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The map label for this gene is dnaN [H]

Identifier: 189184479

GI number: 189184479

Start: 1599725

End: 1601023

Strand: Reverse

Name: dnaN [H]

Synonym: OTT_1572

Alternate gene names: 189184479

Gene position: 1601023-1599725 (Counterclockwise)

Preceding gene: 189184480

Following gene: 189184478

Centisome position: 79.69

GC content: 28.48

Gene sequence:

>1299_bases
ATGAATACTAAGTATCCAAAGGTTGATCATAATGATCTAGTAATATCTGACTTTAACATCAAGCTTGATACCAAAAATTT
TTCCCGCGCTCTCAATTTTTGCATGCCTATACTGGAAAAAAAAAATGTAGATCAAATTTTAAGTAATGTTAAGCTTACTC
ATATCAATGATACTGTAGAAAAGCGTTTGGAAATAAGCGCTACTGATGGAGATATTTTTATTTACCAAAAGCTACCAATT
AAAATTAGTAAAAATGATATTAGCATAACTCTTCCATTACAGGCATTAAATGAAATAGTGAAAAAAATTCCACATGATGA
ATTTACTATCTATTGTAACCAAGATAATAAAATGGTGCAAATTAAAGTAGGTGAATATCTTGAGTTTAATCTAGCAACAT
TGCCTATCAATCTTTTTCCAGTAATTGAAGAACTTACTGATGCAGATTTTATTATTCACGTCACTGCTATGCAGTTAGCG
CAGATGATTGAATATACTAAATTTGCTATATCTACAGAAGAAACACGATATAATCTCAATGGTATTTATCTACATTCAAA
AGACTATAAATCATTAATAGCAGTAGCAACTGACGGGCACCGGCTTGCAAGTGCTAAATGCAACATTGATACTTTTAAAA
CCAATATATCTAATAATGATGGCTTTGGTGTTATTTTGCCACGTAAAACTATAACAGAATTATATAAAATTTTTAAAGAA
CCATCAATAAATCAATATAACGTCATTATAAAAGTAAAAAGTAATAAAATACAATTTGATTGTCAAAATATTACTATTAT
TTCAAAATTAATAGATGGTAACTTTCCAGAATATGAACTGTTTATTCCTGCTAATAACACAAAAAAAATAATTCTTAATT
CTAATGATCTTTCTGAATCAATTGAAAGAGTTTCTACTATCGCAGTTGAAAAATCTAAAATAGTTATTTTAGATTTAGAT
CATCATGCTATTACAATTAGTGCTTACGGTAATGCTCAATGCAAGGCTAAAGAATTATTACTTCGACACCAGCATAATAA
CAAGAATGATCAACATCAATCTTCTAAACAGGAAGTAGAAAAATATGAATATATAGGCGATGATCAACTTACAATAGCAT
TTAATGCAAAATACTTGCTTGATGTTTTAAGTGTATGTAATAATATTAAAATTCCTATTGAATTTAAAGACTCTATATCT
CCAGCTTTGATTCAATTAGAAGAAAAAAACACTAGCGCCACTTTTATAATAATGCCAGTTAGTGTCCCTACTCAAGAGTT
TGTTAGTGCTCCTACTTAA

Upstream 100 bases:

>100_bases
TAAAACAGTAGAAGATCTAAGTAAACAGGAGCAAGAATTTGAAGATGAAGTACGTCTATTAATGAGAATACTGCAAAATT
AATATGAGTAATGAATAATT

Downstream 100 bases:

>100_bases
GAGTAAGATATTTATAATATAGTATTTTTACTATGCTATATTTAGATTGTTTATTTATGGGTTATTTTTTAATAATGCTA
ATATTTAGTATTAACTTGTT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MNTKYPKVDHNDLVISDFNIKLDTKNFSRALNFCMPILEKKNVDQILSNVKLTHINDTVEKRLEISATDGDIFIYQKLPI
KISKNDISITLPLQALNEIVKKIPHDEFTIYCNQDNKMVQIKVGEYLEFNLATLPINLFPVIEELTDADFIIHVTAMQLA
QMIEYTKFAISTEETRYNLNGIYLHSKDYKSLIAVATDGHRLASAKCNIDTFKTNISNNDGFGVILPRKTITELYKIFKE
PSINQYNVIIKVKSNKIQFDCQNITIISKLIDGNFPEYELFIPANNTKKIILNSNDLSESIERVSTIAVEKSKIVILDLD
HHAITISAYGNAQCKAKELLLRHQHNNKNDQHQSSKQEVEKYEYIGDDQLTIAFNAKYLLDVLSVCNNIKIPIEFKDSIS
PALIQLEEKNTSATFIIMPVSVPTQEFVSAPT

Sequences:

>Translated_432_residues
MNTKYPKVDHNDLVISDFNIKLDTKNFSRALNFCMPILEKKNVDQILSNVKLTHINDTVEKRLEISATDGDIFIYQKLPI
KISKNDISITLPLQALNEIVKKIPHDEFTIYCNQDNKMVQIKVGEYLEFNLATLPINLFPVIEELTDADFIIHVTAMQLA
QMIEYTKFAISTEETRYNLNGIYLHSKDYKSLIAVATDGHRLASAKCNIDTFKTNISNNDGFGVILPRKTITELYKIFKE
PSINQYNVIIKVKSNKIQFDCQNITIISKLIDGNFPEYELFIPANNTKKIILNSNDLSESIERVSTIAVEKSKIVILDLD
HHAITISAYGNAQCKAKELLLRHQHNNKNDQHQSSKQEVEKYEYIGDDQLTIAFNAKYLLDVLSVCNNIKIPIEFKDSIS
PALIQLEEKNTSATFIIMPVSVPTQEFVSAPT
>Mature_432_residues
MNTKYPKVDHNDLVISDFNIKLDTKNFSRALNFCMPILEKKNVDQILSNVKLTHINDTVEKRLEISATDGDIFIYQKLPI
KISKNDISITLPLQALNEIVKKIPHDEFTIYCNQDNKMVQIKVGEYLEFNLATLPINLFPVIEELTDADFIIHVTAMQLA
QMIEYTKFAISTEETRYNLNGIYLHSKDYKSLIAVATDGHRLASAKCNIDTFKTNISNNDGFGVILPRKTITELYKIFKE
PSINQYNVIIKVKSNKIQFDCQNITIISKLIDGNFPEYELFIPANNTKKIILNSNDLSESIERVSTIAVEKSKIVILDLD
HHAITISAYGNAQCKAKELLLRHQHNNKNDQHQSSKQEVEKYEYIGDDQLTIAFNAKYLLDVLSVCNNIKIPIEFKDSIS
PALIQLEEKNTSATFIIMPVSVPTQEFVSAPT

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=381, Percent_Identity=25.7217847769029, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 49193; Mature: 49193

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKYPKVDHNDLVISDFNIKLDTKNFSRALNFCMPILEKKNVDQILSNVKLTHINDTVE
CCCCCCCCCCCCEEEEEEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHCCEEEEECHHHH
KRLEISATDGDIFIYQKLPIKISKNDISITLPLQALNEIVKKIPHDEFTIYCNQDNKMVQ
CCEEEEECCCCEEEEEEECEEEECCCEEEEEEHHHHHHHHHHCCCCCEEEEECCCCCEEE
IKVGEYLEFNLATLPINLFPVIEELTDADFIIHVTAMQLAQMIEYTKFAISTEETRYNLN
EEECCEEEEEEEEECEEHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHEEECCCCEEEEE
GIYLHSKDYKSLIAVATDGHRLASAKCNIDTFKTNISNNDGFGVILPRKTITELYKIFKE
EEEEECCCCCEEEEEEECCCEEEEEECCCEEEEEECCCCCCEEEEECHHHHHHHHHHHCC
PSINQYNVIIKVKSNKIQFDCQNITIISKLIDGNFPEYELFIPANNTKKIILNSNDLSES
CCCCEEEEEEEEECCEEEEEECCEEEEEHHHCCCCCCEEEEEECCCCEEEEECCCCHHHH
IERVSTIAVEKSKIVILDLDHHAITISAYGNAQCKAKELLLRHQHNNKNDQHQSSKQEVE
HHHHHHEEEECCEEEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
KYEYIGDDQLTIAFNAKYLLDVLSVCNNIKIPIEFKDSISPALIQLEEKNTSATFIIMPV
HHHCCCCCEEEEEECHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCEEEEEEEC
SVPTQEFVSAPT
CCCHHHHHCCCC
>Mature Secondary Structure
MNTKYPKVDHNDLVISDFNIKLDTKNFSRALNFCMPILEKKNVDQILSNVKLTHINDTVE
CCCCCCCCCCCCEEEEEEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHCCEEEEECHHHH
KRLEISATDGDIFIYQKLPIKISKNDISITLPLQALNEIVKKIPHDEFTIYCNQDNKMVQ
CCEEEEECCCCEEEEEEECEEEECCCEEEEEEHHHHHHHHHHCCCCCEEEEECCCCCEEE
IKVGEYLEFNLATLPINLFPVIEELTDADFIIHVTAMQLAQMIEYTKFAISTEETRYNLN
EEECCEEEEEEEEECEEHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHEEECCCCEEEEE
GIYLHSKDYKSLIAVATDGHRLASAKCNIDTFKTNISNNDGFGVILPRKTITELYKIFKE
EEEEECCCCCEEEEEEECCCEEEEEECCCEEEEEECCCCCCEEEEECHHHHHHHHHHHCC
PSINQYNVIIKVKSNKIQFDCQNITIISKLIDGNFPEYELFIPANNTKKIILNSNDLSES
CCCCEEEEEEEEECCEEEEEECCEEEEEHHHCCCCCCEEEEEECCCCEEEEECCCCHHHH
IERVSTIAVEKSKIVILDLDHHAITISAYGNAQCKAKELLLRHQHNNKNDQHQSSKQEVE
HHHHHHEEEECCEEEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
KYEYIGDDQLTIAFNAKYLLDVLSVCNNIKIPIEFKDSISPALIQLEEKNTSATFIIMPV
HHHCCCCCEEEEEECHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCEEEEEEEC
SVPTQEFVSAPT
CCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]