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The map label for this gene is oprM [H]

Identifier: 187927168

GI number: 187927168

Start: 70422

End: 72161

Strand: Direct

Name: oprM [H]

Synonym: Rpic_0058

Alternate gene names: 187927168

Gene position: 70422-72161 (Clockwise)

Preceding gene: 187927167

Following gene: 187927170

Centisome position: 1.79

GC content: 66.32

Gene sequence:

>1740_bases
ATGTGGACGCTGTGGATTCGACGCGCTGACGGTGGTGGTGCATCCCTTGTTTCGTCCCCTGCCGGGGCCGACTTACTTTC
TTTGTCTTGCCAAAGAAAGGTAAGCAAAGAAAGGCGCGCCCGAGATGGCGACTTCCCCTTGAATTTATGTAACCGGGCGG
AGACGGGGAAAACTCGCTCCGCTCAGACAGTTCCCCGTCTCTTTTCCGCCCGCTTACAGAAATTCAAGGCGCCATCTAGG
GCAGGGTACGGCCAAACCGTCGGTGGGCACCTGTTGGCGTTGGGGCGAGCGTTGAGGTGTGGTGAGCGGGCGGCTGGGGC
TGTGTTGATTGCCGCGCTGGCTGTGAGCGGCTGTGCCGTCGGTCCCGATTTCAAAGCGCCCGCGGGGCCTGCGGCTACCG
GCTATTCCGCGCAGCCTGTTTCGCGCGAAACCGCATCCGCGCCCGTTGTTGGCGGCGAAGCGCAGCATCTCGTCGATGCC
AACGTGCCGGCCGATTGGTGGCACCTGTTCCGATCCCCTGCGCTGGATGCGTTGGTGGACGAGGCGTTGCGTGCGAGCCC
GTCCGTCGCGCAGGCGGAGGCGCGCTTGCGTCAGGCGCAGGCCGAAGCGCAGGCGCAGTTCGGCTATGCGTTGCCGTCGG
TGGATGGCACCGTGTCGGCGGTGCGTCAGCAGATCAACCCAGAGGCGTTCGGATTCAACACGCCCAAGCCAGGGCCGTTC
ACGTTGTATTCGGCGTCGCTGTCGGTGTCGTATGCGCTGGACATTTTTGGCGGCGTGCGCCGGGCGCTGGAGGCATCGCG
CGCGCAGGTCGATACGCAGCGTTATGAACTGGAGGCGGCGCGCCTGTCTCTGGCAGGCAATGTGGTGACGACGGCGGTGC
GGATTGCCGCGCTGGATGCGCAGATCGCGACCACGCAGCGATTGGCGGCCGAGCAGCGCAAGCAATTGGAGATCACCGAG
CGGAGGTTGAGCGTGGGCGGCGTCGCGCAGGTCGATGCGTTCTCGCAGCGCACGCTCGTCGCGCAGACCGACGCCACGTT
GCCGCCGCTCGCGCAACAGGCTGCGCAGCAGCGGCACCGTCTATCGGTATTGCTGGGCCGCGAACCCGGTGCCGGGTTGC
CTGACCTGCCGTCGCTCGACGCGCTGCATCTGCCCGACCCGCTGCCGGTGTCGCTGCCATCCACGCTCGCGCAGCGCCGC
CCCGACATCCGTGCTGCCGAAGCGATGTGGCACGAAGCCAGCGCCAACGTGGGCGTGGCTACGGCCAATTTGTTCCCGCG
TATCACCCTGTCCGCCGGGCTGGGTTCGGAAGCCACCAGCTTCCGCAACCTCCTTGGCGCTGGGTCAAGCATCTGGAATC
TGGGCGCCGGCCTCACGCAACCGCTCTTCCACGGCGGCACTCTGCGCGCAAAAAAACGCGAAGCCGAAGCGGCCTACGAC
GCTGCCGGCGCCGCCTACAAACAAGCCGTATTGCAAGGGTTGCAAGAAGTGGCCGACACCCTGCACGCCGTCAACAACGA
CGCCCAAACGCTGCAGGCCCGAGCGCTGGCAACCGACCAGGCACAACAGACCCTGCGCGCCGCCCAAGCCCGCCACGCCG
CCGGCGGCATCAGCACGCTAACGTTGCTGGACGCCCAGCGCCAGGTCGACCAGGCCACACTGCAACAGGTGCAGAGCCAG
GCGGATCGGTTGCTGGATTCCGCCGCGCTCATGCAGGCGTTGGGTGGCGGTTGGCAATAA

Upstream 100 bases:

>100_bases
TTCGAGCTGTGGCCGAGCATCTGGCCGTTGATGGTGTTTACGGTGGTGGTGCTGGGGGTGGCGTTGCGGTTTTATCGCAA
GACGTTGGATTGAGGTGGCT

Downstream 100 bases:

>100_bases
ACCCACGACAAAGGGAAACCCAATCGACAACGCAAGCCTTCAGCTGGTGTGGCCGTACCCTGCCCTAGATGGCGCCTTGA
ATTTCTGTTGCAGGGAGGAA

Product: NodT family RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MWTLWIRRADGGGASLVSSPAGADLLSLSCQRKVSKERRARDGDFPLNLCNRAETGKTRSAQTVPRLFSARLQKFKAPSR
AGYGQTVGGHLLALGRALRCGERAAGAVLIAALAVSGCAVGPDFKAPAGPAATGYSAQPVSRETASAPVVGGEAQHLVDA
NVPADWWHLFRSPALDALVDEALRASPSVAQAEARLRQAQAEAQAQFGYALPSVDGTVSAVRQQINPEAFGFNTPKPGPF
TLYSASLSVSYALDIFGGVRRALEASRAQVDTQRYELEAARLSLAGNVVTTAVRIAALDAQIATTQRLAAEQRKQLEITE
RRLSVGGVAQVDAFSQRTLVAQTDATLPPLAQQAAQQRHRLSVLLGREPGAGLPDLPSLDALHLPDPLPVSLPSTLAQRR
PDIRAAEAMWHEASANVGVATANLFPRITLSAGLGSEATSFRNLLGAGSSIWNLGAGLTQPLFHGGTLRAKKREAEAAYD
AAGAAYKQAVLQGLQEVADTLHAVNNDAQTLQARALATDQAQQTLRAAQARHAAGGISTLTLLDAQRQVDQATLQQVQSQ
ADRLLDSAALMQALGGGWQ

Sequences:

>Translated_579_residues
MWTLWIRRADGGGASLVSSPAGADLLSLSCQRKVSKERRARDGDFPLNLCNRAETGKTRSAQTVPRLFSARLQKFKAPSR
AGYGQTVGGHLLALGRALRCGERAAGAVLIAALAVSGCAVGPDFKAPAGPAATGYSAQPVSRETASAPVVGGEAQHLVDA
NVPADWWHLFRSPALDALVDEALRASPSVAQAEARLRQAQAEAQAQFGYALPSVDGTVSAVRQQINPEAFGFNTPKPGPF
TLYSASLSVSYALDIFGGVRRALEASRAQVDTQRYELEAARLSLAGNVVTTAVRIAALDAQIATTQRLAAEQRKQLEITE
RRLSVGGVAQVDAFSQRTLVAQTDATLPPLAQQAAQQRHRLSVLLGREPGAGLPDLPSLDALHLPDPLPVSLPSTLAQRR
PDIRAAEAMWHEASANVGVATANLFPRITLSAGLGSEATSFRNLLGAGSSIWNLGAGLTQPLFHGGTLRAKKREAEAAYD
AAGAAYKQAVLQGLQEVADTLHAVNNDAQTLQARALATDQAQQTLRAAQARHAAGGISTLTLLDAQRQVDQATLQQVQSQ
ADRLLDSAALMQALGGGWQ
>Mature_579_residues
MWTLWIRRADGGGASLVSSPAGADLLSLSCQRKVSKERRARDGDFPLNLCNRAETGKTRSAQTVPRLFSARLQKFKAPSR
AGYGQTVGGHLLALGRALRCGERAAGAVLIAALAVSGCAVGPDFKAPAGPAATGYSAQPVSRETASAPVVGGEAQHLVDA
NVPADWWHLFRSPALDALVDEALRASPSVAQAEARLRQAQAEAQAQFGYALPSVDGTVSAVRQQINPEAFGFNTPKPGPF
TLYSASLSVSYALDIFGGVRRALEASRAQVDTQRYELEAARLSLAGNVVTTAVRIAALDAQIATTQRLAAEQRKQLEITE
RRLSVGGVAQVDAFSQRTLVAQTDATLPPLAQQAAQQRHRLSVLLGREPGAGLPDLPSLDALHLPDPLPVSLPSTLAQRR
PDIRAAEAMWHEASANVGVATANLFPRITLSAGLGSEATSFRNLLGAGSSIWNLGAGLTQPLFHGGTLRAKKREAEAAYD
AAGAAYKQAVLQGLQEVADTLHAVNNDAQTLQARALATDQAQQTLRAAQARHAAGGISTLTLLDAQRQVDQATLQQVQSQ
ADRLLDSAALMQALGGGWQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=482, Percent_Identity=27.1784232365145, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI2367348, Length=503, Percent_Identity=25.844930417495, Blast_Score=109, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 60932; Mature: 60932

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWTLWIRRADGGGASLVSSPAGADLLSLSCQRKVSKERRARDGDFPLNLCNRAETGKTRS
CEEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCC
AQTVPRLFSARLQKFKAPSRAGYGQTVGGHLLALGRALRCGERAAGAVLIAALAVSGCAV
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
GPDFKAPAGPAATGYSAQPVSRETASAPVVGGEAQHLVDANVPADWWHLFRSPALDALVD
CCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHEECCCCHHHHHHHHCCHHHHHHH
EALRASPSVAQAEARLRQAQAEAQAQFGYALPSVDGTVSAVRQQINPEAFGFNTPKPGPF
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCE
TLYSASLSVSYALDIFGGVRRALEASRAQVDTQRYELEAARLSLAGNVVTTAVRIAALDA
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIATTQRLAAEQRKQLEITERRLSVGGVAQVDAFSQRTLVAQTDATLPPLAQQAAQQRHR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHH
LSVLLGREPGAGLPDLPSLDALHLPDPLPVSLPSTLAQRRPDIRAAEAMWHEASANVGVA
EEHEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCEE
TANLFPRITLSAGLGSEATSFRNLLGAGSSIWNLGAGLTQPLFHGGTLRAKKREAEAAYD
ECCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHH
AAGAAYKQAVLQGLQEVADTLHAVNNDAQTLQARALATDQAQQTLRAAQARHAAGGISTL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
TLLDAQRQVDQATLQQVQSQADRLLDSAALMQALGGGWQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MWTLWIRRADGGGASLVSSPAGADLLSLSCQRKVSKERRARDGDFPLNLCNRAETGKTRS
CEEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCC
AQTVPRLFSARLQKFKAPSRAGYGQTVGGHLLALGRALRCGERAAGAVLIAALAVSGCAV
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
GPDFKAPAGPAATGYSAQPVSRETASAPVVGGEAQHLVDANVPADWWHLFRSPALDALVD
CCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHEECCCCHHHHHHHHCCHHHHHHH
EALRASPSVAQAEARLRQAQAEAQAQFGYALPSVDGTVSAVRQQINPEAFGFNTPKPGPF
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCE
TLYSASLSVSYALDIFGGVRRALEASRAQVDTQRYELEAARLSLAGNVVTTAVRIAALDA
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIATTQRLAAEQRKQLEITERRLSVGGVAQVDAFSQRTLVAQTDATLPPLAQQAAQQRHR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHH
LSVLLGREPGAGLPDLPSLDALHLPDPLPVSLPSTLAQRRPDIRAAEAMWHEASANVGVA
EEHEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCEE
TANLFPRITLSAGLGSEATSFRNLLGAGSSIWNLGAGLTQPLFHGGTLRAKKREAEAAYD
ECCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHH
AAGAAYKQAVLQGLQEVADTLHAVNNDAQTLQARALATDQAQQTLRAAQARHAAGGISTL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
TLLDAQRQVDQATLQQVQSQADRLLDSAALMQALGGGWQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]