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The map label for this gene is mjaIM [H]

Identifier: 189499109

GI number: 189499109

Start: 109124

End: 110071

Strand: Direct

Name: mjaIM [H]

Synonym: Cphamn1_0117

Alternate gene names: 189499109

Gene position: 109124-110071 (Clockwise)

Preceding gene: 189499108

Following gene: 189499110

Centisome position: 3.99

GC content: 49.58

Gene sequence:

>948_bases
TTGCCTTTATCGGTTTCCACGCTTGAAAACCCTCAAACCGATATACCTCGTATTGCAAAGGATCGACAACTGATCAGCAA
AATCGAAGCGGGAGTACAACAGATTGAGACAAATCACAGATTGGTCACTGGAGATGCTCGCAAGTTAGGCTTTATCGAAG
ATGAATCAGTTCATCTTGTGGTTACCTCCCCCCCCTATTGGACACTGAAGAAGTATCGAGATTATGAGAATCAGTTGGGT
GATGTGGCTGATTATGAAGAGTTTCTTACTGAGCTCGACAAGGTTTGGGAACATTGCTACCGGGTTCTTGTTCCGGGCGG
CAGGTTGATTTGCGTTGTCGGTGACGTCTGTCTTTCCAGGAGAAAAAATGACGGACGCCATACTGTCGTGCCTCTTCATG
CGTCCATTCAGGAGCATTGCAGAAAGATGGGTTTCGATAATCTGTCGCCTATTATCTGGCACAAGATTGCAAACGCCTCT
TACGAGGCGAACGGGAACGGCGGGGGTTTCCTGGGAAAACCCTATGAGCCGAATGCAGTGATCAAGAACGATATCGAGTA
CATCCTGATGGAGCGCAAGCCAGGCGGCTATCGTAAACCTTCGGTTGCTACGCGCGTGCTGTCGGTCATATCTGATGAAA
ACCACAGGCAATGGTTTCAGTCAATATGGACTGGTCTTACCGGTGCGTCAACCAGAACTCATCCCGCCCCCTATCCCGAG
GGGTTGGTTGAGCGTCTGATTCGTATGTTTTCCTTTGTCGGCGACACTGTGCTTGATCCCTTTATGGGAACAGGTACGAC
AACAGTAGCGGCTGCGAAATGGGGCAGGAATTCAATCGGTGTGGAAATCGATCCGCATTACTATGCAATGGCCGAGAAGC
GCATTCGTCAGGCAACGGCATCCCTGTTTTCCACGGCGGAAATCGTAACGCAGGCAAAAAGCGTATAA

Upstream 100 bases:

>100_bases
GAATGTTTTTATATTCAAAGATACAGATAACAGTGAATGAAAATATGAATACAGTTTGAATACAATATTTGATATAGCAA
CCAAGACTCACGAGCAGTGT

Downstream 100 bases:

>100_bases
GTATGGATTTGAACACAAGGGTTGCAAAAGCGGTTCGCCACTTCTGGAAAGTAAGAACCCGACAGCACAAGAAGCAGGGT
TCTTCGACGGGAAAAAAAGA

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.MjaI; N-4 cytosine-specific methyltransferase MjaI [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MPLSVSTLENPQTDIPRIAKDRQLISKIEAGVQQIETNHRLVTGDARKLGFIEDESVHLVVTSPPYWTLKKYRDYENQLG
DVADYEEFLTELDKVWEHCYRVLVPGGRLICVVGDVCLSRRKNDGRHTVVPLHASIQEHCRKMGFDNLSPIIWHKIANAS
YEANGNGGGFLGKPYEPNAVIKNDIEYILMERKPGGYRKPSVATRVLSVISDENHRQWFQSIWTGLTGASTRTHPAPYPE
GLVERLIRMFSFVGDTVLDPFMGTGTTTVAAAKWGRNSIGVEIDPHYYAMAEKRIRQATASLFSTAEIVTQAKSV

Sequences:

>Translated_315_residues
MPLSVSTLENPQTDIPRIAKDRQLISKIEAGVQQIETNHRLVTGDARKLGFIEDESVHLVVTSPPYWTLKKYRDYENQLG
DVADYEEFLTELDKVWEHCYRVLVPGGRLICVVGDVCLSRRKNDGRHTVVPLHASIQEHCRKMGFDNLSPIIWHKIANAS
YEANGNGGGFLGKPYEPNAVIKNDIEYILMERKPGGYRKPSVATRVLSVISDENHRQWFQSIWTGLTGASTRTHPAPYPE
GLVERLIRMFSFVGDTVLDPFMGTGTTTVAAAKWGRNSIGVEIDPHYYAMAEKRIRQATASLFSTAEIVTQAKSV
>Mature_314_residues
PLSVSTLENPQTDIPRIAKDRQLISKIEAGVQQIETNHRLVTGDARKLGFIEDESVHLVVTSPPYWTLKKYRDYENQLGD
VADYEEFLTELDKVWEHCYRVLVPGGRLICVVGDVCLSRRKNDGRHTVVPLHASIQEHCRKMGFDNLSPIIWHKIANASY
EANGNGGGFLGKPYEPNAVIKNDIEYILMERKPGGYRKPSVATRVLSVISDENHRQWFQSIWTGLTGASTRTHPAPYPEG
LVERLIRMFSFVGDTVLDPFMGTGTTTVAAAKWGRNSIGVEIDPHYYAMAEKRIRQATASLFSTAEIVTQAKSV

Specific function: This methylase recognizes the double-stranded sequence CTAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the MjaI endonuclease (Potential) [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=286, Percent_Identity=28.6713286713287, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 35371; Mature: 35239

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSVSTLENPQTDIPRIAKDRQLISKIEAGVQQIETNHRLVTGDARKLGFIEDESVHLV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHCCCCCCCCEEEE
VTSPPYWTLKKYRDYENQLGDVADYEEFLTELDKVWEHCYRVLVPGGRLICVVGDVCLSR
EECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHC
RKNDGRHTVVPLHASIQEHCRKMGFDNLSPIIWHKIANASYEANGNGGGFLGKPYEPNAV
CCCCCCEEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
IKNDIEYILMERKPGGYRKPSVATRVLSVISDENHRQWFQSIWTGLTGASTRTHPAPYPE
HHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCHH
GLVERLIRMFSFVGDTVLDPFMGTGTTTVAAAKWGRNSIGVEIDPHYYAMAEKRIRQATA
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHH
SLFSTAEIVTQAKSV
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
PLSVSTLENPQTDIPRIAKDRQLISKIEAGVQQIETNHRLVTGDARKLGFIEDESVHLV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHCCCCCCCCEEEE
VTSPPYWTLKKYRDYENQLGDVADYEEFLTELDKVWEHCYRVLVPGGRLICVVGDVCLSR
EECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHC
RKNDGRHTVVPLHASIQEHCRKMGFDNLSPIIWHKIANASYEANGNGGGFLGKPYEPNAV
CCCCCCEEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
IKNDIEYILMERKPGGYRKPSVATRVLSVISDENHRQWFQSIWTGLTGASTRTHPAPYPE
HHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCHH
GLVERLIRMFSFVGDTVLDPFMGTGTTTVAAAKWGRNSIGVEIDPHYYAMAEKRIRQATA
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHH
SLFSTAEIVTQAKSV
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]