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The map label for this gene is ctfB [H]

Identifier: 188586523

GI number: 188586523

Start: 2003984

End: 2004643

Strand: Reverse

Name: ctfB [H]

Synonym: Nther_1909

Alternate gene names: 188586523

Gene position: 2004643-2003984 (Counterclockwise)

Preceding gene: 188586524

Following gene: 188586522

Centisome position: 63.33

GC content: 39.09

Gene sequence:

>660_bases
ATGGATAAAAAAGAAAAAAGAAATTTAATAGCTAAGCGTATAGCTCAAGAAATACAACCAGGAGAAGTGGTTAACCTAGG
AATAGGAATGCCTACCTTAGTAGCCAATTTTATTGAAGATGGTGAAAATATTTTAATCCAATCGGAAAATGGTGTCCTAG
GGGTCGGACCAGCTCCCGAAGAAGGTCAAGAAGACCCTGATTTAACAAATGCCGGTGGTCAACCAGTAACTATAATTCCT
GGTGGAGTTGGCTTCGATAGTGCAGAATCTTTTAGTATAATTAGAGGTGGACATCTGGATAGCACTGTTTTAGGAGCTTT
AGAAGTTGACCAAGAAGGAAATTTAGCTAATTGGATGATTCCTGGGAAACTAGTCCCTGGAATGGGAGGTGCCATGGACT
TAGTTGTCGGTGCCAAAAAGGTTATTATTGCAACTGAACATGTAAACAAAAACGGTGCCCCTAAAATTTTAAAAAAATGT
ACCTTACCCTTAACAGCCGAAAAAGAGGTCGATATGATAGTGACAGAACTATGTGTGATTGAAATTACTGAAGAAGGGGC
TGTGTTACGGGAAGTAGCTGAACAATCTAGTATTGAAGAGGTGCAAGATAAAACTGAAGCGGACCTAATTATCCCTGATA
ACGTAGGGGTTTTAAAATAA

Upstream 100 bases:

>100_bases
AAGTTGGAGAAATAGTAGAACCAGGTGAATTAGATCCAGACGAAGTTATAACTCCCGGTGTCTATGTTGACTATCTTGTG
AGAAGGGAGGAGGGATAACT

Downstream 100 bases:

>100_bases
CAAGATTGGGAGGGTTAATAGTGGAAAAGATAGTAATCGCTTCTGGAGTAAGGACTCCTATTGGAACATATGGAGGTTCT
CTTAAGGACTTTCCTGCTCA

Product: butyryl-CoA:acetate CoA transferase

Products: NA

Alternate protein names: Coat B; Acetoacetyl-CoA:acetate/butyrate:CoA transferase subunit B [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MDKKEKRNLIAKRIAQEIQPGEVVNLGIGMPTLVANFIEDGENILIQSENGVLGVGPAPEEGQEDPDLTNAGGQPVTIIP
GGVGFDSAESFSIIRGGHLDSTVLGALEVDQEGNLANWMIPGKLVPGMGGAMDLVVGAKKVIIATEHVNKNGAPKILKKC
TLPLTAEKEVDMIVTELCVIEITEEGAVLREVAEQSSIEEVQDKTEADLIIPDNVGVLK

Sequences:

>Translated_219_residues
MDKKEKRNLIAKRIAQEIQPGEVVNLGIGMPTLVANFIEDGENILIQSENGVLGVGPAPEEGQEDPDLTNAGGQPVTIIP
GGVGFDSAESFSIIRGGHLDSTVLGALEVDQEGNLANWMIPGKLVPGMGGAMDLVVGAKKVIIATEHVNKNGAPKILKKC
TLPLTAEKEVDMIVTELCVIEITEEGAVLREVAEQSSIEEVQDKTEADLIIPDNVGVLK
>Mature_219_residues
MDKKEKRNLIAKRIAQEIQPGEVVNLGIGMPTLVANFIEDGENILIQSENGVLGVGPAPEEGQEDPDLTNAGGQPVTIIP
GGVGFDSAESFSIIRGGHLDSTVLGALEVDQEGNLANWMIPGKLVPGMGGAMDLVVGAKKVIIATEHVNKNGAPKILKKC
TLPLTAEKEVDMIVTELCVIEITEEGAVLREVAEQSSIEEVQDKTEADLIIPDNVGVLK

Specific function: Involved in solventogenic switch which allows C.acetobutylicum to uptake acid and produce solvents. Acts mainly to detoxify the medium by removing the acetate and butyrate excreted earlier in the fermentation [H]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=207, Percent_Identity=50.7246376811594, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI11545841, Length=215, Percent_Identity=45.1162790697674, Blast_Score=201, Evalue=5e-52,
Organism=Escherichia coli, GI1788552, Length=209, Percent_Identity=55.0239234449761, Blast_Score=244, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI17531517, Length=213, Percent_Identity=48.8262910798122, Blast_Score=205, Evalue=1e-53,
Organism=Drosophila melanogaster, GI21358325, Length=211, Percent_Identity=50.2369668246445, Blast_Score=213, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24655794, Length=211, Percent_Identity=50.2369668246445, Blast_Score=213, Evalue=1e-55,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.9 [H]

Molecular weight: Translated: 23150; Mature: 23150

Theoretical pI: Translated: 4.14; Mature: 4.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKEKRNLIAKRIAQEIQPGEVVNLGIGMPTLVANFIEDGENILIQSENGVLGVGPAPE
CCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCEEEEECCCCEEECCCCCC
EGQEDPDLTNAGGQPVTIIPGGVGFDSAESFSIIRGGHLDSTVLGALEVDQEGNLANWMI
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEEE
PGKLVPGMGGAMDLVVGAKKVIIATEHVNKNGAPKILKKCTLPLTAEKEVDMIVTELCVI
CCEECCCCCCHHHHHCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHHEEEE
EITEEGAVLREVAEQSSIEEVQDKTEADLIIPDNVGVLK
EECCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCC
>Mature Secondary Structure
MDKKEKRNLIAKRIAQEIQPGEVVNLGIGMPTLVANFIEDGENILIQSENGVLGVGPAPE
CCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCEEEEECCCCEEECCCCCC
EGQEDPDLTNAGGQPVTIIPGGVGFDSAESFSIIRGGHLDSTVLGALEVDQEGNLANWMI
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEEE
PGKLVPGMGGAMDLVVGAKKVIIATEHVNKNGAPKILKKCTLPLTAEKEVDMIVTELCVI
CCEECCCCCCHHHHHCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHHEEEE
EITEEGAVLREVAEQSSIEEVQDKTEADLIIPDNVGVLK
EECCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254264; 8226639; 8423010; 11466286; 2383002 [H]