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The map label for this gene is rutB [H]

Identifier: 188586443

GI number: 188586443

Start: 1902133

End: 1902819

Strand: Reverse

Name: rutB [H]

Synonym: Nther_1828

Alternate gene names: 188586443

Gene position: 1902819-1902133 (Counterclockwise)

Preceding gene: 188586444

Following gene: 188586442

Centisome position: 60.11

GC content: 33.77

Gene sequence:

>687_bases
ATGGATCTAAATAAATCAGCTCTTTGTTTAATAGATATCCAAAAAGAAAGTAAGTTTGGAATTGAAAACATAGACAGTGT
TGTGGAAAATTCAAAACACTTAATTGAACAATGCCGCAAGTTAAATATACCCGTTATCTATACCCGCCATATCAATAGGG
CAGATGGTATTGGACTATCTAATAATGATCCGTTAGATAGCAGTGGAAAACCTATTTTTTATTGTACTGGTACAGAGGCA
ATTGAAGTCATTGACGAAATTGCTCCCAAGGAAGAAGATGTGGTGATTGACAAATTTCGCTGGAGTGGCTTTTATGAAAC
AAGTTTGGACATTATCCTAAAGAGTTTAAAGGTAAAGCATTTGATGATTGGCGGATTAGTTACAGATGGCTGTTTAATGA
CATCTGTTTTTGATGGGTATTTTAGAGATTATGAAATTAATCTCATAAAAGATATATGTGCAACTTCAAATGAAGGAGCA
CATATGGCTGCCACCCTGATTATGGCAAACTGGGTATATGGAATTAAAATATATGATTCAAGTGAATTAATTAAGAAATT
ACAGGGCAAAGATTATTATGTCTGGGAGTCCGAGGGGCCAGACCAATTACAATTTACCCCTGAGAATATGAGAGAAGTTT
ATGGAAAATTAAATCAAGATGCTAACTTGATTAAACAAAAATCTTAA

Upstream 100 bases:

>100_bases
GGACTAAAAGGATAATCGATGCACTAGAAAGCGGGGAAATATTTGAGTATTTTCCCATGGATAAAAAGTAAATTTAATTG
GAAAGGAGGTGCAAGGCGAT

Downstream 100 bases:

>100_bases
TAATTATTTAACAAGGAGGTTATAATTTAATGCGAAAGCTGAAATTAGTACTAGTGACAATGATAACAATTGTTTTATTA
ACTGCCTGTACCAATGGAGG

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MDLNKSALCLIDIQKESKFGIENIDSVVENSKHLIEQCRKLNIPVIYTRHINRADGIGLSNNDPLDSSGKPIFYCTGTEA
IEVIDEIAPKEEDVVIDKFRWSGFYETSLDIILKSLKVKHLMIGGLVTDGCLMTSVFDGYFRDYEINLIKDICATSNEGA
HMAATLIMANWVYGIKIYDSSELIKKLQGKDYYVWESEGPDQLQFTPENMREVYGKLNQDANLIKQKS

Sequences:

>Translated_228_residues
MDLNKSALCLIDIQKESKFGIENIDSVVENSKHLIEQCRKLNIPVIYTRHINRADGIGLSNNDPLDSSGKPIFYCTGTEA
IEVIDEIAPKEEDVVIDKFRWSGFYETSLDIILKSLKVKHLMIGGLVTDGCLMTSVFDGYFRDYEINLIKDICATSNEGA
HMAATLIMANWVYGIKIYDSSELIKKLQGKDYYVWESEGPDQLQFTPENMREVYGKLNQDANLIKQKS
>Mature_228_residues
MDLNKSALCLIDIQKESKFGIENIDSVVENSKHLIEQCRKLNIPVIYTRHINRADGIGLSNNDPLDSSGKPIFYCTGTEA
IEVIDEIAPKEEDVVIDKFRWSGFYETSLDIILKSLKVKHLMIGGLVTDGCLMTSVFDGYFRDYEINLIKDICATSNEGA
HMAATLIMANWVYGIKIYDSSELIKKLQGKDYYVWESEGPDQLQFTPENMREVYGKLNQDANLIKQKS

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=207, Percent_Identity=24.6376811594203, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 25844; Mature: 25844

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLNKSALCLIDIQKESKFGIENIDSVVENSKHLIEQCRKLNIPVIYTRHINRADGIGLS
CCCCCCEEEEEEECCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
NNDPLDSSGKPIFYCTGTEAIEVIDEIAPKEEDVVIDKFRWSGFYETSLDIILKSLKVKH
CCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
LMIGGLVTDGCLMTSVFDGYFRDYEINLIKDICATSNEGAHMAATLIMANWVYGIKIYDS
HEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEEECH
SELIKKLQGKDYYVWESEGPDQLQFTPENMREVYGKLNQDANLIKQKS
HHHHHHHCCCCEEEECCCCCCCEEECHHHHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure
MDLNKSALCLIDIQKESKFGIENIDSVVENSKHLIEQCRKLNIPVIYTRHINRADGIGLS
CCCCCCEEEEEEECCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
NNDPLDSSGKPIFYCTGTEAIEVIDEIAPKEEDVVIDKFRWSGFYETSLDIILKSLKVKH
CCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
LMIGGLVTDGCLMTSVFDGYFRDYEINLIKDICATSNEGAHMAATLIMANWVYGIKIYDS
HEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEEECH
SELIKKLQGKDYYVWESEGPDQLQFTPENMREVYGKLNQDANLIKQKS
HHHHHHHCCCCEEEECCCCCCCEEECHHHHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA