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The map label for this gene is dnaA [H]

Identifier: 189184480

GI number: 189184480

Start: 1601042

End: 1602454

Strand: Reverse

Name: dnaA [H]

Synonym: OTT_1573

Alternate gene names: 189184480

Gene position: 1602454-1601042 (Counterclockwise)

Preceding gene: 189184483

Following gene: 189184479

Centisome position: 79.76

GC content: 33.47

Gene sequence:

>1413_bases
ATGAATACACTTCTGCAACAGCAAAATACTACTAAAGATGGGTTATACTTTCATGAGTTATGGAGTAATGTTAAGCGAGA
TTTAAGGCATCATTATGGTGAAGCTGTATTTAAAAGTTGGTTTAGTAAAATGAATATGGTTGATTTTACAGATGGATATA
TAAAGCTGTCTGCTCCTACTAATTTTACGCGCGATTGGATAAAGGCCAACTACTTTGATTCTATTAGGCAGCTATTAGCA
TATTATGATAGTTCTATTACAAGCATTGATATTGTTACTTCTGCTATTGACCATCAAAATTCTGATTTGGCATTTTCTAC
TCAACAAAATACAGATAATAATAATAATCAGCAATATAGTACTAAAAGCAGCAATAATGTTATTTTTTCCTCAACGCTAG
ATCCAAGATTTACTTTTGATAATTTTGTAGTTGGTAGGCCAAATGAGTTAGCATATGCAGCAGCAACTACAGTAGCTGAA
TCGCCAACTGCTGTTGCAAAATCTAACCCTCTTTTTTTATATGGCGGAGTTGGTCTTGGAAAAACACATCTAATGCATGC
AATAGCATGGCATATTCAGAACAGTAGTTCTAAACGTAAAGTAATTTATATGTCAGCTGAAAAATTTATGTATCAGTTTG
TTCAATCTTTAAGAAATAAAGATGTTATGTCATTTAAAGAACAATTCAGGTCAGTAGATGTGCTTATGATTGATGATATT
CAATTTATTTGTGGTAAAGATAGTACTCAAGAGGAGTTTTTTCACACATTTAACGCACTAATCGATAATAAGCGACAAAT
GGTGATTTCATGTGATCGATCTCCATCTGACCTGGACAATATTGAAGAAAGAATTAAATCTAGATTAGGATGGGGATTAG
TGGCAGACGTCCATAGTACAACTTATGAGCTAAGATTAGGCATACTGCAGTCCAAAGTGGAACAGCTGGCTACAGCAGTT
CCGCTAAAAGTATTAGAGTTTTTAGCTGCTAAAATTACTTCAAATGTACGCGAACTAGAAGGAGCTTTAAACAAAGTGAT
AGCCCATTCCACTCTAGTTGGATATGAAATTACTATTGAAAATACTCAAGAAATTTTAAGAGATTTGCTACGATCTAACC
AGAAAACAATTACCATTGATAGCATTCAAAAAAAAGTAGCTGAGCGTTATAATATTAAGATATCTGATATGTCATCTAGT
AGAAGATCAAGATCAGTAACTAGGCCACGTCAGCTTGCAATGTATCTTGCAAAACGGCTGACATCTAAGAGTTTAGTAGA
TATTGGAAAAGCATTTGGCAAAAAAGATCATACCACAGTCATGCATGCTATTAAAACAGTAGAAGATCTAAGTAAACAGG
AGCAAGAATTTGAAGATGAAGTACGTCTATTAATGAGAATACTGCAAAATTAA

Upstream 100 bases:

>100_bases
ATATTGTTTACAGCATATATGCTTTAATTATATGCAACATTTAGTTGATAATTTATTTTAATCTTAATACCAACCATTGT
AACTGATCTTAATAAATGTA

Downstream 100 bases:

>100_bases
TATGAGTAATGAATAATTATGAATACTAAGTATCCAAAGGTTGATCATAATGATCTAGTAATATCTGACTTTAACATCAA
GCTTGATACCAAAAATTTTT

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MNTLLQQQNTTKDGLYFHELWSNVKRDLRHHYGEAVFKSWFSKMNMVDFTDGYIKLSAPTNFTRDWIKANYFDSIRQLLA
YYDSSITSIDIVTSAIDHQNSDLAFSTQQNTDNNNNQQYSTKSSNNVIFSSTLDPRFTFDNFVVGRPNELAYAAATTVAE
SPTAVAKSNPLFLYGGVGLGKTHLMHAIAWHIQNSSSKRKVIYMSAEKFMYQFVQSLRNKDVMSFKEQFRSVDVLMIDDI
QFICGKDSTQEEFFHTFNALIDNKRQMVISCDRSPSDLDNIEERIKSRLGWGLVADVHSTTYELRLGILQSKVEQLATAV
PLKVLEFLAAKITSNVRELEGALNKVIAHSTLVGYEITIENTQEILRDLLRSNQKTITIDSIQKKVAERYNIKISDMSSS
RRSRSVTRPRQLAMYLAKRLTSKSLVDIGKAFGKKDHTTVMHAIKTVEDLSKQEQEFEDEVRLLMRILQN

Sequences:

>Translated_470_residues
MNTLLQQQNTTKDGLYFHELWSNVKRDLRHHYGEAVFKSWFSKMNMVDFTDGYIKLSAPTNFTRDWIKANYFDSIRQLLA
YYDSSITSIDIVTSAIDHQNSDLAFSTQQNTDNNNNQQYSTKSSNNVIFSSTLDPRFTFDNFVVGRPNELAYAAATTVAE
SPTAVAKSNPLFLYGGVGLGKTHLMHAIAWHIQNSSSKRKVIYMSAEKFMYQFVQSLRNKDVMSFKEQFRSVDVLMIDDI
QFICGKDSTQEEFFHTFNALIDNKRQMVISCDRSPSDLDNIEERIKSRLGWGLVADVHSTTYELRLGILQSKVEQLATAV
PLKVLEFLAAKITSNVRELEGALNKVIAHSTLVGYEITIENTQEILRDLLRSNQKTITIDSIQKKVAERYNIKISDMSSS
RRSRSVTRPRQLAMYLAKRLTSKSLVDIGKAFGKKDHTTVMHAIKTVEDLSKQEQEFEDEVRLLMRILQN
>Mature_470_residues
MNTLLQQQNTTKDGLYFHELWSNVKRDLRHHYGEAVFKSWFSKMNMVDFTDGYIKLSAPTNFTRDWIKANYFDSIRQLLA
YYDSSITSIDIVTSAIDHQNSDLAFSTQQNTDNNNNQQYSTKSSNNVIFSSTLDPRFTFDNFVVGRPNELAYAAATTVAE
SPTAVAKSNPLFLYGGVGLGKTHLMHAIAWHIQNSSSKRKVIYMSAEKFMYQFVQSLRNKDVMSFKEQFRSVDVLMIDDI
QFICGKDSTQEEFFHTFNALIDNKRQMVISCDRSPSDLDNIEERIKSRLGWGLVADVHSTTYELRLGILQSKVEQLATAV
PLKVLEFLAAKITSNVRELEGALNKVIAHSTLVGYEITIENTQEILRDLLRSNQKTITIDSIQKKVAERYNIKISDMSSS
RRSRSVTRPRQLAMYLAKRLTSKSLVDIGKAFGKKDHTTVMHAIKTVEDLSKQEQEFEDEVRLLMRILQN

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=464, Percent_Identity=41.3793103448276, Blast_Score=367, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 53806; Mature: 53806

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLLQQQNTTKDGLYFHELWSNVKRDLRHHYGEAVFKSWFSKMNMVDFTDGYIKLSAPT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCC
NFTRDWIKANYFDSIRQLLAYYDSSITSIDIVTSAIDHQNSDLAFSTQQNTDNNNNQQYS
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEE
TKSSNNVIFSSTLDPRFTFDNFVVGRPNELAYAAATTVAESPTAVAKSNPLFLYGGVGLG
CCCCCCEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCCHHCCCCCEEEECCCCCC
KTHLMHAIAWHIQNSSSKRKVIYMSAEKFMYQFVQSLRNKDVMSFKEQFRSVDVLMIDDI
HHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCC
QFICGKDSTQEEFFHTFNALIDNKRQMVISCDRSPSDLDNIEERIKSRLGWGLVADVHST
HHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEHHHHH
TYELRLGILQSKVEQLATAVPLKVLEFLAAKITSNVRELEGALNKVIAHSTLVGYEITIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEC
NTQEILRDLLRSNQKTITIDSIQKKVAERYNIKISDMSSSRRSRSVTRPRQLAMYLAKRL
CHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCEEEECCCCHHHHHCCCCHHHHHHHHHHHH
TSKSLVDIGKAFGKKDHTTVMHAIKTVEDLSKQEQEFEDEVRLLMRILQN
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTLLQQQNTTKDGLYFHELWSNVKRDLRHHYGEAVFKSWFSKMNMVDFTDGYIKLSAPT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCC
NFTRDWIKANYFDSIRQLLAYYDSSITSIDIVTSAIDHQNSDLAFSTQQNTDNNNNQQYS
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEE
TKSSNNVIFSSTLDPRFTFDNFVVGRPNELAYAAATTVAESPTAVAKSNPLFLYGGVGLG
CCCCCCEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCCHHCCCCCEEEECCCCCC
KTHLMHAIAWHIQNSSSKRKVIYMSAEKFMYQFVQSLRNKDVMSFKEQFRSVDVLMIDDI
HHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCC
QFICGKDSTQEEFFHTFNALIDNKRQMVISCDRSPSDLDNIEERIKSRLGWGLVADVHST
HHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEHHHHH
TYELRLGILQSKVEQLATAVPLKVLEFLAAKITSNVRELEGALNKVIAHSTLVGYEITIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEC
NTQEILRDLLRSNQKTITIDSIQKKVAERYNIKISDMSSSRRSRSVTRPRQLAMYLAKRL
CHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCEEEECCCCHHHHHCCCCHHHHHHHHHHHH
TSKSLVDIGKAFGKKDHTTVMHAIKTVEDLSKQEQEFEDEVRLLMRILQN
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA