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The map label for this gene is ttcA

Identifier: 187927170

GI number: 187927170

Start: 73564

End: 74511

Strand: Direct

Name: ttcA

Synonym: Rpic_0060

Alternate gene names: 187927170

Gene position: 73564-74511 (Clockwise)

Preceding gene: 187927168

Following gene: 187927171

Centisome position: 1.87

GC content: 62.24

Gene sequence:

>948_bases
ATGACGTTTTCCAATAATTTCCACCGCCTCGAAACCCGACTGCAGTCGCAGGTTGGCCGCGCCATCGGCGACTTCAACAT
GATTGAAGACGGCGACACCATCCTCGTGTGCCTGTCCGGCGGCAAGGATTCGTACACGATGCTGTCGGTGCTGATGGCGC
TGCAGAAGCGCGCGCCGGTCGATTTCAAACTGATCGCGATGAACCTCGATCAGAAGCAGCCGGGCTTTCCGGAAGAGGTG
CTGCCCAACTACCTGAAGAAGCTGGGCGTGGAGTATGTGATCGTCGAGGCGGACACGTATTCGATCGTCAAGGAGAAGGT
GCCCGAGGGCAAGACCACGTGCTCGCTGTGCTCGCGCCTGCGCCGCGGCGTGATCTACCGCACGGCCAAGGAGCTGGGCG
CCAACAAGATCGCGCTGGGCCATCACCGCGACGACATCGTCAACACGTTCTTCCTGAACATGTTCTTCGGCGGCAAGATG
AAGTCGATGCCGCCCAAGCTGGCCACCGACAACGGCGACCACATCGTCATCCGGCCGCTCGCCTATTGCGCGGAAAAGGA
CATCGCTTCCTACGCCCGCGCGATGGAATTCCCGATCATTCCGTGCAACCTCTGCGGCTCGCAGGAAAACCTGCAGCGCA
AGAAGGTCAAGGAGATGCTGCTGGAGTGGGAGCGCCAGACGCCGGGCCGCATCGACAACATCTTCGCTTCGCTGCGGAAC
GTCGTGCCGTCGCACCTGGCCGATACCGACCTGTTCGACTTCAACGGCCTCACCACAGGCCTGGCGAAGATCGGCGAAGA
CGCGCTGTTCGGCCAGACTGCCTACGACCAGGCCCCGCTGGTGTTTGCCGGCTCGCACGACGACCGCATCGAGTTCGTGC
GATTTGAGCGCAAGCCGGGCGACAAGTCGGCCGAGCAAGCCGTGCCTGGGGAGGCGGCGGCAAACTGA

Upstream 100 bases:

>100_bases
TGACCCTGCAGACGGCGCCCCGCGCGCGCTAAAATGCTGATTTTCCTCACAATTTGACGCCTGTTCGGCCATTATTCAGC
GCTGGCGGGGTCGAGATGCC

Downstream 100 bases:

>100_bases
GCGGGGCCCGCTCCACACGGGGTGGCACACGGCGCACACATGCGCGCAGCGCGCCATGCCGGGCGTCCACCATCCGTCCG
ACCGGCCAGCGGGCTACCCG

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTFSNNFHRLETRLQSQVGRAIGDFNMIEDGDTILVCLSGGKDSYTMLSVLMALQKRAPVDFKLIAMNLDQKQPGFPEEV
LPNYLKKLGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRLRRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKM
KSMPPKLATDNGDHIVIRPLAYCAEKDIASYARAMEFPIIPCNLCGSQENLQRKKVKEMLLEWERQTPGRIDNIFASLRN
VVPSHLADTDLFDFNGLTTGLAKIGEDALFGQTAYDQAPLVFAGSHDDRIEFVRFERKPGDKSAEQAVPGEAAAN

Sequences:

>Translated_315_residues
MTFSNNFHRLETRLQSQVGRAIGDFNMIEDGDTILVCLSGGKDSYTMLSVLMALQKRAPVDFKLIAMNLDQKQPGFPEEV
LPNYLKKLGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRLRRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKM
KSMPPKLATDNGDHIVIRPLAYCAEKDIASYARAMEFPIIPCNLCGSQENLQRKKVKEMLLEWERQTPGRIDNIFASLRN
VVPSHLADTDLFDFNGLTTGLAKIGEDALFGQTAYDQAPLVFAGSHDDRIEFVRFERKPGDKSAEQAVPGEAAAN
>Mature_314_residues
TFSNNFHRLETRLQSQVGRAIGDFNMIEDGDTILVCLSGGKDSYTMLSVLMALQKRAPVDFKLIAMNLDQKQPGFPEEVL
PNYLKKLGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRLRRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKMK
SMPPKLATDNGDHIVIRPLAYCAEKDIASYARAMEFPIIPCNLCGSQENLQRKKVKEMLLEWERQTPGRIDNIFASLRNV
VPSHLADTDLFDFNGLTTGLAKIGEDALFGQTAYDQAPLVFAGSHDDRIEFVRFERKPGDKSAEQAVPGEAAAN

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=284, Percent_Identity=64.7887323943662, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI193211090, Length=250, Percent_Identity=32, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17540090, Length=245, Percent_Identity=25.7142857142857, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24651972, Length=235, Percent_Identity=25.531914893617, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_RALPJ (B2UD46)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001897657.1
- ProteinModelPortal:   B2UD46
- GeneID:   6288987
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0060
- HOGENOM:   HBG347038
- OMA:   FPHAREE
- ProtClustDB:   PRK10696
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 35144; Mature: 35013

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSNNFHRLETRLQSQVGRAIGDFNMIEDGDTILVCLSGGKDSYTMLSVLMALQKRAPV
CCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEEEECCCCHHHHHHHHHHHHHCCCC
DFKLIAMNLDQKQPGFPEEVLPNYLKKLGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRL
CEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHHH
RRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKMKSMPPKLATDNGDHIVIRPL
HHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEHH
AYCAEKDIASYARAMEFPIIPCNLCGSQENLQRKKVKEMLLEWERQTPGRIDNIFASLRN
HHHHHHHHHHHHHHHCCCCEEHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VVPSHLADTDLFDFNGLTTGLAKIGEDALFGQTAYDQAPLVFAGSHDDRIEFVRFERKPG
HHHHHHCCCCCCCCCCHHHHHHHHCCHHHCCCCCCCCCCEEEECCCCCCEEEEEEECCCC
DKSAEQAVPGEAAAN
CCCHHHCCCCCCCCC
>Mature Secondary Structure 
TFSNNFHRLETRLQSQVGRAIGDFNMIEDGDTILVCLSGGKDSYTMLSVLMALQKRAPV
CCCCHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEEEECCCCHHHHHHHHHHHHHCCCC
DFKLIAMNLDQKQPGFPEEVLPNYLKKLGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRL
CEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHHH
RRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKMKSMPPKLATDNGDHIVIRPL
HHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEHH
AYCAEKDIASYARAMEFPIIPCNLCGSQENLQRKKVKEMLLEWERQTPGRIDNIFASLRN
HHHHHHHHHHHHHHHCCCCEEHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VVPSHLADTDLFDFNGLTTGLAKIGEDALFGQTAYDQAPLVFAGSHDDRIEFVRFERKPG
HHHHHHCCCCCCCCCCHHHHHHHHCCHHHCCCCCCCCCCEEEECCCCCCEEEEEEECCCC
DKSAEQAVPGEAAAN
CCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA