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The map label for this gene is pphA [C]

Identifier: 194333301

GI number: 194333301

Start: 491194

End: 491886

Strand: Reverse

Name: pphA [C]

Synonym: Paes_0458

Alternate gene names: 194333301

Gene position: 491886-491194 (Counterclockwise)

Preceding gene: 194333302

Following gene: 194333300

Centisome position: 19.57

GC content: 49.21

Gene sequence:

>693_bases
ATGCCTGAATCGATTTCCCAGAACGCCAGAATCATTGCTGTCGGCGACATTCACGGATGTATCGCATCGCTGAAAAAGCT
CTGCCGGCAGCTTGAGCTGCAACCATCTGACCAGCTTGTATTTCTTGGAGACTATATCGACAGGGGGAAAAATGCTCAAG
CAGTGATCGACTTCCTGATAGACCTGCGGCGTTCGTTCACATGTTTTTTTCTCATGGGTAACCATGAACTGATGTTGCTC
GACTCCCTCAAAACCGGCAATGCCTCCGATTGGCTCCGCAATGGCGGTAAGGAGACACTCCAGTCATACGCAGTCAAGAG
CGCCAGGGATCTTCCCCTGAGCCATATCGGGTTTCTGCAGTCATGCCACTATTATCTTGAATCCAAGGATTTTATCTTCG
TTCACGGCGGCCTGGACCCGGATATGAGCATCAAAAACAATATCGGGTACCTCAAACCTGCGGATTTCTGCTGGATGAGA
ACCCACCTGCGTTCATCATATCTGGAGACAAACCGCTACAACTGGGAAAAAACCGTTGTATGCGCTCACACACCCGCTCC
GGAAATAATCAATCTTAAAAAGCTGATCGCCATCGATACAGGATGCGTTTACAAGGACGATCCGTCTCTGGGAAAGCTTT
CTGCCATAATACTCCCCTCCCGTCAACTGGTCCAGACAGTAAACATCGATTAA

Upstream 100 bases:

>100_bases
AATGCGGCCGGGGGCTGATTGATCAATCAAGCAATCTGAAAAAAAATAAAGAAAAGAGCCCGTCTGATGATCAATAGAAA
AGAGGAGCTCTCCTTACTCC

Downstream 100 bases:

>100_bases
ACCGACCATTATGAATGTTGCAGAAAAAATCGCCTTCATCAATGAAGCCCTGGGAAACAAATATCCCTCACCAAAAAGCG
AGCTGCAGTACCAGAGCCCT

Product: metallophosphoesterase

Products: A Protein; Orthophosphate. [C]

Alternate protein names: Serine/Threonine Protein Phosphatase; Bis(5Nucleosyl)-Tetraphosphatase ApaH; Serine/Threonine Protein Phosphatase Family Protein; Ser/Thr Protein Phosphatase Family Protein; Serine/Threonine Specific Protein Phosphatase Protein; Serine/Threonine Specific Protein Phosphatase; Serine/Threonine Protein Phosphatase I; Phosphoprotein Phosphatase; Calcineurin-Like Phosphoesterase; Metallophosphoesterase Domain-Containing Protein; Serine-Threonine Protein Phosphatase; Protein Serine-Threonine Phosphatase; Bis(5-Nucleosyl)-Tetraphosphatase; Diadenosine Tetraphosphatase; Protein Phosphatase; Diadenosine Tetraphosphatase-Like Protein; Serinethreonine Protein Phosphatase; Kinase; Serine/Threonine Protein Phosphatase PrpA; Serine/Threonine Protein Phosphatase Protein; Phosphoesterase

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MPESISQNARIIAVGDIHGCIASLKKLCRQLELQPSDQLVFLGDYIDRGKNAQAVIDFLIDLRRSFTCFFLMGNHELMLL
DSLKTGNASDWLRNGGKETLQSYAVKSARDLPLSHIGFLQSCHYYLESKDFIFVHGGLDPDMSIKNNIGYLKPADFCWMR
THLRSSYLETNRYNWEKTVVCAHTPAPEIINLKKLIAIDTGCVYKDDPSLGKLSAIILPSRQLVQTVNID

Sequences:

>Translated_230_residues
MPESISQNARIIAVGDIHGCIASLKKLCRQLELQPSDQLVFLGDYIDRGKNAQAVIDFLIDLRRSFTCFFLMGNHELMLL
DSLKTGNASDWLRNGGKETLQSYAVKSARDLPLSHIGFLQSCHYYLESKDFIFVHGGLDPDMSIKNNIGYLKPADFCWMR
THLRSSYLETNRYNWEKTVVCAHTPAPEIINLKKLIAIDTGCVYKDDPSLGKLSAIILPSRQLVQTVNID
>Mature_229_residues
PESISQNARIIAVGDIHGCIASLKKLCRQLELQPSDQLVFLGDYIDRGKNAQAVIDFLIDLRRSFTCFFLMGNHELMLLD
SLKTGNASDWLRNGGKETLQSYAVKSARDLPLSHIGFLQSCHYYLESKDFIFVHGGLDPDMSIKNNIGYLKPADFCWMRT
HLRSSYLETNRYNWEKTVVCAHTPAPEIINLKKLIAIDTGCVYKDDPSLGKLSAIILPSRQLVQTVNID

Specific function: Plays A Key Role In Signaling Protein Misfolding Via The Cpxr/Cpxa Transducing System. It Also Modulates The Phosphorylated Status Of Many Phosphoproteins In E. coli, Some Of Which Acting As Major Chaperones. Has Been Shown, In Vitro, To Act On Ser, Thr A

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.1.3.16 [C]

Molecular weight: Translated: 25952; Mature: 25821

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPESISQNARIIAVGDIHGCIASLKKLCRQLELQPSDQLVFLGDYIDRGKNAQAVIDFLI
CCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCEEEEECHHCCCCCHHHHHHHHH
DLRRSFTCFFLMGNHELMLLDSLKTGNASDWLRNGGKETLQSYAVKSARDLPLSHIGFLQ
HHHCCEEEEEEECCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHH
SCHYYLESKDFIFVHGGLDPDMSIKNNIGYLKPADFCWMRTHLRSSYLETNRYNWEKTVV
HHHHHHCCCCEEEEECCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
CAHTPAPEIINLKKLIAIDTGCVYKDDPSLGKLSAIILPSRQLVQTVNID
EECCCCCHHHCHHHHEEEECCCEECCCCCCCCEEEEEECCHHHHHEECCC
>Mature Secondary Structure 
PESISQNARIIAVGDIHGCIASLKKLCRQLELQPSDQLVFLGDYIDRGKNAQAVIDFLI
CCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCEEEEECHHCCCCCHHHHHHHHH
DLRRSFTCFFLMGNHELMLLDSLKTGNASDWLRNGGKETLQSYAVKSARDLPLSHIGFLQ
HHHCCEEEEEEECCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHH
SCHYYLESKDFIFVHGGLDPDMSIKNNIGYLKPADFCWMRTHLRSSYLETNRYNWEKTVV
HHHHHHCCCCEEEEECCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
CAHTPAPEIINLKKLIAIDTGCVYKDDPSLGKLSAIILPSRQLVQTVNID
EECCCCCHHHCHHHHEEEECCCEECCCCCCCCEEEEEECCHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe; Mn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Phosphoprotein; H2O [C]

Specific reaction: A Phosphoprotein + H2O = A Protein + Orthophosphate. [C]

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA