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The map label for this gene is fusA [H]

Identifier: 188584652

GI number: 188584652

Start: 18000

End: 20078

Strand: Direct

Name: fusA [H]

Synonym: Nther_0010

Alternate gene names: 188584652

Gene position: 18000-20078 (Clockwise)

Preceding gene: 188584651

Following gene: 188584653

Centisome position: 0.57

GC content: 37.42

Gene sequence:

>2079_bases
ATGAAGAGTTATCAACCGGATCAAATTAGAAATGTGGGATTATGTTCTCATGGTGGTGCGGGCAAGACATCCTTAACCGA
AGCTCTACTGTACTCTTCCAACTCCATTGATCGACTAGGTAATGTAGAAGATGGTACTACTACTACAGATTTTGATAAAG
AAGAAGTAGATCGAGGTATTACCATCAATACTTCAGTGGCCCCTTGTGAGTGGAAAGGTCATAAGCTCAATTTAGTGGAT
ACCCCGGGATATTTTGACTTCATTGGCGATGTGAAAAGTTCTTTGCGTATCGTAGATGGAGCTTTAATAGTAGTTTGTGC
TGCTTCTGGTGTGCAAGTTGGGACAGAACAAGTATGGCATTATGCTGATGAAAATGACCTACCAAGAGTGGTATTTATAA
ATAAAATGGAAAGAGAAAATGCCAGCTTTAATAGGACTTTAGAACAATTAAGGGAAAATTTTGGGGATCAAGTTGTTCCC
GTTATGATACCAATCGGAAGTGAAGATTCCTTTGAAGGTGTAATTAATTTAGTCACGATGAAAGCTAATTACCATAAAGG
AGATGGCAAGGAGTTTAGCGAAGAAGAGATTCCTTCAGAATATCAAGAAGAAGCAGATAATTTTCGGGAAATGCTAATGG
AAGCTGTGGCAGAATGTGATGATGAAATGCTAGAAAAATATTTAGAGGGCGAATCTTTAACAGAAGATGAGGTCATCAAT
GGAGTGAAACAATTTACAAAAGAAGGAGAACTTATTCCCGTCCTTTGTGGCTCAGCAATTCAGAATATTGGTGTAACTCA
GTTGATGGATTGCCTTAAATACTATATTCCTAGTCCGGTTGATTTAAATTATCAAGGTTTTAATCCTGAGACTGAGGAGG
AAGTTAAGATAAATCCATCTGAAGAGGGACCCTTCAGTGCATTTGTCTTTAAAACCGTGGCAGACCCCTATGTGGGTAGA
CTGAATTATTTCAGAGTATATTCTGGCCAACTTTCTGACGACAAAGAAGCATATAATTCTAATAATAAAACCTCTGAAAG
AATTGGTAAGTTGTATTATTTAAAAGGAAAAGAACAAATCCCAGTGAATACTGTTTCTGTTGGAGATATAGCTGCAGTTA
GTAAATTACAGAAAACTGCTACAGGGGATACACTATCAGATTCTGAAAAACCAGTTAAGCTACCAGAAGTTGAATTCCCA
GATCCGGTCATTTCTTTTGCTGTTGTCACACAAGATAAAGGTGACGAAGAAAAAGTCGGTACAAGTTTGTCTAAATTTTT
AGAAGAAGATCCAATTTTAAATTTAGAGAAGAATGACGAAACTAAAGAAACACTAATTTCTGGAATGGGAGAATTGCAGC
TTGAAATAATAATTGAGAAAATGAAGAAAAAGTACGGAGTAGATGTAGAGTTGAAAACTCCCAAGGTTCCCTACAAAGAA
ACAATTACTAAAACAGTTAATGCTGAAGAAAAATATAAAAAACAATCAGGTGGAAGAGGGCAATATGGCCATGTGTTTAT
TGAATTTTCACCCTTACAAGAAGGGGAAGGTTTTCAGTTTGAAAACAATATTGTTGGCGGAGTAATTCCCAAAACATATA
TTCCGGCTGTAGAAAAAGGAATATTTGAGGCTATGGAGACAGGAGTTTTGGCAGGATATCCTGTGATTGATATTAAAGCT
AACCTATACGATGGTTCTCACCACAGTGTTGATTCTTCTGAAATGGCCTTCAAAGTTGCAGGTGCCAACGCATTCAAAAA
AGGAATGCAGAATGCTCAACCAGTACTTTTAGAGCCAGTAATGGAAGTAGAAGTAAGAGTTCCAGAAAAATTCATGGGAG
ATATTATGGGTGATATAAATAGTAAACGGGGTAAAATCCAGGGAATGGACCCTGATGGTGATTATCAAATAATAAAAGCT
TATGTACCCCAAGCAGAGATGTTAAGGTACGCAATTGACTTGAGATCTATGGCTCAGGGTAGAGGGACATTCACTTCTGT
ATTTAGCCATTACGAGCGAGTACCTGATGATGTTAGAGATGAGATCATTGAAGAGACTACTGCAGAGAAGGAAGCATAA

Upstream 100 bases:

>100_bases
ACCTCTAGTTGTCAGTATTTTTTACGAAAATTAGTGAAAAAATTATTAAAATTAAATTTTAGAAAAGTTAAATAAAAAGC
TACAAAGGAGGAATTTTCAC

Downstream 100 bases:

>100_bases
AATAAAACCAATAAATTTTGGAAGTTATTAGCAATCAAATTTGATTTGACCATGTTATCGACTTATATTAAAATTTAATA
CAATTGGTAGGGACCGGGTG

Product: translation elongation factor 2 (EF-2/EF-G)

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 692; Mature: 692

Protein sequence:

>692_residues
MKSYQPDQIRNVGLCSHGGAGKTSLTEALLYSSNSIDRLGNVEDGTTTTDFDKEEVDRGITINTSVAPCEWKGHKLNLVD
TPGYFDFIGDVKSSLRIVDGALIVVCAASGVQVGTEQVWHYADENDLPRVVFINKMERENASFNRTLEQLRENFGDQVVP
VMIPIGSEDSFEGVINLVTMKANYHKGDGKEFSEEEIPSEYQEEADNFREMLMEAVAECDDEMLEKYLEGESLTEDEVIN
GVKQFTKEGELIPVLCGSAIQNIGVTQLMDCLKYYIPSPVDLNYQGFNPETEEEVKINPSEEGPFSAFVFKTVADPYVGR
LNYFRVYSGQLSDDKEAYNSNNKTSERIGKLYYLKGKEQIPVNTVSVGDIAAVSKLQKTATGDTLSDSEKPVKLPEVEFP
DPVISFAVVTQDKGDEEKVGTSLSKFLEEDPILNLEKNDETKETLISGMGELQLEIIIEKMKKKYGVDVELKTPKVPYKE
TITKTVNAEEKYKKQSGGRGQYGHVFIEFSPLQEGEGFQFENNIVGGVIPKTYIPAVEKGIFEAMETGVLAGYPVIDIKA
NLYDGSHHSVDSSEMAFKVAGANAFKKGMQNAQPVLLEPVMEVEVRVPEKFMGDIMGDINSKRGKIQGMDPDGDYQIIKA
YVPQAEMLRYAIDLRSMAQGRGTFTSVFSHYERVPDDVRDEIIEETTAEKEA

Sequences:

>Translated_692_residues
MKSYQPDQIRNVGLCSHGGAGKTSLTEALLYSSNSIDRLGNVEDGTTTTDFDKEEVDRGITINTSVAPCEWKGHKLNLVD
TPGYFDFIGDVKSSLRIVDGALIVVCAASGVQVGTEQVWHYADENDLPRVVFINKMERENASFNRTLEQLRENFGDQVVP
VMIPIGSEDSFEGVINLVTMKANYHKGDGKEFSEEEIPSEYQEEADNFREMLMEAVAECDDEMLEKYLEGESLTEDEVIN
GVKQFTKEGELIPVLCGSAIQNIGVTQLMDCLKYYIPSPVDLNYQGFNPETEEEVKINPSEEGPFSAFVFKTVADPYVGR
LNYFRVYSGQLSDDKEAYNSNNKTSERIGKLYYLKGKEQIPVNTVSVGDIAAVSKLQKTATGDTLSDSEKPVKLPEVEFP
DPVISFAVVTQDKGDEEKVGTSLSKFLEEDPILNLEKNDETKETLISGMGELQLEIIIEKMKKKYGVDVELKTPKVPYKE
TITKTVNAEEKYKKQSGGRGQYGHVFIEFSPLQEGEGFQFENNIVGGVIPKTYIPAVEKGIFEAMETGVLAGYPVIDIKA
NLYDGSHHSVDSSEMAFKVAGANAFKKGMQNAQPVLLEPVMEVEVRVPEKFMGDIMGDINSKRGKIQGMDPDGDYQIIKA
YVPQAEMLRYAIDLRSMAQGRGTFTSVFSHYERVPDDVRDEIIEETTAEKEA
>Mature_692_residues
MKSYQPDQIRNVGLCSHGGAGKTSLTEALLYSSNSIDRLGNVEDGTTTTDFDKEEVDRGITINTSVAPCEWKGHKLNLVD
TPGYFDFIGDVKSSLRIVDGALIVVCAASGVQVGTEQVWHYADENDLPRVVFINKMERENASFNRTLEQLRENFGDQVVP
VMIPIGSEDSFEGVINLVTMKANYHKGDGKEFSEEEIPSEYQEEADNFREMLMEAVAECDDEMLEKYLEGESLTEDEVIN
GVKQFTKEGELIPVLCGSAIQNIGVTQLMDCLKYYIPSPVDLNYQGFNPETEEEVKINPSEEGPFSAFVFKTVADPYVGR
LNYFRVYSGQLSDDKEAYNSNNKTSERIGKLYYLKGKEQIPVNTVSVGDIAAVSKLQKTATGDTLSDSEKPVKLPEVEFP
DPVISFAVVTQDKGDEEKVGTSLSKFLEEDPILNLEKNDETKETLISGMGELQLEIIIEKMKKKYGVDVELKTPKVPYKE
TITKTVNAEEKYKKQSGGRGQYGHVFIEFSPLQEGEGFQFENNIVGGVIPKTYIPAVEKGIFEAMETGVLAGYPVIDIKA
NLYDGSHHSVDSSEMAFKVAGANAFKKGMQNAQPVLLEPVMEVEVRVPEKFMGDIMGDINSKRGKIQGMDPDGDYQIIKA
YVPQAEMLRYAIDLRSMAQGRGTFTSVFSHYERVPDDVRDEIIEETTAEKEA

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=693, Percent_Identity=35.7864357864358, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI19923640, Length=712, Percent_Identity=30.7584269662921, Blast_Score=355, Evalue=7e-98,
Organism=Homo sapiens, GI25306287, Length=712, Percent_Identity=28.6516853932584, Blast_Score=297, Evalue=2e-80,
Organism=Homo sapiens, GI25306283, Length=390, Percent_Identity=35.1282051282051, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI4503483, Length=831, Percent_Identity=23.1046931407942, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI217272892, Length=803, Percent_Identity=23.412204234122, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI217272894, Length=803, Percent_Identity=23.412204234122, Blast_Score=114, Evalue=4e-25,
Organism=Homo sapiens, GI157426893, Length=184, Percent_Identity=31.5217391304348, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=32.8358208955224, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI1789738, Length=690, Percent_Identity=42.463768115942, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI1790835, Length=472, Percent_Identity=25.8474576271186, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=34.2281879194631, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788922, Length=152, Percent_Identity=31.5789473684211, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17533571, Length=692, Percent_Identity=32.514450867052, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17556745, Length=719, Percent_Identity=24.7566063977747, Blast_Score=234, Evalue=8e-62,
Organism=Caenorhabditis elegans, GI17506493, Length=811, Percent_Identity=23.5511713933416, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17552882, Length=813, Percent_Identity=21.5252152521525, Blast_Score=105, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17557151, Length=156, Percent_Identity=34.6153846153846, Blast_Score=89, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323098, Length=692, Percent_Identity=36.9942196531792, Blast_Score=441, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6322359, Length=795, Percent_Identity=27.0440251572327, Blast_Score=287, Evalue=4e-78,
Organism=Saccharomyces cerevisiae, GI6324707, Length=802, Percent_Identity=22.069825436409, Blast_Score=131, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6320593, Length=802, Percent_Identity=22.069825436409, Blast_Score=131, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6323320, Length=153, Percent_Identity=30.0653594771242, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582462, Length=691, Percent_Identity=35.1664254703329, Blast_Score=432, Evalue=1e-121,
Organism=Drosophila melanogaster, GI221458488, Length=712, Percent_Identity=27.8089887640449, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI24585711, Length=816, Percent_Identity=23.5294117647059, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24585713, Length=816, Percent_Identity=23.5294117647059, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24585709, Length=816, Percent_Identity=23.5294117647059, Blast_Score=145, Evalue=9e-35,
Organism=Drosophila melanogaster, GI21357743, Length=423, Percent_Identity=22.6950354609929, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI78706572, Length=182, Percent_Identity=31.8681318681319, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 76997; Mature: 76997

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSYQPDQIRNVGLCSHGGAGKTSLTEALLYSSNSIDRLGNVEDGTTTTDFDKEEVDRGI
CCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCHHHHCCCC
TINTSVAPCEWKGHKLNLVDTPGYFDFIGDVKSSLRIVDGALIVVCAASGVQVGTEQVWH
EEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHE
YADENDLPRVVFINKMERENASFNRTLEQLRENFGDQVVPVMIPIGSEDSFEGVINLVTM
ECCCCCCCEEEEEECHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEE
KANYHKGDGKEFSEEEIPSEYQEEADNFREMLMEAVAECDDEMLEKYLEGESLTEDEVIN
ECCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHH
GVKQFTKEGELIPVLCGSAIQNIGVTQLMDCLKYYIPSPVDLNYQGFNPETEEEVKINPS
HHHHHHHCCCEEEEHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCC
EEGPFSAFVFKTVADPYVGRLNYFRVYSGQLSDDKEAYNSNNKTSERIGKLYYLKGKEQI
CCCCCHHHHHHHHCCCHHCCCEEEEEECCCCCCCHHHHCCCCCHHHHHCEEEEECCCCCC
PVNTVSVGDIAAVSKLQKTATGDTLSDSEKPVKLPEVEFPDPVISFAVVTQDKGDEEKVG
CCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCHHHHH
TSLSKFLEEDPILNLEKNDETKETLISGMGELQLEIIIEKMKKKYGVDVELKTPKVPYKE
HHHHHHHHCCCCEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHH
TITKTVNAEEKYKKQSGGRGQYGHVFIEFSPLQEGEGFQFENNIVGGVIPKTYIPAVEKG
HHHHHCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCCCHHHHHHHHHH
IFEAMETGVLAGYPVIDIKANLYDGSHHSVDSSEMAFKVAGANAFKKGMQNAQPVLLEPV
HHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCHHHEEEEECCHHHHHHHCCCCCEECCHH
MEVEVRVPEKFMGDIMGDINSKRGKIQGMDPDGDYQIIKAYVPQAEMLRYAIDLRSMAQG
HHHEEECCHHHHHHHHHCCCCCCCEEECCCCCCCEEEHEECCCHHHHHHHHHHHHHHHCC
RGTFTSVFSHYERVPDDVRDEIIEETTAEKEA
CCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKSYQPDQIRNVGLCSHGGAGKTSLTEALLYSSNSIDRLGNVEDGTTTTDFDKEEVDRGI
CCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCHHHHCCCC
TINTSVAPCEWKGHKLNLVDTPGYFDFIGDVKSSLRIVDGALIVVCAASGVQVGTEQVWH
EEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHE
YADENDLPRVVFINKMERENASFNRTLEQLRENFGDQVVPVMIPIGSEDSFEGVINLVTM
ECCCCCCCEEEEEECHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEE
KANYHKGDGKEFSEEEIPSEYQEEADNFREMLMEAVAECDDEMLEKYLEGESLTEDEVIN
ECCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHH
GVKQFTKEGELIPVLCGSAIQNIGVTQLMDCLKYYIPSPVDLNYQGFNPETEEEVKINPS
HHHHHHHCCCEEEEHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCC
EEGPFSAFVFKTVADPYVGRLNYFRVYSGQLSDDKEAYNSNNKTSERIGKLYYLKGKEQI
CCCCCHHHHHHHHCCCHHCCCEEEEEECCCCCCCHHHHCCCCCHHHHHCEEEEECCCCCC
PVNTVSVGDIAAVSKLQKTATGDTLSDSEKPVKLPEVEFPDPVISFAVVTQDKGDEEKVG
CCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCHHHHH
TSLSKFLEEDPILNLEKNDETKETLISGMGELQLEIIIEKMKKKYGVDVELKTPKVPYKE
HHHHHHHHCCCCEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHH
TITKTVNAEEKYKKQSGGRGQYGHVFIEFSPLQEGEGFQFENNIVGGVIPKTYIPAVEKG
HHHHHCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCCCHHHHHHHHHH
IFEAMETGVLAGYPVIDIKANLYDGSHHSVDSSEMAFKVAGANAFKKGMQNAQPVLLEPV
HHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCHHHEEEEECCHHHHHHHCCCCCEECCHH
MEVEVRVPEKFMGDIMGDINSKRGKIQGMDPDGDYQIIKAYVPQAEMLRYAIDLRSMAQG
HHHEEECCHHHHHHHHHCCCCCCCEEECCCCCCCEEEHEECCCHHHHHHHHHHHHHHHCC
RGTFTSVFSHYERVPDDVRDEIIEETTAEKEA
CCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA