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The map label for this gene is dnaG [H]

Identifier: 187918566

GI number: 187918566

Start: 753658

End: 755439

Strand: Direct

Name: dnaG [H]

Synonym: BH0710

Alternate gene names: 187918566

Gene position: 753658-755439 (Clockwise)

Preceding gene: 187918565

Following gene: 187918567

Centisome position: 81.71

GC content: 30.98

Gene sequence:

>1782_bases
ATGGAATATGCTAAGGTTATAGATTTAATTAAACAAAGAGTAGATATTGTAGGTTTAATAGGCGAGCGTGTTAGATTGGT
TAAAGCAGGAGCGTCTTATAAGGGGCTTTGTCCTTTTCATGCTGAGAAGACACCTTCTTTTTCTATAACCCCTTCTCAGG
GACTTTTTTATTGTTTTGGATGTAGAAGGGGAGGAGATGTCATTAAATTTTTGATGGATATTGAAAAGCTTGATTATAAT
GATGCTGTTAAGTCTTTGTGCAGTAGGATAGGTATTGCATATGACGAGATTAAGAAAACCACGGGATTTAAGAATAAAAC
TCAGGATAAGGCATTAATTTCAAAAATATATGATTTGAACGCTAGATTGGTTAAGACTTTTTTATTTTTTTTAAATAATA
ATCAAGACAATCAAGGGGTTTTGAATTATATTTTGAAAGAGCGGGGGATATCCAAAGAGGTTATTAATTTATTTAATATT
GGTTATTTGCGATGCGATGTTCCAGGTAAGTTAGGTTTTTATGATTTTTTAGTTTTAAAAGGATATTCCGATGAGCTTTT
GAGTAAGAGTGGTTTGTTTGCAAAACAAAATCGAAGATTTTCAATTTTATCTGGAAGATTGATTTTTCCAATCAGAGATT
TTAAAGGAAATGTAGTAGGATTTGGAGGTAGAGATTTAGGTAAAAATAATGGACCTAAATATATTAATTCAAGTGAAACA
GAGGTCTTTAAAAAGAGGGAATTGCTTTATGGGTTTTATGAAGGCTTTTCTGTAATTAGGGAAAGTAAATCTGTGATCTT
AACAGAAGGGTATATAGATGTTCTTTCCTTTTTTACAGCTGGTGTAAAGTTTGCTGTTTCTACACTTGGTACATCTTTTT
CAAGGGAGCATCTTGCTTTAATTAGGAGACATGCAGATAAAATAATGCTGTGCTTTGATGATGATGCTGCTGGGCTTGCA
GCTACCTTTAAGGCTTATCAGATTTGTTTACCGTTTAATGTTGATGTGAGTGTGATTAGGATGAAATACGGGGCTGATCC
TGCAGATGTTTTAAAAAACAAAGGTGCTTCTGTCTTAAAGAATATGATTAATGATAATTGTGAGGCTTTTGAATATCTTT
TGGAAAAATATTCAGCTAAATATGATTTAAATAAAACAACAGATTTAAATGGCATGATTAATTTGTTTATAACTTTGATA
AGTTTGTCAAGTACTAATACGCAGCGAGATCTTCTCTTAGAAAAGCTTGAGAGTAGAGTGGGCATTAAGTTAGAAACCTT
AAGAAAAGATTACTATAGCATGAGAGAGAGGAGTGCAATTGCGAGTTATAAGAAGAATACATCTTCTTATGAGATAAATA
CCTATGAGAGATATTTATTAGTTGCCTTATTGAAAGACTTTAATTATTTTACTATAATAAGGCGTAATATTAGTGATAGT
GACTTGTATGATGTTGATGTGAAAAGAATTTTTGTGTGCTTTGAGAAGCTATTTGAGAATAATAAAGGTTTTTCATTGCT
TAATTTAAGAGAATTATTAAAAAATAAACATAGTGTTAGTGAAGTTTTTTTTGAAAATATGCTACGAGTAGAGTTTGAAG
TGGATGATGAGATGGTTAAGCAAATTTTGTTTGCAATTAAGAAAAGAAAAGTGGAGAATCGAATTTTAGTATTTAAAGAA
ATGAGCAGAGATAATTTTTCAATAGATGCTAAGGCCCAAATAAGAGAATTGATGTTTTTAAATATGCAAAGAGAAAACCT
GAGGATATATTTGAATGAATAG

Upstream 100 bases:

>100_bases
TCCTAAGATTGGTATTCATAAATTTTCTTCAGCTTATAATGACCATCTCTTAGCTGCAAATAGTTATATTCGTAATTTTA
TTACTAAGGATTAAAGAAGT

Downstream 100 bases:

>100_bases
TGTAGAGAATAAAGACTTGCAGGCTTTTAAGAAGAAGAATTCAAAAGCAATAAAGGCTATCTTAAGTTATTTGGGAGACA
AAAAGGTAATTACTTTTGAG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MEYAKVIDLIKQRVDIVGLIGERVRLVKAGASYKGLCPFHAEKTPSFSITPSQGLFYCFGCRRGGDVIKFLMDIEKLDYN
DAVKSLCSRIGIAYDEIKKTTGFKNKTQDKALISKIYDLNARLVKTFLFFLNNNQDNQGVLNYILKERGISKEVINLFNI
GYLRCDVPGKLGFYDFLVLKGYSDELLSKSGLFAKQNRRFSILSGRLIFPIRDFKGNVVGFGGRDLGKNNGPKYINSSET
EVFKKRELLYGFYEGFSVIRESKSVILTEGYIDVLSFFTAGVKFAVSTLGTSFSREHLALIRRHADKIMLCFDDDAAGLA
ATFKAYQICLPFNVDVSVIRMKYGADPADVLKNKGASVLKNMINDNCEAFEYLLEKYSAKYDLNKTTDLNGMINLFITLI
SLSSTNTQRDLLLEKLESRVGIKLETLRKDYYSMRERSAIASYKKNTSSYEINTYERYLLVALLKDFNYFTIIRRNISDS
DLYDVDVKRIFVCFEKLFENNKGFSLLNLRELLKNKHSVSEVFFENMLRVEFEVDDEMVKQILFAIKKRKVENRILVFKE
MSRDNFSIDAKAQIRELMFLNMQRENLRIYLNE

Sequences:

>Translated_593_residues
MEYAKVIDLIKQRVDIVGLIGERVRLVKAGASYKGLCPFHAEKTPSFSITPSQGLFYCFGCRRGGDVIKFLMDIEKLDYN
DAVKSLCSRIGIAYDEIKKTTGFKNKTQDKALISKIYDLNARLVKTFLFFLNNNQDNQGVLNYILKERGISKEVINLFNI
GYLRCDVPGKLGFYDFLVLKGYSDELLSKSGLFAKQNRRFSILSGRLIFPIRDFKGNVVGFGGRDLGKNNGPKYINSSET
EVFKKRELLYGFYEGFSVIRESKSVILTEGYIDVLSFFTAGVKFAVSTLGTSFSREHLALIRRHADKIMLCFDDDAAGLA
ATFKAYQICLPFNVDVSVIRMKYGADPADVLKNKGASVLKNMINDNCEAFEYLLEKYSAKYDLNKTTDLNGMINLFITLI
SLSSTNTQRDLLLEKLESRVGIKLETLRKDYYSMRERSAIASYKKNTSSYEINTYERYLLVALLKDFNYFTIIRRNISDS
DLYDVDVKRIFVCFEKLFENNKGFSLLNLRELLKNKHSVSEVFFENMLRVEFEVDDEMVKQILFAIKKRKVENRILVFKE
MSRDNFSIDAKAQIRELMFLNMQRENLRIYLNE
>Mature_593_residues
MEYAKVIDLIKQRVDIVGLIGERVRLVKAGASYKGLCPFHAEKTPSFSITPSQGLFYCFGCRRGGDVIKFLMDIEKLDYN
DAVKSLCSRIGIAYDEIKKTTGFKNKTQDKALISKIYDLNARLVKTFLFFLNNNQDNQGVLNYILKERGISKEVINLFNI
GYLRCDVPGKLGFYDFLVLKGYSDELLSKSGLFAKQNRRFSILSGRLIFPIRDFKGNVVGFGGRDLGKNNGPKYINSSET
EVFKKRELLYGFYEGFSVIRESKSVILTEGYIDVLSFFTAGVKFAVSTLGTSFSREHLALIRRHADKIMLCFDDDAAGLA
ATFKAYQICLPFNVDVSVIRMKYGADPADVLKNKGASVLKNMINDNCEAFEYLLEKYSAKYDLNKTTDLNGMINLFITLI
SLSSTNTQRDLLLEKLESRVGIKLETLRKDYYSMRERSAIASYKKNTSSYEINTYERYLLVALLKDFNYFTIIRRNISDS
DLYDVDVKRIFVCFEKLFENNKGFSLLNLRELLKNKHSVSEVFFENMLRVEFEVDDEMVKQILFAIKKRKVENRILVFKE
MSRDNFSIDAKAQIRELMFLNMQRENLRIYLNE

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=418, Percent_Identity=32.0574162679426, Blast_Score=213, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 68285; Mature: 68285

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYAKVIDLIKQRVDIVGLIGERVRLVKAGASYKGLCPFHAEKTPSFSITPSQGLFYCFG
CCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCEEECCCCCEEEEEE
CRRGGDVIKFLMDIEKLDYNDAVKSLCSRIGIAYDEIKKTTGFKNKTQDKALISKIYDLN
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHCH
ARLVKTFLFFLNNNQDNQGVLNYILKERGISKEVINLFNIGYLRCDVPGKLGFYDFLVLK
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCCCCCEEEEEEC
GYSDELLSKSGLFAKQNRRFSILSGRLIFPIRDFKGNVVGFGGRDLGKNNGPKYINSSET
CCCHHHHCCCCCCCCCCCCEEEECCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCHH
EVFKKRELLYGFYEGFSVIRESKSVILTEGYIDVLSFFTAGVKFAVSTLGTSFSREHLAL
HHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
IRRHADKIMLCFDDDAAGLAATFKAYQICLPFNVDVSVIRMKYGADPADVLKNKGASVLK
HHHCCCEEEEEECCCCCCHHHHHHHEEEEEECCCCEEEEEEECCCCHHHHHHCCCHHHHH
NMINDNCEAFEYLLEKYSAKYDLNKTTDLNGMINLFITLISLSSTNTQRDLLLEKLESRV
HHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GIKLETLRKDYYSMRERSAIASYKKNTSSYEINTYERYLLVALLKDFNYFTIIRRNISDS
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCEEEEEEECCCCC
DLYDVDVKRIFVCFEKLFENNKGFSLLNLRELLKNKHSVSEVFFENMLRVEFEVDDEMVK
CEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHEEEEECCHHHHH
QILFAIKKRKVENRILVFKEMSRDNFSIDAKAQIRELMFLNMQRENLRIYLNE
HHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure
MEYAKVIDLIKQRVDIVGLIGERVRLVKAGASYKGLCPFHAEKTPSFSITPSQGLFYCFG
CCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCEEECCCCCEEEEEE
CRRGGDVIKFLMDIEKLDYNDAVKSLCSRIGIAYDEIKKTTGFKNKTQDKALISKIYDLN
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHCH
ARLVKTFLFFLNNNQDNQGVLNYILKERGISKEVINLFNIGYLRCDVPGKLGFYDFLVLK
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCCCCCEEEEEEC
GYSDELLSKSGLFAKQNRRFSILSGRLIFPIRDFKGNVVGFGGRDLGKNNGPKYINSSET
CCCHHHHCCCCCCCCCCCCEEEECCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCHH
EVFKKRELLYGFYEGFSVIRESKSVILTEGYIDVLSFFTAGVKFAVSTLGTSFSREHLAL
HHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
IRRHADKIMLCFDDDAAGLAATFKAYQICLPFNVDVSVIRMKYGADPADVLKNKGASVLK
HHHCCCEEEEEECCCCCCHHHHHHHEEEEEECCCCEEEEEEECCCCHHHHHHCCCHHHHH
NMINDNCEAFEYLLEKYSAKYDLNKTTDLNGMINLFITLISLSSTNTQRDLLLEKLESRV
HHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GIKLETLRKDYYSMRERSAIASYKKNTSSYEINTYERYLLVALLKDFNYFTIIRRNISDS
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCEEEEEEECCCCC
DLYDVDVKRIFVCFEKLFENNKGFSLLNLRELLKNKHSVSEVFFENMLRVEFEVDDEMVK
CEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHEEEEECCHHHHH
QILFAIKKRKVENRILVFKEMSRDNFSIDAKAQIRELMFLNMQRENLRIYLNE
HHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]