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The map label for this gene is nusB

Identifier: 194333302

GI number: 194333302

Start: 491910

End: 492416

Strand: Reverse

Name: nusB

Synonym: Paes_0459

Alternate gene names: 194333302

Gene position: 492416-491910 (Counterclockwise)

Preceding gene: 194333303

Following gene: 194333301

Centisome position: 19.6

GC content: 43.0

Gene sequence:

>507_bases
ATGAAGGCTCATCGACGACACATACGCGAAAAAATTGTACAGGCGCTCTACACCCTTGACGTTCGCGATACCGATACCGC
AACGGCTGGAGACTGGCTGATCACACCGGAAATATCAAAGGATCCCAAGGCTATCAGGTTTTTCAAACAGCTGCTGGGAG
CTATTGTGGACAACAGGGAAGAGATCGATCAATATATCTCAAAGCATACCTTCAACTGGGATATGAGCCGGATCGCCATC
ATTGATAAAAACATACTTCGAATGGCCATGGCGGAAATTCTCTATTTCGAAGATATTCCTCCAAAAGTATCGATCAATGA
AGCTATCGAGATCGCAAAAAAATTCAACAGCACGGACAAAAGCAGTAAATTTGTCAACGGAATTCTCGACGCCGTTTATA
ACGACCTGAACAAATCAGGAAAAATAAATAAATGCGGCCGGGGGCTGATTGATCAATCAAGCAATCTGAAAAAAAATAAA
GAAAAGAGCCCGTCTGATGATCAATAG

Upstream 100 bases:

>100_bases
CTCCTGGAACACAAGCCTGATGCTCTTTTTGAAGATTTCAGCGATACAGAAAAGGTCATCAGAAGCATTGTCTCATTTTA
ATTAAATCAACTGCTCTTTT

Downstream 100 bases:

>100_bases
AAAAGAGGAGCTCTCCTTACTCCATGCCTGAATCGATTTCCCAGAACGCCAGAATCATTGCTGTCGGCGACATTCACGGA
TGTATCGCATCGCTGAAAAA

Product: NusB antitermination factor

Products: NA

Alternate protein names: Protein nusB

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MKAHRRHIREKIVQALYTLDVRDTDTATAGDWLITPEISKDPKAIRFFKQLLGAIVDNREEIDQYISKHTFNWDMSRIAI
IDKNILRMAMAEILYFEDIPPKVSINEAIEIAKKFNSTDKSSKFVNGILDAVYNDLNKSGKINKCGRGLIDQSSNLKKNK
EKSPSDDQ

Sequences:

>Translated_168_residues
MKAHRRHIREKIVQALYTLDVRDTDTATAGDWLITPEISKDPKAIRFFKQLLGAIVDNREEIDQYISKHTFNWDMSRIAI
IDKNILRMAMAEILYFEDIPPKVSINEAIEIAKKFNSTDKSSKFVNGILDAVYNDLNKSGKINKCGRGLIDQSSNLKKNK
EKSPSDDQ
>Mature_168_residues
MKAHRRHIREKIVQALYTLDVRDTDTATAGDWLITPEISKDPKAIRFFKQLLGAIVDNREEIDQYISKHTFNWDMSRIAI
IDKNILRMAMAEILYFEDIPPKVSINEAIEIAKKFNSTDKSSKFVNGILDAVYNDLNKSGKINKCGRGLIDQSSNLKKNK
EKSPSDDQ

Specific function: Involved in the transcription termination process

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family

Homologues:

Organism=Escherichia coli, GI1786618, Length=121, Percent_Identity=35.5371900826446, Blast_Score=70, Evalue=8e-14,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NUSB_PROA2 (B4S517)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002015162.1
- ProteinModelPortal:   B4S517
- SMR:   B4S517
- GeneID:   6460063
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_0459
- HOGENOM:   HBG734409
- OMA:   PDIPKNV
- ProtClustDB:   CLSK637901
- HAMAP:   MF_00073
- InterPro:   IPR011605
- InterPro:   IPR006027
- Gene3D:   G3DSA:1.10.940.10
- TIGRFAMs:   TIGR01951

Pfam domain/function: PF01029 NusB; SSF48013 NusB_RsmB_TIM44

EC number: NA

Molecular weight: Translated: 19242; Mature: 19242

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAHRRHIREKIVQALYTLDVRDTDTATAGDWLITPEISKDPKAIRFFKQLLGAIVDNRE
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCHH
EIDQYISKHTFNWDMSRIAIIDKNILRMAMAEILYFEDIPPKVSINEAIEIAKKFNSTDK
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCH
SSKFVNGILDAVYNDLNKSGKINKCGRGLIDQSSNLKKNKEKSPSDDQ
HHHHHHHHHHHHHHHHCCCCCCCHHHCCHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKAHRRHIREKIVQALYTLDVRDTDTATAGDWLITPEISKDPKAIRFFKQLLGAIVDNRE
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCHH
EIDQYISKHTFNWDMSRIAIIDKNILRMAMAEILYFEDIPPKVSINEAIEIAKKFNSTDK
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCH
SSKFVNGILDAVYNDLNKSGKINKCGRGLIDQSSNLKKNKEKSPSDDQ
HHHHHHHHHHHHHHHHCCCCCCCHHHCCHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA