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The map label for this gene is recF

Identifier: 189499003

GI number: 189499003

Start: 2914

End: 4005

Strand: Direct

Name: recF

Synonym: Cphamn1_0003

Alternate gene names: 189499003

Gene position: 2914-4005 (Clockwise)

Preceding gene: 189499002

Following gene: 189499004

Centisome position: 0.11

GC content: 44.05

Gene sequence:

>1092_bases
TTGCGATTACAGGAAATACAACTTGTCAATTTCAGAAAGCATAAGGAGCTTGTATTTGCTCCGTCAGAAGCGATTACTGT
TGTCTATGGGCCGAACGGTTCAGGTAAAACAAATATACTTGAGGCGGTTCACTACTGTACGCTTGCAAGAGGATTGAACA
GGTCACTCGACAGGGAGTGCCTCAATTTTGATGCAGGTTACTTTCTGCTACAAGGTACTTTTGCTGATGATAGAGGTATT
GAGCTTTCTGTCAAGGTCAGTTACGAAAAAAACGTTGAGAAAAAAATATTTATCAACAGTGATGAGCTGAAAAAATATTC
TCAACTTATCGGGCGTATTCCATGTGTTACCTTTTCTCCCATGGAGCTTTCTTTGGTAAACGGATCTCCACAGGAGCGAA
GACGGTTTATGGATAACGCTCTTTCCCAGACCAATAAAAGTTATCTCGATGATCTGCTGCAGTACAGAAGAGTATTGCAA
CAGCGTAATACGCTGCTTGGTGCAGTCAACGAAAAGGGAATGGACAGGGATAGCCTGGAGGTATGGACTGAAAAGCTCAC
GCAACTTGCCTGTTCGATCGTCTCGGAAAGACTTGCCTTTATCGAACGATTGTTTGTTTACATAGAGCCTGTCTATGAAC
AGCTTGGTCTCGGGGAAGTTCCAGGTATATCCTATCGCTCCGCTGCAGGAAGACACGCAAACGAAATCGCGCCTGAAGAA
CTTTTTTCCTTCATGATGCAACGATTTCGTGAGATAGAGCATCAGGAAATATTCCGGAAACAGTCGCTTGCAGGACCTCA
CAGAGACGATCTTGTGTTCAGGTTCAATGATACTGATGTGAAAAAATATGCCTCACAGGGGCAGTTGAGGACGTTTCTCA
TTGCCGTAAAGCTCGCTTTACATACACTTGTCAGCGATACTACAGGAGAACGCCCTTTATTTCTCCTTGATGATCTGTTC
AGCGAGCTTGACGGAACAAGGATTGAAAAAATTCTGGAACAGCTTGAAGGAGCCGGTCAATCGATTATCACGGCGACGGA
TAGAAAAGATGGCTCAGGTGTTCGCTCTGTCTCCATAGAAGATCTTTTGTAA

Upstream 100 bases:

>100_bases
ACAGCTGTAATTCTAAAACCGGAGAAAGAGAAAGAAAATGAAAATATGATGATTCTTGTTATGCCGGTAAGAATAAACAA
TTGAGAGGATGGACCGATAG

Downstream 100 bases:

>100_bases
ATGCACTCCTGAAGAGCAAGATTTCCTGACCGTATCGGTGATGTACAAACAACGAGTTCTTCCTACAGTCATAAGCTCAA
TTGATGAAAACCGGGAATAC

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MRLQEIQLVNFRKHKELVFAPSEAITVVYGPNGSGKTNILEAVHYCTLARGLNRSLDRECLNFDAGYFLLQGTFADDRGI
ELSVKVSYEKNVEKKIFINSDELKKYSQLIGRIPCVTFSPMELSLVNGSPQERRRFMDNALSQTNKSYLDDLLQYRRVLQ
QRNTLLGAVNEKGMDRDSLEVWTEKLTQLACSIVSERLAFIERLFVYIEPVYEQLGLGEVPGISYRSAAGRHANEIAPEE
LFSFMMQRFREIEHQEIFRKQSLAGPHRDDLVFRFNDTDVKKYASQGQLRTFLIAVKLALHTLVSDTTGERPLFLLDDLF
SELDGTRIEKILEQLEGAGQSIITATDRKDGSGVRSVSIEDLL

Sequences:

>Translated_363_residues
MRLQEIQLVNFRKHKELVFAPSEAITVVYGPNGSGKTNILEAVHYCTLARGLNRSLDRECLNFDAGYFLLQGTFADDRGI
ELSVKVSYEKNVEKKIFINSDELKKYSQLIGRIPCVTFSPMELSLVNGSPQERRRFMDNALSQTNKSYLDDLLQYRRVLQ
QRNTLLGAVNEKGMDRDSLEVWTEKLTQLACSIVSERLAFIERLFVYIEPVYEQLGLGEVPGISYRSAAGRHANEIAPEE
LFSFMMQRFREIEHQEIFRKQSLAGPHRDDLVFRFNDTDVKKYASQGQLRTFLIAVKLALHTLVSDTTGERPLFLLDDLF
SELDGTRIEKILEQLEGAGQSIITATDRKDGSGVRSVSIEDLL
>Mature_363_residues
MRLQEIQLVNFRKHKELVFAPSEAITVVYGPNGSGKTNILEAVHYCTLARGLNRSLDRECLNFDAGYFLLQGTFADDRGI
ELSVKVSYEKNVEKKIFINSDELKKYSQLIGRIPCVTFSPMELSLVNGSPQERRRFMDNALSQTNKSYLDDLLQYRRVLQ
QRNTLLGAVNEKGMDRDSLEVWTEKLTQLACSIVSERLAFIERLFVYIEPVYEQLGLGEVPGISYRSAAGRHANEIAPEE
LFSFMMQRFREIEHQEIFRKQSLAGPHRDDLVFRFNDTDVKKYASQGQLRTFLIAVKLALHTLVSDTTGERPLFLLDDLF
SELDGTRIEKILEQLEGAGQSIITATDRKDGSGVRSVSIEDLL

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=337, Percent_Identity=25.2225519287834, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_CHLPB (B3EJI1)

Other databases:

- EMBL:   CP001101
- RefSeq:   YP_001958473.1
- GeneID:   6373645
- GenomeReviews:   CP001101_GR
- KEGG:   cpb:Cphamn1_0003
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   CLSK2339234
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- TIGRFAMs:   TIGR00611

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 41372; Mature: 41372

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLQEIQLVNFRKHKELVFAPSEAITVVYGPNGSGKTNILEAVHYCTLARGLNRSLDREC
CCCCHHHHHHHHHCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHH
LNFDAGYFLLQGTFADDRGIELSVKVSYEKNVEKKIFINSDELKKYSQLIGRIPCVTFSP
HCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCEEEEECHHHHHHHHHHHHCCCCEEECC
MELSLVNGSPQERRRFMDNALSQTNKSYLDDLLQYRRVLQQRNTLLGAVNEKGMDRDSLE
CEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
VWTEKLTQLACSIVSERLAFIERLFVYIEPVYEQLGLGEVPGISYRSAAGRHANEIAPEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCHHH
LFSFMMQRFREIEHQEIFRKQSLAGPHRDDLVFRFNDTDVKKYASQGQLRTFLIAVKLAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHH
HTLVSDTTGERPLFLLDDLFSELDGTRIEKILEQLEGAGQSIITATDRKDGSGVRSVSIE
HHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEHH
DLL
HCC
>Mature Secondary Structure
MRLQEIQLVNFRKHKELVFAPSEAITVVYGPNGSGKTNILEAVHYCTLARGLNRSLDREC
CCCCHHHHHHHHHCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHH
LNFDAGYFLLQGTFADDRGIELSVKVSYEKNVEKKIFINSDELKKYSQLIGRIPCVTFSP
HCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCEEEEECHHHHHHHHHHHHCCCCEEECC
MELSLVNGSPQERRRFMDNALSQTNKSYLDDLLQYRRVLQQRNTLLGAVNEKGMDRDSLE
CEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
VWTEKLTQLACSIVSERLAFIERLFVYIEPVYEQLGLGEVPGISYRSAAGRHANEIAPEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCHHH
LFSFMMQRFREIEHQEIFRKQSLAGPHRDDLVFRFNDTDVKKYASQGQLRTFLIAVKLAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHH
HTLVSDTTGERPLFLLDDLFSELDGTRIEKILEQLEGAGQSIITATDRKDGSGVRSVSIE
HHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEHH
DLL
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA