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The map label for this gene is tyrS [H]

Identifier: 187927475

GI number: 187927475

Start: 395884

End: 397149

Strand: Direct

Name: tyrS [H]

Synonym: Rpic_0372

Alternate gene names: 187927475

Gene position: 395884-397149 (Clockwise)

Preceding gene: 187927473

Following gene: 187927476

Centisome position: 10.04

GC content: 64.14

Gene sequence:

>1266_bases
ATGACTGCATCTGCCACTCCCGCCGCTTCCGACAACGCCACGCCGGGCACACTCAAGTATCCGGTCACCCCGGAGGTGGC
GCACGCGATGGAAGTCTCGCTGCGCGGCTGCGACGAACTGCTGATCGCCGCCGAGTGGGAGCAGAAGCTGGCCAAGAGCG
CTGCCACCGGCGTACCCCTGCGCATCAAGCTGGGCCTCGATCCCACGGCCCCTGACATCCACATCGGCCACACGGTGGTG
CTCAACAAGATGCGCCAGTTGCAGGACCTAGGGCATCAGGTGATCTTCCTGATCGGCGACTTCACGTCGACCATTGGTGA
CCCGTCCGGCCGCAACGCCACGCGCCCGCCGCTGACGCGCGAGCAGATCGAAGCCAACGCGCAGACGTACTACCGCCAGG
CCAGCATGGTGCTGGACCCGAGCAAGACCGAAATTCGCTACAACAGCGAGTGGTGCGACCCGCTGGGCGCGCGCGGCATC
ATCCAACTCGCCGCCAAGTACACCGTCGCCCGCATGATGGAGCGCGATGACTTCACCAAGCGCTACAAGGCCGGCGTGCC
GATCTCGGTGCACGAATTCCTCTACCCGCTGATGCAGGGCTATGACTCGGTCGCGCTCAAATCGGATCTGGAACTGGGCG
GCACCGATCAGAAGTTCAACCTGCTGGTCGGCCGCGAACTGCAGAAGGAATACGGCCAGGAGCCGCAGTGCATCCTGACG
ATGCCGCTGCTGGTGGGCCTGGACGGCGTCGAGAAGATGTCCAAGTCCAAGGGCAACTACGTCGGTATCAGCGAGGCGCC
CAACGAGATGTTCGGCAAGCTGATGAGCATCTCCGATGACCTGATGTGGACGTACTACACGCTGCTGTCGTTCCGCCCGC
TGGCCGAGATCGACCTGATGAAGCAAGAGATCGCGCTGGGCCGCAACCCGCGCGATTGCAAGGTGATGCTGGCGCAGGAG
ATCGTTGCCCGCTTCCACAGCCAGGCCGATGCCGAGCGCGCACTGGAAGATTTCAACCACCGTGCCCGCGGCGGTGTGCC
GGACGACATTCCGCAGATCGAGCTGTCGGGCGCACCACTGGGTATTGCACAACTGCTCAAGCAGGCTGGGCTCTGCCCGT
CCACGTCGGACGCCAACCGCAACATCGAGCAGGGCGGCGTGAAGATCGACGGCACCGTCATCAGCGACAAGGGCCTGAAG
GTCGACGCCGGCACCTTCGTGATGCAGGTCGGCAAGCGTCGCTTTGCGCGCGTGGTGCTGAAGTGA

Upstream 100 bases:

>100_bases
CGCCTGAATCGATGTCGGCTATCGTGCACGCACCGTTTCCCTGATTGTTGATCCGTTCGCGCCGTTTTCCACGTGCGGCG
CGGTGACCTTTCCTTTTGCC

Downstream 100 bases:

>100_bases
TTGGGCTGATCCAGCGTGTGTCCGAGGCGGCGGTCCGTGTGGACGGCCGCGTCGTCGGCGAGATCGGCCCGGGTTTGCTC
GCACTGGTCTGTGCAGAACG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTASATPAASDNATPGTLKYPVTPEVAHAMEVSLRGCDELLIAAEWEQKLAKSAATGVPLRIKLGLDPTAPDIHIGHTVV
LNKMRQLQDLGHQVIFLIGDFTSTIGDPSGRNATRPPLTREQIEANAQTYYRQASMVLDPSKTEIRYNSEWCDPLGARGI
IQLAAKYTVARMMERDDFTKRYKAGVPISVHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRELQKEYGQEPQCILT
MPLLVGLDGVEKMSKSKGNYVGISEAPNEMFGKLMSISDDLMWTYYTLLSFRPLAEIDLMKQEIALGRNPRDCKVMLAQE
IVARFHSQADAERALEDFNHRARGGVPDDIPQIELSGAPLGIAQLLKQAGLCPSTSDANRNIEQGGVKIDGTVISDKGLK
VDAGTFVMQVGKRRFARVVLK

Sequences:

>Translated_421_residues
MTASATPAASDNATPGTLKYPVTPEVAHAMEVSLRGCDELLIAAEWEQKLAKSAATGVPLRIKLGLDPTAPDIHIGHTVV
LNKMRQLQDLGHQVIFLIGDFTSTIGDPSGRNATRPPLTREQIEANAQTYYRQASMVLDPSKTEIRYNSEWCDPLGARGI
IQLAAKYTVARMMERDDFTKRYKAGVPISVHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRELQKEYGQEPQCILT
MPLLVGLDGVEKMSKSKGNYVGISEAPNEMFGKLMSISDDLMWTYYTLLSFRPLAEIDLMKQEIALGRNPRDCKVMLAQE
IVARFHSQADAERALEDFNHRARGGVPDDIPQIELSGAPLGIAQLLKQAGLCPSTSDANRNIEQGGVKIDGTVISDKGLK
VDAGTFVMQVGKRRFARVVLK
>Mature_420_residues
TASATPAASDNATPGTLKYPVTPEVAHAMEVSLRGCDELLIAAEWEQKLAKSAATGVPLRIKLGLDPTAPDIHIGHTVVL
NKMRQLQDLGHQVIFLIGDFTSTIGDPSGRNATRPPLTREQIEANAQTYYRQASMVLDPSKTEIRYNSEWCDPLGARGII
QLAAKYTVARMMERDDFTKRYKAGVPISVHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRELQKEYGQEPQCILTM
PLLVGLDGVEKMSKSKGNYVGISEAPNEMFGKLMSISDDLMWTYYTLLSFRPLAEIDLMKQEIALGRNPRDCKVMLAQEI
VARFHSQADAERALEDFNHRARGGVPDDIPQIELSGAPLGIAQLLKQAGLCPSTSDANRNIEQGGVKIDGTVISDKGLKV
DAGTFVMQVGKRRFARVVLK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=417, Percent_Identity=26.6187050359712, Blast_Score=106, Evalue=5e-23,
Organism=Escherichia coli, GI1787925, Length=380, Percent_Identity=27.8947368421053, Blast_Score=121, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI25145802, Length=261, Percent_Identity=27.2030651340996, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6325160, Length=403, Percent_Identity=23.0769230769231, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20130383, Length=401, Percent_Identity=25.6857855361596, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR000253
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46293; Mature: 46162

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50006 FHA_DOMAIN ; PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASATPAASDNATPGTLKYPVTPEVAHAMEVSLRGCDELLIAAEWEQKLAKSAATGVPL
CCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCE
RIKLGLDPTAPDIHIGHTVVLNKMRQLQDLGHQVIFLIGDFTSTIGDPSGRNATRPPLTR
EEEECCCCCCCCEECCHHHHHHHHHHHHHCCCEEEEEEECHHHHCCCCCCCCCCCCCCCH
EQIEANAQTYYRQASMVLDPSKTEIRYNSEWCDPLGARGIIQLAAKYTVARMMERDDFTK
HHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RYKAGVPISVHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRELQKEYGQEPQCILT
HHHCCCCEEHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEE
MPLLVGLDGVEKMSKSKGNYVGISEAPNEMFGKLMSISDDLMWTYYTLLSFRPLAEIDLM
CHHHHCCCHHHHHHHCCCCEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHH
KQEIALGRNPRDCKVMLAQEIVARFHSQADAERALEDFNHRARGGVPDDIPQIELSGAPL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCH
GIAQLLKQAGLCPSTSDANRNIEQGGVKIDGTVISDKGLKVDAGTFVMQVGKRRFARVVL
HHHHHHHHCCCCCCCCCCCCCHHHCCEEEEEEEECCCCCEECHHHHHHHHHHHHHHHHHC
K
C
>Mature Secondary Structure 
TASATPAASDNATPGTLKYPVTPEVAHAMEVSLRGCDELLIAAEWEQKLAKSAATGVPL
CCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCE
RIKLGLDPTAPDIHIGHTVVLNKMRQLQDLGHQVIFLIGDFTSTIGDPSGRNATRPPLTR
EEEECCCCCCCCEECCHHHHHHHHHHHHHCCCEEEEEEECHHHHCCCCCCCCCCCCCCCH
EQIEANAQTYYRQASMVLDPSKTEIRYNSEWCDPLGARGIIQLAAKYTVARMMERDDFTK
HHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RYKAGVPISVHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRELQKEYGQEPQCILT
HHHCCCCEEHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEE
MPLLVGLDGVEKMSKSKGNYVGISEAPNEMFGKLMSISDDLMWTYYTLLSFRPLAEIDLM
CHHHHCCCHHHHHHHCCCCEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHH
KQEIALGRNPRDCKVMLAQEIVARFHSQADAERALEDFNHRARGGVPDDIPQIELSGAPL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCH
GIAQLLKQAGLCPSTSDANRNIEQGGVKIDGTVISDKGLKVDAGTFVMQVGKRRFARVVL
HHHHHHHHCCCCCCCCCCCCCHHHCCEEEEEEEECCCCCEECHHHHHHHHHHHHHHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]