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The map label for this gene is ddl

Identifier: 188584653

GI number: 188584653

Start: 20325

End: 21422

Strand: Direct

Name: ddl

Synonym: Nther_0011

Alternate gene names: 188584653

Gene position: 20325-21422 (Clockwise)

Preceding gene: 188584652

Following gene: 188584654

Centisome position: 0.64

GC content: 33.97

Gene sequence:

>1098_bases
TTGAAAAAGTTAAGGGTTGGAGTACTTTTTGGAGGACGTTCTGGAGAACATGAAGTTAGCCTAAAGTCTGCGAATTCGAT
TTTGAACGCTTTAGATAGAGAATTATTTGAAGTGATTCCCATAGGAATTCGTAAAAATGGAGAATGGATTTCAGGGGAAG
CACCCTTGGAAGAGTTGGAAACCGGTATAAAAAATCAGGGAAATTATGCTGTAAGTATTCTACCTGATCCATCCAAAAAA
GTTCTTTGGAAATTAGATCCCTTTGAAAAAATTAGTGAGATTGACCTGATTTTCCCAGTGCTTCACGGAACTTTTGGAGA
AGATGGTACTGTTCAAGGTTTTTTAGATCTTTGTGGAATTCCTTATGTGGGTTCAGGAGTTTTAGGATCTTCTTTGGCCA
TGGATAAAGTCATGATGAAAAAAATATTAAGACGTCATGGATTACAAGTTGCTAATTACTATTCATTCAAAAGAAGTGAA
TGGGAACAAGATCGGGATGAAATTATTTTTAGCATAGAAAAACAACTTTCATATCCTATTTTTGTTAAACCTGCTAATTT
AGGGTCTAGTGTTGGAATTTCCAAAGTTAAAAATAGAGAAGAACTAATTCAAGGTATTGATTTAGCTGTTAAATACGATA
TGAAATGTTTAGCCGAAGAATTTATACCAGGAAAAGAAATAGAATTAAGTATCCTTGGTAATGATAATCCTAAGACTTCA
GTTCCAGGAGAGATAATCCCAGCAAATGAGTTTTATGACTACAATGCAAAATATATTAATAATAAAAGCAAACTAGTTAT
CCCAGCACCTCTCTCAGAAACACTTAAGACTAAAATTGAAGACATGGGAGTTGAAGCTTTTAGAGTATTAGACTGTTATG
GATTATCTAGAGTAGATTATTTTGTCCTAGAAGATCAAGAAGAGGTTTATATCAATGAAATCAATACTATGCCTGGCTTT
ACTGAAATTAGCATGTACCCTAAACTATGGACTGAATCGGGACTATCCTACTCTCAACTATTAACTGATTTGATTTATCT
AGCACTAGCAAGGCAGCAAGAAAAGGATAGGAATTCAACTGATTTTTCGGAATCATAA

Upstream 100 bases:

>100_bases
GATGAAATTAAGGAACCTAGAAATGTAATAACGTACTTTGCATAAACTAAATACCATAAGAGACAAAATTTATGTTTTTA
GGTAGTGGAGGTGTTTTAGC

Downstream 100 bases:

>100_bases
CTAAAATAACTTTATATCAATCTTGTTAATGACTAACTATTGTAGTAAAATCAATGTTGAATAGAAAAGGAGGGATTATT
AATGAATATAGAAAAAGGTA

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKKLRVGVLFGGRSGEHEVSLKSANSILNALDRELFEVIPIGIRKNGEWISGEAPLEELETGIKNQGNYAVSILPDPSKK
VLWKLDPFEKISEIDLIFPVLHGTFGEDGTVQGFLDLCGIPYVGSGVLGSSLAMDKVMMKKILRRHGLQVANYYSFKRSE
WEQDRDEIIFSIEKQLSYPIFVKPANLGSSVGISKVKNREELIQGIDLAVKYDMKCLAEEFIPGKEIELSILGNDNPKTS
VPGEIIPANEFYDYNAKYINNKSKLVIPAPLSETLKTKIEDMGVEAFRVLDCYGLSRVDYFVLEDQEEVYINEINTMPGF
TEISMYPKLWTESGLSYSQLLTDLIYLALARQQEKDRNSTDFSES

Sequences:

>Translated_365_residues
MKKLRVGVLFGGRSGEHEVSLKSANSILNALDRELFEVIPIGIRKNGEWISGEAPLEELETGIKNQGNYAVSILPDPSKK
VLWKLDPFEKISEIDLIFPVLHGTFGEDGTVQGFLDLCGIPYVGSGVLGSSLAMDKVMMKKILRRHGLQVANYYSFKRSE
WEQDRDEIIFSIEKQLSYPIFVKPANLGSSVGISKVKNREELIQGIDLAVKYDMKCLAEEFIPGKEIELSILGNDNPKTS
VPGEIIPANEFYDYNAKYINNKSKLVIPAPLSETLKTKIEDMGVEAFRVLDCYGLSRVDYFVLEDQEEVYINEINTMPGF
TEISMYPKLWTESGLSYSQLLTDLIYLALARQQEKDRNSTDFSES
>Mature_365_residues
MKKLRVGVLFGGRSGEHEVSLKSANSILNALDRELFEVIPIGIRKNGEWISGEAPLEELETGIKNQGNYAVSILPDPSKK
VLWKLDPFEKISEIDLIFPVLHGTFGEDGTVQGFLDLCGIPYVGSGVLGSSLAMDKVMMKKILRRHGLQVANYYSFKRSE
WEQDRDEIIFSIEKQLSYPIFVKPANLGSSVGISKVKNREELIQGIDLAVKYDMKCLAEEFIPGKEIELSILGNDNPKTS
VPGEIIPANEFYDYNAKYINNKSKLVIPAPLSETLKTKIEDMGVEAFRVLDCYGLSRVDYFVLEDQEEVYINEINTMPGF
TEISMYPKLWTESGLSYSQLLTDLIYLALARQQEKDRNSTDFSES

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=359, Percent_Identity=45.125348189415, Blast_Score=314, Evalue=7e-87,
Organism=Escherichia coli, GI1786280, Length=345, Percent_Identity=33.0434782608696, Blast_Score=181, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_NATTJ (B2A2Z6)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001916198.1
- ProteinModelPortal:   B2A2Z6
- SMR:   B2A2Z6
- GeneID:   6314408
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_0011
- HOGENOM:   HBG427092
- OMA:   CANREEL
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 41091; Mature: 41091

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLRVGVLFGGRSGEHEVSLKSANSILNALDRELFEVIPIGIRKNGEWISGEAPLEELE
CCCEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCEECCCCEECCCCCHHHHH
TGIKNQGNYAVSILPDPSKKVLWKLDPFEKISEIDLIFPVLHGTFGEDGTVQGFLDLCGI
HCCCCCCCEEEEEECCCCHHEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCC
PYVGSGVLGSSLAMDKVMMKKILRRHGLQVANYYSFKRSEWEQDRDEIIFSIEKQLSYPI
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCHHHHHHHHHHHCCCCE
FVKPANLGSSVGISKVKNREELIQGIDLAVKYDMKCLAEEFIPGKEIELSILGNDNPKTS
EEEECCCCCCCCHHHHCCHHHHHHHHHHEEHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
VPGEIIPANEFYDYNAKYINNKSKLVIPAPLSETLKTKIEDMGVEAFRVLDCYGLSRVDY
CCCCEEECCCCCCCCCEEECCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEE
FVLEDQEEVYINEINTMPGFTEISMYPKLWTESGLSYSQLLTDLIYLALARQQEKDRNST
EEECCCCCEEEEECCCCCCCEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCC
DFSES
CCCCC
>Mature Secondary Structure
MKKLRVGVLFGGRSGEHEVSLKSANSILNALDRELFEVIPIGIRKNGEWISGEAPLEELE
CCCEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCEECCCCEECCCCCHHHHH
TGIKNQGNYAVSILPDPSKKVLWKLDPFEKISEIDLIFPVLHGTFGEDGTVQGFLDLCGI
HCCCCCCCEEEEEECCCCHHEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCC
PYVGSGVLGSSLAMDKVMMKKILRRHGLQVANYYSFKRSEWEQDRDEIIFSIEKQLSYPI
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCHHHHHHHHHHHCCCCE
FVKPANLGSSVGISKVKNREELIQGIDLAVKYDMKCLAEEFIPGKEIELSILGNDNPKTS
EEEECCCCCCCCHHHHCCHHHHHHHHHHEEHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
VPGEIIPANEFYDYNAKYINNKSKLVIPAPLSETLKTKIEDMGVEAFRVLDCYGLSRVDY
CCCCEEECCCCCCCCCEEECCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEE
FVLEDQEEVYINEINTMPGFTEISMYPKLWTESGLSYSQLLTDLIYLALARQQEKDRNST
EEECCCCCEEEEECCCCCCCEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCC
DFSES
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA