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The map label for this gene is rfbB [H]

Identifier: 187927736

GI number: 187927736

Start: 679332

End: 680393

Strand: Reverse

Name: rfbB [H]

Synonym: Rpic_0640

Alternate gene names: 187927736

Gene position: 680393-679332 (Counterclockwise)

Preceding gene: 187927741

Following gene: 187927735

Centisome position: 17.26

GC content: 58.57

Gene sequence:

>1062_bases
ATGATTTTGATTACAGGCGGTGCTGGTTTTATCGGCGCCAATTTCGTTCTGGACTGGCTGCGTGGTTCCGATGAGCCGGT
GGTCAATGTCGACAAACTGACCTATGCTGGCAATCTGAAGACCCTCGCGTCGTTGGAGGGAGATGCCCGGCATACGTTCG
TGCGCGCGGACATTTGTGATCGCGCGTCGATGGACGAGCTGCTGACCCGACACGCACCACGGGCGATCGTGCATTTTGCG
GCAGAAAGCCACGTGGACCGGTCAATCCACGGCCCGGCGGATTTCGTGCAAACGAACGTGTTCGGAACGTTCACGCTGCT
GGAAGCTGCGCGCGCCTACTGGAACCGGCTAGAGGGCGCGGCACGCGCAAATTTCCGCTTCCATCACGTTTCCACAGATG
AGGTCTATGGCTCCCTGGGGCCGGACGACGCCCCCTTTACGGAAACCACGCCCTACGCCCCCAACAGCCCCTATTCGGCT
TCGAAAGCGGGCTCGGACCATTTGGTTCGCGCGTACTTCCATACTTATGGGCTGCCGGTTCTTACGACCAACTGCTCGAA
TAACTACGGCCCGTATCACTTCCCCGAGAAGCTCATCCCTCTCATGATCGCCAATGCGTTGGCGGGCAAGCCCCTGCCCG
TGTATGGCGATGGTCTAAACGTACGGGATTGGCTCTACGTCGGCGACCACTGTGCCGCGATCCGACGCGTGCTGGAAGCG
GGCACGGTCGGCGAGGTCTACAACGTGGGCGGCTGGAATGAGAAGAACAATCTTGAAGTCGTTCATACGCTTTGTGACCT
GCTCGATACCCTTAAACCGCGCGCCCACGGCTCATATCGCGAACAAATTACTTTCGTAAGAGACCGCCCCGGCCATGACC
GACGCTACGCGATCGACGCACGAAAACTCGAGCGTGAGTTGAATTGGAAACCGGCAGAAACGTTCGAGACTGGCCTGCGC
AAAACCGTCGAGTGGTACCTCGAGAATCAGGAATGGGTCGCGGATGTGATGTCTGGCGCCTATCGGGATTGGGTTGATGT
GAACTACGGTAGCCGCCGCTAA

Upstream 100 bases:

>100_bases
ATAATACTTGGCTGGCTTGTATGAGCCGTCCGACTCTTCCCCCACTGCGCAGCCATTCTTTGACTTCAGCCTCTGCGCGC
AACGTTCACCCGACAATCGC

Downstream 100 bases:

>100_bases
CAACCGGACTTTTCATGGCACGCAAAGGCATTATTCTAGCAGGTGGCTCCGGGACACGGCTGTATCCCATCACGCACTCT
GTGTCGAAGCAATTGCTTCC

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MILITGGAGFIGANFVLDWLRGSDEPVVNVDKLTYAGNLKTLASLEGDARHTFVRADICDRASMDELLTRHAPRAIVHFA
AESHVDRSIHGPADFVQTNVFGTFTLLEAARAYWNRLEGAARANFRFHHVSTDEVYGSLGPDDAPFTETTPYAPNSPYSA
SKAGSDHLVRAYFHTYGLPVLTTNCSNNYGPYHFPEKLIPLMIANALAGKPLPVYGDGLNVRDWLYVGDHCAAIRRVLEA
GTVGEVYNVGGWNEKNNLEVVHTLCDLLDTLKPRAHGSYREQITFVRDRPGHDRRYAIDARKLERELNWKPAETFETGLR
KTVEWYLENQEWVADVMSGAYRDWVDVNYGSRR

Sequences:

>Translated_353_residues
MILITGGAGFIGANFVLDWLRGSDEPVVNVDKLTYAGNLKTLASLEGDARHTFVRADICDRASMDELLTRHAPRAIVHFA
AESHVDRSIHGPADFVQTNVFGTFTLLEAARAYWNRLEGAARANFRFHHVSTDEVYGSLGPDDAPFTETTPYAPNSPYSA
SKAGSDHLVRAYFHTYGLPVLTTNCSNNYGPYHFPEKLIPLMIANALAGKPLPVYGDGLNVRDWLYVGDHCAAIRRVLEA
GTVGEVYNVGGWNEKNNLEVVHTLCDLLDTLKPRAHGSYREQITFVRDRPGHDRRYAIDARKLERELNWKPAETFETGLR
KTVEWYLENQEWVADVMSGAYRDWVDVNYGSRR
>Mature_353_residues
MILITGGAGFIGANFVLDWLRGSDEPVVNVDKLTYAGNLKTLASLEGDARHTFVRADICDRASMDELLTRHAPRAIVHFA
AESHVDRSIHGPADFVQTNVFGTFTLLEAARAYWNRLEGAARANFRFHHVSTDEVYGSLGPDDAPFTETTPYAPNSPYSA
SKAGSDHLVRAYFHTYGLPVLTTNCSNNYGPYHFPEKLIPLMIANALAGKPLPVYGDGLNVRDWLYVGDHCAAIRRVLEA
GTVGEVYNVGGWNEKNNLEVVHTLCDLLDTLKPRAHGSYREQITFVRDRPGHDRRYAIDARKLERELNWKPAETFETGLR
KTVEWYLENQEWVADVMSGAYRDWVDVNYGSRR

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=332, Percent_Identity=36.4457831325301, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI42516563, Length=341, Percent_Identity=25.5131964809384, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI56237023, Length=297, Percent_Identity=27.6094276094276, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI56118217, Length=297, Percent_Identity=27.6094276094276, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI189083684, Length=297, Percent_Identity=27.6094276094276, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4504031, Length=296, Percent_Identity=23.9864864864865, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788353, Length=360, Percent_Identity=60.8333333333333, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=63.3720930232558, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1786974, Length=355, Percent_Identity=29.0140845070423, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788366, Length=179, Percent_Identity=28.4916201117318, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17568069, Length=333, Percent_Identity=36.036036036036, Blast_Score=208, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI115532424, Length=337, Percent_Identity=33.2344213649852, Blast_Score=181, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI71982035, Length=368, Percent_Identity=26.3586956521739, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17539532, Length=334, Percent_Identity=24.8502994011976, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=370, Percent_Identity=26.2162162162162, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI133901788, Length=197, Percent_Identity=27.4111675126904, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=197, Percent_Identity=27.4111675126904, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=291, Percent_Identity=24.0549828178694, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=197, Percent_Identity=27.4111675126904, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=197, Percent_Identity=27.4111675126904, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=197, Percent_Identity=27.4111675126904, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=364, Percent_Identity=25.8241758241758, Blast_Score=89, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21356223, Length=335, Percent_Identity=27.1641791044776, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19923002, Length=366, Percent_Identity=27.3224043715847, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24158427, Length=340, Percent_Identity=25, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39633; Mature: 39633

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILITGGAGFIGANFVLDWLRGSDEPVVNVDKLTYAGNLKTLASLEGDARHTFVRADICD
CEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHCCCCCHHEEEEHHHHC
RASMDELLTRHAPRAIVHFAAESHVDRSIHGPADFVQTNVFGTFTLLEAARAYWNRLEGA
CCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ARANFRFHHVSTDEVYGSLGPDDAPFTETTPYAPNSPYSASKAGSDHLVRAYFHTYGLPV
HHCCEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCE
LTTNCSNNYGPYHFPEKLIPLMIANALAGKPLPVYGDGLNVRDWLYVGDHCAAIRRVLEA
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEECHHHHHHHHHHHC
GTVGEVYNVGGWNEKNNLEVVHTLCDLLDTLKPRAHGSYREQITFVRDRPGHDRRYAIDA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHEEEEECCCCCCCEEEHHH
RKLERELNWKPAETFETGLRKTVEWYLENQEWVADVMSGAYRDWVDVNYGSRR
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHCCEECCCCCCC
>Mature Secondary Structure
MILITGGAGFIGANFVLDWLRGSDEPVVNVDKLTYAGNLKTLASLEGDARHTFVRADICD
CEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHCCCCCHHEEEEHHHHC
RASMDELLTRHAPRAIVHFAAESHVDRSIHGPADFVQTNVFGTFTLLEAARAYWNRLEGA
CCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ARANFRFHHVSTDEVYGSLGPDDAPFTETTPYAPNSPYSASKAGSDHLVRAYFHTYGLPV
HHCCEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCE
LTTNCSNNYGPYHFPEKLIPLMIANALAGKPLPVYGDGLNVRDWLYVGDHCAAIRRVLEA
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEECHHHHHHHHHHHC
GTVGEVYNVGGWNEKNNLEVVHTLCDLLDTLKPRAHGSYREQITFVRDRPGHDRRYAIDA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHEEEEECCCCCCCEEEHHH
RKLERELNWKPAETFETGLRKTVEWYLENQEWVADVMSGAYRDWVDVNYGSRR
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609; 11796113 [H]