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The map label for this gene is infB [H]

Identifier: 190571749

GI number: 190571749

Start: 1473683

End: 1476004

Strand: Reverse

Name: infB [H]

Synonym: WPa_1383

Alternate gene names: 190571749

Gene position: 1476004-1473683 (Counterclockwise)

Preceding gene: 190571750

Following gene: 190571748

Centisome position: 99.56

GC content: 33.12

Gene sequence:

>2322_bases
ATGAATAATGAAGATATCAGTAATAAAAAATTAACCCTACAAGGCTTAAGCAAGCTTAAATTAGACATTAATTTAAACTC
TTTAGATAGTCAAAGTACTGGTACTACAATAGTAAAAAAAAGAAGAAAAATTCATTCTGCAGAAGAATATAGTTTATTTG
ACGAGAGTAAATTAGGCTCTTTAACTAAAAAAGAACAAATTTCCCGCATTAATGCTGTACAGAATGCTACTTTGCTGAAA
GAAAGGAATCAGAGAGAACAAGAAGAGAAAATAAAAAAAGAAGAAAGCAATCAAGAAGTTGAAGATAAAAATGAATCACA
CTCTGTACCTAAAGAAATAAATAAGGAAGTTTTAAGTAATACTAACTCAGTTGAACAAAAAGAAGATAGTATTGAATATG
AGAACAATGATAAAAAGTCTTTTAAAGCTAGCAAAGATATATACTCTAAGCATTCTAAGCTAGTAATTACACAGGCAATA
GATGAAAGAAATGAATACCTCCCTGTATTTAAGCAAAAATTTGGTATAAGAAGTAAACAATCAAAGTTTACTAAGGGTAA
AAATATATCAAGAGAAGTTATTATACCAGACAAAATAACTATCAGGGAGTTATCTATTCGTATGGCAGAAGATAGCAAGA
ATGTGCTAAAAATGCTCAAAGAAGAAGTTGGGGAGAGCTATAGAGTAGATGATCTAGTAGATCCAGATATAGCATGCGAA
ATAGCAAAAAAATTTAGTCATACGGCTAAACGAGTAAGTGATGCTGATAAAGAAAAGAATTTACTCTTCATAAGCAACAG
AGAAAACTTACCTAAAAGACCAAAACCACCTATCGTTACTTTTATGGGACATGTGGATCATGGTAAAACATCATTGCTTG
ATGCATTTCGTGAATCCAATGTTGCAGAAAGAGAGTCAGGTGGCATCACTCAACATATAGGTGCTTATCAATTAACGACA
AAAAATAAGCAAAAAATTACTTTCATTGATACACCAGGGCATGAGGCATTTACTGCAATGCGTGCACGTGGTGCCAATAT
TACTAATATAGTTGTGATAGTAGTTGCAGCTGATGATGGAATCATGAAACAAACAGTTGAAGCAATAAACCATGCAAAAG
CAGCAAATGTCTCTATTATAGTTGCCATTAATAAAATTGATAAATCACAACCTGGCGATGTAGAAAGAATAATTAATAGT
TTGCCTCAGTATGACCTCATCCCCGAAGAACTTGGTGGTGATGTTATAATTGTGCCAGTATCAGCAAAAAAGAAAATCAA
CTTAGATAAACTAGAAGAGGCAATTTTATTAATTGCTGAATTAATGACGCTAGAAGGAATAGAGGATTGTCGAGCACTTG
GATGGGTAATAGAGTCCAAAATAGATAAAGCTAAAGGAATATCAGCTACATTAATAGTTGAAGAAGGAACATTAAAGATT
GGTGACATATTGATAGTTGGTACAACATACAGCAAAGTACGCAGTATGGTTAATCACCTTGGTCAAAGAGAAAAAGCGTC
ACTACCTTCTACTCCAGTTGAAGTTACTGGTTTAAATGGTGTACCAAATGCTGGAGATAAATTTGTTGTTGTAAACTCTG
AAAGGCAGGCTCGTGAAATTGTTGAATACAGGTTAGAATTAATCAAGAAAAAGCAAGAAGATTTAGACGACAGTAATTTA
GATATATTCAGTCGTAATGATAGTGAAACAGAAGAACTATCTGTAGTTTTAAAGTGCGATGTCACTGGTTCTATTGAAGC
AATATCAAGCTCAATTGATAAGCTTGGTAAAGATCAAGTGAAATTAAATATTCTACACAAAGCAGTAGGAGGAGTAACAG
ATTCAGATGTGCTGCTTGCAGAAGCATCAAGCGCAGTAATTTTAGCTTTTAATGTAAAAGTGGATTCAAAAATCAGAGAA
TTAGCAAAACAAAAAGGTATAGAAATACATACTTACAATATAATATATGAGCTTATTGAAGACATGAGGATGTATCTAAC
TAAAATGTTAAAGCCTGTTACACGAGAGGTTCGTGTTGGTTCTGCATCTGTAAGGCAAATATTTAATTCATCCAAAGCCG
GTAATATCATCGGATGTTATGTCACTGATGGAGTTATAAAAAAAGATTCTCTAATTAAGGTAGTACGTGGCAGCAAATTG
GTGCATGAAGGAAAGTTAAAAGCACTACGTAGATTTAAAGATGACGTTAAAGAAGTGGGTGTAAATTTTGAATGCGGAAT
ATCGCTAGAGGGTAATATCGATATTAAAGTTGGAGACATTTTGGAAGCTTATCAATTAGTGCAAGAAGAAAGGACGTTAT
AG

Upstream 100 bases:

>100_bases
ATACTCTCTTCCTCAACTAATAATAAAGACAATCTAAAAGATACAGCAGATTTAATAATCATAGAAGCACGTAAAAAGCT
TGGATTAATATAAAAACAAT

Downstream 100 bases:

>100_bases
TAGTTGAATATGAAAAAGGAAATTAGAAACCTAAAAGTAGCATCGGTATTACATAGGGCAATATCAAAAGTCTTAATGGA
AGGTAATGTTCTTAATGAAA

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 773; Mature: 773

Protein sequence:

>773_residues
MNNEDISNKKLTLQGLSKLKLDINLNSLDSQSTGTTIVKKRRKIHSAEEYSLFDESKLGSLTKKEQISRINAVQNATLLK
ERNQREQEEKIKKEESNQEVEDKNESHSVPKEINKEVLSNTNSVEQKEDSIEYENNDKKSFKASKDIYSKHSKLVITQAI
DERNEYLPVFKQKFGIRSKQSKFTKGKNISREVIIPDKITIRELSIRMAEDSKNVLKMLKEEVGESYRVDDLVDPDIACE
IAKKFSHTAKRVSDADKEKNLLFISNRENLPKRPKPPIVTFMGHVDHGKTSLLDAFRESNVAERESGGITQHIGAYQLTT
KNKQKITFIDTPGHEAFTAMRARGANITNIVVIVVAADDGIMKQTVEAINHAKAANVSIIVAINKIDKSQPGDVERIINS
LPQYDLIPEELGGDVIIVPVSAKKKINLDKLEEAILLIAELMTLEGIEDCRALGWVIESKIDKAKGISATLIVEEGTLKI
GDILIVGTTYSKVRSMVNHLGQREKASLPSTPVEVTGLNGVPNAGDKFVVVNSERQAREIVEYRLELIKKKQEDLDDSNL
DIFSRNDSETEELSVVLKCDVTGSIEAISSSIDKLGKDQVKLNILHKAVGGVTDSDVLLAEASSAVILAFNVKVDSKIRE
LAKQKGIEIHTYNIIYELIEDMRMYLTKMLKPVTREVRVGSASVRQIFNSSKAGNIIGCYVTDGVIKKDSLIKVVRGSKL
VHEGKLKALRRFKDDVKEVGVNFECGISLEGNIDIKVGDILEAYQLVQEERTL

Sequences:

>Translated_773_residues
MNNEDISNKKLTLQGLSKLKLDINLNSLDSQSTGTTIVKKRRKIHSAEEYSLFDESKLGSLTKKEQISRINAVQNATLLK
ERNQREQEEKIKKEESNQEVEDKNESHSVPKEINKEVLSNTNSVEQKEDSIEYENNDKKSFKASKDIYSKHSKLVITQAI
DERNEYLPVFKQKFGIRSKQSKFTKGKNISREVIIPDKITIRELSIRMAEDSKNVLKMLKEEVGESYRVDDLVDPDIACE
IAKKFSHTAKRVSDADKEKNLLFISNRENLPKRPKPPIVTFMGHVDHGKTSLLDAFRESNVAERESGGITQHIGAYQLTT
KNKQKITFIDTPGHEAFTAMRARGANITNIVVIVVAADDGIMKQTVEAINHAKAANVSIIVAINKIDKSQPGDVERIINS
LPQYDLIPEELGGDVIIVPVSAKKKINLDKLEEAILLIAELMTLEGIEDCRALGWVIESKIDKAKGISATLIVEEGTLKI
GDILIVGTTYSKVRSMVNHLGQREKASLPSTPVEVTGLNGVPNAGDKFVVVNSERQAREIVEYRLELIKKKQEDLDDSNL
DIFSRNDSETEELSVVLKCDVTGSIEAISSSIDKLGKDQVKLNILHKAVGGVTDSDVLLAEASSAVILAFNVKVDSKIRE
LAKQKGIEIHTYNIIYELIEDMRMYLTKMLKPVTREVRVGSASVRQIFNSSKAGNIIGCYVTDGVIKKDSLIKVVRGSKL
VHEGKLKALRRFKDDVKEVGVNFECGISLEGNIDIKVGDILEAYQLVQEERTL
>Mature_773_residues
MNNEDISNKKLTLQGLSKLKLDINLNSLDSQSTGTTIVKKRRKIHSAEEYSLFDESKLGSLTKKEQISRINAVQNATLLK
ERNQREQEEKIKKEESNQEVEDKNESHSVPKEINKEVLSNTNSVEQKEDSIEYENNDKKSFKASKDIYSKHSKLVITQAI
DERNEYLPVFKQKFGIRSKQSKFTKGKNISREVIIPDKITIRELSIRMAEDSKNVLKMLKEEVGESYRVDDLVDPDIACE
IAKKFSHTAKRVSDADKEKNLLFISNRENLPKRPKPPIVTFMGHVDHGKTSLLDAFRESNVAERESGGITQHIGAYQLTT
KNKQKITFIDTPGHEAFTAMRARGANITNIVVIVVAADDGIMKQTVEAINHAKAANVSIIVAINKIDKSQPGDVERIINS
LPQYDLIPEELGGDVIIVPVSAKKKINLDKLEEAILLIAELMTLEGIEDCRALGWVIESKIDKAKGISATLIVEEGTLKI
GDILIVGTTYSKVRSMVNHLGQREKASLPSTPVEVTGLNGVPNAGDKFVVVNSERQAREIVEYRLELIKKKQEDLDDSNL
DIFSRNDSETEELSVVLKCDVTGSIEAISSSIDKLGKDQVKLNILHKAVGGVTDSDVLLAEASSAVILAFNVKVDSKIRE
LAKQKGIEIHTYNIIYELIEDMRMYLTKMLKPVTREVRVGSASVRQIFNSSKAGNIIGCYVTDGVIKKDSLIKVVRGSKL
VHEGKLKALRRFKDDVKEVGVNFECGISLEGNIDIKVGDILEAYQLVQEERTL

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family [H]

Homologues:

Organism=Homo sapiens, GI53729339, Length=537, Percent_Identity=40.2234636871508, Blast_Score=381, Evalue=1e-105,
Organism=Homo sapiens, GI53729337, Length=537, Percent_Identity=40.2234636871508, Blast_Score=381, Evalue=1e-105,
Organism=Homo sapiens, GI84043963, Length=467, Percent_Identity=28.4796573875803, Blast_Score=124, Evalue=5e-28,
Organism=Homo sapiens, GI157426893, Length=229, Percent_Identity=28.3842794759825, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789559, Length=606, Percent_Identity=44.2244224422442, Blast_Score=490, Evalue=1e-139,
Organism=Escherichia coli, GI2367247, Length=171, Percent_Identity=30.4093567251462, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994658, Length=542, Percent_Identity=35.239852398524, Blast_Score=317, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI212656558, Length=562, Percent_Identity=22.4199288256228, Blast_Score=103, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17557151, Length=217, Percent_Identity=32.258064516129, Blast_Score=83, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324550, Length=535, Percent_Identity=41.1214953271028, Blast_Score=376, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6319282, Length=131, Percent_Identity=38.9312977099237, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28572034, Length=578, Percent_Identity=38.9273356401384, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI160714833, Length=120, Percent_Identity=50, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24656849, Length=128, Percent_Identity=39.0625, Blast_Score=95, Evalue=2e-19,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N [H]

EC number: NA

Molecular weight: Translated: 86283; Mature: 86283

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNEDISNKKLTLQGLSKLKLDINLNSLDSQSTGTTIVKKRRKIHSAEEYSLFDESKLGS
CCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHCCCCCHHHCC
LTKKEQISRINAVQNATLLKERNQREQEEKIKKEESNQEVEDKNESHSVPKEINKEVLSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHHCC
TNSVEQKEDSIEYENNDKKSFKASKDIYSKHSKLVITQAIDERNEYLPVFKQKFGIRSKQ
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCHHHHHHHCCCCHH
SKFTKGKNISREVIIPDKITIRELSIRMAEDSKNVLKMLKEEVGESYRVDDLVDPDIACE
HHHCCCCCCCCEEECCCCEEEEEHEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
IAKKFSHTAKRVSDADKEKNLLFISNRENLPKRPKPPIVTFMGHVDHGKTSLLDAFRESN
HHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCC
VAERESGGITQHIGAYQLTTKNKQKITFIDTPGHEAFTAMRARGANITNIVVIVVAADDG
CCHHHCCCCHHHCCCEEEECCCCCEEEEEECCCHHHHHHHHHCCCCCCEEEEEEEECCCC
IMKQTVEAINHAKAANVSIIVAINKIDKSQPGDVERIINSLPQYDLIPEELGGDVIIVPV
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCCCCCHHHHCCCEEEEEC
SAKKKINLDKLEEAILLIAELMTLEGIEDCRALGWVIESKIDKAKGISATLIVEEGTLKI
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEE
GDILIVGTTYSKVRSMVNHLGQREKASLPSTPVEVTGLNGVPNAGDKFVVVNSERQAREI
CCEEEEECCHHHHHHHHHHCCCCHHCCCCCCCEEEECCCCCCCCCCEEEEECCCHHHHHH
VEYRLELIKKKQEDLDDSNLDIFSRNDSETEELSVVLKCDVTGSIEAISSSIDKLGKDQV
HHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCHHH
KLNILHKAVGGVTDSDVLLAEASSAVILAFNVKVDSKIRELAKQKGIEIHTYNIIYELIE
HHHHHHHHHCCCCCCCEEEEECCCEEEEEEEECCCHHHHHHHHHCCCEEEHHHHHHHHHH
DMRMYLTKMLKPVTREVRVGSASVRQIFNSSKAGNIIGCYVTDGVIKKDSLIKVVRGSKL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEEECCEECCCHHHHHHHCCCH
VHEGKLKALRRFKDDVKEVGVNFECGISLEGNIDIKVGDILEAYQLVQEERTL
HHHHHHHHHHHHHHHHHHHCCCEEECEEECCCEEEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNEDISNKKLTLQGLSKLKLDINLNSLDSQSTGTTIVKKRRKIHSAEEYSLFDESKLGS
CCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHCCCCCHHHCC
LTKKEQISRINAVQNATLLKERNQREQEEKIKKEESNQEVEDKNESHSVPKEINKEVLSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHHCC
TNSVEQKEDSIEYENNDKKSFKASKDIYSKHSKLVITQAIDERNEYLPVFKQKFGIRSKQ
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCHHHHHHHCCCCHH
SKFTKGKNISREVIIPDKITIRELSIRMAEDSKNVLKMLKEEVGESYRVDDLVDPDIACE
HHHCCCCCCCCEEECCCCEEEEEHEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
IAKKFSHTAKRVSDADKEKNLLFISNRENLPKRPKPPIVTFMGHVDHGKTSLLDAFRESN
HHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCC
VAERESGGITQHIGAYQLTTKNKQKITFIDTPGHEAFTAMRARGANITNIVVIVVAADDG
CCHHHCCCCHHHCCCEEEECCCCCEEEEEECCCHHHHHHHHHCCCCCCEEEEEEEECCCC
IMKQTVEAINHAKAANVSIIVAINKIDKSQPGDVERIINSLPQYDLIPEELGGDVIIVPV
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCCCCCHHHHCCCEEEEEC
SAKKKINLDKLEEAILLIAELMTLEGIEDCRALGWVIESKIDKAKGISATLIVEEGTLKI
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEE
GDILIVGTTYSKVRSMVNHLGQREKASLPSTPVEVTGLNGVPNAGDKFVVVNSERQAREI
CCEEEEECCHHHHHHHHHHCCCCHHCCCCCCCEEEECCCCCCCCCCEEEEECCCHHHHHH
VEYRLELIKKKQEDLDDSNLDIFSRNDSETEELSVVLKCDVTGSIEAISSSIDKLGKDQV
HHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCHHH
KLNILHKAVGGVTDSDVLLAEASSAVILAFNVKVDSKIRELAKQKGIEIHTYNIIYELIE
HHHHHHHHHCCCCCCCEEEEECCCEEEEEEEECCCHHHHHHHHHCCCEEEHHHHHHHHHH
DMRMYLTKMLKPVTREVRVGSASVRQIFNSSKAGNIIGCYVTDGVIKKDSLIKVVRGSKL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEEECCEECCCHHHHHHHCCCH
VHEGKLKALRRFKDDVKEVGVNFECGISLEGNIDIKVGDILEAYQLVQEERTL
HHHHHHHHHHHHHHHHHHHCCCEEECEEECCCEEEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA