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The map label for this gene is mdeA [H]

Identifier: 188585786

GI number: 188585786

Start: 1228004

End: 1229203

Strand: Direct

Name: mdeA [H]

Synonym: Nther_1160

Alternate gene names: 188585786

Gene position: 1228004-1229203 (Clockwise)

Preceding gene: 188585785

Following gene: 188585787

Centisome position: 38.79

GC content: 39.33

Gene sequence:

>1200_bases
ATGGAAAAACGTAGGTTATCATTTAATGCTAAGGCTATTCATGCAGGTAGTGAACCCTGCCCTACTACAGGAGCTCATGT
AGCGCCAGTTTACCGGACTTCAACCTTTGTTATTGATAATGTCGAGAGAGCTATCAGAATAGGCTATGGAGAAGAGGAAG
GATATTCTTATGGTAGATTTGGAAACCCCACGGTAGACGCTTTACAAGAAAAAATTGCTTCTCTTGAAAATGCTGAAGCA
GCTTTGGCTACGGCTTCAGGCATGGCAGCAATTTCTACTGCATTAATGGCAGAACTACAAGCTGGTGACCATATTGTGAC
AGGTGATATCATCTATGGTTGTACCTATGGTTTTGTCCGCGATCTTTTACCTCAATACGGAATTGAAGTGACTATGGTTG
ATACAACAGATCCTAAAAACATTGAAGAAGCAATCAAACCAAATACTAAAATAGTATATATTGAAACTCCTTCAAATCCT
GTATTGGGGATCACTGATATTGAAAAATCAGCTGAACTTGCACATGCTAATGGTGCTAAACTGTTTGTTGACAATACTTG
GGCAACTCCTTATCTACAAAATCCACTAAATATAGGAGCAGATGTTGTACTTCATAGTGCAACAAAATACTTGAATGGTC
ATGGAGACATTGTTGGTGGAGCTATAGTAGGTTCAAAAGAGTTAATGGATAAAATCAAAGCACCATATCTTGAATTCTTC
GGTGGAATTATGTCTCCAATGGATGCAGCAGAAGTATCTAAAGGAATTAAAACACTAGGAGTGCGAATGGAAAAACACTG
CCAAAACGCTAAAAAGATAGCTGAATTCCTAGAAGGCCATCATATGGTAGAAAGAGTTCTATACCCTGGTGTTGAAACTC
ATCCGCAACACGAGCTAGCCAAAAAGCAGATGAAAGATTTTGGTGGCATGATGAGTTTTGATGTCAAAGGTGGTGCAAAG
GCAGGAAAAGCCCTTATGAACAGTGTTGAACTAGTTAGTCTTGCTACTAGTCTTGGCTGTGTAGATTCTCTGATTCAGCA
TTCACCTTCTATGAGTCATGCCACAATGAGTAAAGAAGAACGAGAGCGTGTAGGTATTACTGAAGGACAAGTGCGATTAT
CTGTAGGAATTGAAGAGCCTGAAAATGTCATTGCTGATCTAAAACAAGCCCTAGAGCAAGTTGAAAAAGTTATAGATTAA

Upstream 100 bases:

>100_bases
GAGTTACCAAAGATGGTTGTGAAGCTTTGGCAGACTTCCCAAGGAAGCTGGCAAGTAAATCAGCCTAAATATAAATTAAA
ATTAAGACGGAGGGATATTT

Downstream 100 bases:

>100_bases
TCTAACTAGCTACTGAAACAGGGTATTTAAAAGCCTATAGATATATAGTATTATATATCTATAGGCTTTATTAACATAAA
AAAAACTGATAAACTGTTAT

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MEKRRLSFNAKAIHAGSEPCPTTGAHVAPVYRTSTFVIDNVERAIRIGYGEEEGYSYGRFGNPTVDALQEKIASLENAEA
ALATASGMAAISTALMAELQAGDHIVTGDIIYGCTYGFVRDLLPQYGIEVTMVDTTDPKNIEEAIKPNTKIVYIETPSNP
VLGITDIEKSAELAHANGAKLFVDNTWATPYLQNPLNIGADVVLHSATKYLNGHGDIVGGAIVGSKELMDKIKAPYLEFF
GGIMSPMDAAEVSKGIKTLGVRMEKHCQNAKKIAEFLEGHHMVERVLYPGVETHPQHELAKKQMKDFGGMMSFDVKGGAK
AGKALMNSVELVSLATSLGCVDSLIQHSPSMSHATMSKEERERVGITEGQVRLSVGIEEPENVIADLKQALEQVEKVID

Sequences:

>Translated_399_residues
MEKRRLSFNAKAIHAGSEPCPTTGAHVAPVYRTSTFVIDNVERAIRIGYGEEEGYSYGRFGNPTVDALQEKIASLENAEA
ALATASGMAAISTALMAELQAGDHIVTGDIIYGCTYGFVRDLLPQYGIEVTMVDTTDPKNIEEAIKPNTKIVYIETPSNP
VLGITDIEKSAELAHANGAKLFVDNTWATPYLQNPLNIGADVVLHSATKYLNGHGDIVGGAIVGSKELMDKIKAPYLEFF
GGIMSPMDAAEVSKGIKTLGVRMEKHCQNAKKIAEFLEGHHMVERVLYPGVETHPQHELAKKQMKDFGGMMSFDVKGGAK
AGKALMNSVELVSLATSLGCVDSLIQHSPSMSHATMSKEERERVGITEGQVRLSVGIEEPENVIADLKQALEQVEKVID
>Mature_399_residues
MEKRRLSFNAKAIHAGSEPCPTTGAHVAPVYRTSTFVIDNVERAIRIGYGEEEGYSYGRFGNPTVDALQEKIASLENAEA
ALATASGMAAISTALMAELQAGDHIVTGDIIYGCTYGFVRDLLPQYGIEVTMVDTTDPKNIEEAIKPNTKIVYIETPSNP
VLGITDIEKSAELAHANGAKLFVDNTWATPYLQNPLNIGADVVLHSATKYLNGHGDIVGGAIVGSKELMDKIKAPYLEFF
GGIMSPMDAAEVSKGIKTLGVRMEKHCQNAKKIAEFLEGHHMVERVLYPGVETHPQHELAKKQMKDFGGMMSFDVKGGAK
AGKALMNSVELVSLATSLGCVDSLIQHSPSMSHATMSKEERERVGITEGQVRLSVGIEEPENVIADLKQALEQVEKVID

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=389, Percent_Identity=40.3598971722365, Blast_Score=283, Evalue=1e-76,
Organism=Homo sapiens, GI299473759, Length=389, Percent_Identity=36.5038560411311, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI25453487, Length=388, Percent_Identity=35.0515463917526, Blast_Score=220, Evalue=2e-57,
Organism=Escherichia coli, GI1790375, Length=385, Percent_Identity=35.8441558441558, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI1789383, Length=394, Percent_Identity=28.6802030456853, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17538055, Length=393, Percent_Identity=41.7302798982188, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17560056, Length=393, Percent_Identity=42.4936386768448, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI71980708, Length=394, Percent_Identity=37.8172588832487, Blast_Score=273, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6319307, Length=396, Percent_Identity=41.6666666666667, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=32.7981651376147, Blast_Score=208, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6321254, Length=393, Percent_Identity=32.5699745547074, Blast_Score=169, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6321170, Length=326, Percent_Identity=29.7546012269939, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6321904, Length=384, Percent_Identity=27.8645833333333, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922554, Length=394, Percent_Identity=41.8781725888325, Blast_Score=305, Evalue=5e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 43116; Mature: 43116

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKRRLSFNAKAIHAGSEPCPTTGAHVAPVYRTSTFVIDNVERAIRIGYGEEEGYSYGRF
CCCCCCCCCCEEEECCCCCCCCCCCCCCEEEECCEEEECCHHHHHEECCCCCCCCCCCCC
GNPTVDALQEKIASLENAEAALATASGMAAISTALMAELQAGDHIVTGDIIYGCTYGFVR
CCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHH
DLLPQYGIEVTMVDTTDPKNIEEAIKPNTKIVYIETPSNPVLGITDIEKSAELAHANGAK
HHHHHHCCEEEEEECCCCCHHHHHHCCCCEEEEEECCCCCEEEEECCHHHHHHHCCCCCE
LFVDNTWATPYLQNPLNIGADVVLHSATKYLNGHGDIVGGAIVGSKELMDKIKAPYLEFF
EEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCEEECCHHHHHHHCCHHHHHH
GGIMSPMDAAEVSKGIKTLGVRMEKHCQNAKKIAEFLEGHHMVERVLYPGVETHPQHELA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
KKQMKDFGGMMSFDVKGGAKAGKALMNSVELVSLATSLGCVDSLIQHSPSMSHATMSKEE
HHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCHHH
RERVGITEGQVRLSVGIEEPENVIADLKQALEQVEKVID
HHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKRRLSFNAKAIHAGSEPCPTTGAHVAPVYRTSTFVIDNVERAIRIGYGEEEGYSYGRF
CCCCCCCCCCEEEECCCCCCCCCCCCCCEEEECCEEEECCHHHHHEECCCCCCCCCCCCC
GNPTVDALQEKIASLENAEAALATASGMAAISTALMAELQAGDHIVTGDIIYGCTYGFVR
CCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHH
DLLPQYGIEVTMVDTTDPKNIEEAIKPNTKIVYIETPSNPVLGITDIEKSAELAHANGAK
HHHHHHCCEEEEEECCCCCHHHHHHCCCCEEEEEECCCCCEEEEECCHHHHHHHCCCCCE
LFVDNTWATPYLQNPLNIGADVVLHSATKYLNGHGDIVGGAIVGSKELMDKIKAPYLEFF
EEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCEEECCHHHHHHHCCHHHHHH
GGIMSPMDAAEVSKGIKTLGVRMEKHCQNAKKIAEFLEGHHMVERVLYPGVETHPQHELA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
KKQMKDFGGMMSFDVKGGAKAGKALMNSVELVSLATSLGCVDSLIQHSPSMSHATMSKEE
HHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCHHH
RERVGITEGQVRLSVGIEEPENVIADLKQALEQVEKVID
HHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]