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The map label for this gene is amtB [C]

Identifier: 187927197

GI number: 187927197

Start: 101262

End: 102464

Strand: Reverse

Name: amtB [C]

Synonym: Rpic_0087

Alternate gene names: 187927197

Gene position: 102464-101262 (Counterclockwise)

Preceding gene: 187927198

Following gene: 187927196

Centisome position: 2.6

GC content: 67.41

Gene sequence:

>1203_bases
ATGAACTCCATCAAAACCGGGGGCGACGCGCTCTTCATCCTGCTGGGCGCGATCATGGTGCTGGCCATGCATGCCGGCTT
CGCGTTCCTTGAGCTGGGCACCGTCCGCAAGAAGAACCAGGTCAACGCACTGGTCAAGATCCTGGTCGACTTTGCCGTGT
CGACCATCGCGTACTTCTTCATCGGCTATTCAGTCGCGTACGGCGTCAACTTCTTTACCGGCGCCGACGTGCTCGCCCAG
CAGAACGGCTACGAGCTGGTGAAGTTCTTCTTCCTGCTGACCTTCGCGGCCGCTATCCCGGCCATCATCTCCGGCGGCAT
TGCCGAGCGCTCGCGCTTCGAGCCGCAACTGGCGGCGACCTTCGTGCTGGTGGGCTTTGTCTACCCGTTCTTTGAGGGCA
TCGCCTGGAACGAGCGCTTCGGTATCCAGCACTGGCTGCAGAGCGTGACGGGCGCGGAGTTCCACGACTTCGCCGGCTCG
GTGGTCGTGCACGCGGTGGGCGGCTGGATCGCGCTGCCGGCGGTGCTGCTGCTGGGCGCCCGCCACGGCCGCTACAAGGA
CGACGGCCGCATCGCCGCGCATCCGCCGTCCAACATCCCGTTCCTGGCCGCCGGCGCCTGGGTGCTGGCGGTGGGCTGGT
TCGGCTTCAACGTGATGAGCGCGCAGACGCTCGACAAGATGAGCGGCCTGGTCGCCATCAACTCGCTGATGGCCATGGTG
GGCGGCACGCTCACCGCGTGGTGGATGGGCAAGAACGATCCGGGCTTCTCGTACAACGGGCCGCTGGCCGGCCTGGTGGC
CGTGTGCGCGGGCTCCGACGTGATGCACCCGCTGGGGGCGCTTGCCGTCGGCGGCATCGCGGGCGTGCTGTTTGTCTGGA
TGTTCACGCGCGTGCAGAACGTCTGGCGCATCGACGACGTGTTGGGCGTGTGGCCGCTGCACGGCCTGTGTGGCGCGTGG
GGTGGCATCGCGGCCGGCATCTTCGGCCTGAAGGCGCTCGGCGGCCTGGGCGGCGTGTCGTTCTGGGCGCAGGTGGCGGG
CACGGCCGGCGGCATTGTCATTGCGCTGGTCGGCGGCACGGTGGTGTACGGCACGCTGCGCAAGCTCGTTGGGCTGCGGT
TGGACCGCGAGGCCGAATTCATGGGCGCCGACCTGGCGATCCACCGCGTCTCGTCCACGCCGGAATCGGAAACGCACTGG
TAA

Upstream 100 bases:

>100_bases
CCGCTGGAACAACTTTTGCATCTGTCGGTGCCTTTTGCGCCATTTCCGGGCACGCACCGCACCCGGATGGCGCTGACCTT
TAACCCGCACGACAACCGCA

Downstream 100 bases:

>100_bases
GCGGAAGCCTGCAGCCAGAGGAAGGCGCGCGCGGCGCTTGCTACAGTGAAAGCGTCTTCCCTCGCCCAACTGCAGGAGTG
CCGCATGCCAGAAATCGATC

Product: Rh family protein/ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MNSIKTGGDALFILLGAIMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFAVSTIAYFFIGYSVAYGVNFFTGADVLAQ
QNGYELVKFFFLLTFAAAIPAIISGGIAERSRFEPQLAATFVLVGFVYPFFEGIAWNERFGIQHWLQSVTGAEFHDFAGS
VVVHAVGGWIALPAVLLLGARHGRYKDDGRIAAHPPSNIPFLAAGAWVLAVGWFGFNVMSAQTLDKMSGLVAINSLMAMV
GGTLTAWWMGKNDPGFSYNGPLAGLVAVCAGSDVMHPLGALAVGGIAGVLFVWMFTRVQNVWRIDDVLGVWPLHGLCGAW
GGIAAGIFGLKALGGLGGVSFWAQVAGTAGGIVIALVGGTVVYGTLRKLVGLRLDREAEFMGADLAIHRVSSTPESETHW

Sequences:

>Translated_400_residues
MNSIKTGGDALFILLGAIMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFAVSTIAYFFIGYSVAYGVNFFTGADVLAQ
QNGYELVKFFFLLTFAAAIPAIISGGIAERSRFEPQLAATFVLVGFVYPFFEGIAWNERFGIQHWLQSVTGAEFHDFAGS
VVVHAVGGWIALPAVLLLGARHGRYKDDGRIAAHPPSNIPFLAAGAWVLAVGWFGFNVMSAQTLDKMSGLVAINSLMAMV
GGTLTAWWMGKNDPGFSYNGPLAGLVAVCAGSDVMHPLGALAVGGIAGVLFVWMFTRVQNVWRIDDVLGVWPLHGLCGAW
GGIAAGIFGLKALGGLGGVSFWAQVAGTAGGIVIALVGGTVVYGTLRKLVGLRLDREAEFMGADLAIHRVSSTPESETHW
>Mature_400_residues
MNSIKTGGDALFILLGAIMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFAVSTIAYFFIGYSVAYGVNFFTGADVLAQ
QNGYELVKFFFLLTFAAAIPAIISGGIAERSRFEPQLAATFVLVGFVYPFFEGIAWNERFGIQHWLQSVTGAEFHDFAGS
VVVHAVGGWIALPAVLLLGARHGRYKDDGRIAAHPPSNIPFLAAGAWVLAVGWFGFNVMSAQTLDKMSGLVAINSLMAMV
GGTLTAWWMGKNDPGFSYNGPLAGLVAVCAGSDVMHPLGALAVGGIAGVLFVWMFTRVQNVWRIDDVLGVWPLHGLCGAW
GGIAAGIFGLKALGGLGGVSFWAQVAGTAGGIVIALVGGTVVYGTLRKLVGLRLDREAEFMGADLAIHRVSSTPESETHW

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=405, Percent_Identity=31.6049382716049, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17550182, Length=428, Percent_Identity=29.2056074766355, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17550180, Length=427, Percent_Identity=28.5714285714286, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI32564042, Length=425, Percent_Identity=27.7647058823529, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI133931100, Length=428, Percent_Identity=26.6355140186916, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321559, Length=416, Percent_Identity=24.7596153846154, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325396, Length=416, Percent_Identity=25.4807692307692, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324187, Length=402, Percent_Identity=25.6218905472637, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI161078314, Length=418, Percent_Identity=28.7081339712919, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 42401; Mature: 42401

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCCCCHHHHCCHHHEEECCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHCCCCCCCHHHHCCHHHEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]