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The map label for this gene is pcnB [H]

Identifier: 189025823

GI number: 189025823

Start: 649042

End: 650154

Strand: Reverse

Name: pcnB [H]

Synonym: TPASS_0596

Alternate gene names: 189025823

Gene position: 650154-649042 (Counterclockwise)

Preceding gene: 189025824

Following gene: 189025822

Centisome position: 57.06

GC content: 56.33

Gene sequence:

>1113_bases
ATGTTGGTTCGCTACAGTTACGATGCAAAGGGAAGGCGGTTGGGGCGTGCGCTGGTGTACACTGAGTCGGAGCACGGTAT
ACCTCGGCAGAGCGTTGACGCTGGGGCGATAAGGGTTGTAGAAGCGCTGGTGGGTGCGGGGTATGAAACCTATATCGTCG
GTGGGGCGGTAAGGGACCTGGTTGCGGGAAGGACACCAAAAGATTTTGACATTGTTACAGGCGCAGTTCCCTCTAGGATT
CGTAGGTTGTTCAGGAACTCGCGCATTATCGGCAGGCGCTTCCGCATTGTTCATGTGTCGTGTGGCTCGCAGCTGTACGA
GGTTTCCACCTTTCGCTCTCGTGTGGGGGAAGGTTCGGTGTGTGTTCCTGGCACGTTGGAGGAAGATGCATGGCGGAGGG
ACTTTAGTGTCAATGCCTTGTACTATGATCCTCTGAGAAATGTGGTGATCGATTGTGTCGGTGGAATGGTTGATCTGAAG
AGGCGTCGCGTGCGGCCGCTCATACCTCTGCGGTCCATCTTTGTAGAGGACCCAGTGCGCATGCTCCGGGCATTGAAGTG
CTCGGTGATGTGCGAGTCTTCCATCCCTTTTTCTGTCCGCCGCAGTATTCGCCGCAGTGTTTCCCTTCTTGGGGGGTGCT
CTCCCTCCCGGTTGACCGACGAATTTGTAAAAATCCTCTTTTCCGGTCGGAGCGCCGCGCTTGTGCGCGCCCTATGTGGG
TACCAGCTCCTTCTGTACTTGCAGCCGTCTGTGCACTACTTTATGCGGGATGTGCCGTGCTTTGAGAGCGCTTTTTTTGC
AAGCTTGAGGGTGCTAGATCAGGCTTTTGTGTCTGGGAGGGTGAAGCGTGTGGGGCAAGCGCTTACGTACGTGCTGCGCG
ATTTTGTCGGCCTGGTGTTTGGTCATGCAGACGCACCGGATGAGGTGTACATAAGGGTGTATCGAGCATGTCGCAGTTTT
GTGTTGCCAATGAATCCTCCCCGTGTAGAATTGGAGTACGCGGTGCGCTCTTGTTTGGGCGGTCGGGGTTTCGCGGCGCG
CGCGGCGTGTACGCCTTCGGGTCGGTTGTGTGCGCGAGAGAACGGGGTGAGGTGTGTTTTTTCTTCCCCGTAG

Upstream 100 bases:

>100_bases
CACAAGGTGCGGTGTATTTTGTAATTCGGCACGGTGGTATGAATGCTTCCTAGTTGGTCTTGACAGGGAGCTCCTTCTCG
GGGGAGGATGGGCGGGGTAG

Downstream 100 bases:

>100_bases
GAAGATAAGGTGGAGTTTGTTTCATGAGGTTTGTTTTTCTTGGTCCTCCTGGTGCCGGTAAGGGTACGCTTGCAGGTGAA
ATTTCTGGGCGTTGTGGCGT

Product: polynucleotide adenylyltransferase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MLVRYSYDAKGRRLGRALVYTESEHGIPRQSVDAGAIRVVEALVGAGYETYIVGGAVRDLVAGRTPKDFDIVTGAVPSRI
RRLFRNSRIIGRRFRIVHVSCGSQLYEVSTFRSRVGEGSVCVPGTLEEDAWRRDFSVNALYYDPLRNVVIDCVGGMVDLK
RRRVRPLIPLRSIFVEDPVRMLRALKCSVMCESSIPFSVRRSIRRSVSLLGGCSPSRLTDEFVKILFSGRSAALVRALCG
YQLLLYLQPSVHYFMRDVPCFESAFFASLRVLDQAFVSGRVKRVGQALTYVLRDFVGLVFGHADAPDEVYIRVYRACRSF
VLPMNPPRVELEYAVRSCLGGRGFAARAACTPSGRLCARENGVRCVFSSP

Sequences:

>Translated_370_residues
MLVRYSYDAKGRRLGRALVYTESEHGIPRQSVDAGAIRVVEALVGAGYETYIVGGAVRDLVAGRTPKDFDIVTGAVPSRI
RRLFRNSRIIGRRFRIVHVSCGSQLYEVSTFRSRVGEGSVCVPGTLEEDAWRRDFSVNALYYDPLRNVVIDCVGGMVDLK
RRRVRPLIPLRSIFVEDPVRMLRALKCSVMCESSIPFSVRRSIRRSVSLLGGCSPSRLTDEFVKILFSGRSAALVRALCG
YQLLLYLQPSVHYFMRDVPCFESAFFASLRVLDQAFVSGRVKRVGQALTYVLRDFVGLVFGHADAPDEVYIRVYRACRSF
VLPMNPPRVELEYAVRSCLGGRGFAARAACTPSGRLCARENGVRCVFSSP
>Mature_370_residues
MLVRYSYDAKGRRLGRALVYTESEHGIPRQSVDAGAIRVVEALVGAGYETYIVGGAVRDLVAGRTPKDFDIVTGAVPSRI
RRLFRNSRIIGRRFRIVHVSCGSQLYEVSTFRSRVGEGSVCVPGTLEEDAWRRDFSVNALYYDPLRNVVIDCVGGMVDLK
RRRVRPLIPLRSIFVEDPVRMLRALKCSVMCESSIPFSVRRSIRRSVSLLGGCSPSRLTDEFVKILFSGRSAALVRALCG
YQLLLYLQPSVHYFMRDVPCFESAFFASLRVLDQAFVSGRVKRVGQALTYVLRDFVGLVFGHADAPDEVYIRVYRACRSF
VLPMNPPRVELEYAVRSCLGGRGFAARAACTPSGRLCARENGVRCVFSSP

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=253, Percent_Identity=39.5256916996047, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1789436, Length=140, Percent_Identity=35, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 41317; Mature: 41317

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVRYSYDAKGRRLGRALVYTESEHGIPRQSVDAGAIRVVEALVGAGYETYIVGGAVRDL
CEEEECCCCCCHHHCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECHHHHHH
VAGRTPKDFDIVTGAVPSRIRRLFRNSRIIGRRFRIVHVSCGSQLYEVSTFRSRVGEGSV
HCCCCCCCCHHCCCHHHHHHHHHHHCCCEECCEEEEEEEECCCHHHHHHHHHHHCCCCCE
CVPGTLEEDAWRRDFSVNALYYDPLRNVVIDCVGGMVDLKRRRVRPLIPLRSIFVEDPVR
ECCCCCCHHHHHCCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHH
MLRALKCSVMCESSIPFSVRRSIRRSVSLLGGCSPSRLTDEFVKILFSGRSAALVRALCG
HHHHHHHHHEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
YQLLLYLQPSVHYFMRDVPCFESAFFASLRVLDQAFVSGRVKRVGQALTYVLRDFVGLVF
HHEEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GHADAPDEVYIRVYRACRSFVLPMNPPRVELEYAVRSCLGGRGFAARAACTPSGRLCARE
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCEECCCCCCEEECC
NGVRCVFSSP
CCCEEEECCC
>Mature Secondary Structure
MLVRYSYDAKGRRLGRALVYTESEHGIPRQSVDAGAIRVVEALVGAGYETYIVGGAVRDL
CEEEECCCCCCHHHCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECHHHHHH
VAGRTPKDFDIVTGAVPSRIRRLFRNSRIIGRRFRIVHVSCGSQLYEVSTFRSRVGEGSV
HCCCCCCCCHHCCCHHHHHHHHHHHCCCEECCEEEEEEEECCCHHHHHHHHHHHCCCCCE
CVPGTLEEDAWRRDFSVNALYYDPLRNVVIDCVGGMVDLKRRRVRPLIPLRSIFVEDPVR
ECCCCCCHHHHHCCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHH
MLRALKCSVMCESSIPFSVRRSIRRSVSLLGGCSPSRLTDEFVKILFSGRSAALVRALCG
HHHHHHHHHEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
YQLLLYLQPSVHYFMRDVPCFESAFFASLRVLDQAFVSGRVKRVGQALTYVLRDFVGLVF
HHEEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GHADAPDEVYIRVYRACRSFVLPMNPPRVELEYAVRSCLGGRGFAARAACTPSGRLCARE
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCEECCCCCCEEECC
NGVRCVFSSP
CCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]