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The map label for this gene is dnaN [H]

Identifier: 189499002

GI number: 189499002

Start: 1773

End: 2897

Strand: Direct

Name: dnaN [H]

Synonym: Cphamn1_0002

Alternate gene names: 189499002

Gene position: 1773-2897 (Clockwise)

Preceding gene: 189499001

Following gene: 189499003

Centisome position: 0.06

GC content: 41.51

Gene sequence:

>1125_bases
ATGAAATTTACGACCTCTATCAAACATTTTCAGGAAGCTGTCAACAAGGCGATTCAGGCTATCCCGAACAAAGTTCTTGA
TCCCCGTTTCGAAAATCTTCATCTGAATATCGATAACCGGATACTGACACTTTTTGCAACGGACGGCGAAATATCCATAA
CAGCAACAACTGATGTTGATTCTTCCGATAAGGGTGATCTGGGAATGAGAGCTCGAACTATCCTTGATTTTCTGAGGAGC
ATGTATGACACTGAAGTCAATTTCGAAATTGAACGTCAGCAGATGAGCGAACAGGGAATCGTCAATATAGCTACCGATAA
AGGGAGGTATACCATTCCCTGCAGCTTTGAAAGTAAAGCTGAAAAACAGGAGAAAACATTTGATATAGAGTTTGACCTGA
CTGCCGGAGATCTGCTCAATATCATTCAGAAAACAACGTTTGCATGTAGTATCGATGGTATGAGACCGGCAATGATGGGG
GTACTTTTTGAAATAGAGCAGAATCTCCTTTCAGCTATTGCAACGGACGGACACCGTCTTGTCAGGTTCAGAAAAACATG
TGAAGTTTCTCTTTCTGAAAAGCAGAGAATCGTCATACCAGCCAGAGTTCTCTCTATTCTGCAAAAGCTTGTCAGCGAAG
GAAATATCAGAATATCAATAGATTCGGCCAACCGATCAATACGTTTTTCTACAGAGAGTCTCGTTCTTGATGCTGCCTTG
ATTGTCGAGCAGTATCCTAACTATGAAGCTGTCATTCCGCTTGAAAACGATAAAAAGCTGAGTATTGAAAGAGCTTCTTT
CTATGATTCTGTGAAAAGGGTGGGCAGATTCTCGAATATAGGTGATATACGATTCAGCATTTCTCCTTCAACCATGAAAC
TGACGGCGGAGAATATAAATGAAGGGGAATCGGCACAGGAGGATCTTGCCTGCTCCTATGATAACGAGCCCATAGAGATC
GGTTTCAATTCGAAATTCATCGAAGCGGCACTTGCGCATATCGATGACGATTCGCTTATCGTCGAACTGAGCACTCCAAC
AACAGCTGTAATTCTAAAACCGGAGAAAGAGAAAGAAAATGAAAATATGATGATTCTTGTTATGCCGGTAAGAATAAACA
ATTGA

Upstream 100 bases:

>100_bases
TTTAAGTAGCTTACAACAAAAAAACCTGAGTAATCAAGATTTCCACACACTCAACAACAACAACAACATTTTTAATACCA
AATAATAAAGAAGCTATACA

Downstream 100 bases:

>100_bases
GAGGATGGACCGATAGTTGCGATTACAGGAAATACAACTTGTCAATTTCAGAAAGCATAAGGAGCTTGTATTTGCTCCGT
CAGAAGCGATTACTGTTGTC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKFTTSIKHFQEAVNKAIQAIPNKVLDPRFENLHLNIDNRILTLFATDGEISITATTDVDSSDKGDLGMRARTILDFLRS
MYDTEVNFEIERQQMSEQGIVNIATDKGRYTIPCSFESKAEKQEKTFDIEFDLTAGDLLNIIQKTTFACSIDGMRPAMMG
VLFEIEQNLLSAIATDGHRLVRFRKTCEVSLSEKQRIVIPARVLSILQKLVSEGNIRISIDSANRSIRFSTESLVLDAAL
IVEQYPNYEAVIPLENDKKLSIERASFYDSVKRVGRFSNIGDIRFSISPSTMKLTAENINEGESAQEDLACSYDNEPIEI
GFNSKFIEAALAHIDDDSLIVELSTPTTAVILKPEKEKENENMMILVMPVRINN

Sequences:

>Translated_374_residues
MKFTTSIKHFQEAVNKAIQAIPNKVLDPRFENLHLNIDNRILTLFATDGEISITATTDVDSSDKGDLGMRARTILDFLRS
MYDTEVNFEIERQQMSEQGIVNIATDKGRYTIPCSFESKAEKQEKTFDIEFDLTAGDLLNIIQKTTFACSIDGMRPAMMG
VLFEIEQNLLSAIATDGHRLVRFRKTCEVSLSEKQRIVIPARVLSILQKLVSEGNIRISIDSANRSIRFSTESLVLDAAL
IVEQYPNYEAVIPLENDKKLSIERASFYDSVKRVGRFSNIGDIRFSISPSTMKLTAENINEGESAQEDLACSYDNEPIEI
GFNSKFIEAALAHIDDDSLIVELSTPTTAVILKPEKEKENENMMILVMPVRINN
>Mature_374_residues
MKFTTSIKHFQEAVNKAIQAIPNKVLDPRFENLHLNIDNRILTLFATDGEISITATTDVDSSDKGDLGMRARTILDFLRS
MYDTEVNFEIERQQMSEQGIVNIATDKGRYTIPCSFESKAEKQEKTFDIEFDLTAGDLLNIIQKTTFACSIDGMRPAMMG
VLFEIEQNLLSAIATDGHRLVRFRKTCEVSLSEKQRIVIPARVLSILQKLVSEGNIRISIDSANRSIRFSTESLVLDAAL
IVEQYPNYEAVIPLENDKKLSIERASFYDSVKRVGRFSNIGDIRFSISPSTMKLTAENINEGESAQEDLACSYDNEPIEI
GFNSKFIEAALAHIDDDSLIVELSTPTTAVILKPEKEKENENMMILVMPVRINN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=385, Percent_Identity=24.4155844155844, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42062; Mature: 42062

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTTSIKHFQEAVNKAIQAIPNKVLDPRFENLHLNIDNRILTLFATDGEISITATTDVD
CCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEECCEEEEEEEECCEEEEEEECCCC
SSDKGDLGMRARTILDFLRSMYDTEVNFEIERQQMSEQGIVNIATDKGRYTIPCSFESKA
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCEEEEECCCCEEEEECCCCCC
EKQEKTFDIEFDLTAGDLLNIIQKTTFACSIDGMRPAMMGVLFEIEQNLLSAIATDGHRL
CHHHCEEEEEEECCHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHH
VRFRKTCEVSLSEKQRIVIPARVLSILQKLVSEGNIRISIDSANRSIRFSTESLVLDAAL
EEEHHHEEEECCCCCEEEEHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEHHHHHHHHHH
IVEQYPNYEAVIPLENDKKLSIERASFYDSVKRVGRFSNIGDIRFSISPSTMKLTAENIN
HHHHCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCC
EGESAQEDLACSYDNEPIEIGFNSKFIEAALAHIDDDSLIVELSTPTTAVILKPEKEKEN
CCCCCHHCCEECCCCCCEEECCCHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCCC
ENMMILVMPVRINN
CCEEEEEEEEEECC
>Mature Secondary Structure
MKFTTSIKHFQEAVNKAIQAIPNKVLDPRFENLHLNIDNRILTLFATDGEISITATTDVD
CCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEECCEEEEEEEECCEEEEEEECCCC
SSDKGDLGMRARTILDFLRSMYDTEVNFEIERQQMSEQGIVNIATDKGRYTIPCSFESKA
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCEEEEECCCCEEEEECCCCCC
EKQEKTFDIEFDLTAGDLLNIIQKTTFACSIDGMRPAMMGVLFEIEQNLLSAIATDGHRL
CHHHCEEEEEEECCHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHH
VRFRKTCEVSLSEKQRIVIPARVLSILQKLVSEGNIRISIDSANRSIRFSTESLVLDAAL
EEEHHHEEEECCCCCEEEEHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEHHHHHHHHHH
IVEQYPNYEAVIPLENDKKLSIERASFYDSVKRVGRFSNIGDIRFSISPSTMKLTAENIN
HHHHCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCC
EGESAQEDLACSYDNEPIEIGFNSKFIEAALAHIDDDSLIVELSTPTTAVILKPEKEKEN
CCCCCHHCCEECCCCCCEEECCCHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCCC
ENMMILVMPVRINN
CCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]