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The map label for this gene is aceK

Identifier: 187927236

GI number: 187927236

Start: 147847

End: 149682

Strand: Reverse

Name: aceK

Synonym: Rpic_0128

Alternate gene names: 187927236

Gene position: 149682-147847 (Counterclockwise)

Preceding gene: 187927237

Following gene: 187927235

Centisome position: 3.8

GC content: 60.51

Gene sequence:

>1836_bases
ATGTCCCATTTCCCCAAGCTCCTCTCGTCGCAGATCGCCTTCGACGTTGCCCGCACGATGCTCGACGGCTTCGACAAGCA
CTATCGCTTGTTCCGCGAGGTCAGCGTACAGGCCAAGACGGCGTTCGAGACCGGAGACTGGGCCGGCCTGCAGCAGTTGC
AGCGCGACCGCATTGCTTATTACGACGAGCGCGTACGTGAAGCCTCGGTCATCCTCGAAGACGAATACGACGCCGAGAAC
ATCGACGACGAGATCTGGCGCCAGATCAAGCTGCACTTCATCGGCCTGCTGACCAACCATCATCAGCCCGAGTGCGCCGA
GACCTTCTTCAACTCGGTGTGCACGCGCATCCTGCATCGTTCGTACTTCAACAACGATTTCATCTTCGTGCGCCCGGCCA
TCTCGACGGAGTACATCGAGAATGAAGAGTCGCCCACCAAGCCGACGTACCGCGCGTACTACCCCGGCACACGCGATGGC
CTGGGCGCCTGTTTCGAGCGCATCGTCCACAACTTCCAGCTCAACGCGCCGTTTGAAGACCTGGAGCGTGATATCGGCTA
CGTGGTGCGCGCGGTGGGCGAGCACTTCGGCGATTTCCGCATCGCGCCGAATTTCCAGATCCACGTGCTGTCGTCGCTGT
TCTTCCGCAACAAGGCGGCGTACATCATCGGGCGCGTGATCAACGGCGACAAGACGTTCCCGCTGGCCGTGCCGATCCTA
CGCAATGCCGCCGGCCAGCTCTCGCTCGACACGGTGCTGCTGCGGCAGGAACAACTGCTGATCCTGTTCTCGTTCACGCA
CTCGTATTTCATGGTGGACATGGAGATTCCGTCTGCCTACGTGACCTTCCTGCGTGACCTGATGCCGCGCAAGCCGCGCG
CCGAGATCTACACCTCGCTGGGTCTGCAGAAGCAGGGCAAGAACCTCTTCTATCGCGATTTCCTGCATCACCTGCAGCAT
TCGTCGGACAAGTTCATCATCGCGCCGGGCATCAAGGGCCTCGTGATGCTGGTGTTCACGCTGCCGTCGTACCCGTACGT
GTTCAAGGTCATCAAGGACTACTTCCCGCCGCCCAAGGAAACCACGCGCGAGCTGATCAAGAGCAAGTATCAGCTTGTGA
AGCGTCACGACCGCGTGGGACGCATGGCCGATACGCTGGAGTATTCCGACGTGGCCTTCCCGCTCTCGCGCTTTGACGAC
GACCTGATCAAGGAGCTGGAAAAGCACGCGCCTTCGATGGTCGAATACCAGCGCAGCGAAGACGGCGGCGAAGAGATCGT
CATCCGCCACGTCTACATCGAGCGCCGCATGACGCCGCTGAACATCTGGCTGCACGAAGGCACCGATGCGCAGGTCGAGC
ACGGCATCATCGAATACGGCAACGCCATCAAGGAACTCATTGCCGCGAACATCTTCCCGGGCGACATGCTGTACAAGAAC
TTTGGCGTGACGCGCCACGGCCGCGTGGTCTTCTACGACTACGACGAGATCGAATACCTCACCGACTGCAACATCCGCAC
CGTGCCCGAACCGCGCACGGAAGAAGAAGAAATGTCCGGCGAGGTCTGGTACAACGTCGGCCCGCACGACATTTTTCCCG
AGACGTACCGCACGTTCCTGCTGGGCGACCCGCGCGTGCGCGAAGCCTTCCTCAAGCACCACGCAGACTTCTTTGAAGCT
TCCATGTGGCAGGGGTACAAAGAGCGCCTGCTCTCCGGGCAGATGCACGATTTCTACGCGTATGACGCAGCCGAACGCTT
CGTCAATCGCTACGGCGCGAGCGCGCCCGACAACGCAGCACAAGACAACACGACGCTGCAGCGCGCCGCCGCTTAA

Upstream 100 bases:

>100_bases
GCGCAACGGTTTCAGGACGCGAACACGGTACCGAAGACCCTACAATCTAGGTCTGCCTGGTTTGCTGGTTTTGCCGCTTC
CCTGCCCCCGCGCACACGCC

Downstream 100 bases:

>100_bases
CCCTCTCAACACGACGGGAAACCATGCCACAACGCATCAAAGAGCTGTTCGAGAACAACCGGAAGTGGGTCGAGCGGGTC
AACGCTGAAGACCCGGCGTT

Product: bifunctional isocitrate dehydrogenase kinase/phosphatase protein

Products: NA

Alternate protein names: IDH kinase/phosphatase; IDHK/P

Number of amino acids: Translated: 611; Mature: 610

Protein sequence:

>611_residues
MSHFPKLLSSQIAFDVARTMLDGFDKHYRLFREVSVQAKTAFETGDWAGLQQLQRDRIAYYDERVREASVILEDEYDAEN
IDDEIWRQIKLHFIGLLTNHHQPECAETFFNSVCTRILHRSYFNNDFIFVRPAISTEYIENEESPTKPTYRAYYPGTRDG
LGACFERIVHNFQLNAPFEDLERDIGYVVRAVGEHFGDFRIAPNFQIHVLSSLFFRNKAAYIIGRVINGDKTFPLAVPIL
RNAAGQLSLDTVLLRQEQLLILFSFTHSYFMVDMEIPSAYVTFLRDLMPRKPRAEIYTSLGLQKQGKNLFYRDFLHHLQH
SSDKFIIAPGIKGLVMLVFTLPSYPYVFKVIKDYFPPPKETTRELIKSKYQLVKRHDRVGRMADTLEYSDVAFPLSRFDD
DLIKELEKHAPSMVEYQRSEDGGEEIVIRHVYIERRMTPLNIWLHEGTDAQVEHGIIEYGNAIKELIAANIFPGDMLYKN
FGVTRHGRVVFYDYDEIEYLTDCNIRTVPEPRTEEEEMSGEVWYNVGPHDIFPETYRTFLLGDPRVREAFLKHHADFFEA
SMWQGYKERLLSGQMHDFYAYDAAERFVNRYGASAPDNAAQDNTTLQRAAA

Sequences:

>Translated_611_residues
MSHFPKLLSSQIAFDVARTMLDGFDKHYRLFREVSVQAKTAFETGDWAGLQQLQRDRIAYYDERVREASVILEDEYDAEN
IDDEIWRQIKLHFIGLLTNHHQPECAETFFNSVCTRILHRSYFNNDFIFVRPAISTEYIENEESPTKPTYRAYYPGTRDG
LGACFERIVHNFQLNAPFEDLERDIGYVVRAVGEHFGDFRIAPNFQIHVLSSLFFRNKAAYIIGRVINGDKTFPLAVPIL
RNAAGQLSLDTVLLRQEQLLILFSFTHSYFMVDMEIPSAYVTFLRDLMPRKPRAEIYTSLGLQKQGKNLFYRDFLHHLQH
SSDKFIIAPGIKGLVMLVFTLPSYPYVFKVIKDYFPPPKETTRELIKSKYQLVKRHDRVGRMADTLEYSDVAFPLSRFDD
DLIKELEKHAPSMVEYQRSEDGGEEIVIRHVYIERRMTPLNIWLHEGTDAQVEHGIIEYGNAIKELIAANIFPGDMLYKN
FGVTRHGRVVFYDYDEIEYLTDCNIRTVPEPRTEEEEMSGEVWYNVGPHDIFPETYRTFLLGDPRVREAFLKHHADFFEA
SMWQGYKERLLSGQMHDFYAYDAAERFVNRYGASAPDNAAQDNTTLQRAAA
>Mature_610_residues
SHFPKLLSSQIAFDVARTMLDGFDKHYRLFREVSVQAKTAFETGDWAGLQQLQRDRIAYYDERVREASVILEDEYDAENI
DDEIWRQIKLHFIGLLTNHHQPECAETFFNSVCTRILHRSYFNNDFIFVRPAISTEYIENEESPTKPTYRAYYPGTRDGL
GACFERIVHNFQLNAPFEDLERDIGYVVRAVGEHFGDFRIAPNFQIHVLSSLFFRNKAAYIIGRVINGDKTFPLAVPILR
NAAGQLSLDTVLLRQEQLLILFSFTHSYFMVDMEIPSAYVTFLRDLMPRKPRAEIYTSLGLQKQGKNLFYRDFLHHLQHS
SDKFIIAPGIKGLVMLVFTLPSYPYVFKVIKDYFPPPKETTRELIKSKYQLVKRHDRVGRMADTLEYSDVAFPLSRFDDD
LIKELEKHAPSMVEYQRSEDGGEEIVIRHVYIERRMTPLNIWLHEGTDAQVEHGIIEYGNAIKELIAANIFPGDMLYKNF
GVTRHGRVVFYDYDEIEYLTDCNIRTVPEPRTEEEEMSGEVWYNVGPHDIFPETYRTFLLGDPRVREAFLKHHADFFEAS
MWQGYKERLLSGQMHDFYAYDAAERFVNRYGASAPDNAAQDNTTLQRAAA

Specific function: Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source

COG id: COG4579

COG function: function code T; Isocitrate dehydrogenase kinase/phosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AceK family

Homologues:

Organism=Escherichia coli, GI1790446, Length=577, Percent_Identity=41.9410745233969, Blast_Score=461, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACEK_RALPJ (B2UDV1)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001897723.1
- GeneID:   6289796
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0128
- HOGENOM:   HBG298593
- OMA:   RMTPLNI
- ProtClustDB:   PRK02946
- GO:   GO:0005737
- HAMAP:   MF_00747
- InterPro:   IPR010452
- PIRSF:   PIRSF000719
- ProDom:   PD043552

Pfam domain/function: PF06315 AceK

EC number: =2.7.11.5

Molecular weight: Translated: 71186; Mature: 71055

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: ACT_SITE 384-384 BINDING 349-349

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHFPKLLSSQIAFDVARTMLDGFDKHYRLFREVSVQAKTAFETGDWAGLQQLQRDRIAY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
YDERVREASVILEDEYDAENIDDEIWRQIKLHFIGLLTNHHQPECAETFFNSVCTRILHR
HHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
SYFNNDFIFVRPAISTEYIENEESPTKPTYRAYYPGTRDGLGACFERIVHNFQLNAPFED
HHCCCCEEEEECCCCHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHH
LERDIGYVVRAVGEHFGDFRIAPNFQIHVLSSLFFRNKAAYIIGRVINGDKTFPLAVPIL
HHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCHHEEEEEEECCCCCCCCHHHHH
RNAAGQLSLDTVLLRQEQLLILFSFTHSYFMVDMEIPSAYVTFLRDLMPRKPRAEIYTSL
HCCCCCCCHHHHHHHHCCEEEEEEECCCEEEEEEECCHHHHHHHHHHCCCCCHHHHHHHH
GLQKQGKNLFYRDFLHHLQHSSDKFIIAPGIKGLVMLVFTLPSYPYVFKVIKDYFPPPKE
CCHHCCCCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHH
TTRELIKSKYQLVKRHDRVGRMADTLEYSDVAFPLSRFDDDLIKELEKHAPSMVEYQRSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHCCHHHHHHHCC
DGGEEIVIRHVYIERRMTPLNIWLHEGTDAQVEHGIIEYGNAIKELIAANIFPGDMLYKN
CCCCEEEEEEEEHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
FGVTRHGRVVFYDYDEIEYLTDCNIRTVPEPRTEEEEMSGEVWYNVGPHDIFPETYRTFL
CCCCCCCEEEEEECCCCHHEECCCCEECCCCCCCHHHCCCCEEEECCCCCCCHHHHHHHH
LGDPRVREAFLKHHADFFEASMWQGYKERLLSGQMHDFYAYDAAERFVNRYGASAPDNAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
QDNTTLQRAAA
CCCCHHHHHCC
>Mature Secondary Structure 
SHFPKLLSSQIAFDVARTMLDGFDKHYRLFREVSVQAKTAFETGDWAGLQQLQRDRIAY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
YDERVREASVILEDEYDAENIDDEIWRQIKLHFIGLLTNHHQPECAETFFNSVCTRILHR
HHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
SYFNNDFIFVRPAISTEYIENEESPTKPTYRAYYPGTRDGLGACFERIVHNFQLNAPFED
HHCCCCEEEEECCCCHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHH
LERDIGYVVRAVGEHFGDFRIAPNFQIHVLSSLFFRNKAAYIIGRVINGDKTFPLAVPIL
HHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCHHEEEEEEECCCCCCCCHHHHH
RNAAGQLSLDTVLLRQEQLLILFSFTHSYFMVDMEIPSAYVTFLRDLMPRKPRAEIYTSL
HCCCCCCCHHHHHHHHCCEEEEEEECCCEEEEEEECCHHHHHHHHHHCCCCCHHHHHHHH
GLQKQGKNLFYRDFLHHLQHSSDKFIIAPGIKGLVMLVFTLPSYPYVFKVIKDYFPPPKE
CCHHCCCCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHH
TTRELIKSKYQLVKRHDRVGRMADTLEYSDVAFPLSRFDDDLIKELEKHAPSMVEYQRSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHCCHHHHHHHCC
DGGEEIVIRHVYIERRMTPLNIWLHEGTDAQVEHGIIEYGNAIKELIAANIFPGDMLYKN
CCCCEEEEEEEEHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
FGVTRHGRVVFYDYDEIEYLTDCNIRTVPEPRTEEEEMSGEVWYNVGPHDIFPETYRTFL
CCCCCCCEEEEEECCCCHHEECCCCEECCCCCCCHHHCCCCEEEECCCCCCCHHHHHHHH
LGDPRVREAFLKHHADFFEASMWQGYKERLLSGQMHDFYAYDAAERFVNRYGASAPDNAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
QDNTTLQRAAA
CCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA