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The map label for this gene is cca [H]

Identifier: 188586271

GI number: 188586271

Start: 1731112

End: 1733784

Strand: Reverse

Name: cca [H]

Synonym: Nther_1654

Alternate gene names: 188586271

Gene position: 1733784-1731112 (Counterclockwise)

Preceding gene: 188586272

Following gene: 188586270

Centisome position: 54.77

GC content: 32.59

Gene sequence:

>2673_bases
ATGAAAGTAGTGACATCACATCAAAATTTAGATTTCGATGGATTAGCCGCAATGTTAGCTTATAATAAATTGAATCCTGA
CACTAAAATGGTACTACCACCAAAATTAAATCAAAATGTCAGAGCTTTTTACTCACTATACAAAGATACTTTTTCATTTG
TTGAAAGAATCAATTTTGATTTACATAAAGTAAATACCTTGACCATGGTAGATACTAGTGATGAAAAACGCATTGGAAAA
TTATCATCTATTTTACCGCAAGTGGAACAGCTAATCATCTACGACCATCATGAATACAAAAATCTTTCTCAAAACCCTAC
AATAGAGATTAATCATGAAGTAGGGGCAACAAGTACAATTCTTGTTGAAGAATTAATGAACAAAGAAATTGAATTATCTC
CCTTGGAAGCAACTGTAATAGCCATGGGGATTTATGAAGATACAGGGAACCTGACATTTTCTCATGTTACTGCTAGGGAT
GTGAGAGCCGTTGCCCAACTCCTAGAGTGGGGAGCAGATGTGGATATTATCAGGGACTATGTAACCATCATTTTAACACA
AGCTCAAAGAGGTCTATTAGATGAACTTATATCTAATACCAAGTATTTAAATATTAATGATTATGAAATTGGACTTTCTA
TAACAGAACGGGAAGAATATTTCAGTGGAGCGGCAGCTTTAGTACATAAGTTAATGGAAATTGAAGATGTAGACTTATTT
TTTATTATAGTAAAAATGGACAAGAAAGTATTCGTTATTGGTAGAAGTCGAAGAGAAGAAATTAATGTCAGCAGGATCTT
GTCTCCTTTTAGTGGAAAAGGACATGATCAAGCTGCCTCTGCTACTATAAAACATGGTGATCTACAACATATCGAAACTG
AACTTATTGAATCCTTAAAATTGCATTTACCTGTTCTATTAACTGCTAAAGCTATCATGTCAGCTCCAGTTAAAACAATT
TCGTCATCTACTACAATACAAGAAGCTGATCATTTATTACATAAATACGGTCATAGTGGTTTACCAGTAGTACAAGATAA
ACAGAATGATATTGATGATGAAGCTGGTAAAATTGTTGGAGTTATTTCTAGAAGAGATATAGAAAAAGCTAAACATCATG
GATTTGGTCATTCTCCGGTCAAGGGGTATATGTCTCAAAAAGTTATTTCTATTTCACCGGACACTTCTATTAAGGAAATT
CAACACTTAATGGTATCTAATGATATCGGACGTTTGCCAGTTATTGATTCAAATGCCAATTTGATAGGAATAGTTACTAG
AACGAATTTATTAAAAATACAGCACGGTCAATTAACTGAGGAACAAGAGCGAACTAATTTATACTTAAAAGAACTTGATG
AATTTTCTGAAGATATTACTGAATTAATGGCACGTCGGTTACCTAGCAAAATTCTTGGAGTTTTATATTTAATTGGCCAA
AAGGCAGATAAAGAAGGTTTTAAAGTTTATGGTGTTGGCGGATTTATAAGAGATTTACTTTTAGGTAAAGATAATTTGGA
TATAGATTTAGTCATAGAACAAGATGCCATTAGTTTTGCAAAATTAGTCAGTAAACACTTGAATGGAGATTTAAAAACAT
TTTCTCAATTTCAAACTGCTCGTTTAACCTTGCGTTCAGGCACGAGAATAGATTTTGCCACAGCTAGAATAGAATATTAT
GCTTTTCCTGCAGCATCACCGGAAGTTGAAGAAAGTACCATAAAACAAGATCTATACAGGCGAGATTTTACCATAAATAC
ATTAGCTGTAGAGTTAAATTGTAATTCCTTTGGGAAATTATTGGATTTCTTTGGTGGAACAAAAGATCTAAAAAAAGGAG
TTATCAGGGTATTATATAATCTAAGTTTTGTAGAAGATCCTACAAGAATTTTTCGTGCTATTAGGTTTGAATCACGATTC
AATTTCAATATTGAAGAGCAAACTCTAATTTTCATAAAAAATAGCTTGGAAACAGGTGTACTTGATAAACTACCTGGTGA
GAGATTGTATGAAGAACTGCGAAATATAGTTGACGAAGATGAAGCCGTAAACACTTTTCGCCGTATGGATGAACTGGGGA
TATTTACAAAAATATTTCCAAATTTAAATGTATCTCAAGATAAGTTAGTAAAATTGCAAAACATTTATGATATTTTGAAC
TGGTATGAACGAGAAAATAAACAAAAACAAGAAAAGTTTGTCAGTAAAGAAGCTATGGTATTTTCATGCTTGTTGCAGGA
TCAACCGTTACCGATAATTAGCTCGATTTTAGAACGTTTAAAAGTGCCACAAAAAATAAGGGATGTAATTATAACAACAG
TTAAAGAAACGGAATCCCTTTCTAGTAAATTAGAGTATACCGAGAAAAATAGTGAACTAGTACAAAATTTAGAAGAGATT
CCTCTGGAAACTATTCTATTTGTGTTAGCTGATAAGGAGAATCAAAAAATAAAGAACAAAATATATTACTATCTAGAGGA
GTTAATTGGAGCTGGAGTTAGTATTACTGGGGAAGATCTTAAAACTCTAGGGATAAAGCCAGGTCCTGTTTATAAAACGG
CTTTAGAAGAAGTTAGAAAAGCAAGATTAGACGGACTGGTTACAACTCCTGAAGAAGAATTAGAATATGTGCTAGATTTT
TTTGAACAGAAAGGAGAAGATATGCATGGATAA

Upstream 100 bases:

>100_bases
CCCCTTTAGAGCTACTGTTTAAAGGGGTTGCTTTTCTTTTGTTCCCTGTATAACTGTGATATAATGTAACGAGTAAATAA
AAGGGGGGGCTGATCCGGAC

Downstream 100 bases:

>100_bases
TATATTTATAGGAGACATTTTGCATATAATTCCGGCACTTTTAATTGCTTTAACTGTTCATGAATACTCTCATGGTAGAA
TAGCCTATTATTTAGGTGAT

Product: CBS domain containing protein

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 890; Mature: 890

Protein sequence:

>890_residues
MKVVTSHQNLDFDGLAAMLAYNKLNPDTKMVLPPKLNQNVRAFYSLYKDTFSFVERINFDLHKVNTLTMVDTSDEKRIGK
LSSILPQVEQLIIYDHHEYKNLSQNPTIEINHEVGATSTILVEELMNKEIELSPLEATVIAMGIYEDTGNLTFSHVTARD
VRAVAQLLEWGADVDIIRDYVTIILTQAQRGLLDELISNTKYLNINDYEIGLSITEREEYFSGAAALVHKLMEIEDVDLF
FIIVKMDKKVFVIGRSRREEINVSRILSPFSGKGHDQAASATIKHGDLQHIETELIESLKLHLPVLLTAKAIMSAPVKTI
SSSTTIQEADHLLHKYGHSGLPVVQDKQNDIDDEAGKIVGVISRRDIEKAKHHGFGHSPVKGYMSQKVISISPDTSIKEI
QHLMVSNDIGRLPVIDSNANLIGIVTRTNLLKIQHGQLTEEQERTNLYLKELDEFSEDITELMARRLPSKILGVLYLIGQ
KADKEGFKVYGVGGFIRDLLLGKDNLDIDLVIEQDAISFAKLVSKHLNGDLKTFSQFQTARLTLRSGTRIDFATARIEYY
AFPAASPEVEESTIKQDLYRRDFTINTLAVELNCNSFGKLLDFFGGTKDLKKGVIRVLYNLSFVEDPTRIFRAIRFESRF
NFNIEEQTLIFIKNSLETGVLDKLPGERLYEELRNIVDEDEAVNTFRRMDELGIFTKIFPNLNVSQDKLVKLQNIYDILN
WYERENKQKQEKFVSKEAMVFSCLLQDQPLPIISSILERLKVPQKIRDVIITTVKETESLSSKLEYTEKNSELVQNLEEI
PLETILFVLADKENQKIKNKIYYYLEELIGAGVSITGEDLKTLGIKPGPVYKTALEEVRKARLDGLVTTPEEELEYVLDF
FEQKGEDMHG

Sequences:

>Translated_890_residues
MKVVTSHQNLDFDGLAAMLAYNKLNPDTKMVLPPKLNQNVRAFYSLYKDTFSFVERINFDLHKVNTLTMVDTSDEKRIGK
LSSILPQVEQLIIYDHHEYKNLSQNPTIEINHEVGATSTILVEELMNKEIELSPLEATVIAMGIYEDTGNLTFSHVTARD
VRAVAQLLEWGADVDIIRDYVTIILTQAQRGLLDELISNTKYLNINDYEIGLSITEREEYFSGAAALVHKLMEIEDVDLF
FIIVKMDKKVFVIGRSRREEINVSRILSPFSGKGHDQAASATIKHGDLQHIETELIESLKLHLPVLLTAKAIMSAPVKTI
SSSTTIQEADHLLHKYGHSGLPVVQDKQNDIDDEAGKIVGVISRRDIEKAKHHGFGHSPVKGYMSQKVISISPDTSIKEI
QHLMVSNDIGRLPVIDSNANLIGIVTRTNLLKIQHGQLTEEQERTNLYLKELDEFSEDITELMARRLPSKILGVLYLIGQ
KADKEGFKVYGVGGFIRDLLLGKDNLDIDLVIEQDAISFAKLVSKHLNGDLKTFSQFQTARLTLRSGTRIDFATARIEYY
AFPAASPEVEESTIKQDLYRRDFTINTLAVELNCNSFGKLLDFFGGTKDLKKGVIRVLYNLSFVEDPTRIFRAIRFESRF
NFNIEEQTLIFIKNSLETGVLDKLPGERLYEELRNIVDEDEAVNTFRRMDELGIFTKIFPNLNVSQDKLVKLQNIYDILN
WYERENKQKQEKFVSKEAMVFSCLLQDQPLPIISSILERLKVPQKIRDVIITTVKETESLSSKLEYTEKNSELVQNLEEI
PLETILFVLADKENQKIKNKIYYYLEELIGAGVSITGEDLKTLGIKPGPVYKTALEEVRKARLDGLVTTPEEELEYVLDF
FEQKGEDMHG
>Mature_890_residues
MKVVTSHQNLDFDGLAAMLAYNKLNPDTKMVLPPKLNQNVRAFYSLYKDTFSFVERINFDLHKVNTLTMVDTSDEKRIGK
LSSILPQVEQLIIYDHHEYKNLSQNPTIEINHEVGATSTILVEELMNKEIELSPLEATVIAMGIYEDTGNLTFSHVTARD
VRAVAQLLEWGADVDIIRDYVTIILTQAQRGLLDELISNTKYLNINDYEIGLSITEREEYFSGAAALVHKLMEIEDVDLF
FIIVKMDKKVFVIGRSRREEINVSRILSPFSGKGHDQAASATIKHGDLQHIETELIESLKLHLPVLLTAKAIMSAPVKTI
SSSTTIQEADHLLHKYGHSGLPVVQDKQNDIDDEAGKIVGVISRRDIEKAKHHGFGHSPVKGYMSQKVISISPDTSIKEI
QHLMVSNDIGRLPVIDSNANLIGIVTRTNLLKIQHGQLTEEQERTNLYLKELDEFSEDITELMARRLPSKILGVLYLIGQ
KADKEGFKVYGVGGFIRDLLLGKDNLDIDLVIEQDAISFAKLVSKHLNGDLKTFSQFQTARLTLRSGTRIDFATARIEYY
AFPAASPEVEESTIKQDLYRRDFTINTLAVELNCNSFGKLLDFFGGTKDLKKGVIRVLYNLSFVEDPTRIFRAIRFESRF
NFNIEEQTLIFIKNSLETGVLDKLPGERLYEELRNIVDEDEAVNTFRRMDELGIFTKIFPNLNVSQDKLVKLQNIYDILN
WYERENKQKQEKFVSKEAMVFSCLLQDQPLPIISSILERLKVPQKIRDVIITTVKETESLSSKLEYTEKNSELVQNLEEI
PLETILFVLADKENQKIKNKIYYYLEELIGAGVSITGEDLKTLGIKPGPVYKTALEEVRKARLDGLVTTPEEELEYVLDF
FEQKGEDMHG

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=232, Percent_Identity=29.3103448275862, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI87081691, Length=254, Percent_Identity=29.5275590551181, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321016, Length=179, Percent_Identity=31.8435754189944, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 101203; Mature: 101203

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVTSHQNLDFDGLAAMLAYNKLNPDTKMVLPPKLNQNVRAFYSLYKDTFSFVERINFD
CCEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
LHKVNTLTMVDTSDEKRIGKLSSILPQVEQLIIYDHHEYKNLSQNPTIEINHEVGATSTI
CEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHEECCHHHCCCCCCCCEEEECCCCCHHHH
LVEELMNKEIELSPLEATVIAMGIYEDTGNLTFSHVTARDVRAVAQLLEWGADVDIIRDY
HHHHHHCCCCCCCCCCEEEEEEEEEECCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHH
VTIILTQAQRGLLDELISNTKYLNINDYEIGLSITEREEYFSGAAALVHKLMEIEDVDLF
HHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCEE
FIIVKMDKKVFVIGRSRREEINVSRILSPFSGKGHDQAASATIKHGDLQHIETELIESLK
EEEEEECCEEEEEECCCHHHCCHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHH
LHLPVLLTAKAIMSAPVKTISSSTTIQEADHLLHKYGHSGLPVVQDKQNDIDDEAGKIVG
HHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCEEE
VISRRDIEKAKHHGFGHSPVKGYMSQKVISISPDTSIKEIQHLMVSNDIGRLPVIDSNAN
EHHHHHHHHHHHCCCCCCCHHHHHCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCC
LIGIVTRTNLLKIQHGQLTEEQERTNLYLKELDEFSEDITELMARRLPSKILGVLYLIGQ
EEEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
KADKEGFKVYGVGGFIRDLLLGKDNLDIDLVIEQDAISFAKLVSKHLNGDLKTFSQFQTA
CCCCCCCEEEECHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHH
RLTLRSGTRIDFATARIEYYAFPAASPEVEESTIKQDLYRRDFTINTLAVELNCNSFGKL
HEEECCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCHHHHH
LDFFGGTKDLKKGVIRVLYNLSFVEDPTRIFRAIRFESRFNFNIEEQTLIFIKNSLETGV
HHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCC
LDKLPGERLYEELRNIVDEDEAVNTFRRMDELGIFTKIFPNLNVSQDKLVKLQNIYDILN
HHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
WYERENKQKQEKFVSKEAMVFSCLLQDQPLPIISSILERLKVPQKIRDVIITTVKETESL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SSKLEYTEKNSELVQNLEEIPLETILFVLADKENQKIKNKIYYYLEELIGAGVSITGEDL
HHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCH
KTLGIKPGPVYKTALEEVRKARLDGLVTTPEEELEYVLDFFEQKGEDMHG
HHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKVVTSHQNLDFDGLAAMLAYNKLNPDTKMVLPPKLNQNVRAFYSLYKDTFSFVERINFD
CCEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
LHKVNTLTMVDTSDEKRIGKLSSILPQVEQLIIYDHHEYKNLSQNPTIEINHEVGATSTI
CEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHEECCHHHCCCCCCCCEEEECCCCCHHHH
LVEELMNKEIELSPLEATVIAMGIYEDTGNLTFSHVTARDVRAVAQLLEWGADVDIIRDY
HHHHHHCCCCCCCCCCEEEEEEEEEECCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHH
VTIILTQAQRGLLDELISNTKYLNINDYEIGLSITEREEYFSGAAALVHKLMEIEDVDLF
HHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCEE
FIIVKMDKKVFVIGRSRREEINVSRILSPFSGKGHDQAASATIKHGDLQHIETELIESLK
EEEEEECCEEEEEECCCHHHCCHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHH
LHLPVLLTAKAIMSAPVKTISSSTTIQEADHLLHKYGHSGLPVVQDKQNDIDDEAGKIVG
HHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCEEE
VISRRDIEKAKHHGFGHSPVKGYMSQKVISISPDTSIKEIQHLMVSNDIGRLPVIDSNAN
EHHHHHHHHHHHCCCCCCCHHHHHCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCC
LIGIVTRTNLLKIQHGQLTEEQERTNLYLKELDEFSEDITELMARRLPSKILGVLYLIGQ
EEEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
KADKEGFKVYGVGGFIRDLLLGKDNLDIDLVIEQDAISFAKLVSKHLNGDLKTFSQFQTA
CCCCCCCEEEECHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHH
RLTLRSGTRIDFATARIEYYAFPAASPEVEESTIKQDLYRRDFTINTLAVELNCNSFGKL
HEEECCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCHHHHH
LDFFGGTKDLKKGVIRVLYNLSFVEDPTRIFRAIRFESRFNFNIEEQTLIFIKNSLETGV
HHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCC
LDKLPGERLYEELRNIVDEDEAVNTFRRMDELGIFTKIFPNLNVSQDKLVKLQNIYDILN
HHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
WYERENKQKQEKFVSKEAMVFSCLLQDQPLPIISSILERLKVPQKIRDVIITTVKETESL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SSKLEYTEKNSELVQNLEEIPLETILFVLADKENQKIKNKIYYYLEELIGAGVSITGEDL
HHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCH
KTLGIKPGPVYKTALEEVRKARLDGLVTTPEEELEYVLDFFEQKGEDMHG
HHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA