Click here to switch to the map view.

The map label for this gene is ksgA [H]

Identifier: 187927497

GI number: 187927497

Start: 420190

End: 421035

Strand: Direct

Name: ksgA [H]

Synonym: Rpic_0394

Alternate gene names: 187927497

Gene position: 420190-421035 (Clockwise)

Preceding gene: 187927496

Following gene: 187927522

Centisome position: 10.66

GC content: 64.89

Gene sequence:

>846_bases
ATGGCACGATCCACGACCGGCGTTCACCAAGGGCACCAGGCCCGCAAGCGCTTCGGCCAGAACTTCCTGGTCGATGACGG
CGTGATCCACGCCATCGTTGCCGCCATCGATCCGAAGCCCGACGATGTACTTGTCGAGATCGGCCCCGGCCTGGGCGCGC
TCACGGTGCCGCTGATGGAGCGCGTGCGCACGCTGCAGGTGGTCGAGCTGGATCGAGACCTTGTCGCGCGCTTGCAGCAG
CGATTCGGTGATCGGCTGATCGTGCATGCGGGCGATGCACTGGCGTTCGATTTCGGTTCGCTGCAGGAGGCGGGCCGGCC
GCTGCGCATCGTCGGCAACCTGCCGTACAACATCTCGAGCCCGCTGCTGTTTCATCTGGCCACGTTCGCCGATCGTGTGC
GCGATCAGCATTTCATGCTGCAGAAAGAAGTGGTCGACCGCATGGTCGCCGCGCCGGGTAGCAAGGCGTTCAGCCGGCTG
TCGGTGATGCTGCAGGTGCGGTACTACATGGAGCTGGTGCTGGATGTGCCGCCGGGCTCGTTCAACCCGCCGCCCAAAGT
GGATTCGGCGGTGGTGCGGATGATTCCGTGGCCGGCCGACAAATCGCCGTATGCGCCGGTCGACCTGCGCGCGCTTGGCA
CGGTGGTGACGATGGCGTTTTCGCAGCGCCGCAAGGTGTTGCGCAACACGCTGGGCGCGCTGCGCGATGTGATCGATTTC
GAAGCGCTTGGCTTTGACCTGGGCCGGCGCGCAGAAGAAGTGTCGGTGGAAGACTTCGTGGCAGTGGCGAACGCACTGCC
GGCCAATCGCATTGCCGGCAGCGCCTCCGACCTTAGCGAAGATTGA

Upstream 100 bases:

>100_bases
AGTTCGGCAGCATGATCGAGGCGATCGATACTGCCATCGCCATGGCGCGGCACACCGCGCGCTCCTGAGTTTCCTTCACC
TCTTCGATTTCGAGTTCGCA

Downstream 100 bases:

>100_bases
CGAGCAGGATGCCCGACAGCACGAGCGCCGCCGCCAGAGCAAACCGCAGTCCCACCGGCTCGTGCAACAGCAGCACGCCA
AACGCCACGCCAAACAGCGG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MARSTTGVHQGHQARKRFGQNFLVDDGVIHAIVAAIDPKPDDVLVEIGPGLGALTVPLMERVRTLQVVELDRDLVARLQQ
RFGDRLIVHAGDALAFDFGSLQEAGRPLRIVGNLPYNISSPLLFHLATFADRVRDQHFMLQKEVVDRMVAAPGSKAFSRL
SVMLQVRYYMELVLDVPPGSFNPPPKVDSAVVRMIPWPADKSPYAPVDLRALGTVVTMAFSQRRKVLRNTLGALRDVIDF
EALGFDLGRRAEEVSVEDFVAVANALPANRIAGSASDLSED

Sequences:

>Translated_281_residues
MARSTTGVHQGHQARKRFGQNFLVDDGVIHAIVAAIDPKPDDVLVEIGPGLGALTVPLMERVRTLQVVELDRDLVARLQQ
RFGDRLIVHAGDALAFDFGSLQEAGRPLRIVGNLPYNISSPLLFHLATFADRVRDQHFMLQKEVVDRMVAAPGSKAFSRL
SVMLQVRYYMELVLDVPPGSFNPPPKVDSAVVRMIPWPADKSPYAPVDLRALGTVVTMAFSQRRKVLRNTLGALRDVIDF
EALGFDLGRRAEEVSVEDFVAVANALPANRIAGSASDLSED
>Mature_280_residues
ARSTTGVHQGHQARKRFGQNFLVDDGVIHAIVAAIDPKPDDVLVEIGPGLGALTVPLMERVRTLQVVELDRDLVARLQQR
FGDRLIVHAGDALAFDFGSLQEAGRPLRIVGNLPYNISSPLLFHLATFADRVRDQHFMLQKEVVDRMVAAPGSKAFSRLS
VMLQVRYYMELVLDVPPGSFNPPPKVDSAVVRMIPWPADKSPYAPVDLRALGTVVTMAFSQRRKVLRNTLGALRDVIDFE
ALGFDLGRRAEEVSVEDFVAVANALPANRIAGSASDLSED

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=214, Percent_Identity=35.0467289719626, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI156415992, Length=247, Percent_Identity=32.7935222672065, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1786236, Length=267, Percent_Identity=54.6816479400749, Blast_Score=280, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI25146882, Length=231, Percent_Identity=35.9307359307359, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI25141369, Length=248, Percent_Identity=28.6290322580645, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6324989, Length=210, Percent_Identity=30, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21358017, Length=255, Percent_Identity=32.5490196078431, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21357273, Length=286, Percent_Identity=29.7202797202797, Blast_Score=98, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 30858; Mature: 30727

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSTTGVHQGHQARKRFGQNFLVDDGVIHAIVAAIDPKPDDVLVEIGPGLGALTVPLME
CCCCCCCHHCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHH
RVRTLQVVELDRDLVARLQQRFGDRLIVHAGDALAFDFGSLQEAGRPLRIVGNLPYNISS
HHHHHHHHHHCHHHHHHHHHHHCCEEEEEECCCCEECCHHHHHCCCCEEEEECCCCCCCC
PLLFHLATFADRVRDQHFMLQKEVVDRMVAAPGSKAFSRLSVMLQVRYYMELVLDVPPGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
FNPPPKVDSAVVRMIPWPADKSPYAPVDLRALGTVVTMAFSQRRKVLRNTLGALRDVIDF
CCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALGFDLGRRAEEVSVEDFVAVANALPANRIAGSASDLSED
HHHHHHHHCCHHHCCHHHHHHHHHCCCHHHHCCCCCCCCCC
>Mature Secondary Structure 
ARSTTGVHQGHQARKRFGQNFLVDDGVIHAIVAAIDPKPDDVLVEIGPGLGALTVPLME
CCCCCCHHCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHH
RVRTLQVVELDRDLVARLQQRFGDRLIVHAGDALAFDFGSLQEAGRPLRIVGNLPYNISS
HHHHHHHHHHCHHHHHHHHHHHCCEEEEEECCCCEECCHHHHHCCCCEEEEECCCCCCCC
PLLFHLATFADRVRDQHFMLQKEVVDRMVAAPGSKAFSRLSVMLQVRYYMELVLDVPPGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
FNPPPKVDSAVVRMIPWPADKSPYAPVDLRALGTVVTMAFSQRRKVLRNTLGALRDVIDF
CCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALGFDLGRRAEEVSVEDFVAVANALPANRIAGSASDLSED
HHHHHHHHCCHHHCCHHHHHHHHHCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]