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The map label for this gene is mlhB [H]

Identifier: 187927418

GI number: 187927418

Start: 331364

End: 332335

Strand: Direct

Name: mlhB [H]

Synonym: Rpic_0315

Alternate gene names: 187927418

Gene position: 331364-332335 (Clockwise)

Preceding gene: 187927415

Following gene: 187927419

Centisome position: 8.4

GC content: 66.26

Gene sequence:

>972_bases
ATGAGCTGGCAGAACTGGGTCGTCTCTAGGATGGTGCGCAACCAGATCAAGAAGCCGACCGAGCGGGAGCCCTTCGACCC
GATCGCCACACGCCTGCATGCCGAGAAGCGCAAGATGCCTTCGCCGGTGGAGCTGCCGCCGGATTGGCGTATCGTCCCGG
CCGATGGTGTCAACGGCGATGGCCTCACCGGCGAATGGATTGAACCCGCCGATCCGGACCTGCATGACGACGCGCCGGTC
GTGCTTTATCTGCATGGCGGCGGCTATTTCTTCTGCAGCCCGCGCACACATCGCCCGATCACCATTGGCCTCGCCACGCA
TGCCCGGGCGCGGGTGTGCGTGCCGGACTACCGGTTGGCACCCGAGCATCCGTTTCCTGCGCCGGTGGAAGATGCGCTCT
TCACGTATCGCACGCTGGTGACGCAGGGCGTGTCGCCCTCGCGCATTGTGGTGGCCGGCGATTCCGCGGGCGGCGGGCTG
GCGTTGGCGCTGCTGGTGGCCTTGCGTGATGCGGCAGACCCGCTGCCGGCGGGCGCGGTGCTGTATTCGCCGTGGACGGA
TCTTGCCGCCACCGGCCAGAGCCTGATCGACAACGATGAATCCGACGTGATGTTTCGCGGCGCGTGGATGGCGCATGGTG
CGGCGTTGTACCTGGGCGATTCGGGCGCACCGGCCGACCATCCGCTCGCCTCGCCGCTGTATGCCGATTTCACGGGCCTG
CCCACACTGCACTGCTATGTCAGCACCAGCGAAGTGCTGCGCGACGATACCCTCCGAATGGCCGAGCGGGCGCGGGCGGC
CGGGGTATCCGTATCGGTAGAAATGGGGCGGCGGCTTCCTCACGTGTGGCCGATCTTCTATCCTTTCCTTCCAGAGGCGC
GCGTAGCGCTCAAACGATCGGGCGAGCAGATCAGGCGACTGGTTGCCGCCCAGCACAAGAACCGAGAACCGGTGGAACTG
ACACCGGCCTGA

Upstream 100 bases:

>100_bases
GTTGCCCGATGGGGACACTCGCCGTTCAAACAGGGTGCGAACCGATTGTCTGGGTAGCGCTTCCTCGCTAGGCTGGGACT
TTGCAGCGGAGGGTTGCGCG

Downstream 100 bases:

>100_bases
CCCAGCCGGCGGCGCCCCTGCGTAGCCCGGTCGGGATGCGCAAACGGGGAGCCCAGCATGCGTCGGGTGGTGGTATTGGG
TGTGGTGCTGGCCGTGGTGG

Product: alpha/beta hydrolase fold-3 domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSWQNWVVSRMVRNQIKKPTEREPFDPIATRLHAEKRKMPSPVELPPDWRIVPADGVNGDGLTGEWIEPADPDLHDDAPV
VLYLHGGGYFFCSPRTHRPITIGLATHARARVCVPDYRLAPEHPFPAPVEDALFTYRTLVTQGVSPSRIVVAGDSAGGGL
ALALLVALRDAADPLPAGAVLYSPWTDLAATGQSLIDNDESDVMFRGAWMAHGAALYLGDSGAPADHPLASPLYADFTGL
PTLHCYVSTSEVLRDDTLRMAERARAAGVSVSVEMGRRLPHVWPIFYPFLPEARVALKRSGEQIRRLVAAQHKNREPVEL
TPA

Sequences:

>Translated_323_residues
MSWQNWVVSRMVRNQIKKPTEREPFDPIATRLHAEKRKMPSPVELPPDWRIVPADGVNGDGLTGEWIEPADPDLHDDAPV
VLYLHGGGYFFCSPRTHRPITIGLATHARARVCVPDYRLAPEHPFPAPVEDALFTYRTLVTQGVSPSRIVVAGDSAGGGL
ALALLVALRDAADPLPAGAVLYSPWTDLAATGQSLIDNDESDVMFRGAWMAHGAALYLGDSGAPADHPLASPLYADFTGL
PTLHCYVSTSEVLRDDTLRMAERARAAGVSVSVEMGRRLPHVWPIFYPFLPEARVALKRSGEQIRRLVAAQHKNREPVEL
TPA
>Mature_322_residues
SWQNWVVSRMVRNQIKKPTEREPFDPIATRLHAEKRKMPSPVELPPDWRIVPADGVNGDGLTGEWIEPADPDLHDDAPVV
LYLHGGGYFFCSPRTHRPITIGLATHARARVCVPDYRLAPEHPFPAPVEDALFTYRTLVTQGVSPSRIVVAGDSAGGGLA
LALLVALRDAADPLPAGAVLYSPWTDLAATGQSLIDNDESDVMFRGAWMAHGAALYLGDSGAPADHPLASPLYADFTGLP
TLHCYVSTSEVLRDDTLRMAERARAAGVSVSVEMGRRLPHVWPIFYPFLPEARVALKRSGEQIRRLVAAQHKNREPVELT
PA

Specific function: Lactone hydrolase that is required for the degradation of monocyclic monoterpenes such as menthol and carvone. Catalyzes the ring opening of lactones formed during degradation of monocyclic monoterpenes. Can hydrolyze epsilon-caprolactone, the lactone der

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=101, Percent_Identity=41.5841584158416, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI157041239, Length=99, Percent_Identity=38.3838383838384, Blast_Score=66, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI115533410, Length=112, Percent_Identity=35.7142857142857, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115533412, Length=112, Percent_Identity=35.7142857142857, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.83 [H]

Molecular weight: Translated: 35338; Mature: 35206

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWQNWVVSRMVRNQIKKPTEREPFDPIATRLHAEKRKMPSPVELPPDWRIVPADGVNGD
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCC
GLTGEWIEPADPDLHDDAPVVLYLHGGGYFFCSPRTHRPITIGLATHARARVCVPDYRLA
CCCCCCCCCCCCCCCCCCCEEEEEECCEEEEECCCCCCCEEEEEECCCCEEEECCCCCCC
PEHPFPAPVEDALFTYRTLVTQGVSPSRIVVAGDSAGGGLALALLVALRDAADPLPAGAV
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEE
LYSPWTDLAATGQSLIDNDESDVMFRGAWMAHGAALYLGDSGAPADHPLASPLYADFTGL
EECCCHHHHHCCHHHHCCCCCCEEEEEEHHHCCEEEEECCCCCCCCCCCCCCHHHCCCCC
PTLHCYVSTSEVLRDDTLRMAERARAAGVSVSVEMGRRLPHVWPIFYPFLPEARVALKRS
CEEEEEECHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCCCCHHHHHHCCCCHHHHHHHHC
GEQIRRLVAAQHKNREPVELTPA
HHHHHHHHHHHHCCCCCEEECCC
>Mature Secondary Structure 
SWQNWVVSRMVRNQIKKPTEREPFDPIATRLHAEKRKMPSPVELPPDWRIVPADGVNGD
CHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCC
GLTGEWIEPADPDLHDDAPVVLYLHGGGYFFCSPRTHRPITIGLATHARARVCVPDYRLA
CCCCCCCCCCCCCCCCCCCEEEEEECCEEEEECCCCCCCEEEEEECCCCEEEECCCCCCC
PEHPFPAPVEDALFTYRTLVTQGVSPSRIVVAGDSAGGGLALALLVALRDAADPLPAGAV
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEE
LYSPWTDLAATGQSLIDNDESDVMFRGAWMAHGAALYLGDSGAPADHPLASPLYADFTGL
EECCCHHHHHCCHHHHCCCCCCEEEEEEHHHCCEEEEECCCCCCCCCCCCCCHHHCCCCC
PTLHCYVSTSEVLRDDTLRMAERARAAGVSVSVEMGRRLPHVWPIFYPFLPEARVALKRS
CEEEEEECHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCCCCHHHHHHCCCCHHHHHHHHC
GEQIRRLVAAQHKNREPVELTPA
HHHHHHHHHHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA