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The map label for this gene is spoIVB [H]

Identifier: 188586307

GI number: 188586307

Start: 1764841

End: 1766142

Strand: Reverse

Name: spoIVB [H]

Synonym: Nther_1690

Alternate gene names: 188586307

Gene position: 1766142-1764841 (Counterclockwise)

Preceding gene: 188586308

Following gene: 188586306

Centisome position: 55.79

GC content: 33.18

Gene sequence:

>1302_bases
TTGATAAACAAAGAAACTAGTAGAAAAATTACAGGGATTATCTTAGCAGCTGTAATTCTATTAGTTGGTTTTAGCAGTCC
CGTAAGAAATTATGTGGAATTACCCAATGAATTAAGGCTAATTAAGGGTGATAAACTGGAAGTTCATCCATTTTTACCAG
TAGAACTACACATATTAAATCAAGATCAAATTTTATCAGTCAATGGTCAACAAATTACTGATCAAACTCCCGCAGCAGTA
GGTTTAACAGAACCTTTAAAACTTTCTTCCTTAGATGTTGGTAATGTTAAAGTAGAGTTTCGTCTTTTTGGTTATGTTCC
CCTTAGAACAATGGAAATAAACGTAATTGAAGACAGGGAATTAGTACCAGGAGGCGAAGCCATTGGAATAAAAGCAGGAA
CTAGTGAAATAGTAGTTTCTGACTACTTTTATTTAGAAACTGAAAATGGTGAAATGTCACCAGCTAAAAAAGCTGGCATT
CAAAAAGGAGATAGCCTACTTGAGATAGAAGGTCATGATATTTCAAGTTTAGATTCTACTTCAGAATTATTAATTGATCT
ATTAGAAAAACAAGATACCGATTCAATTGAAGTGATTATTGAACGATCAGGAAAAGAAAAATCGAAAACAATTGATCCTC
GATACTGTTTAAACTTAAATGAATATGGTATTGGTTTGTATCTTACCGATTCTTCTCTAGGAGTTGGAACTTTAACTTAT
AAAGATTTTGAAAACAAAGAATATGGAGCTTTAGGCCATTCAATTTCAACATGGGATAGTGCTCCTGCTTCTTTAAAAGA
TGGTAGGATTGTAGATGCCAGGATAATAGACATTAACAAGGGTAGTGATGGAAAACCAGGAGAAAAAAAGGGAAATTTCC
TCCATGGTGAAGAACCTTTAGGTACTATTGAAAAAAATACTAAACATGGTATTTTTGGTAAAATCACTAATGAAAGTGTA
TACAGTTCCCAAATTGAACCCAAATCTATTGGATTGGCTAAACACGCAGAACCAGGATCAGCTCAAATTTTAACGGTTTT
AGATAATCAAGAGATAGAAAGATTTGAGATTGAAATCAAAAGAGTTTTTCCACACAATGCAGAAAATGGAAAAGGGATGG
TAATTGAAGTTACAGATGAAGAATTACTACAAAGAACTGGTGGTATTGTTCAAGGTATGAGTGGTAGTCCTATCATCCAA
GATGATAAATTAATTGGTGCAATTACACATGTATTTGTAAACGATTCAGAAAAGGGATATGGAACGTTTTTAGAGTGGAT
GCTCTATGAATCCGAAAAATAA

Upstream 100 bases:

>100_bases
TACAGTATTAGACATACTTATCGAGGGAAATTTAAAAATAGCGCTGCATCAATCAGCCGGGGAGGTGAAAAAATCTCAAA
TTAACTAGGAGTGTTGACAA

Downstream 100 bases:

>100_bases
TACACATTTGCACAAATTTCTGTTAATTGGCATCGAAGCGTGTGGATATTCGATGCCAATTTTTTTATAGGAAAATGAAG
GAACTATTTACTGATTTGTC

Product: stage IV sporulation protein B

Products: NA

Alternate protein names: Sporulation factor IV B protease; Stage IV sporulation protein B [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MINKETSRKITGIILAAVILLVGFSSPVRNYVELPNELRLIKGDKLEVHPFLPVELHILNQDQILSVNGQQITDQTPAAV
GLTEPLKLSSLDVGNVKVEFRLFGYVPLRTMEINVIEDRELVPGGEAIGIKAGTSEIVVSDYFYLETENGEMSPAKKAGI
QKGDSLLEIEGHDISSLDSTSELLIDLLEKQDTDSIEVIIERSGKEKSKTIDPRYCLNLNEYGIGLYLTDSSLGVGTLTY
KDFENKEYGALGHSISTWDSAPASLKDGRIVDARIIDINKGSDGKPGEKKGNFLHGEEPLGTIEKNTKHGIFGKITNESV
YSSQIEPKSIGLAKHAEPGSAQILTVLDNQEIERFEIEIKRVFPHNAENGKGMVIEVTDEELLQRTGGIVQGMSGSPIIQ
DDKLIGAITHVFVNDSEKGYGTFLEWMLYESEK

Sequences:

>Translated_433_residues
MINKETSRKITGIILAAVILLVGFSSPVRNYVELPNELRLIKGDKLEVHPFLPVELHILNQDQILSVNGQQITDQTPAAV
GLTEPLKLSSLDVGNVKVEFRLFGYVPLRTMEINVIEDRELVPGGEAIGIKAGTSEIVVSDYFYLETENGEMSPAKKAGI
QKGDSLLEIEGHDISSLDSTSELLIDLLEKQDTDSIEVIIERSGKEKSKTIDPRYCLNLNEYGIGLYLTDSSLGVGTLTY
KDFENKEYGALGHSISTWDSAPASLKDGRIVDARIIDINKGSDGKPGEKKGNFLHGEEPLGTIEKNTKHGIFGKITNESV
YSSQIEPKSIGLAKHAEPGSAQILTVLDNQEIERFEIEIKRVFPHNAENGKGMVIEVTDEELLQRTGGIVQGMSGSPIIQ
DDKLIGAITHVFVNDSEKGYGTFLEWMLYESEK
>Mature_433_residues
MINKETSRKITGIILAAVILLVGFSSPVRNYVELPNELRLIKGDKLEVHPFLPVELHILNQDQILSVNGQQITDQTPAAV
GLTEPLKLSSLDVGNVKVEFRLFGYVPLRTMEINVIEDRELVPGGEAIGIKAGTSEIVVSDYFYLETENGEMSPAKKAGI
QKGDSLLEIEGHDISSLDSTSELLIDLLEKQDTDSIEVIIERSGKEKSKTIDPRYCLNLNEYGIGLYLTDSSLGVGTLTY
KDFENKEYGALGHSISTWDSAPASLKDGRIVDARIIDINKGSDGKPGEKKGNFLHGEEPLGTIEKNTKHGIFGKITNESV
YSSQIEPKSIGLAKHAEPGSAQILTVLDNQEIERFEIEIKRVFPHNAENGKGMVIEVTDEELLQRTGGIVQGMSGSPIIQ
DDKLIGAITHVFVNDSEKGYGTFLEWMLYESEK

Specific function: Plays a central role in the sigma-K checkpoint which coordinates gene expression during the later stages of spore formation. The protease is activated by trans cleavage of the zymogen precursor producing SpoIVB-45 kDa. This undergoes further trimming by c

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Forespore intermembrane space [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase S55 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR014219
- InterPro:   IPR008763 [H]

Pfam domain/function: PF00595 PDZ; PF05580 Peptidase_S55 [H]

EC number: =3.4.21.116 [H]

Molecular weight: Translated: 47612; Mature: 47612

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKETSRKITGIILAAVILLVGFSSPVRNYVELPNELRLIKGDKLEVHPFLPVELHILN
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEECCEEEECCCCCEEEEEEC
QDQILSVNGQQITDQTPAAVGLTEPLKLSSLDVGNVKVEFRLFGYVPLRTMEINVIEDRE
CCEEEEECCCEECCCCCCEECCCCCCEECCCCCCCEEEEEEEEEEEEEEEEEEEEEECCE
LVPGGEAIGIKAGTSEIVVSDYFYLETENGEMSPAKKAGIQKGDSLLEIEGHDISSLDST
ECCCCCEEEEECCCCEEEEEEEEEEECCCCCCCCHHHHCCCCCCCEEEECCCCCCCCCCH
SELLIDLLEKQDTDSIEVIIERSGKEKSKTIDPRYCLNLNEYGIGLYLTDSSLGVGTLTY
HHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCEEEEEE
KDFENKEYGALGHSISTWDSAPASLKDGRIVDARIIDINKGSDGKPGEKKGNFLHGEEPL
ECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCC
GTIEKNTKHGIFGKITNESVYSSQIEPKSIGLAKHAEPGSAQILTVLDNQEIERFEIEIK
CCCCCCCCCCEEEEECCCCHHHCCCCHHHCCCCCCCCCCCCEEEEEECCCCCEEEEEEEE
RVFPHNAENGKGMVIEVTDEELLQRTGGIVQGMSGSPIIQDDKLIGAITHVFVNDSEKGY
EECCCCCCCCCEEEEEECHHHHHHHCCCEEECCCCCCCEECCCCEEEEEEEEECCCCCCH
GTFLEWMLYESEK
HHHEEEEEEECCC
>Mature Secondary Structure
MINKETSRKITGIILAAVILLVGFSSPVRNYVELPNELRLIKGDKLEVHPFLPVELHILN
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEECCEEEECCCCCEEEEEEC
QDQILSVNGQQITDQTPAAVGLTEPLKLSSLDVGNVKVEFRLFGYVPLRTMEINVIEDRE
CCEEEEECCCEECCCCCCEECCCCCCEECCCCCCCEEEEEEEEEEEEEEEEEEEEEECCE
LVPGGEAIGIKAGTSEIVVSDYFYLETENGEMSPAKKAGIQKGDSLLEIEGHDISSLDST
ECCCCCEEEEECCCCEEEEEEEEEEECCCCCCCCHHHHCCCCCCCEEEECCCCCCCCCCH
SELLIDLLEKQDTDSIEVIIERSGKEKSKTIDPRYCLNLNEYGIGLYLTDSSLGVGTLTY
HHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCEEEEEE
KDFENKEYGALGHSISTWDSAPASLKDGRIVDARIIDINKGSDGKPGEKKGNFLHGEEPL
ECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCC
GTIEKNTKHGIFGKITNESVYSSQIEPKSIGLAKHAEPGSAQILTVLDNQEIERFEIEIK
CCCCCCCCCCEEEEECCCCHHHCCCCHHHCCCCCCCCCCCCEEEEEECCCCCEEEEEEEE
RVFPHNAENGKGMVIEVTDEELLQRTGGIVQGMSGSPIIQDDKLIGAITHVFVNDSEKGY
EECCCCCCCCCEEEEEECHHHHHHHCCCEEECCCCCCCEECCCCEEEEEEEEECCCCCCH
GTFLEWMLYESEK
HHHEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 11741860 [H]