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The map label for this gene is bstVIR [H]

Identifier: 189499110

GI number: 189499110

Start: 110074

End: 110799

Strand: Direct

Name: bstVIR [H]

Synonym: Cphamn1_0118

Alternate gene names: 189499110

Gene position: 110074-110799 (Clockwise)

Preceding gene: 189499109

Following gene: 189499111

Centisome position: 4.02

GC content: 47.8

Gene sequence:

>726_bases
ATGGATTTGAACACAAGGGTTGCAAAAGCGGTTCGCCACTTCTGGAAAGTAAGAACCCGACAGCACAAGAAGCAGGGTTC
TTCGACGGGAAAAAAAGACGCAGGGAACCGGAGTGCGGTAACGGGTGGCAAGCACCTCGATGGATTTATCAAATTGTTGA
CCGAGTTGCTTGCTGAGGCCGGGTTGCCCGATTCTGCAATCCATGTCAAGTCAACTACGTTGCCGGGATATTTTAGGCCA
ACCAAGAATTGGGACTTGCTGGTTGTTGTTGACAGAATACTACTGGCTTCGATTGAACTGAAAGCTCATATCGGCCCGTC
ATTTGGCAACAATTTTAATAACAGGGTTGAAGAAGCTCTTGGTAACTCAACCGATCTGCTTACCGCATACCGCGAAGGAA
AATTCAAGCCTTCGCAAAAGCCTTGGCTTGGCTGGCTGATGCTGCTGGAAGATGCTCCAAAATCAACAACTCCGGTTCGG
GTTGACGAACCGCATTTCGAGGTTTTTCCTGAGTTCAAAGAAGCTTCATACGCAAAACGATACGGGCTCTTTTGCGAACG
GCTTATGCGTGAGCGTTTATATGATGGAGCTTGCCTCATAATGTCCGACAAGGCCGGCGGACTCAAAGGCAAATTCACCG
AGCCGAATTCTGAATTTAGTTTTACGATGTTTGCAACTTCGCTGACAGCACATGCTTTGGCATATGCACGGTTACGCAAA
CTTTAA

Upstream 100 bases:

>100_bases
ATCCGCATTACTATGCAATGGCCGAGAAGCGCATTCGTCAGGCAACGGCATCCCTGTTTTCCACGGCGGAAATCGTAACG
CAGGCAAAAAGCGTATAAGT

Downstream 100 bases:

>100_bases
GGTGGCGAATATGCAAAGATTCTTCTTGATTTGAAAAGGGTGTATATATGGGGAAGATTTTTGAAATTAGGGATCCAATT
CACGGTTTTGTGACCTTGAA

Product: Type II site-specific deoxyribonuclease

Products: NA

Alternate protein names: R.BstVI; Endonuclease BstVI; Type II restriction enzyme BstVI [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MDLNTRVAKAVRHFWKVRTRQHKKQGSSTGKKDAGNRSAVTGGKHLDGFIKLLTELLAEAGLPDSAIHVKSTTLPGYFRP
TKNWDLLVVVDRILLASIELKAHIGPSFGNNFNNRVEEALGNSTDLLTAYREGKFKPSQKPWLGWLMLLEDAPKSTTPVR
VDEPHFEVFPEFKEASYAKRYGLFCERLMRERLYDGACLIMSDKAGGLKGKFTEPNSEFSFTMFATSLTAHALAYARLRK
L

Sequences:

>Translated_241_residues
MDLNTRVAKAVRHFWKVRTRQHKKQGSSTGKKDAGNRSAVTGGKHLDGFIKLLTELLAEAGLPDSAIHVKSTTLPGYFRP
TKNWDLLVVVDRILLASIELKAHIGPSFGNNFNNRVEEALGNSTDLLTAYREGKFKPSQKPWLGWLMLLEDAPKSTTPVR
VDEPHFEVFPEFKEASYAKRYGLFCERLMRERLYDGACLIMSDKAGGLKGKFTEPNSEFSFTMFATSLTAHALAYARLRK
L
>Mature_241_residues
MDLNTRVAKAVRHFWKVRTRQHKKQGSSTGKKDAGNRSAVTGGKHLDGFIKLLTELLAEAGLPDSAIHVKSTTLPGYFRP
TKNWDLLVVVDRILLASIELKAHIGPSFGNNFNNRVEEALGNSTDLLTAYREGKFKPSQKPWLGWLMLLEDAPKSTTPVR
VDEPHFEVFPEFKEASYAKRYGLFCERLMRERLYDGACLIMSDKAGGLKGKFTEPNSEFSFTMFATSLTAHALAYARLRK
L

Specific function: Recognizes the double-stranded sequence CTCGAG and cleaves after C-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II restriction enzyme XhoI family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007636 [H]

Pfam domain/function: PF04555 XhoI [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 27091; Mature: 27091

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLNTRVAKAVRHFWKVRTRQHKKQGSSTGKKDAGNRSAVTGGKHLDGFIKLLTELLAEA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GLPDSAIHVKSTTLPGYFRPTKNWDLLVVVDRILLASIELKAHIGPSFGNNFNNRVEEAL
CCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHH
GNSTDLLTAYREGKFKPSQKPWLGWLMLLEDAPKSTTPVRVDEPHFEVFPEFKEASYAKR
CCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCEEECCCHHHHCCCHHHHHHHHH
YGLFCERLMRERLYDGACLIMSDKAGGLKGKFTEPNSEFSFTMFATSLTAHALAYARLRK
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHC
L
C
>Mature Secondary Structure
MDLNTRVAKAVRHFWKVRTRQHKKQGSSTGKKDAGNRSAVTGGKHLDGFIKLLTELLAEA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GLPDSAIHVKSTTLPGYFRPTKNWDLLVVVDRILLASIELKAHIGPSFGNNFNNRVEEAL
CCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHH
GNSTDLLTAYREGKFKPSQKPWLGWLMLLEDAPKSTTPVRVDEPHFEVFPEFKEASYAKR
CCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCEEECCCHHHHCCCHHHHHHHHH
YGLFCERLMRERLYDGACLIMSDKAGGLKGKFTEPNSEFSFTMFATSLTAHALAYARLRK
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8370531 [H]