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The map label for this gene is rpoE [H]

Identifier: 194333593

GI number: 194333593

Start: 808479

End: 809108

Strand: Reverse

Name: rpoE [H]

Synonym: Paes_0759

Alternate gene names: 194333593

Gene position: 809108-808479 (Counterclockwise)

Preceding gene: 194333595

Following gene: 194333592

Centisome position: 32.2

GC content: 41.43

Gene sequence:

>630_bases
ATGATCAACTCTTCCTCCTCACATAAACCTCCCGCTCTGCTAAAAAAAGAGCACGTGTCGGCTCAGGAACAGCAGAAACA
AATCGAGTTTCAGCAGGAAGCCATACAGCACATTAATGCATTATATAATTATGCATTACACCTGACAATGAATCCTGATG
ATGCCAAGGATCTCGTTCAGGAAACCTATCTCAAAGCATATAAATTCTTTGGCTCGTTTGAAAAGGGAACCAACTGCAAG
GCGTGGCTGTTTAAAATCCTTAAAAACAATTACATCAACAAATTCAGGAAAAACTCAAGAGAACCCGGAAAGGTAGACTA
TGACCTGATCAAGGATTTTTATCATTCAATCAAAGCAGCGCAAAGCGATAACGAAGATCTCAATGCGGGATATTTTAAAT
CGCTCATGCATGATGAAGTCTACCAGGCTCTGCAATCTCTCCCTGACGAGTTCAAAGAGGTGATACAATTATGCGATATC
GAAGGGTTCACCTATGAAGAGATTGCCAACATGGTGGAAAGCCCCATCGGTACAGTTCGTTCACGCCTTTACAGAGGCAG
AAAGCTTTTACGTTCCAAACTGAATGATTTTGCCAAAAAATACGGTTACCACACCGAAGACAACGAATAA

Upstream 100 bases:

>100_bases
AAACGATCAACTGAAGTAAAAATATATTTTTTATTTTAATAGGTAAGAATCTCTATCAGCTCCAGCATTTAAATATCCGT
CTTCGTAACCTTACCAGACT

Downstream 100 bases:

>100_bases
TTAATTTTCTTAACACCTCAGTATTATGACAACTGTGAATTGTAAAGAAGCCATGGCGCTCATGAGTGCCGCAGTTGATG
GAGAACTTTGCAGCAGGGAG

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MINSSSSHKPPALLKKEHVSAQEQQKQIEFQQEAIQHINALYNYALHLTMNPDDAKDLVQETYLKAYKFFGSFEKGTNCK
AWLFKILKNNYINKFRKNSREPGKVDYDLIKDFYHSIKAAQSDNEDLNAGYFKSLMHDEVYQALQSLPDEFKEVIQLCDI
EGFTYEEIANMVESPIGTVRSRLYRGRKLLRSKLNDFAKKYGYHTEDNE

Sequences:

>Translated_209_residues
MINSSSSHKPPALLKKEHVSAQEQQKQIEFQQEAIQHINALYNYALHLTMNPDDAKDLVQETYLKAYKFFGSFEKGTNCK
AWLFKILKNNYINKFRKNSREPGKVDYDLIKDFYHSIKAAQSDNEDLNAGYFKSLMHDEVYQALQSLPDEFKEVIQLCDI
EGFTYEEIANMVESPIGTVRSRLYRGRKLLRSKLNDFAKKYGYHTEDNE
>Mature_209_residues
MINSSSSHKPPALLKKEHVSAQEQQKQIEFQQEAIQHINALYNYALHLTMNPDDAKDLVQETYLKAYKFFGSFEKGTNCK
AWLFKILKNNYINKFRKNSREPGKVDYDLIKDFYHSIKAAQSDNEDLNAGYFKSLMHDEVYQALQSLPDEFKEVIQLCDI
EGFTYEEIANMVESPIGTVRSRLYRGRKLLRSKLNDFAKKYGYHTEDNE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=150, Percent_Identity=31.3333333333333, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 24437; Mature: 24437

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINSSSSHKPPALLKKEHVSAQEQQKQIEFQQEAIQHINALYNYALHLTMNPDDAKDLVQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHH
ETYLKAYKFFGSFEKGTNCKAWLFKILKNNYINKFRKNSREPGKVDYDLIKDFYHSIKAA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
QSDNEDLNAGYFKSLMHDEVYQALQSLPDEFKEVIQLCDIEGFTYEEIANMVESPIGTVR
CCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
SRLYRGRKLLRSKLNDFAKKYGYHTEDNE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MINSSSSHKPPALLKKEHVSAQEQQKQIEFQQEAIQHINALYNYALHLTMNPDDAKDLVQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHH
ETYLKAYKFFGSFEKGTNCKAWLFKILKNNYINKFRKNSREPGKVDYDLIKDFYHSIKAA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
QSDNEDLNAGYFKSLMHDEVYQALQSLPDEFKEVIQLCDIEGFTYEEIANMVESPIGTVR
CCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
SRLYRGRKLLRSKLNDFAKKYGYHTEDNE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]