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The map label for this gene is thlA [H]

Identifier: 188586522

GI number: 188586522

Start: 2002785

End: 2003963

Strand: Reverse

Name: thlA [H]

Synonym: Nther_1908

Alternate gene names: 188586522

Gene position: 2003963-2002785 (Counterclockwise)

Preceding gene: 188586523

Following gene: 188586521

Centisome position: 63.31

GC content: 43.94

Gene sequence:

>1179_bases
GTGGAAAAGATAGTAATCGCTTCTGGAGTAAGGACTCCTATTGGAACATATGGAGGTTCTCTTAAGGACTTTCCTGCTCA
CAAGCTTGGTGAAAAAGTGATTAAAGAAGCTGTGAACAGAGCTTCCATAGCTCCAGAGGACGTAGATGAAGTAATAATGG
GCAATGTATTACAGGCAGGTCAAGGAATGAATCCATCACGTCAAGCATCCTTAGGTGCAGGAATTCCCAAGGAAACGCCA
GCGTTGACTATTAATAAAGTATGCGGCTCTGGTTTGAAAGCTTTAATATTAGGAGCACAAGCCATCAAAGACGGCGACAG
TGAAGTGGTTGTCGTGGGCGGTATGGAAAACATGAGTCAGGCACCATATTATTTGTCCAAAGCGAGATTCGGATATGGTA
TGGGGCATGGCAAAGTGGAAGATGCTATGATTAAAGACGGCCTATGGTGTTCAATCACTGATCAGCACATGGGGAATACG
GCTGAAGCCATAGCCGAACATTATAATATAACCCGAGAAGAACAGGACGAATTTAGTGTGGACAGCCAAAATAAGGCAGA
AAAAGCCATTTCCGAAGGCTGGTTTCAAGAGGAGATTCTGCCAATAGAAATTCCACAGAAAAAGAAAGATCCAATTATTT
TCGATACTGATGAACATCCCAAGCCTGGGACCACATTAGACAAATTAGCTAAAATGAAGCCTGCATTCCAAAAAGAAGGT
ACAGTAACTGCAGCTAATGCTTCTGGTATAAATGATGGGGCTGCTGCCATGGTGATCACAACTGAATCCAAAGCCAAAGA
ATTAGGCCTTGAGCCCTTGGGAGTATTAGATGGTTACGCTTCTGCCGGTTGTGAACCTGAGCATATGGGAATAGGACCAG
TACCAGCTACTAGAAAAGTCTTAGATAAAACTGGCTTAAGCGTAGATGATCTAGATCTGATTGAAGCTAATGAAGCCTTT
GCCGTACAAAGTGTTGCTGTCATGAAAGAGCTGGGCCTCGATGAAGAACGAGTGAACATTGGAGGAGGAGCAGTCTCTCT
AGGTCACCCCATCGGAGCCAGTGGTGCTCGAATTTTAATCACTCTGTTATATCACATGAAGCGAACAGACAAAAAACGAG
GATTAGCCACCCTTTGCGTCGGCGGTGGAATGGGTGTTTCAACTATAGTATCTAGATAA

Upstream 100 bases:

>100_bases
AACAATCTAGTATTGAAGAGGTGCAAGATAAAACTGAAGCGGACCTAATTATCCCTGATAACGTAGGGGTTTTAAAATAA
CAAGATTGGGAGGGTTAATA

Downstream 100 bases:

>100_bases
AGTATGGGCTTGATTAATAGTAAATGAAAAAGTTTCCATGTTAAAAGAAAGTAAGTAATCCTATATTGAAAGACGGGGAA
TATCAAGTACCCGTCTTTTC

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MEKIVIASGVRTPIGTYGGSLKDFPAHKLGEKVIKEAVNRASIAPEDVDEVIMGNVLQAGQGMNPSRQASLGAGIPKETP
ALTINKVCGSGLKALILGAQAIKDGDSEVVVVGGMENMSQAPYYLSKARFGYGMGHGKVEDAMIKDGLWCSITDQHMGNT
AEAIAEHYNITREEQDEFSVDSQNKAEKAISEGWFQEEILPIEIPQKKKDPIIFDTDEHPKPGTTLDKLAKMKPAFQKEG
TVTAANASGINDGAAAMVITTESKAKELGLEPLGVLDGYASAGCEPEHMGIGPVPATRKVLDKTGLSVDDLDLIEANEAF
AVQSVAVMKELGLDEERVNIGGGAVSLGHPIGASGARILITLLYHMKRTDKKRGLATLCVGGGMGVSTIVSR

Sequences:

>Translated_392_residues
MEKIVIASGVRTPIGTYGGSLKDFPAHKLGEKVIKEAVNRASIAPEDVDEVIMGNVLQAGQGMNPSRQASLGAGIPKETP
ALTINKVCGSGLKALILGAQAIKDGDSEVVVVGGMENMSQAPYYLSKARFGYGMGHGKVEDAMIKDGLWCSITDQHMGNT
AEAIAEHYNITREEQDEFSVDSQNKAEKAISEGWFQEEILPIEIPQKKKDPIIFDTDEHPKPGTTLDKLAKMKPAFQKEG
TVTAANASGINDGAAAMVITTESKAKELGLEPLGVLDGYASAGCEPEHMGIGPVPATRKVLDKTGLSVDDLDLIEANEAF
AVQSVAVMKELGLDEERVNIGGGAVSLGHPIGASGARILITLLYHMKRTDKKRGLATLCVGGGMGVSTIVSR
>Mature_392_residues
MEKIVIASGVRTPIGTYGGSLKDFPAHKLGEKVIKEAVNRASIAPEDVDEVIMGNVLQAGQGMNPSRQASLGAGIPKETP
ALTINKVCGSGLKALILGAQAIKDGDSEVVVVGGMENMSQAPYYLSKARFGYGMGHGKVEDAMIKDGLWCSITDQHMGNT
AEAIAEHYNITREEQDEFSVDSQNKAEKAISEGWFQEEILPIEIPQKKKDPIIFDTDEHPKPGTTLDKLAKMKPAFQKEG
TVTAANASGINDGAAAMVITTESKAKELGLEPLGVLDGYASAGCEPEHMGIGPVPATRKVLDKTGLSVDDLDLIEANEAF
AVQSVAVMKELGLDEERVNIGGGAVSLGHPIGASGARILITLLYHMKRTDKKRGLATLCVGGGMGVSTIVSR

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=393, Percent_Identity=49.618320610687, Blast_Score=387, Evalue=1e-108,
Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=45.5696202531646, Blast_Score=358, Evalue=3e-99,
Organism=Homo sapiens, GI4557237, Length=393, Percent_Identity=44.2748091603053, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI4501853, Length=392, Percent_Identity=40.3061224489796, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=33.1764705882353, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI194353979, Length=392, Percent_Identity=29.8469387755102, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1788554, Length=393, Percent_Identity=55.7251908396947, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI87082165, Length=392, Percent_Identity=46.9387755102041, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=42.3940149625935, Blast_Score=315, Evalue=4e-87,
Organism=Escherichia coli, GI48994986, Length=403, Percent_Identity=39.454094292804, Blast_Score=271, Evalue=5e-74,
Organism=Escherichia coli, GI1788683, Length=413, Percent_Identity=32.6876513317191, Blast_Score=214, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI133906874, Length=394, Percent_Identity=43.6548223350254, Blast_Score=332, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI17535921, Length=393, Percent_Identity=41.4758269720102, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI25147385, Length=378, Percent_Identity=39.9470899470899, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17551802, Length=425, Percent_Identity=31.0588235294118, Blast_Score=198, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17535917, Length=392, Percent_Identity=31.1224489795918, Blast_Score=163, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6325229, Length=393, Percent_Identity=42.7480916030534, Blast_Score=293, Evalue=3e-80,
Organism=Saccharomyces cerevisiae, GI6322031, Length=397, Percent_Identity=38.0352644836272, Blast_Score=243, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24655093, Length=392, Percent_Identity=48.469387755102, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17648125, Length=393, Percent_Identity=43.0025445292621, Blast_Score=314, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24640423, Length=391, Percent_Identity=42.1994884910486, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=31.6901408450704, Blast_Score=202, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41407; Mature: 41407

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIVIASGVRTPIGTYGGSLKDFPAHKLGEKVIKEAVNRASIAPEDVDEVIMGNVLQAG
CCCEEEECCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
QGMNPSRQASLGAGIPKETPALTINKVCGSGLKALILGAQAIKDGDSEVVVVGGMENMSQ
CCCCCHHHHHHCCCCCCCCCCEEHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCC
APYYLSKARFGYGMGHGKVEDAMIKDGLWCSITDQHMGNTAEAIAEHYNITREEQDEFSV
CCHHHHHHHCCCCCCCCCHHHHHHCCCCEEEECHHHCCCHHHHHHHHHCCCCCCCHHCCC
DSQNKAEKAISEGWFQEEILPIEIPQKKKDPIIFDTDEHPKPGTTLDKLAKMKPAFQKEG
CCCHHHHHHHHHCCHHHCCEEEECCCCCCCCEEECCCCCCCCCCHHHHHHHHCCCHHCCC
TVTAANASGINDGAAAMVITTESKAKELGLEPLGVLDGYASAGCEPEHMGIGPVPATRKV
CEEECCCCCCCCCCEEEEEEECHHHHHCCCCCHHHHCCHHHCCCCCCCCCCCCCCHHHHH
LDKTGLSVDDLDLIEANEAFAVQSVAVMKELGLDEERVNIGGGAVSLGHPIGASGARILI
HHHCCCCCCCCHHEECCCHHHHHHHHHHHHHCCCHHEEECCCCEEECCCCCCCCHHHHHH
TLLYHMKRTDKKRGLATLCVGGGMGVSTIVSR
HHHHHHHHHHHHCCEEEEEECCCCCHHHHHCC
>Mature Secondary Structure
MEKIVIASGVRTPIGTYGGSLKDFPAHKLGEKVIKEAVNRASIAPEDVDEVIMGNVLQAG
CCCEEEECCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
QGMNPSRQASLGAGIPKETPALTINKVCGSGLKALILGAQAIKDGDSEVVVVGGMENMSQ
CCCCCHHHHHHCCCCCCCCCCEEHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCC
APYYLSKARFGYGMGHGKVEDAMIKDGLWCSITDQHMGNTAEAIAEHYNITREEQDEFSV
CCHHHHHHHCCCCCCCCCHHHHHHCCCCEEEECHHHCCCHHHHHHHHHCCCCCCCHHCCC
DSQNKAEKAISEGWFQEEILPIEIPQKKKDPIIFDTDEHPKPGTTLDKLAKMKPAFQKEG
CCCHHHHHHHHHCCHHHCCEEEECCCCCCCCEEECCCCCCCCCCHHHHHHHHCCCHHCCC
TVTAANASGINDGAAAMVITTESKAKELGLEPLGVLDGYASAGCEPEHMGIGPVPATRKV
CEEECCCCCCCCCCEEEEEEECHHHHHCCCCCHHHHCCHHHCCCCCCCCCCCCCCHHHHH
LDKTGLSVDDLDLIEANEAFAVQSVAVMKELGLDEERVNIGGGAVSLGHPIGASGARILI
HHHCCCCCCCCHHEECCCHHHHHHHHHHHHHCCCHHEEECCCCEEECCCCCCCCHHHHHH
TLLYHMKRTDKKRGLATLCVGGGMGVSTIVSR
HHHHHHHHHHHHCCEEEEEECCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]