKERLNDERRVAISPAGARILVENGMRVMIEHDAGKASNLSDED
YSDAGCTITDSLEKLYDESNIIVKVSPPQNHELPLFKPSQMLISALHLGAIHRPMMKMFLEKNITALGFEFIQTRDGDLP
IVRTLSEIAGSLAIQTAAKYLETGYGGSGILLGGIAGVPPARITIIGAGTVGLFAAQDALGLGAQVTVIDKEINRLRRFE
AFFNRHIVTAITNDHYIAELAKESDVMIGALSPKQKFIKPIVSEEVIKSMRQGSVIIDVSIDQGSCFETSRHTTHTNPIF
VKHGVTHYCVPNIPSAVAKTATFAMTNTLLPFLMKLNAHETITDILWNSHSLRKGTYAFKGYITQKNLAELTALPYREID
MLLATG

Sequences:

>Translated_406_residues
MGYSGDITGIEFDLGIKTLERWLIKPEKTMNIALGIPKERLNDERRVAISPAGARILVENGMRVMIEHDAGKASNLSDED
YSDAGCTITDSLEKLYDESNIIVKVSPPQNHELPLFKPSQMLISALHLGAIHRPMMKMFLEKNITALGFEFIQTRDGDLP
IVRTLSEIAGSLAIQTAAKYLETGYGGSGILLGGIAGVPPARITIIGAGTVGLFAAQDALGLGAQVTVIDKEINRLRRFE
AFFNRHIVTAITNDHYIAELAKESDVMIGALSPKQKFIKPIVSEEVIKSMRQGSVIIDVSIDQGSCFETSRHTTHTNPIF
VKHGVTHYCVPNIPSAVAKTATFAMTNTLLPFLMKLNAHETITDILWNSHSLRKGTYAFKGYITQKNLAELTALPYREID
MLLATG
>Mature_405_residues
GYSGDITGIEFDLGIKTLERWLIKPEKTMNIALGIPKERLNDERRVAISPAGARILVENGMRVMIEHDAGKASNLSDEDY
SDAGCTITDSLEKLYDESNIIVKVSPPQNHELPLFKPSQMLISALHLGAIHRPMMKMFLEKNITALGFEFIQTRDGDLPI
VRTLSEIAGSLAIQTAAKYLETGYGGSGILLGGIAGVPPARITIIGAGTVGLFAAQDALGLGAQVTVIDKEINRLRRFEA
FFNRHIVTAITNDHYIAELAKESDVMIGALSPKQKFIKPIVSEEVIKSMRQGSVIIDVSIDQGSCFETSRHTTHTNPIFV
KHGVTHYCVPNIPSAVAKTATFAMTNTLLPFLMKLNAHETITDILWNSHSLRKGTYAFKGYITQKNLAELTALPYREIDM
LLATG

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=370, Percent_Identity=29.1891891891892, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI122939153, Length=370, Percent_Identity=29.1891891891892, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1787887, Length=298, Percent_Identity=29.5302013422819, Blast_Score=105, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17550456, Length=355, Percent_Identity=27.887323943662, Blast_Score=131, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 44505; Mature: 44374

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYSGDITGIEFDLGIKTLERWLIKPEKTMNIALGIPKERLNDERRVAISPAGARILVEN
CCCCCCCCEEEEECCHHHHHHHHCCCCCCEEEEECCCHHHCCCCCEEEECCCCCEEEEEC
GMRVMIEHDAGKASNLSDEDYSDAGCTITDSLEKLYDESNIIVKVSPPQNHELPLFKPSQ
CCEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHH
MLISALHLGAIHRPMMKMFLEKNITALGFEFIQTRDGDLPIVRTLSEIAGSLAIQTAAKY
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
LETGYGGSGILLGGIAGVPPARITIIGAGTVGLFAAQDALGLGAQVTVIDKEINRLRRFE
HHCCCCCCEEEEECCCCCCCCEEEEEECCCHHEEEHHHHHCCCCEEEEEHHHHHHHHHHH
AFFNRHIVTAITNDHYIAELAKESDVMIGALSPKQKFIKPIVSEEVIKSMRQGSVIIDVS
HHHCCEEEEEEECCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEE
IDQGSCFETSRHTTHTNPIFVKHGVTHYCVPNIPSAVAKTATFAMTNTLLPFLMKLNAHE
ECCCCCCCCCCCCCCCCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
TITDILWNSHSLRKGTYAFKGYITQKNLAELTALPYREIDMLLATG
HHHHHHCCCCCCCCCCEEEEEEEECCHHHHHHCCCHHHHHEEEECC
>Mature Secondary Structure 
GYSGDITGIEFDLGIKTLERWLIKPEKTMNIALGIPKERLNDERRVAISPAGARILVEN
CCCCCCCEEEEECCHHHHHHHHCCCCCCEEEEECCCHHHCCCCCEEEECCCCCEEEEEC
GMRVMIEHDAGKASNLSDEDYSDAGCTITDSLEKLYDESNIIVKVSPPQNHELPLFKPSQ
CCEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHH
MLISALHLGAIHRPMMKMFLEKNITALGFEFIQTRDGDLPIVRTLSEIAGSLAIQTAAKY
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
LETGYGGSGILLGGIAGVPPARITIIGAGTVGLFAAQDALGLGAQVTVIDKEINRLRRFE
HHCCCCCCEEEEECCCCCCCCEEEEEECCCHHEEEHHHHHCCCCEEEEEHHHHHHHHHHH
AFFNRHIVTAITNDHYIAELAKESDVMIGALSPKQKFIKPIVSEEVIKSMRQGSVIIDVS
HHHCCEEEEEEECCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEE
IDQGSCFETSRHTTHTNPIFVKHGVTHYCVPNIPSAVAKTATFAMTNTLLPFLMKLNAHE
ECCCCCCCCCCCCCCCCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
TITDILWNSHSLRKGTYAFKGYITQKNLAELTALPYREIDMLLATG
HHHHHHCCCCCCCCCCEEEEEEEECCHHHHHHCCCHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2340274 [H]