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The map label for this gene is tolC [H]

Identifier: 187927741

GI number: 187927741

Start: 685099

End: 686565

Strand: Reverse

Name: tolC [H]

Synonym: Rpic_0645

Alternate gene names: 187927741

Gene position: 686565-685099 (Counterclockwise)

Preceding gene: 187927742

Following gene: 187927736

Centisome position: 17.41

GC content: 64.76

Gene sequence:

>1467_bases
ATGACTGTTGCTCGCAGTAGCCGCACGCGGATAGCCGCGCGCGGTTCGCTTGTTGTGCTGTGCTGTTCGATGATGCCGAT
GTTCGCGTGGGCGCAGACCCAACCGAACACGGCCGCTGCAACCGACCTCCTCTCCGCCTACCGCGCCGCGCTGGCCAACG
ATCCGCAATTCGCATCGGCGCGCGCGCAGTCGCTGGCGCAACGCGAGAAGCTCACGCAAGGCCGCGCTGGTTTGCTACCG
CAGATCGGCGGCGCCTGGAGCTCCACGCGCATCATCTTCGATCAAACCGCTCCGGCCGAATTCAACAAGACGTTCTCTTC
GACGGGCTGGACGCTGTCACTGTCGCAGCCGCTCTTCCGCTGGGACCGCTGGGAAACCTACAAGCAAGGCGAGATTGCCG
CGCAGGCCGCCGAGGCTTCGCTCGCGCAGGCGCAGCTCGACCTCATCACACGCACGGCGCGGGCCTACTTTGACGTGCTC
AACGCGCAGGACACGCTGCAGCTCGCCACCGCGCAGCGCGATGCGATCCAGCAGCAGTACGAACAGGCTCGCCGCAACTT
CGACGTCGGCAACGCCAACATCACCGACGCGAACGACGCACAGGCCCGCCGCGACGCGATCGACGCCAGCGTCATCGCAG
CACGCAGCGACTTTGAGATCAAGCAATCGGCGCTCAAGCAGATCATCGGGGAGCCCGTTGGCACGCTGGCCGGCGTGCGC
ACGGGCGTCACGATCCCCCAGCCGGAACCGGCTTCAGCAGAACCCTGGGTGGAACAGGCGCGCAGCAGCAACCTGCAGGT
GGCACTGGCGCAATACAACCTGCAAAGTGCAGAGCGCGATCTGAAGAAGGCCACCGCGGGCCATCTGCCTTCGGTCGACC
TCGTGGCGCAGACCGGCCACACCAATGCCAGCGGCAACCAATACCTGCCGTCGCTGCCCGGCGGCGCGCGCTTCGACAGT
TCACAGATTGGCGTGCAGGTGTCGATCCCGATCTTTGCAGGCGGTGCGATCCAGTCGCGCGTGCGTGAAAACATCGCGCT
GGAAACCAAGGCCGCAAACGATCTTGAATTTGCCCGGCGCTCTGCCGAACAAGGCGCACGGCAAGCCTATATCGGCGTGA
TTGCCGGATTGGCGCAGGTCAAGGCACTGGAAGCGGCCGAGCTGTCGGCACGTACAGCGTACGACTCCAACCAGCTCGCC
TACGGTGTGGGCGTGCGCATCGGCACCGACGTGCTGAATTCGCAGGCGCAGCTGTTTCAGGCGCGGCGCGATCTGGCACG
CGCGCGTTATGACACCATCGTCAACGGGCTGCGGCTGAAGTCTGCAGCCGGCTCGTTGACTGAGGGAGACGTGATCCAGG
TGAACACGCTGCTCACGGCCAACGGTTCGAGCGATATCACTGCGTTGCCGCCGGCAAGCACCAAACTTGAATCGGCGAAA
CCAACGGGCAAATCGGCCAAACGCTGA

Upstream 100 bases:

>100_bases
CCGGGCAAAGCCCTGTTCACGCTCCACAGGCACTGAACAGGGCCTGACGCGCCCGCGCTGCCTTCCGAACGCGAACGTTT
CCTCCTCGCTCGGGGGCTTT

Downstream 100 bases:

>100_bases
TCAATGTGTCTGGCCGCGCGGCGGCAGCGGGCGCTCGGCAAACTGCAGCAGCGCGCCGCTGGTCGTTTTCCAGATAACCT
GGCGGCCCTGCGTGCCGCTG

Product: type I secretion outer membrane protein, TolC family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTVARSSRTRIAARGSLVVLCCSMMPMFAWAQTQPNTAAATDLLSAYRAALANDPQFASARAQSLAQREKLTQGRAGLLP
QIGGAWSSTRIIFDQTAPAEFNKTFSSTGWTLSLSQPLFRWDRWETYKQGEIAAQAAEASLAQAQLDLITRTARAYFDVL
NAQDTLQLATAQRDAIQQQYEQARRNFDVGNANITDANDAQARRDAIDASVIAARSDFEIKQSALKQIIGEPVGTLAGVR
TGVTIPQPEPASAEPWVEQARSSNLQVALAQYNLQSAERDLKKATAGHLPSVDLVAQTGHTNASGNQYLPSLPGGARFDS
SQIGVQVSIPIFAGGAIQSRVRENIALETKAANDLEFARRSAEQGARQAYIGVIAGLAQVKALEAAELSARTAYDSNQLA
YGVGVRIGTDVLNSQAQLFQARRDLARARYDTIVNGLRLKSAAGSLTEGDVIQVNTLLTANGSSDITALPPASTKLESAK
PTGKSAKR

Sequences:

>Translated_488_residues
MTVARSSRTRIAARGSLVVLCCSMMPMFAWAQTQPNTAAATDLLSAYRAALANDPQFASARAQSLAQREKLTQGRAGLLP
QIGGAWSSTRIIFDQTAPAEFNKTFSSTGWTLSLSQPLFRWDRWETYKQGEIAAQAAEASLAQAQLDLITRTARAYFDVL
NAQDTLQLATAQRDAIQQQYEQARRNFDVGNANITDANDAQARRDAIDASVIAARSDFEIKQSALKQIIGEPVGTLAGVR
TGVTIPQPEPASAEPWVEQARSSNLQVALAQYNLQSAERDLKKATAGHLPSVDLVAQTGHTNASGNQYLPSLPGGARFDS
SQIGVQVSIPIFAGGAIQSRVRENIALETKAANDLEFARRSAEQGARQAYIGVIAGLAQVKALEAAELSARTAYDSNQLA
YGVGVRIGTDVLNSQAQLFQARRDLARARYDTIVNGLRLKSAAGSLTEGDVIQVNTLLTANGSSDITALPPASTKLESAK
PTGKSAKR
>Mature_487_residues
TVARSSRTRIAARGSLVVLCCSMMPMFAWAQTQPNTAAATDLLSAYRAALANDPQFASARAQSLAQREKLTQGRAGLLPQ
IGGAWSSTRIIFDQTAPAEFNKTFSSTGWTLSLSQPLFRWDRWETYKQGEIAAQAAEASLAQAQLDLITRTARAYFDVLN
AQDTLQLATAQRDAIQQQYEQARRNFDVGNANITDANDAQARRDAIDASVIAARSDFEIKQSALKQIIGEPVGTLAGVRT
GVTIPQPEPASAEPWVEQARSSNLQVALAQYNLQSAERDLKKATAGHLPSVDLVAQTGHTNASGNQYLPSLPGGARFDSS
QIGVQVSIPIFAGGAIQSRVRENIALETKAANDLEFARRSAEQGARQAYIGVIAGLAQVKALEAAELSARTAYDSNQLAY
GVGVRIGTDVLNSQAQLFQARRDLARARYDTIVNGLRLKSAAGSLTEGDVIQVNTLLTANGSSDITALPPASTKLESAKP
TGKSAKR

Specific function: May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=449, Percent_Identity=31.1804008908686, Blast_Score=200, Evalue=1e-52,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52205; Mature: 52074

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVARSSRTRIAARGSLVVLCCSMMPMFAWAQTQPNTAAATDLLSAYRAALANDPQFASA
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
RAQSLAQREKLTQGRAGLLPQIGGAWSSTRIIFDQTAPAEFNKTFSSTGWTLSLSQPLFR
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCEEECCCHHHH
WDRWETYKQGEIAAQAAEASLAQAQLDLITRTARAYFDVLNAQDTLQLATAQRDAIQQQY
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EQARRNFDVGNANITDANDAQARRDAIDASVIAARSDFEIKQSALKQIIGEPVGTLAGVR
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHH
TGVTIPQPEPASAEPWVEQARSSNLQVALAQYNLQSAERDLKKATAGHLPSVDLVAQTGH
CCCCCCCCCCCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEECCC
TNASGNQYLPSLPGGARFDSSQIGVQVSIPIFAGGAIQSRVRENIALETKAANDLEFARR
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECHHHHHHHHHHHEEECCCCCHHHHHHH
SAEQGARQAYIGVIAGLAQVKALEAAELSARTAYDSNQLAYGVGVRIGTDVLNSQAQLFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEECHHHHHHHHHHHH
ARRDLARARYDTIVNGLRLKSAAGSLTEGDVIQVNTLLTANGSSDITALPPASTKLESAK
HHHHHHHHHHHHHHHHHHEHHCCCCCCCCCEEEEEEEEEECCCCCEEECCCCCCCCCCCC
PTGKSAKR
CCCCCCCC
>Mature Secondary Structure 
TVARSSRTRIAARGSLVVLCCSMMPMFAWAQTQPNTAAATDLLSAYRAALANDPQFASA
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
RAQSLAQREKLTQGRAGLLPQIGGAWSSTRIIFDQTAPAEFNKTFSSTGWTLSLSQPLFR
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCEEECCCHHHH
WDRWETYKQGEIAAQAAEASLAQAQLDLITRTARAYFDVLNAQDTLQLATAQRDAIQQQY
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EQARRNFDVGNANITDANDAQARRDAIDASVIAARSDFEIKQSALKQIIGEPVGTLAGVR
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHH
TGVTIPQPEPASAEPWVEQARSSNLQVALAQYNLQSAERDLKKATAGHLPSVDLVAQTGH
CCCCCCCCCCCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEECCC
TNASGNQYLPSLPGGARFDSSQIGVQVSIPIFAGGAIQSRVRENIALETKAANDLEFARR
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECHHHHHHHHHHHEEECCCCCHHHHHHH
SAEQGARQAYIGVIAGLAQVKALEAAELSARTAYDSNQLAYGVGVRIGTDVLNSQAQLFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEECHHHHHHHHHHHH
ARRDLARARYDTIVNGLRLKSAAGSLTEGDVIQVNTLLTANGSSDITALPPASTKLESAK
HHHHHHHHHHHHHHHHHHEHHCCCCCCCCCEEEEEEEEEECCCCCEEECCCCCCCCCCCC
PTGKSAKR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]