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The map label for this gene is murE [H]

Identifier: 189184781

GI number: 189184781

Start: 1913872

End: 1915341

Strand: Reverse

Name: murE [H]

Synonym: OTT_1874

Alternate gene names: 189184781

Gene position: 1915341-1913872 (Counterclockwise)

Preceding gene: 189184782

Following gene: 189184780

Centisome position: 95.34

GC content: 30.41

Gene sequence:

>1470_bases
ATGGAATCAAATAATATCATTGAAATTATTAAAAAGCTAAATATAAGAAATATTGCTATAACAGCTCAATCAGCAAAACC
TAATCAAATATTTTTTGCATTACCAGGAATATCACGTCATGGAAACGATTTTATCGATATTGCTTTAGCAAATGGAGCTA
GTCTAATTATTACTGATCAAGCACCTATAACATCATATAGTAATGTTATAGTAGTAAAAAATATTACACTAGTTATGAAG
GATATAGTTAATTACTTATATCCTAAATTTCCTAAATACTTAGTCGCTGTAACTGGTACTGATGGTAAAACTTCAGTGGT
TAATTATTTTCAACAACTATGTATGTTAGCTGGTGAGCAAGCTGCTAGTATCGGCACATTAGGAGTTATAACTACAAATA
ATCATTTAAATAAGCTATTAAATCAGAATAAGATAAAACCTGAATGTGCTACTACTATGAGCTATATTCAAACACGGTAT
GTCCTACATCAACTTGCTACTAATGGAATTAATTTTGTAGCATTAGAAGCTTCTAGCCATGGCCTTGATCAAAACCGCCT
AATGGACATTAAATTTCAAGCAGCAGCATTTACTAGTTTTAGCCGAGATCATTTAGATTATCATAAAACTATGAATAAAT
ATCTTGATGCTAAGTTAAAGCTTTTTAAAGAAAATATAGTTAGTAATGGCATTGCAATAGTCAACAATAATATTAATGAG
TTATCAATTATTCAATCTAAAATCACTGATGAATTTGGTCTCAAGTTATTAAGTGTAGAACAACAAGGTGATATTCAAAT
TATCGATGTTCAAAGTTCATTAAATGGTCAAAAAGTACATTTTAGGTATAAGAATAGCGATTATAAATTTGAAGTACCAG
TAATAGGCAGAGTTCACATCACTAACATACTAATAGCCTTGTTATTAGCTATTAATATTGGACTTGATTCAGCAAAATTA
ATACCATTACTATCTAATTTAAAACCAATTAAAGGCAGAATGGAGAAAATTCAATTTGGCAATTGTTATGCCTTTGTTGA
TTATGCCCTTACTCCCAATGCATTAGCAACAATGCTAACAGAGCTACGAGCATTAAATAATAAAGGAAGATTAATAACTA
TATTTGGTGGCAGTGAAAATAGAGATGCGATAGCTAGGAGAGTGCAAATGGGAATAGTAGCAAGCAAATTATCTGACTTA
GTAATAGTAACTGATCAAGATTCAGGAAATGAAGATCCAGCTGCGATTAGAAAAGAAATTTTGCAAGTAGCTCGATCAGC
AATTGAAATTCCTAATCGAGGAGAAGCGATAAAATATGCTATCAGTATAATGGTTGACAATGATATACTACTTATAGCAG
GTAAAGGTCATGAAACTCGGCAAGTATTAAAAGGTCGTATAATTAATTATAACGACATGGAACAAGTAAAACTTTATCTA
AAACAATTAAACAAGATGAGTAACCAATGA

Upstream 100 bases:

>100_bases
GCTAAAATTATTTCTAAAATAGGTACGCTATATGGTATACAACCATATCATACAGAAATGACTAGTGTTCAGAATCTTAA
AACCAATAACAACTCTCAGC

Downstream 100 bases:

>100_bases
AATGCAATATATGGTCAGCTGCAGATTTAACAGCAGCACTAGGAGTGCCAGTAAATGTTAATGTTAATGGCATCGTCCAG
TTTAATTCTAAAGATATACA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MESNNIIEIIKKLNIRNIAITAQSAKPNQIFFALPGISRHGNDFIDIALANGASLIITDQAPITSYSNVIVVKNITLVMK
DIVNYLYPKFPKYLVAVTGTDGKTSVVNYFQQLCMLAGEQAASIGTLGVITTNNHLNKLLNQNKIKPECATTMSYIQTRY
VLHQLATNGINFVALEASSHGLDQNRLMDIKFQAAAFTSFSRDHLDYHKTMNKYLDAKLKLFKENIVSNGIAIVNNNINE
LSIIQSKITDEFGLKLLSVEQQGDIQIIDVQSSLNGQKVHFRYKNSDYKFEVPVIGRVHITNILIALLLAINIGLDSAKL
IPLLSNLKPIKGRMEKIQFGNCYAFVDYALTPNALATMLTELRALNNKGRLITIFGGSENRDAIARRVQMGIVASKLSDL
VIVTDQDSGNEDPAAIRKEILQVARSAIEIPNRGEAIKYAISIMVDNDILLIAGKGHETRQVLKGRIINYNDMEQVKLYL
KQLNKMSNQ

Sequences:

>Translated_489_residues
MESNNIIEIIKKLNIRNIAITAQSAKPNQIFFALPGISRHGNDFIDIALANGASLIITDQAPITSYSNVIVVKNITLVMK
DIVNYLYPKFPKYLVAVTGTDGKTSVVNYFQQLCMLAGEQAASIGTLGVITTNNHLNKLLNQNKIKPECATTMSYIQTRY
VLHQLATNGINFVALEASSHGLDQNRLMDIKFQAAAFTSFSRDHLDYHKTMNKYLDAKLKLFKENIVSNGIAIVNNNINE
LSIIQSKITDEFGLKLLSVEQQGDIQIIDVQSSLNGQKVHFRYKNSDYKFEVPVIGRVHITNILIALLLAINIGLDSAKL
IPLLSNLKPIKGRMEKIQFGNCYAFVDYALTPNALATMLTELRALNNKGRLITIFGGSENRDAIARRVQMGIVASKLSDL
VIVTDQDSGNEDPAAIRKEILQVARSAIEIPNRGEAIKYAISIMVDNDILLIAGKGHETRQVLKGRIINYNDMEQVKLYL
KQLNKMSNQ
>Mature_489_residues
MESNNIIEIIKKLNIRNIAITAQSAKPNQIFFALPGISRHGNDFIDIALANGASLIITDQAPITSYSNVIVVKNITLVMK
DIVNYLYPKFPKYLVAVTGTDGKTSVVNYFQQLCMLAGEQAASIGTLGVITTNNHLNKLLNQNKIKPECATTMSYIQTRY
VLHQLATNGINFVALEASSHGLDQNRLMDIKFQAAAFTSFSRDHLDYHKTMNKYLDAKLKLFKENIVSNGIAIVNNNINE
LSIIQSKITDEFGLKLLSVEQQGDIQIIDVQSSLNGQKVHFRYKNSDYKFEVPVIGRVHITNILIALLLAINIGLDSAKL
IPLLSNLKPIKGRMEKIQFGNCYAFVDYALTPNALATMLTELRALNNKGRLITIFGGSENRDAIARRVQMGIVASKLSDL
VIVTDQDSGNEDPAAIRKEILQVARSAIEIPNRGEAIKYAISIMVDNDILLIAGKGHETRQVLKGRIINYNDMEQVKLYL
KQLNKMSNQ

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=476, Percent_Identity=31.0924369747899, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1786274, Length=331, Percent_Identity=23.5649546827795, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 54364; Mature: 54364

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESNNIIEIIKKLNIRNIAITAQSAKPNQIFFALPGISRHGNDFIDIALANGASLIITDQ
CCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCCCEEEEEEECCCEEEEECC
APITSYSNVIVVKNITLVMKDIVNYLYPKFPKYLVAVTGTDGKTSVVNYFQQLCMLAGEQ
CCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
AASIGTLGVITTNNHLNKLLNQNKIKPECATTMSYIQTRYVLHQLATNGINFVALEASSH
CCCCCEEEEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
GLDQNRLMDIKFQAAAFTSFSRDHLDYHKTMNKYLDAKLKLFKENIVSNGIAIVNNNINE
CCCCCCEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCH
LSIIQSKITDEFGLKLLSVEQQGDIQIIDVQSSLNGQKVHFRYKNSDYKFEVPVIGRVHI
HHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCCCCCEEEEEECCCCEEEECCEECHHHH
TNILIALLLAINIGLDSAKLIPLLSNLKPIKGRMEKIQFGNCYAFVDYALTPNALATMLT
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHEECCCEEEEEEECCCHHHHHHHHH
ELRALNNKGRLITIFGGSENRDAIARRVQMGIVASKLSDLVIVTDQDSGNEDPAAIRKEI
HHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
LQVARSAIEIPNRGEAIKYAISIMVDNDILLIAGKGHETRQVLKGRIINYNDMEQVKLYL
HHHHHHHHCCCCCCCEEEEEEEEEECCCEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHH
KQLNKMSNQ
HHHHHHCCC
>Mature Secondary Structure
MESNNIIEIIKKLNIRNIAITAQSAKPNQIFFALPGISRHGNDFIDIALANGASLIITDQ
CCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCCCEEEEEEECCCEEEEECC
APITSYSNVIVVKNITLVMKDIVNYLYPKFPKYLVAVTGTDGKTSVVNYFQQLCMLAGEQ
CCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
AASIGTLGVITTNNHLNKLLNQNKIKPECATTMSYIQTRYVLHQLATNGINFVALEASSH
CCCCCEEEEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
GLDQNRLMDIKFQAAAFTSFSRDHLDYHKTMNKYLDAKLKLFKENIVSNGIAIVNNNINE
CCCCCCEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCH
LSIIQSKITDEFGLKLLSVEQQGDIQIIDVQSSLNGQKVHFRYKNSDYKFEVPVIGRVHI
HHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCCCCCEEEEEECCCCEEEECCEECHHHH
TNILIALLLAINIGLDSAKLIPLLSNLKPIKGRMEKIQFGNCYAFVDYALTPNALATMLT
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHEECCCEEEEEEECCCHHHHHHHHH
ELRALNNKGRLITIFGGSENRDAIARRVQMGIVASKLSDLVIVTDQDSGNEDPAAIRKEI
HHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
LQVARSAIEIPNRGEAIKYAISIMVDNDILLIAGKGHETRQVLKGRIINYNDMEQVKLYL
HHHHHHHHCCCCCCCEEEEEEEEEECCCEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHH
KQLNKMSNQ
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]