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The map label for this gene is proP [H]

Identifier: 187927563

GI number: 187927563

Start: 495263

End: 496966

Strand: Reverse

Name: proP [H]

Synonym: Rpic_0463

Alternate gene names: 187927563

Gene position: 496966-495263 (Counterclockwise)

Preceding gene: 187927568

Following gene: 187927562

Centisome position: 12.61

GC content: 63.56

Gene sequence:

>1704_bases
ATGGCAACCGTGCAGAGTGCACCGAACGCGCCCGTCGGGCGTGTGCAGCCCGCGCCCATGACGAGGGAGGAGAAGAAGGT
CATCTTTGCCTCGTCGCTGGGGACGGTGTTCGAGTGGTACGACTTTTATCTTTACGGCTCGTTGGCGGCCATCATCGCCA
AGCAGTTCTTCTCGGGCCTGGACCCCACGGCGGGTTTCATCTTTGCCCTGCTGGCCTTTGCCGCCGGCTTCCTGGTGCGC
CCGTTCGGCGCGCTGGTGTTCGGCCGTCTGGGCGACATGATCGGGCGCAAGTACACGTTCCTGGTCACCATCGTCATCAT
GGGTACGTCGACGTTCATCGTCGGCCTGCTTCCGTCGTACGGCACGATTGGCGTTGCTGCGCCGGTCATCCTGATCGCGC
TGCGCATGCTGCAAGGTCTGGCACTGGGCGGTGAGTACGGCGGCGCCGCCACGTATGTCGCCGAGCACGCCCCGCACGGC
CGCCGCGGTGCCTACACATCGTGGATCCAGACGACGGCTACGTTGGGCCTGTTCCTCTCGCTGGTCGTCATCCTGGCCGT
GCGCGAACTGACCGGCAAGGCGTTCGACGACTGGGGCTGGCGCATCCCGTTCCTGGTCTCGATCCTGCTGCTCGCCACGT
CGGTGTACATCCGCCTGTCGATGAGCGAATCGCCCGCATTCCAGAAGATGAAGGCCGAAGGCAAGACCTCCAAGGCCCCG
TTGACAGAAGCATTCGGCCAATGGCGCAACCTGAAGATCGTCATCCTGGCGCTGCTCGGCCTGACCGCCGGCCAGGCTGT
GGTGTGGTACACGGGCCAGTTCTACGCACTGTTCTTCCTCACGCAGGTGCTGAAGGTCGACGCATTCACGGCCAACGTGC
TGATTGCGGTGGCGCTTGCCATCGGCACGCCGTTCTTCGTGTTCTTCGGCTCGCTGTCGGACCGCATCGGTCGCAAGAAG
ATCATCATGGCCGGCTGCCTGCTGGCGGTGCTGACCTACTTCCCGCTGTTCAAGGCGCTCACGCACTACGCCAACCCGGC
GCTGGAGCGCGCCCAGCAGACCGCGCAGATCACCATCACGGTCGATCCGAAGGAGTGCTCGTTCCAGGGCAGCCCGATCG
CCCGCGAGGTGGACTTCCGCTCGTCGTGCGACATCGCCAAGCGCACGCTGGCGCAGTCGTCGGCCAGCTACGAGACGGTC
GAAGGCCCGGCCGGCACGGTCGCATCGGTGTCGATCGCGGGTAAGCAGATCACCTCGCTCAACGCCACCCGCACGGCAGA
CGGCCAGAACTTCGACGCCGACAGCAAGGCCAAGATCGCCGACTTCAAGAAGCAGGTGAGCGAGTCGCTCAAGGCGGCCA
ACTACCCGGCCAAGGCCGATCCCGCCCAGATGAACACGATCATGGTGCTGGTGATCCTGGTGATCCTGGTGATCTACGTG
ACCATGGTCTACGGCCCGATCGCGGCAATGCTGGTGGAGATGTTCCCGACGCGCATCCGGTACTCGTCGATGTCGCTGCC
GTATCACATCGGCAACGGCTGGTTTGGGGGCCTGCTGCCGACCATTTCGTTCGCCCTCGTGGCCCAGAACGGCAATATCT
ATCACGGTTTGTGGTACCCGATCTGGATCGCGGCCATCACCTTCGTGATCGGTGTGCTGTTCGTGCGCGAGACCAAGGAT
GTGGATATTTATCGCAACGACTAA

Upstream 100 bases:

>100_bases
TTCGGCTTCCACAATCGGTGACACTTCCGCGGGCGAAAAGCGTTTGCGTTGTCATGTCGGTGCCCAGCGGGACGACCTAC
GAAAAAAAACAGGAGACAAC

Downstream 100 bases:

>100_bases
CGCAGGGGGTTGACATATTCGCCGAAGACGGTAGAATCCCGCTTCTTCGGCGAATTAGCTCAGTCGGTTAGAGCGACGGA
ATCATAATCCGCAGGTCCGG

Product: major facilitator superfamily protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MATVQSAPNAPVGRVQPAPMTREEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFSGLDPTAGFIFALLAFAAGFLVR
PFGALVFGRLGDMIGRKYTFLVTIVIMGTSTFIVGLLPSYGTIGVAAPVILIALRMLQGLALGGEYGGAATYVAEHAPHG
RRGAYTSWIQTTATLGLFLSLVVILAVRELTGKAFDDWGWRIPFLVSILLLATSVYIRLSMSESPAFQKMKAEGKTSKAP
LTEAFGQWRNLKIVILALLGLTAGQAVVWYTGQFYALFFLTQVLKVDAFTANVLIAVALAIGTPFFVFFGSLSDRIGRKK
IIMAGCLLAVLTYFPLFKALTHYANPALERAQQTAQITITVDPKECSFQGSPIAREVDFRSSCDIAKRTLAQSSASYETV
EGPAGTVASVSIAGKQITSLNATRTADGQNFDADSKAKIADFKKQVSESLKAANYPAKADPAQMNTIMVLVILVILVIYV
TMVYGPIAAMLVEMFPTRIRYSSMSLPYHIGNGWFGGLLPTISFALVAQNGNIYHGLWYPIWIAAITFVIGVLFVRETKD
VDIYRND

Sequences:

>Translated_567_residues
MATVQSAPNAPVGRVQPAPMTREEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFSGLDPTAGFIFALLAFAAGFLVR
PFGALVFGRLGDMIGRKYTFLVTIVIMGTSTFIVGLLPSYGTIGVAAPVILIALRMLQGLALGGEYGGAATYVAEHAPHG
RRGAYTSWIQTTATLGLFLSLVVILAVRELTGKAFDDWGWRIPFLVSILLLATSVYIRLSMSESPAFQKMKAEGKTSKAP
LTEAFGQWRNLKIVILALLGLTAGQAVVWYTGQFYALFFLTQVLKVDAFTANVLIAVALAIGTPFFVFFGSLSDRIGRKK
IIMAGCLLAVLTYFPLFKALTHYANPALERAQQTAQITITVDPKECSFQGSPIAREVDFRSSCDIAKRTLAQSSASYETV
EGPAGTVASVSIAGKQITSLNATRTADGQNFDADSKAKIADFKKQVSESLKAANYPAKADPAQMNTIMVLVILVILVIYV
TMVYGPIAAMLVEMFPTRIRYSSMSLPYHIGNGWFGGLLPTISFALVAQNGNIYHGLWYPIWIAAITFVIGVLFVRETKD
VDIYRND
>Mature_566_residues
ATVQSAPNAPVGRVQPAPMTREEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFSGLDPTAGFIFALLAFAAGFLVRP
FGALVFGRLGDMIGRKYTFLVTIVIMGTSTFIVGLLPSYGTIGVAAPVILIALRMLQGLALGGEYGGAATYVAEHAPHGR
RGAYTSWIQTTATLGLFLSLVVILAVRELTGKAFDDWGWRIPFLVSILLLATSVYIRLSMSESPAFQKMKAEGKTSKAPL
TEAFGQWRNLKIVILALLGLTAGQAVVWYTGQFYALFFLTQVLKVDAFTANVLIAVALAIGTPFFVFFGSLSDRIGRKKI
IMAGCLLAVLTYFPLFKALTHYANPALERAQQTAQITITVDPKECSFQGSPIAREVDFRSSCDIAKRTLAQSSASYETVE
GPAGTVASVSIAGKQITSLNATRTADGQNFDADSKAKIADFKKQVSESLKAANYPAKADPAQMNTIMVLVILVILVIYVT
MVYGPIAAMLVEMFPTRIRYSSMSLPYHIGNGWFGGLLPTISFALVAQNGNIYHGLWYPIWIAAITFVIGVLFVRETKDV
DIYRND

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=325, Percent_Identity=38.7692307692308, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1788292, Length=325, Percent_Identity=36.3076923076923, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1788942, Length=327, Percent_Identity=33.3333333333333, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1789941, Length=342, Percent_Identity=34.5029239766082, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87082231, Length=222, Percent_Identity=26.1261261261261, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87082404, Length=316, Percent_Identity=24.6835443037975, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 61578; Mature: 61447

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVQSAPNAPVGRVQPAPMTREEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFSGL
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DPTAGFIFALLAFAAGFLVRPFGALVFGRLGDMIGRKYTFLVTIVIMGTSTFIVGLLPSY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
GTIGVAAPVILIALRMLQGLALGGEYGGAATYVAEHAPHGRRGAYTSWIQTTATLGLFLS
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
LVVILAVRELTGKAFDDWGWRIPFLVSILLLATSVYIRLSMSESPAFQKMKAEGKTSKAP
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHCCCCCCCC
LTEAFGQWRNLKIVILALLGLTAGQAVVWYTGQFYALFFLTQVLKVDAFTANVLIAVALA
HHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGTPFFVFFGSLSDRIGRKKIIMAGCLLAVLTYFPLFKALTHYANPALERAQQTAQITIT
HCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEEEEE
VDPKECSFQGSPIAREVDFRSSCDIAKRTLAQSSASYETVEGPAGTVASVSIAGKQITSL
ECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHCC
NATRTADGQNFDADSKAKIADFKKQVSESLKAANYPAKADPAQMNTIMVLVILVILVIYV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TMVYGPIAAMLVEMFPTRIRYSSMSLPYHIGNGWFGGLLPTISFALVAQNGNIYHGLWYP
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHEEEEECCCCEEEHHHHH
IWIAAITFVIGVLFVRETKDVDIYRND
HHHHHHHHHHHHHHHHCCCCCCCEECC
>Mature Secondary Structure 
ATVQSAPNAPVGRVQPAPMTREEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFSGL
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DPTAGFIFALLAFAAGFLVRPFGALVFGRLGDMIGRKYTFLVTIVIMGTSTFIVGLLPSY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
GTIGVAAPVILIALRMLQGLALGGEYGGAATYVAEHAPHGRRGAYTSWIQTTATLGLFLS
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
LVVILAVRELTGKAFDDWGWRIPFLVSILLLATSVYIRLSMSESPAFQKMKAEGKTSKAP
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHCCCCCCCC
LTEAFGQWRNLKIVILALLGLTAGQAVVWYTGQFYALFFLTQVLKVDAFTANVLIAVALA
HHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGTPFFVFFGSLSDRIGRKKIIMAGCLLAVLTYFPLFKALTHYANPALERAQQTAQITIT
HCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEEEEE
VDPKECSFQGSPIAREVDFRSSCDIAKRTLAQSSASYETVEGPAGTVASVSIAGKQITSL
ECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHCC
NATRTADGQNFDADSKAKIADFKKQVSESLKAANYPAKADPAQMNTIMVLVILVILVIYV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TMVYGPIAAMLVEMFPTRIRYSSMSLPYHIGNGWFGGLLPTISFALVAQNGNIYHGLWYP
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHEEEEECCCCEEEHHHHH
IWIAAITFVIGVLFVRETKDVDIYRND
HHHHHHHHHHHHHHHHCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]