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The map label for this gene is trpB2 [H]

Identifier: 194333175

GI number: 194333175

Start: 359025

End: 360389

Strand: Reverse

Name: trpB2 [H]

Synonym: Paes_0331

Alternate gene names: 194333175

Gene position: 360389-359025 (Counterclockwise)

Preceding gene: 194333176

Following gene: 194333173

Centisome position: 14.34

GC content: 53.04

Gene sequence:

>1365_bases
ATGAATCAGGAACCGACCAAGATCGTGTTACGTGAGGATGAAATGCCCCGCCAATGGTACAATATCCAGGCAGACCTTCC
TACGCCGTTACCTCCCCCGCTCGGTCTGGATGGCAGTCCTATCAAACCGGAAGATCTGGCGAGGGTCTTTCCAATGAACA
TCATCGAACAGGAGGTCAGCACCGAACGATGGATCACCATTCCCGAACCGGTACAGGACATTCTCAAACTCTGGCGACCA
TCTCCGCTCTATCGCGCAAAACGGCTCGAAAAAGCCCTTGGCACACCAGCAAAAATCTATTATAAAAACGAAGGGGTCTC
ACCTGCCGGCAGTCATAAACCCAATACAGCCATTGCCCAGGCATACTACAACAAAGAGTTCGGAATCAAATACCTCACCA
CGGAAACCGGCGCCGGACAGTGGGGCAGCGCATTGGCGATGAGCTGCAAACTCATCGGCATCGAATGCAAGGTATTCATG
GTACGAATCAGTTTTGACCATAAGCCTTTCAGAAAAATCATGATGAAAACCTGGGGCGCAGATTGTATCCCCAGTCCAAG
CCCCATGACCAATATCGGACGGAAAATTCTGGCCGAACAACCCGACACTCCAGGGAGCCTCGGCATCGCCATCAGCGAAG
CCATCGAACTGGCGGTCGAACGTGAAGATACCCGCTATGCACTCGGCAGCGTGCTGAACCATGTCATGCTCCACCAAAGC
ATCATCGGACTCGAAGCAAAAAAACAATTTGAAAAAATCAGCGCCTACCCCGATATCGTCATCGGATGCGCAGGAGGCGG
GTCAAACTTTGCAGGCATAAGTTTTCCGTTTCTCTACGACAAAATTCACGGCAAGGATATCCGGGTCATTGCAACCGAAC
CGGAAGCATGCCCCACGCTGACCAGAGGCCCCTATGTCTACGATGCCGGTGATGTCGCAAAAATGACGCCACTGCTTGCC
ATGCACAGTCTTGGCCACGGCTTTATTCCTCCGGCCATTCATGCCGGCGGCCTGCGTTACCATGGCATGGCCCCGCTGGT
AAGCCATGTCCTGCACAACGGCCTCATTGAAGCAACAGCATTACCGCAAACAGAATGTTACGAAGCGGCTCTCCTCTTCG
CCCACACAGAAGGCTTTATTCCTGCCCCGGAAACATCACACGCCATAGCACAGACGATTCGGGAAGCCCGACATGCAAAG
GAGGAGGGAAAAGAGAAGGTCATTCTGATGAACTGGTCCGGGCATGGGCTGATGGACCTGCAGGGCTACGATGCGTATAT
GTCGGGAAAACTGGAGGATTATCCGCTGCCGGAAGAACTGCTCAAACAGTCGCTTGCCGCAATCAAGGATCATCCAAAAC
CGTGA

Upstream 100 bases:

>100_bases
ACGCCCGGACAATCTCAAAAGATGAAATATGCGCCTGTCTGACTGCATGCGAATCTTTTTCCTCCCCCCGTTTTCTTTTC
TCACTCTAAATTCATAGTCC

Downstream 100 bases:

>100_bases
AAACACGTCAACGGCCTGCAGCCGACAGTTGAACATATGGCGGGGCAAACATCAATGCCTGCCCGTCTTTTATCGAGAGT
CATTTCCTTCAGAGGTGTAG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MNQEPTKIVLREDEMPRQWYNIQADLPTPLPPPLGLDGSPIKPEDLARVFPMNIIEQEVSTERWITIPEPVQDILKLWRP
SPLYRAKRLEKALGTPAKIYYKNEGVSPAGSHKPNTAIAQAYYNKEFGIKYLTTETGAGQWGSALAMSCKLIGIECKVFM
VRISFDHKPFRKIMMKTWGADCIPSPSPMTNIGRKILAEQPDTPGSLGIAISEAIELAVEREDTRYALGSVLNHVMLHQS
IIGLEAKKQFEKISAYPDIVIGCAGGGSNFAGISFPFLYDKIHGKDIRVIATEPEACPTLTRGPYVYDAGDVAKMTPLLA
MHSLGHGFIPPAIHAGGLRYHGMAPLVSHVLHNGLIEATALPQTECYEAALLFAHTEGFIPAPETSHAIAQTIREARHAK
EEGKEKVILMNWSGHGLMDLQGYDAYMSGKLEDYPLPEELLKQSLAAIKDHPKP

Sequences:

>Translated_454_residues
MNQEPTKIVLREDEMPRQWYNIQADLPTPLPPPLGLDGSPIKPEDLARVFPMNIIEQEVSTERWITIPEPVQDILKLWRP
SPLYRAKRLEKALGTPAKIYYKNEGVSPAGSHKPNTAIAQAYYNKEFGIKYLTTETGAGQWGSALAMSCKLIGIECKVFM
VRISFDHKPFRKIMMKTWGADCIPSPSPMTNIGRKILAEQPDTPGSLGIAISEAIELAVEREDTRYALGSVLNHVMLHQS
IIGLEAKKQFEKISAYPDIVIGCAGGGSNFAGISFPFLYDKIHGKDIRVIATEPEACPTLTRGPYVYDAGDVAKMTPLLA
MHSLGHGFIPPAIHAGGLRYHGMAPLVSHVLHNGLIEATALPQTECYEAALLFAHTEGFIPAPETSHAIAQTIREARHAK
EEGKEKVILMNWSGHGLMDLQGYDAYMSGKLEDYPLPEELLKQSLAAIKDHPKP
>Mature_454_residues
MNQEPTKIVLREDEMPRQWYNIQADLPTPLPPPLGLDGSPIKPEDLARVFPMNIIEQEVSTERWITIPEPVQDILKLWRP
SPLYRAKRLEKALGTPAKIYYKNEGVSPAGSHKPNTAIAQAYYNKEFGIKYLTTETGAGQWGSALAMSCKLIGIECKVFM
VRISFDHKPFRKIMMKTWGADCIPSPSPMTNIGRKILAEQPDTPGSLGIAISEAIELAVEREDTRYALGSVLNHVMLHQS
IIGLEAKKQFEKISAYPDIVIGCAGGGSNFAGISFPFLYDKIHGKDIRVIATEPEACPTLTRGPYVYDAGDVAKMTPLLA
MHSLGHGFIPPAIHAGGLRYHGMAPLVSHVLHNGLIEATALPQTECYEAALLFAHTEGFIPAPETSHAIAQTIREARHAK
EEGKEKVILMNWSGHGLMDLQGYDAYMSGKLEDYPLPEELLKQSLAAIKDHPKP

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=409, Percent_Identity=27.8728606356968, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321412, Length=364, Percent_Identity=31.3186813186813, Blast_Score=126, Evalue=6e-30,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 50089; Mature: 50089

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQEPTKIVLREDEMPRQWYNIQADLPTPLPPPLGLDGSPIKPEDLARVFPMNIIEQEVS
CCCCCCEEEEECCCCCCHHEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
TERWITIPEPVQDILKLWRPSPLYRAKRLEKALGTPAKIYYKNEGVSPAGSHKPNTAIAQ
CCCEEECCHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHH
AYYNKEFGIKYLTTETGAGQWGSALAMSCKLIGIECKVFMVRISFDHKPFRKIMMKTWGA
HHHCCCCCEEEEEECCCCCCCCHHHEEEEEEEEEEEEEEEEEEECCCCHHHHHHHHHCCC
DCIPSPSPMTNIGRKILAEQPDTPGSLGIAISEAIELAVEREDTRYALGSVLNHVMLHQS
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IIGLEAKKQFEKISAYPDIVIGCAGGGSNFAGISFPFLYDKIHGKDIRVIATEPEACPTL
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCCCC
TRGPYVYDAGDVAKMTPLLAMHSLGHGFIPPAIHAGGLRYHGMAPLVSHVLHNGLIEATA
CCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCEEECC
LPQTECYEAALLFAHTEGFIPAPETSHAIAQTIREARHAKEEGKEKVILMNWSGHGLMDL
CCCHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEE
QGYDAYMSGKLEDYPLPEELLKQSLAAIKDHPKP
CCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNQEPTKIVLREDEMPRQWYNIQADLPTPLPPPLGLDGSPIKPEDLARVFPMNIIEQEVS
CCCCCCEEEEECCCCCCHHEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
TERWITIPEPVQDILKLWRPSPLYRAKRLEKALGTPAKIYYKNEGVSPAGSHKPNTAIAQ
CCCEEECCHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHH
AYYNKEFGIKYLTTETGAGQWGSALAMSCKLIGIECKVFMVRISFDHKPFRKIMMKTWGA
HHHCCCCCEEEEEECCCCCCCCHHHEEEEEEEEEEEEEEEEEEECCCCHHHHHHHHHCCC
DCIPSPSPMTNIGRKILAEQPDTPGSLGIAISEAIELAVEREDTRYALGSVLNHVMLHQS
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IIGLEAKKQFEKISAYPDIVIGCAGGGSNFAGISFPFLYDKIHGKDIRVIATEPEACPTL
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCCCC
TRGPYVYDAGDVAKMTPLLAMHSLGHGFIPPAIHAGGLRYHGMAPLVSHVLHNGLIEATA
CCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCEEECC
LPQTECYEAALLFAHTEGFIPAPETSHAIAQTIREARHAKEEGKEKVILMNWSGHGLMDL
CCCHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEE
QGYDAYMSGKLEDYPLPEELLKQSLAAIKDHPKP
CCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]