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The map label for this gene is gdhA [H]

Identifier: 187927459

GI number: 187927459

Start: 380869

End: 382170

Strand: Direct

Name: gdhA [H]

Synonym: Rpic_0356

Alternate gene names: 187927459

Gene position: 380869-382170 (Clockwise)

Preceding gene: 187927458

Following gene: 187927460

Centisome position: 9.66

GC content: 63.44

Gene sequence:

>1302_bases
ATGTCCAATGTCGCATCTCCGTCGACACTCCAGCACGCCATCCCGTCCTATCTGCCGGCCGACAATCTCGGCCCGTGGGG
CATCTATCTGCAGCAGGTCGATCGGGTTACCCCCTACCTCGGCTCGCTTGCACGTTGGGTTGAAACGCTCAAGCGCCCGA
AGCGCGCGCTGATCGTCGACGTGCCGATCCAGATGGACGACGGCCGCATCGCCCACTTCGAGGGCTATCGCGTGCAGCAC
AACACGTCGCGCGGCCCGGGCAAGGGCGGCGTGCGTTTCCACCAGGACGTGACGCTGTCGGAAGTGATGGCGTTGTCTGC
CTGGATGTCGGTGAAGAATGCGGCCGTGAATGTGCCGTACGGTGGTGCCAAGGGTGGTATCCGCGTCGACCCGCGCAAGC
TGTCTTCCGGCGAACTTGAACGCCTGACGCGCCGCTACACGAGCGAGATCGGCATCATCATCGGCCCGAACAAGGACATT
CCGGCCCCGGACGTGAACACCAACGCGCAGATCATGGCGTGGATGATGGACACGTACTCCATGAACGAAGGCTCCACCGC
CACTGGCGTGGTGACCGGCAAGCCGATCGCACTGGGCGGCAGCCTGGGCCGTCGCGAAGCCACTGGCCGCGGCGTGTTCG
TGGTCGGCAGCGAAGCCGCACGCAACCTCGGGATCGACGTCAAGGGCGCGCGCGTGGTGGTGCAGGGTTTCGGCAACGTG
GGCAGCGTGGCAGCCAAGCTGTTCCACGACGCTGGTGCCAAGGTCATTGCCGTGCAAGACCACAAGGGCATTGTGTTCAA
CGGCAACGGCCTGGATGTCGACGCGCTCATCAAGCACGTTGACCACAATGGCAGCGTGGCCGGCTTTGCTGCAGAGGCCG
TCTCGCAAGACGACTTCTGGGCGCTGGACTGCGAATTCCTGATCCCGGCCGCGCTGGAAGGCCAGATCACTGCCAAGAAC
GCGCCTCATATCAAGGCAAAGATTGTCGTGGAAGGTGCAAACGGCCCGACGACGCCCGAGGCGGACGACATCCTGCGCGA
CCGGGGCATCCTGGTCTGCCCGGACGTGATCGCCAACGCTGGCGGCGTAACGGTCAGCTATTTCGAATGGGTGCAGGATT
TCTCGAGCTTCTTCTGGACCGAAGACGAGATCAACCAGCGTCTGGTACGGATCATGCAAGACGCCTTCCGTGGCGTCTGG
CAAGTGGCGCAGGACAACAAGGTTACTCTGCGTACGGCGGCGTTCATCATCGCCTGTACGCGGATTCTGCAGGCCCGCCA
AGAGCGCGGTCTGTACCCCTGA

Upstream 100 bases:

>100_bases
GTAAAAATGGCATTGGATTTCGTTCCGGGCGGTCCCTAAGCTGGCGGGCATTTTGTGGGACTTGAAGGGCCTTACCGAGG
CCCAAAAAAGGAAGACAGCC

Downstream 100 bases:

>100_bases
TCGGCAACGCGCTGGTCAATACCGGCGCGGTGCTCCAACAGGGTGCTGCACCGGTCTATCGCGTTGCTGCCGATCAGGCG
GCCATGCGCGACGGTTGAAG

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSNVASPSTLQHAIPSYLPADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRALIVDVPIQMDDGRIAHFEGYRVQH
NTSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRKLSSGELERLTRRYTSEIGIIIGPNKDI
PAPDVNTNAQIMAWMMDTYSMNEGSTATGVVTGKPIALGGSLGRREATGRGVFVVGSEAARNLGIDVKGARVVVQGFGNV
GSVAAKLFHDAGAKVIAVQDHKGIVFNGNGLDVDALIKHVDHNGSVAGFAAEAVSQDDFWALDCEFLIPAALEGQITAKN
APHIKAKIVVEGANGPTTPEADDILRDRGILVCPDVIANAGGVTVSYFEWVQDFSSFFWTEDEINQRLVRIMQDAFRGVW
QVAQDNKVTLRTAAFIIACTRILQARQERGLYP

Sequences:

>Translated_433_residues
MSNVASPSTLQHAIPSYLPADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRALIVDVPIQMDDGRIAHFEGYRVQH
NTSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRKLSSGELERLTRRYTSEIGIIIGPNKDI
PAPDVNTNAQIMAWMMDTYSMNEGSTATGVVTGKPIALGGSLGRREATGRGVFVVGSEAARNLGIDVKGARVVVQGFGNV
GSVAAKLFHDAGAKVIAVQDHKGIVFNGNGLDVDALIKHVDHNGSVAGFAAEAVSQDDFWALDCEFLIPAALEGQITAKN
APHIKAKIVVEGANGPTTPEADDILRDRGILVCPDVIANAGGVTVSYFEWVQDFSSFFWTEDEINQRLVRIMQDAFRGVW
QVAQDNKVTLRTAAFIIACTRILQARQERGLYP
>Mature_432_residues
SNVASPSTLQHAIPSYLPADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRALIVDVPIQMDDGRIAHFEGYRVQHN
TSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRKLSSGELERLTRRYTSEIGIIIGPNKDIP
APDVNTNAQIMAWMMDTYSMNEGSTATGVVTGKPIALGGSLGRREATGRGVFVVGSEAARNLGIDVKGARVVVQGFGNVG
SVAAKLFHDAGAKVIAVQDHKGIVFNGNGLDVDALIKHVDHNGSVAGFAAEAVSQDDFWALDCEFLIPAALEGQITAKNA
PHIKAKIVVEGANGPTTPEADDILRDRGILVCPDVIANAGGVTVSYFEWVQDFSSFFWTEDEINQRLVRIMQDAFRGVWQ
VAQDNKVTLRTAAFIIACTRILQARQERGLYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=369, Percent_Identity=43.0894308943089, Blast_Score=292, Evalue=5e-79,
Organism=Homo sapiens, GI4885281, Length=369, Percent_Identity=42.5474254742547, Blast_Score=285, Evalue=5e-77,
Organism=Escherichia coli, GI1788059, Length=362, Percent_Identity=33.7016574585635, Blast_Score=180, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17544676, Length=370, Percent_Identity=40.2702702702703, Blast_Score=266, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6319256, Length=383, Percent_Identity=30.2872062663185, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6324951, Length=374, Percent_Identity=29.144385026738, Blast_Score=140, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24649283, Length=463, Percent_Identity=36.9330453563715, Blast_Score=281, Evalue=4e-76,
Organism=Drosophila melanogaster, GI45553475, Length=358, Percent_Identity=42.1787709497207, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI45549226, Length=361, Percent_Identity=41.8282548476454, Blast_Score=271, Evalue=5e-73,
Organism=Drosophila melanogaster, GI24649460, Length=307, Percent_Identity=41.6938110749186, Blast_Score=224, Evalue=7e-59,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46784; Mature: 46653

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVASPSTLQHAIPSYLPADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRALIVD
CCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
VPIQMDDGRIAHFEGYRVQHNTSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNVPY
ECEEECCCEEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCEEECCC
GGAKGGIRVDPRKLSSGELERLTRRYTSEIGIIIGPNKDIPAPDVNTNAQIMAWMMDTYS
CCCCCCEEECCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCEEEEEEEHHHC
MNEGSTATGVVTGKPIALGGSLGRREATGRGVFVVGSEAARNLGIDVKGARVVVQGFGNV
CCCCCCEEEEEECCCEEECCCCCCCCCCCCEEEEECCHHHHHCCCCCCCCEEEEECCCCH
GSVAAKLFHDAGAKVIAVQDHKGIVFNGNGLDVDALIKHVDHNGSVAGFAAEAVSQDDFW
HHHHHHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHCCCCCEEEHHHHHCCCCCCE
ALDCEFLIPAALEGQITAKNAPHIKAKIVVEGANGPTTPEADDILRDRGILVCPDVIANA
EEEEHEECCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCEEECHHHCCC
GGVTVSYFEWVQDFSSFFWTEDEINQRLVRIMQDAFRGVWQVAQDNKVTLRTAAFIIACT
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHH
RILQARQERGLYP
HHHHHHHHCCCCC
>Mature Secondary Structure 
SNVASPSTLQHAIPSYLPADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRALIVD
CCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
VPIQMDDGRIAHFEGYRVQHNTSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNVPY
ECEEECCCEEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCEEECCC
GGAKGGIRVDPRKLSSGELERLTRRYTSEIGIIIGPNKDIPAPDVNTNAQIMAWMMDTYS
CCCCCCEEECCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCEEEEEEEHHHC
MNEGSTATGVVTGKPIALGGSLGRREATGRGVFVVGSEAARNLGIDVKGARVVVQGFGNV
CCCCCCEEEEEECCCEEECCCCCCCCCCCCEEEEECCHHHHHCCCCCCCCEEEEECCCCH
GSVAAKLFHDAGAKVIAVQDHKGIVFNGNGLDVDALIKHVDHNGSVAGFAAEAVSQDDFW
HHHHHHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHCCCCCEEEHHHHHCCCCCCE
ALDCEFLIPAALEGQITAKNAPHIKAKIVVEGANGPTTPEADDILRDRGILVCPDVIANA
EEEEHEECCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCEEECHHHCCC
GGVTVSYFEWVQDFSSFFWTEDEINQRLVRIMQDAFRGVWQVAQDNKVTLRTAAFIIACT
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHH
RILQARQERGLYP
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]