| Definition | Orientia tsutsugamushi str. Ikeda, complete genome. |
|---|---|
| Accession | NC_010793 |
| Length | 2,008,987 |
Click here to switch to the map view.
The map label for this gene is murF [H]
Identifier: 189184780
GI number: 189184780
Start: 1912466
End: 1913875
Strand: Reverse
Name: murF [H]
Synonym: OTT_1873
Alternate gene names: 189184780
Gene position: 1913875-1912466 (Counterclockwise)
Preceding gene: 189184781
Following gene: 189184779
Centisome position: 95.27
GC content: 29.86
Gene sequence:
>1410_bases ATGAAATGCAATATATGGTCAGCTGCAGATTTAACAGCAGCACTAGGAGTGCCAGTAAATGTTAATGTTAATGGCATCGT CCAGTTTAATTCTAAAGATATACAGCCAGGTGATTTATTTATTGCGCTGCAGTCTGAAAATGGAGGTGACGGACACCAAT TTGTTAAGGATGCTTTAGCTCGTGGCGCTGATGCAGTAATAGTAAGCAAGTTAATTGATAACAATACAAATGATAAAATC ATATTAGTCAATAATACAATGAGTGCGTTATCACAGCTAGCAAATTATCGACGTCAGCAGTTAAAAAATACTACGTTTAT TGGTATTACAGGCAGCGTTGGCAAGACATCTACAAAAGAAATCATGTTTAAGGTTTTTAGTACACATTATAAAACTTTTG CAAGTCGCAAAAATTTCAACAATTTACTTGGTATGCTACTAAATCTTGCATCAGTGCCAATTAATACTGAATATGCTATT TTTGAATTATGCACAAGGAGTCCTGGCGAACTTCAAAAATTAGTTAAAATTTTACCATTAAATATTGCTGTACTTACTAA TATTAATTCAGCTCATACTGAATTTTTTAATTCACTAGAACATTTAACAGAGGCTAAATGTGAAATTTTTAATGGATTAG TTAACAAAGGTATAGCAGTTATTAATTCTGACAGCATGTTTTTCACTAAACAGCTAGAATACATTAATAAACTTGGTATT AATAATGTGTTTACCTTTGGAACACATCAATTATCTAATAGTAGGATTTTAAACTATTATTATGATGCTGATAACCAAAA ATCAGTAATAAATATTAATTGTAATGGCTATGATTTGCAATTTTATACTCAAATTCTAGGCTATGGTCAAGCATTTAACA TTGCTGCAGTAATGTTAGTAAGTAATATTTGTAAAATAAATCTAAATAAGGTAACAGAAGTAATTAATACACTTAGAAAC TATGAAGGCAGAGGTAAGATTTATTTATTAAATTTTCATTCAAACTCTTCGTTCATAATAGATGATTGTTACAATGCAAA TCCAGCATCAATGAAAAATGCTTTAGATCTATTAGACCAAATTAAGGCTAAAAGAAAAGTAGCTATTTTAGGCGATATGC TAGAGTTAGGCAATAATACTAAAGAACATCATTTAAATCTTGTTACTTACATTAAAAAGTACAATATTCATGTTATTTCT ATTGGCTCTTTGATGAAAAATGTATATGAACTATTACCAGCTGAACTTAAGCTTAGTCATTATAATAATATTAATGAGTT AACGGTTGATCAGTTGTTGCCATTACTACATGATAAAGATTATGTTTTAATTAAAGGATCAAACCGCATGAGGCTTTGGC AGGTCGTCGAAAATTTACTTGAATCTGGAATGTTAAAAGATGCTATATAA
Upstream 100 bases:
>100_bases GTCATGAAACTCGGCAAGTATTAAAAGGTCGTATAATTAATTATAACGACATGGAACAAGTAAAACTTTATCTAAAACAA TTAAACAAGATGAGTAACCA
Downstream 100 bases:
>100_bases TTTACTCGTATCGCATATAAATAGCTGTTATATTTTCAGTATTTTTTATAATGTTATAGTAAGAAGTGGAATAGCTATAT TACTAAGCTTTAGTATATCA
Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6- diaminopimelate--D-alanyl-D-alanyl ligase
Products: NA
Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]
Number of amino acids: Translated: 469; Mature: 469
Protein sequence:
>469_residues MKCNIWSAADLTAALGVPVNVNVNGIVQFNSKDIQPGDLFIALQSENGGDGHQFVKDALARGADAVIVSKLIDNNTNDKI ILVNNTMSALSQLANYRRQQLKNTTFIGITGSVGKTSTKEIMFKVFSTHYKTFASRKNFNNLLGMLLNLASVPINTEYAI FELCTRSPGELQKLVKILPLNIAVLTNINSAHTEFFNSLEHLTEAKCEIFNGLVNKGIAVINSDSMFFTKQLEYINKLGI NNVFTFGTHQLSNSRILNYYYDADNQKSVININCNGYDLQFYTQILGYGQAFNIAAVMLVSNICKINLNKVTEVINTLRN YEGRGKIYLLNFHSNSSFIIDDCYNANPASMKNALDLLDQIKAKRKVAILGDMLELGNNTKEHHLNLVTYIKKYNIHVIS IGSLMKNVYELLPAELKLSHYNNINELTVDQLLPLLHDKDYVLIKGSNRMRLWQVVENLLESGMLKDAI
Sequences:
>Translated_469_residues MKCNIWSAADLTAALGVPVNVNVNGIVQFNSKDIQPGDLFIALQSENGGDGHQFVKDALARGADAVIVSKLIDNNTNDKI ILVNNTMSALSQLANYRRQQLKNTTFIGITGSVGKTSTKEIMFKVFSTHYKTFASRKNFNNLLGMLLNLASVPINTEYAI FELCTRSPGELQKLVKILPLNIAVLTNINSAHTEFFNSLEHLTEAKCEIFNGLVNKGIAVINSDSMFFTKQLEYINKLGI NNVFTFGTHQLSNSRILNYYYDADNQKSVININCNGYDLQFYTQILGYGQAFNIAAVMLVSNICKINLNKVTEVINTLRN YEGRGKIYLLNFHSNSSFIIDDCYNANPASMKNALDLLDQIKAKRKVAILGDMLELGNNTKEHHLNLVTYIKKYNIHVIS IGSLMKNVYELLPAELKLSHYNNINELTVDQLLPLLHDKDYVLIKGSNRMRLWQVVENLLESGMLKDAI >Mature_469_residues MKCNIWSAADLTAALGVPVNVNVNGIVQFNSKDIQPGDLFIALQSENGGDGHQFVKDALARGADAVIVSKLIDNNTNDKI ILVNNTMSALSQLANYRRQQLKNTTFIGITGSVGKTSTKEIMFKVFSTHYKTFASRKNFNNLLGMLLNLASVPINTEYAI FELCTRSPGELQKLVKILPLNIAVLTNINSAHTEFFNSLEHLTEAKCEIFNGLVNKGIAVINSDSMFFTKQLEYINKLGI NNVFTFGTHQLSNSRILNYYYDADNQKSVININCNGYDLQFYTQILGYGQAFNIAAVMLVSNICKINLNKVTEVINTLRN YEGRGKIYLLNFHSNSSFIIDDCYNANPASMKNALDLLDQIKAKRKVAILGDMLELGNNTKEHHLNLVTYIKKYNIHVIS IGSLMKNVYELLPAELKLSHYNNINELTVDQLLPLLHDKDYVLIKGSNRMRLWQVVENLLESGMLKDAI
Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]
COG id: COG0770
COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family [H]
Homologues:
Organism=Escherichia coli, GI1786274, Length=449, Percent_Identity=28.7305122494432, Blast_Score=141, Evalue=9e-35,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR005863 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.10 [H]
Molecular weight: Translated: 52564; Mature: 52564
Theoretical pI: Translated: 8.51; Mature: 8.51
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 3.6 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 3.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKCNIWSAADLTAALGVPVNVNVNGIVQFNSKDIQPGDLFIALQSENGGDGHQFVKDALA CCCCCCCCCCHHHHCCCCEEECEEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHH RGADAVIVSKLIDNNTNDKIILVNNTMSALSQLANYRRQQLKNTTFIGITGSVGKTSTKE CCCCHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHH IMFKVFSTHYKTFASRKNFNNLLGMLLNLASVPINTEYAIFELCTRSPGELQKLVKILPL HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHCC NIAVLTNINSAHTEFFNSLEHLTEAKCEIFNGLVNKGIAVINSDSMFFTKQLEYINKLGI CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHCCC NNVFTFGTHQLSNSRILNYYYDADNQKSVININCNGYDLQFYTQILGYGQAFNIAAVMLV CCEEECCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHH SNICKINLNKVTEVINTLRNYEGRGKIYLLNFHSNSSFIIDDCYNANPASMKNALDLLDQ HHHHHCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHH IKAKRKVAILGDMLELGNNTKEHHLNLVTYIKKYNIHVISIGSLMKNVYELLPAELKLSH HHHHHHHHHHHHHHHHCCCCHHHHHHHHEEHHHCCEEEEEHHHHHHHHHHHCCHHEEHHH YNNINELTVDQLLPLLHDKDYVLIKGSNRMRLWQVVENLLESGMLKDAI CCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHCCC >Mature Secondary Structure MKCNIWSAADLTAALGVPVNVNVNGIVQFNSKDIQPGDLFIALQSENGGDGHQFVKDALA CCCCCCCCCCHHHHCCCCEEECEEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHH RGADAVIVSKLIDNNTNDKIILVNNTMSALSQLANYRRQQLKNTTFIGITGSVGKTSTKE CCCCHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHH IMFKVFSTHYKTFASRKNFNNLLGMLLNLASVPINTEYAIFELCTRSPGELQKLVKILPL HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHCC NIAVLTNINSAHTEFFNSLEHLTEAKCEIFNGLVNKGIAVINSDSMFFTKQLEYINKLGI CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHCCC NNVFTFGTHQLSNSRILNYYYDADNQKSVININCNGYDLQFYTQILGYGQAFNIAAVMLV CCEEECCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHH SNICKINLNKVTEVINTLRNYEGRGKIYLLNFHSNSSFIIDDCYNANPASMKNALDLLDQ HHHHHCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHH IKAKRKVAILGDMLELGNNTKEHHLNLVTYIKKYNIHVISIGSLMKNVYELLPAELKLSH HHHHHHHHHHHHHHHHCCCCHHHHHHHHEEHHHCCEEEEEHHHHHHHHHHHCCHHEEHHH YNNINELTVDQLLPLLHDKDYVLIKGSNRMRLWQVVENLLESGMLKDAI CCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9823893; 9274032 [H]