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The map label for this gene is ispH

Identifier: 193213390

GI number: 193213390

Start: 1920660

End: 1921658

Strand: Direct

Name: ispH

Synonym: Cpar_1751

Alternate gene names: 193213390

Gene position: 1920660-1921658 (Clockwise)

Preceding gene: 193213389

Following gene: 193213391

Centisome position: 83.9

GC content: 59.16

Gene sequence:

>999_bases
GTGAAAATAAATCTTGACAGAACATCATCCGGCTTCTGCATCGGCGTGCAGGGCACGATTCATGTTGCCGAAGAGAAGCT
CAACCAGTCGGGCGAGCTGTACTGCCTCGGCGATGTGGTGCACAACGAGGTGGAGGTCAAGCGGCTCGAAGCGCTCGGCA
TGGAGACCATCGACATTCCGGCGTTTGAAGAACTGCGCGACGCCGAGGTGCTGATCCGCGCCCACGGCGAACCGCCTTCG
ACCTACGAAACCGCACGCCGCAACAACCTCGCAATCACCGACACGACCTGCCCGGTGGTAGCGAAGTTGCAGAAAACCGC
CAAAATGCTGCACCAGCTCGGCTATCAGGTCATCATCTACGGCAAAAAAATTCACCCGGAGGTGATCGGCATCAACGGCC
AGTGCAACGACGAAGGCGTCGTCATCAAACACCCCGACCTGTCCGATGAGGCGGAAATCGCGCCGCTCGACCTGGGCCGC
AAGACCGCGTTGATCTCCCAGACCACGATGGATGTACCCGGCTTTTACGAGCTGAAGAAAAACCTCGAAAAGCTTTTCGC
CGAACACGGTCACCGCAATCCTGGCACGAAGAGCGGCGAGTGGATGGCCGTGCGCGACATCGATATCACCTCCGAAAAAA
CCGGCGGTCGCGCCATGCCGGAGCTTGTCTATAAGGACACCATCTGCCGGCAGGTTTCGAGCCGCAACGGTAAGCTGCGC
GATTTCGCGCTCGCGAACGACTGCATCGTCTTTGCGGCGGGTCGCAAAAGCTCGAACGGACAGGTGCTCTACTCGATCTG
CAAAGACGCCAATCCGCACAGCTACTTCATCGAGGATGTCGATGAAATCCAGCCGGAGTGGTTCGTCGGCGAAAACGGCA
AGCCGGTCGAGAGCGTGGGCATCTGCGGCGCGACCTCGACCCCCATGTGGCTGCTCGAAAAGGTGGCCAACTACATCGGC
AAAACCTTCGGCGATGACTCCGGCAACCCGGACGCATGA

Upstream 100 bases:

>100_bases
AACTCCTCCCCACCTCATTATCTTTCCCATTTTAATTGTATCTTTTCCGGCAAAATCTGAACCTTGAAAGGCGACGGCCT
TACCATTCCGGAGAAGTCCA

Downstream 100 bases:

>100_bases
ACGCAACCATGAAATGATACCGACCATGAAACCTGTCAACCTCATTATCGATGGCCGCAGCGTGCAGGCGGCTCCGGGAC
AGACCATCCTCGAAGCGGCT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKINLDRTSSGFCIGVQGTIHVAEEKLNQSGELYCLGDVVHNEVEVKRLEALGMETIDIPAFEELRDAEVLIRAHGEPPS
TYETARRNNLAITDTTCPVVAKLQKTAKMLHQLGYQVIIYGKKIHPEVIGINGQCNDEGVVIKHPDLSDEAEIAPLDLGR
KTALISQTTMDVPGFYELKKNLEKLFAEHGHRNPGTKSGEWMAVRDIDITSEKTGGRAMPELVYKDTICRQVSSRNGKLR
DFALANDCIVFAAGRKSSNGQVLYSICKDANPHSYFIEDVDEIQPEWFVGENGKPVESVGICGATSTPMWLLEKVANYIG
KTFGDDSGNPDA

Sequences:

>Translated_332_residues
MKINLDRTSSGFCIGVQGTIHVAEEKLNQSGELYCLGDVVHNEVEVKRLEALGMETIDIPAFEELRDAEVLIRAHGEPPS
TYETARRNNLAITDTTCPVVAKLQKTAKMLHQLGYQVIIYGKKIHPEVIGINGQCNDEGVVIKHPDLSDEAEIAPLDLGR
KTALISQTTMDVPGFYELKKNLEKLFAEHGHRNPGTKSGEWMAVRDIDITSEKTGGRAMPELVYKDTICRQVSSRNGKLR
DFALANDCIVFAAGRKSSNGQVLYSICKDANPHSYFIEDVDEIQPEWFVGENGKPVESVGICGATSTPMWLLEKVANYIG
KTFGDDSGNPDA
>Mature_332_residues
MKINLDRTSSGFCIGVQGTIHVAEEKLNQSGELYCLGDVVHNEVEVKRLEALGMETIDIPAFEELRDAEVLIRAHGEPPS
TYETARRNNLAITDTTCPVVAKLQKTAKMLHQLGYQVIIYGKKIHPEVIGINGQCNDEGVVIKHPDLSDEAEIAPLDLGR
KTALISQTTMDVPGFYELKKNLEKLFAEHGHRNPGTKSGEWMAVRDIDITSEKTGGRAMPELVYKDTICRQVSSRNGKLR
DFALANDCIVFAAGRKSSNGQVLYSICKDANPHSYFIEDVDEIQPEWFVGENGKPVESVGICGATSTPMWLLEKVANYIG
KTFGDDSGNPDA

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=326, Percent_Identity=26.3803680981595, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_CHLP8 (B3QQE0)

Other databases:

- EMBL:   CP001099
- RefSeq:   YP_001999343.1
- GeneID:   6420694
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_1751
- HOGENOM:   HBG335228
- OMA:   MDVPGFY
- ProtClustDB:   PRK01045
- HAMAP:   MF_00191
- InterPro:   IPR003451

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 36565; Mature: 36565

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINLDRTSSGFCIGVQGTIHVAEEKLNQSGELYCLGDVVHNEVEVKRLEALGMETIDIP
CEEECCCCCCCEEEEECCEEEEEHHHHCCCCCEEEEHHHHCCHHHHHHHHHCCCCEECCC
AFEELRDAEVLIRAHGEPPSTYETARRNNLAITDTTCPVVAKLQKTAKMLHQLGYQVIIY
CHHHHCCCEEEEEECCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEE
GKKIHPEVIGINGQCNDEGVVIKHPDLSDEAEIAPLDLGRKTALISQTTMDVPGFYELKK
CCEECCEEEECCCCCCCCCEEEECCCCCCCCCEEECCCCCCEEEEECCCCCCCCHHHHHH
NLEKLFAEHGHRNPGTKSGEWMAVRDIDITSEKTGGRAMPELVYKDTICRQVSSRNGKLR
HHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
DFALANDCIVFAAGRKSSNGQVLYSICKDANPHSYFIEDVDEIQPEWFVGENGKPVESVG
EEEEECCEEEEEECCCCCCCCEEEEECCCCCCCEEHHHHHHHCCCCEEECCCCCCHHHEE
ICGATSTPMWLLEKVANYIGKTFGDDSGNPDA
ECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKINLDRTSSGFCIGVQGTIHVAEEKLNQSGELYCLGDVVHNEVEVKRLEALGMETIDIP
CEEECCCCCCCEEEEECCEEEEEHHHHCCCCCEEEEHHHHCCHHHHHHHHHCCCCEECCC
AFEELRDAEVLIRAHGEPPSTYETARRNNLAITDTTCPVVAKLQKTAKMLHQLGYQVIIY
CHHHHCCCEEEEEECCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEE
GKKIHPEVIGINGQCNDEGVVIKHPDLSDEAEIAPLDLGRKTALISQTTMDVPGFYELKK
CCEECCEEEECCCCCCCCCEEEECCCCCCCCCEEECCCCCCEEEEECCCCCCCCHHHHHH
NLEKLFAEHGHRNPGTKSGEWMAVRDIDITSEKTGGRAMPELVYKDTICRQVSSRNGKLR
HHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
DFALANDCIVFAAGRKSSNGQVLYSICKDANPHSYFIEDVDEIQPEWFVGENGKPVESVG
EEEEECCEEEEEECCCCCCCCEEEEECCCCCCCEEHHHHHHHCCCCEEECCCCCCHHHEE
ICGATSTPMWLLEKVANYIGKTFGDDSGNPDA
ECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA