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The map label for this gene is pcm2 [H]

Identifier: 187927743

GI number: 187927743

Start: 687036

End: 687686

Strand: Reverse

Name: pcm2 [H]

Synonym: Rpic_0647

Alternate gene names: 187927743

Gene position: 687686-687036 (Counterclockwise)

Preceding gene: 187927744

Following gene: 187927742

Centisome position: 17.44

GC content: 64.82

Gene sequence:

>651_bases
ATGGACATCGAAAAATCCCGGTTCAACATGATCGAGCAGCAAATCCGTCCTTGGGACGTGCTCGATACCGACGTTCTCGA
CCTGCTCAGCGTCGTCAAGCGCGAACACTACGTGCCCGAGGCCTACCGCGCGCTGGCCTTCGTCGACATGGAAATCCCGC
TGGCCGGCGGCCAGAACATGCTGGCACCGCGCGTTGAAGCTCGCGTGCTGCAGGAGCTGGCCGTGCGCAAGCACGAAGAC
GTGCTGGAAGTCGGCGCGGGCTCCGGCTACATGGCCGCCCTGCTGGCCCATCGCGGCCGCCAGGTCACCACGCTCGACAT
CGTGCCGGAACTCGTTGCCTTCGCCCGTGACAATCTCTCGCGCAATAGCGTCACTAACGTCGACGTGGTCGAGGCCAGCG
GCGCGCAAGGCTGGGGCGACGGCCTGTATGACGTCATCTGCGTGTCGGGCTCGGTGCCCGCCGTGCCGGAAGCGCTGCTC
AAGCAGCTCAAGGTGGGTGGTCGCCTGTCGATCTTCGTGGGCGGCGCGCCGGTCATGGAAGCGCAGCTCATCACGCGCAT
CTCCAACACCGAATATCAGACGCGCAACCTGTTCGAGACGTATGTGACGCCGCTGGTGGGTGTTCCCGCGCCGTCGTCCT
TCCGCTTCTGA

Upstream 100 bases:

>100_bases
CATGACAACGTCTGCCGGAATGTCTTCCGGACTTGCTAAAATGCCGGGTTTGTCGTCAAACCCTCATTCGCATCACTCGC
TCCGCGTGGAAGGCATGCCC

Downstream 100 bases:

>100_bases
TCCCTCTCGCCCCCCAATCATCATGCAGCAGCTCGCTCCCACCGCCCTCGCCCAATGGCTCGCCGACGCATCGCGCGCGA
AGCCCGTCCTGCTCGACGTG

Product: Protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 2; Protein L-isoaspartyl methyltransferase 2; Protein-beta-aspartate methyltransferase 2; PIMT 2 [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MDIEKSRFNMIEQQIRPWDVLDTDVLDLLSVVKREHYVPEAYRALAFVDMEIPLAGGQNMLAPRVEARVLQELAVRKHED
VLEVGAGSGYMAALLAHRGRQVTTLDIVPELVAFARDNLSRNSVTNVDVVEASGAQGWGDGLYDVICVSGSVPAVPEALL
KQLKVGGRLSIFVGGAPVMEAQLITRISNTEYQTRNLFETYVTPLVGVPAPSSFRF

Sequences:

>Translated_216_residues
MDIEKSRFNMIEQQIRPWDVLDTDVLDLLSVVKREHYVPEAYRALAFVDMEIPLAGGQNMLAPRVEARVLQELAVRKHED
VLEVGAGSGYMAALLAHRGRQVTTLDIVPELVAFARDNLSRNSVTNVDVVEASGAQGWGDGLYDVICVSGSVPAVPEALL
KQLKVGGRLSIFVGGAPVMEAQLITRISNTEYQTRNLFETYVTPLVGVPAPSSFRF
>Mature_216_residues
MDIEKSRFNMIEQQIRPWDVLDTDVLDLLSVVKREHYVPEAYRALAFVDMEIPLAGGQNMLAPRVEARVLQELAVRKHED
VLEVGAGSGYMAALLAHRGRQVTTLDIVPELVAFARDNLSRNSVTNVDVVEASGAQGWGDGLYDVICVSGSVPAVPEALL
KQLKVGGRLSIFVGGAPVMEAQLITRISNTEYQTRNLFETYVTPLVGVPAPSSFRF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=169, Percent_Identity=34.3195266272189, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23710; Mature: 23710

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIEKSRFNMIEQQIRPWDVLDTDVLDLLSVVKREHYVPEAYRALAFVDMEIPLAGGQNM
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEECCCCCCC
LAPRVEARVLQELAVRKHEDVLEVGAGSGYMAALLAHRGRQVTTLDIVPELVAFARDNLS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCC
RNSVTNVDVVEASGAQGWGDGLYDVICVSGSVPAVPEALLKQLKVGGRLSIFVGGAPVME
CCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCHHH
AQLITRISNTEYQTRNLFETYVTPLVGVPAPSSFRF
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDIEKSRFNMIEQQIRPWDVLDTDVLDLLSVVKREHYVPEAYRALAFVDMEIPLAGGQNM
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEECCCCCCC
LAPRVEARVLQELAVRKHEDVLEVGAGSGYMAALLAHRGRQVTTLDIVPELVAFARDNLS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCC
RNSVTNVDVVEASGAQGWGDGLYDVICVSGSVPAVPEALLKQLKVGGRLSIFVGGAPVME
CCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCHHH
AQLITRISNTEYQTRNLFETYVTPLVGVPAPSSFRF
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA