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The map label for this gene is thiL [H]

Identifier: 194333176

GI number: 194333176

Start: 360635

End: 361699

Strand: Reverse

Name: thiL [H]

Synonym: Paes_0332

Alternate gene names: 194333176

Gene position: 361699-360635 (Counterclockwise)

Preceding gene: 194333178

Following gene: 194333175

Centisome position: 14.39

GC content: 52.68

Gene sequence:

>1065_bases
ATGTCGTTGAAACCTATTGCTGATATTGGTGAATTTGGACTCATAGAAAAGATTGCCGCCATTTGCGCGCCAACCATCAA
ACCCGCTTCCGGAGTAGTGGAAGGAATAGGTGACGATTGCGCCGTCTATGAGCACTCCCCGTCATCAGTACAGGTAGCCA
CTACCGATATCCTCGCAGAACACCAGCACTTCGATCTGTTAACCACGCCGCCGCATCATCTGGGCAGCAAGGCCATCAGT
GTCAACGTCTCGGATATCTGCGCCATGAACGCCCTCCCCCGATACGCTCTCATTTCAATAGCGATGCCGCAGAACACCCC
GGTTGAAATGGTCGAATCACTCTATAACGGCATGGCACACGCAGCCAAACTCTACGGCACCGCTCTTGTCGGCGGCGATA
CCTCTTCGTCAGCAGCAGGCATCGTCATCTCCGTCACCCTCATCGGTGAGACCTCAAAAGAGAACCTCGCTCTACGCAGC
GGAGCCTCTCCGGGAGATCTTATCTGCGTCACAGGTGCCCTCGGGGGCGCCGCTGCAGGACTGAAGGTTCTCCTGCGCGA
AAAAAGGATCATGATGGACCATGTCCAGAACGGCGAACCATACAACAGAGACATCATGAACAACCTGGAAGAGTACACCG
AAGCCATTCAACATCAGCTTCTGCCATCGGCACGAGTCGATATCATCGAATTTTTCCACAAACACCAGATTGTCCCCACG
TCGATGATCGATATTTCTGACGGTCTGTCTTCCGACCTTCGCCACATCTGCCAGCAATCCATGGTCGGAGCCCGTATCGA
AGAAGGGCGCATCCCGATTCTTTCACAGGCACGCCATATCGCCGATGAATTCCAGGAAGATGCCCTTGACTATGCACTCG
GAGGAGGAGAAGACTACCAGCTGCTCTTCACCCTGAAACCCGACAACTTTCAGGCAATCGTCGAACATCGTGATATCACC
GTTATCGGAAAAATCACCCCGAAGGAAGAAGGCATGACGCTCAACGATATCTACGGCATGCAGATCGACATGAAAGAGAT
CAGAGGATTTGACCATTTCACATAA

Upstream 100 bases:

>100_bases
AAAAACAAATTATCTATGTTACATACATACAGGCAGCCTGCTTTTCCTGCGAGCAAAGCAGCAGGAGTTATTTCAGCCAT
GAACTTAAATTTACTGGAAA

Downstream 100 bases:

>100_bases
AAATGAGGCGACGTTCTTTTTTGCTTATTGTGTCCAAGATATTAAATTGATCGACCTGCCGAAGTGGCGGAACTGGTAGA
CGCCCAGGACTTAAAATCCT

Product: thiamine-monophosphate kinase

Products: NA

Alternate protein names: Thiamine-phosphate kinase [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSLKPIADIGEFGLIEKIAAICAPTIKPASGVVEGIGDDCAVYEHSPSSVQVATTDILAEHQHFDLLTTPPHHLGSKAIS
VNVSDICAMNALPRYALISIAMPQNTPVEMVESLYNGMAHAAKLYGTALVGGDTSSSAAGIVISVTLIGETSKENLALRS
GASPGDLICVTGALGGAAAGLKVLLREKRIMMDHVQNGEPYNRDIMNNLEEYTEAIQHQLLPSARVDIIEFFHKHQIVPT
SMIDISDGLSSDLRHICQQSMVGARIEEGRIPILSQARHIADEFQEDALDYALGGGEDYQLLFTLKPDNFQAIVEHRDIT
VIGKITPKEEGMTLNDIYGMQIDMKEIRGFDHFT

Sequences:

>Translated_354_residues
MSLKPIADIGEFGLIEKIAAICAPTIKPASGVVEGIGDDCAVYEHSPSSVQVATTDILAEHQHFDLLTTPPHHLGSKAIS
VNVSDICAMNALPRYALISIAMPQNTPVEMVESLYNGMAHAAKLYGTALVGGDTSSSAAGIVISVTLIGETSKENLALRS
GASPGDLICVTGALGGAAAGLKVLLREKRIMMDHVQNGEPYNRDIMNNLEEYTEAIQHQLLPSARVDIIEFFHKHQIVPT
SMIDISDGLSSDLRHICQQSMVGARIEEGRIPILSQARHIADEFQEDALDYALGGGEDYQLLFTLKPDNFQAIVEHRDIT
VIGKITPKEEGMTLNDIYGMQIDMKEIRGFDHFT
>Mature_353_residues
SLKPIADIGEFGLIEKIAAICAPTIKPASGVVEGIGDDCAVYEHSPSSVQVATTDILAEHQHFDLLTTPPHHLGSKAISV
NVSDICAMNALPRYALISIAMPQNTPVEMVESLYNGMAHAAKLYGTALVGGDTSSSAAGIVISVTLIGETSKENLALRSG
ASPGDLICVTGALGGAAAGLKVLLREKRIMMDHVQNGEPYNRDIMNNLEEYTEAIQHQLLPSARVDIIEFFHKHQIVPTS
MIDISDGLSSDLRHICQQSMVGARIEEGRIPILSQARHIADEFQEDALDYALGGGEDYQLLFTLKPDNFQAIVEHRDITV
IGKITPKEEGMTLNDIYGMQIDMKEIRGFDHFT

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1786619, Length=352, Percent_Identity=32.6704545454545, Blast_Score=135, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: =2.7.4.16 [H]

Molecular weight: Translated: 38316; Mature: 38185

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKPIADIGEFGLIEKIAAICAPTIKPASGVVEGIGDDCAVYEHSPSSVQVATTDILAE
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEECCCCCEEEEHHHHHHC
HQHFDLLTTPPHHLGSKAISVNVSDICAMNALPRYALISIAMPQNTPVEMVESLYNGMAH
CCCCCEECCCCHHHCCCEEEEEHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
AAKLYGTALVGGDTSSSAAGIVISVTLIGETSKENLALRSGASPGDLICVTGALGGAAAG
HHHHHHHEEECCCCCCCCCEEEEEEEEECCCCCCCEEEECCCCCCCEEEEECCCCCHHHH
LKVLLREKRIMMDHVQNGEPYNRDIMNNLEEYTEAIQHQLLPSARVDIIEFFHKHQIVPT
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
SMIDISDGLSSDLRHICQQSMVGARIEEGRIPILSQARHIADEFQEDALDYALGGGEDYQ
CEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LLFTLKPDNFQAIVEHRDITVIGKITPKEEGMTLNDIYGMQIDMKEIRGFDHFT
EEEEECCCCCEEHEECCCEEEEEECCCCCCCCEEHHCCCCEEEHHHHCCCCCCC
>Mature Secondary Structure 
SLKPIADIGEFGLIEKIAAICAPTIKPASGVVEGIGDDCAVYEHSPSSVQVATTDILAE
CCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEECCCCCEEEEHHHHHHC
HQHFDLLTTPPHHLGSKAISVNVSDICAMNALPRYALISIAMPQNTPVEMVESLYNGMAH
CCCCCEECCCCHHHCCCEEEEEHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
AAKLYGTALVGGDTSSSAAGIVISVTLIGETSKENLALRSGASPGDLICVTGALGGAAAG
HHHHHHHEEECCCCCCCCCEEEEEEEEECCCCCCCEEEECCCCCCCEEEEECCCCCHHHH
LKVLLREKRIMMDHVQNGEPYNRDIMNNLEEYTEAIQHQLLPSARVDIIEFFHKHQIVPT
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
SMIDISDGLSSDLRHICQQSMVGARIEEGRIPILSQARHIADEFQEDALDYALGGGEDYQ
CEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LLFTLKPDNFQAIVEHRDITVIGKITPKEEGMTLNDIYGMQIDMKEIRGFDHFT
EEEEECCCCCEEHEECCCEEEEEECCCCCCCCEEHHCCCCEEEHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377; 8419296 [H]