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The map label for this gene is rpoD [H]

Identifier: 187918567

GI number: 187918567

Start: 755432

End: 757324

Strand: Direct

Name: rpoD [H]

Synonym: BH0712

Alternate gene names: 187918567

Gene position: 755432-757324 (Clockwise)

Preceding gene: 187918566

Following gene: 187918568

Centisome position: 81.91

GC content: 32.01

Gene sequence:

>1893_bases
ATGAATAGTGTAGAGAATAAAGACTTGCAGGCTTTTAAGAAGAAGAATTCAAAAGCAATAAAGGCTATCTTAAGTTATTT
GGGAGACAAAAAGGTAATTACTTTTGAGGACTTATCCACCTTTTTATCAGGGGATATGTTGGAACCTGAAAATATTGATT
ATATGTATGGAGTTCTTGAGAATGAGGGGATAAGTTTAATTAATGAAAAGATGGAGTCAGCTATATGTAGCATTGATGAA
TTTGATGAAGCAAGTAAACTTGATAGTCAGTGTATGATATTAGATGATTCTGTTCAGAGTGATGATGAGCTTGATGATAA
ATTAGATGAATTTGAGGATGAGACTTTAGACAAGGAAGATTTTAGTTCAGGGTATATTAAGAGTGGTTTATTAAAAGATA
GTAATTCTGAAGATCCGATAAGACTTTACTTGAAGGAAATAGGGAAAGAATTCCTATTAACTGGAAATCAAGAGGTTGAG
CTTGCAAAGCAGATGGATTCTGGTGAGAGCATAATTGAGAATATTCTTAAAAATGAAGGACTCGTTATAGAGAATTATTA
TAATTTAGTTAATGCCATTTATTCAAGAGTAGATAAAGAAGAGTTTTTTAAAAAAGAAAAGGAAAGAGAAAAAGATAATA
ATTTTGATTATTATAACAAAAAAAAAAGGATCACTTCCTTTTATAAAGCTTCATTAAAATCGTTTCAGGATCGTTTGGTA
AAATATATTGAAAAGAAGCATAGATTATATGAGCTTGGAGAAGATATTTTTGAAGAGAGTATTAATAATGAGAGACTTCA
TATAAAAGAAATGCTTAAGTCTGTTCCTCTATATCAAGAGGAGTTGCGCATTTTTTCAGATGATTATATTGATTCTGCTA
GCAAAATAAAGGATTTAAAGAGACAACAAAAGTCCATATTGGATAGATTAAAGATAGATAAAGTACGAAATCTTAGAATT
CTTGGGAGAGATTTAGCTATTCCTGAGAGACGGGAGAGGATAGAGCAATCTTTAAATATTCGAGAAGATCTAATTAAAGA
GCAAATTACAGAAGCTCAACTTGCTCAAAAGGAGCTTGAGAGAATTGAGATGTATTATGAATATCCAATGGATAAGATAA
TAAGCATGTCGGAAGAAATCCTTAAGGGCAAGCAGATGATGCAGCATGCAAAAGATCAGTTAATTAAGGCTAATTTAAGA
CTTGTGGTAAGTATTGCCAAAAAGTATGCTAATAGAGGATTGCATTTTTTTGATCTTGTTCAAGAAGGTAATATTGGTCT
AATTAAGGCAGTTGAAAAATTTGAATATAAGCGAGGCTTTAAGTTTTCTACTTATGCTACTTGGTGGATTCGTCAAGCAA
TAACAAGATCAATCTCAGATCAAGCACGTACCATTCGTGTGCCTGTGCATATGATTGAGCAGATAAATAGACTAAATAGA
GAGACAAGATATTTGATTCAAGTGTTAGGTAAAGACCCAACAGATGAGGAGTTATCAGCTAGACTTGGGTGGGATCTTAA
AAAGGTAAAAACTGTAAAAAATGTTTCAAGAGAGCCCGTTTCACTTGAAACACCAATTGGAGAAGAGGAAGATTCTGTTC
TTAGTGATTTTATTGAGGATAAAGCTATAAAAAATCCAGCAAAGCATACATCTTTTGTGGTTTTACAAGATCAAATAAGA
TCAGTTCTTGGCACTCTTCCAGAAAGAGAACAAGAAGTTGTTAAGATGAGATTTGGTCTTGAAGATGGATATTCTTTAAC
CCTTGAAGAGGTTGGACTGCATTTTAATGTTACAAGAGAGAGAATTAGACAGATTGAATCTAAGGCTTTAAGGCGACTTA
AAAATCCTAAGAAAACTCAAAAACTTAAAGATTATTTAGAAGATTTAAATTGA

Upstream 100 bases:

>100_bases
AAAGAAATGAGCAGAGATAATTTTTCAATAGATGCTAAGGCCCAAATAAGAGAATTGATGTTTTTAAATATGCAAAGAGA
AAACCTGAGGATATATTTGA

Downstream 100 bases:

>100_bases
ATAGGAGGCTTCATGGAAAGTAATATCGATATATTGAAGAATCTTGAATGTATATATAAAGCTAAATTTGAGCTTGAGGA
AAGGCAAAAAAATATTCCTA

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MNSVENKDLQAFKKKNSKAIKAILSYLGDKKVITFEDLSTFLSGDMLEPENIDYMYGVLENEGISLINEKMESAICSIDE
FDEASKLDSQCMILDDSVQSDDELDDKLDEFEDETLDKEDFSSGYIKSGLLKDSNSEDPIRLYLKEIGKEFLLTGNQEVE
LAKQMDSGESIIENILKNEGLVIENYYNLVNAIYSRVDKEEFFKKEKEREKDNNFDYYNKKKRITSFYKASLKSFQDRLV
KYIEKKHRLYELGEDIFEESINNERLHIKEMLKSVPLYQEELRIFSDDYIDSASKIKDLKRQQKSILDRLKIDKVRNLRI
LGRDLAIPERRERIEQSLNIREDLIKEQITEAQLAQKELERIEMYYEYPMDKIISMSEEILKGKQMMQHAKDQLIKANLR
LVVSIAKKYANRGLHFFDLVQEGNIGLIKAVEKFEYKRGFKFSTYATWWIRQAITRSISDQARTIRVPVHMIEQINRLNR
ETRYLIQVLGKDPTDEELSARLGWDLKKVKTVKNVSREPVSLETPIGEEEDSVLSDFIEDKAIKNPAKHTSFVVLQDQIR
SVLGTLPEREQEVVKMRFGLEDGYSLTLEEVGLHFNVTRERIRQIESKALRRLKNPKKTQKLKDYLEDLN

Sequences:

>Translated_630_residues
MNSVENKDLQAFKKKNSKAIKAILSYLGDKKVITFEDLSTFLSGDMLEPENIDYMYGVLENEGISLINEKMESAICSIDE
FDEASKLDSQCMILDDSVQSDDELDDKLDEFEDETLDKEDFSSGYIKSGLLKDSNSEDPIRLYLKEIGKEFLLTGNQEVE
LAKQMDSGESIIENILKNEGLVIENYYNLVNAIYSRVDKEEFFKKEKEREKDNNFDYYNKKKRITSFYKASLKSFQDRLV
KYIEKKHRLYELGEDIFEESINNERLHIKEMLKSVPLYQEELRIFSDDYIDSASKIKDLKRQQKSILDRLKIDKVRNLRI
LGRDLAIPERRERIEQSLNIREDLIKEQITEAQLAQKELERIEMYYEYPMDKIISMSEEILKGKQMMQHAKDQLIKANLR
LVVSIAKKYANRGLHFFDLVQEGNIGLIKAVEKFEYKRGFKFSTYATWWIRQAITRSISDQARTIRVPVHMIEQINRLNR
ETRYLIQVLGKDPTDEELSARLGWDLKKVKTVKNVSREPVSLETPIGEEEDSVLSDFIEDKAIKNPAKHTSFVVLQDQIR
SVLGTLPEREQEVVKMRFGLEDGYSLTLEEVGLHFNVTRERIRQIESKALRRLKNPKKTQKLKDYLEDLN
>Mature_630_residues
MNSVENKDLQAFKKKNSKAIKAILSYLGDKKVITFEDLSTFLSGDMLEPENIDYMYGVLENEGISLINEKMESAICSIDE
FDEASKLDSQCMILDDSVQSDDELDDKLDEFEDETLDKEDFSSGYIKSGLLKDSNSEDPIRLYLKEIGKEFLLTGNQEVE
LAKQMDSGESIIENILKNEGLVIENYYNLVNAIYSRVDKEEFFKKEKEREKDNNFDYYNKKKRITSFYKASLKSFQDRLV
KYIEKKHRLYELGEDIFEESINNERLHIKEMLKSVPLYQEELRIFSDDYIDSASKIKDLKRQQKSILDRLKIDKVRNLRI
LGRDLAIPERRERIEQSLNIREDLIKEQITEAQLAQKELERIEMYYEYPMDKIISMSEEILKGKQMMQHAKDQLIKANLR
LVVSIAKKYANRGLHFFDLVQEGNIGLIKAVEKFEYKRGFKFSTYATWWIRQAITRSISDQARTIRVPVHMIEQINRLNR
ETRYLIQVLGKDPTDEELSARLGWDLKKVKTVKNVSREPVSLETPIGEEEDSVLSDFIEDKAIKNPAKHTSFVVLQDQIR
SVLGTLPEREQEVVKMRFGLEDGYSLTLEEVGLHFNVTRERIRQIESKALRRLKNPKKTQKLKDYLEDLN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=278, Percent_Identity=52.158273381295, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI1789098, Length=284, Percent_Identity=37.6760563380282, Blast_Score=199, Evalue=6e-52,
Organism=Escherichia coli, GI1789871, Length=268, Percent_Identity=29.4776119402985, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788231, Length=209, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 73839; Mature: 73839

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSVENKDLQAFKKKNSKAIKAILSYLGDKKVITFEDLSTFLSGDMLEPENIDYMYGVLE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHC
NEGISLINEKMESAICSIDEFDEASKLDSQCMILDDSVQSDDELDDKLDEFEDETLDKED
CCCHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCHHH
FSSGYIKSGLLKDSNSEDPIRLYLKEIGKEFLLTGNQEVELAKQMDSGESIIENILKNEG
CCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHCCC
LVIENYYNLVNAIYSRVDKEEFFKKEKEREKDNNFDYYNKKKRITSFYKASLKSFQDRLV
EEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KYIEKKHRLYELGEDIFEESINNERLHIKEMLKSVPLYQEELRIFSDDYIDSASKIKDLK
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHH
RQQKSILDRLKIDKVRNLRILGRDLAIPERRERIEQSLNIREDLIKEQITEAQLAQKELE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
RIEMYYEYPMDKIISMSEEILKGKQMMQHAKDQLIKANLRLVVSIAKKYANRGLHFFDLV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
QEGNIGLIKAVEKFEYKRGFKFSTYATWWIRQAITRSISDQARTIRVPVHMIEQINRLNR
HCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHH
ETRYLIQVLGKDPTDEELSARLGWDLKKVKTVKNVSREPVSLETPIGEEEDSVLSDFIED
HHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
KAIKNPAKHTSFVVLQDQIRSVLGTLPEREQEVVKMRFGLEDGYSLTLEEVGLHFNVTRE
HHHCCCHHHHHEEEEHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEHHHHCCCHHHHHH
RIRQIESKALRRLKNPKKTQKLKDYLEDLN
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSVENKDLQAFKKKNSKAIKAILSYLGDKKVITFEDLSTFLSGDMLEPENIDYMYGVLE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHC
NEGISLINEKMESAICSIDEFDEASKLDSQCMILDDSVQSDDELDDKLDEFEDETLDKED
CCCHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCHHH
FSSGYIKSGLLKDSNSEDPIRLYLKEIGKEFLLTGNQEVELAKQMDSGESIIENILKNEG
CCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHCCC
LVIENYYNLVNAIYSRVDKEEFFKKEKEREKDNNFDYYNKKKRITSFYKASLKSFQDRLV
EEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KYIEKKHRLYELGEDIFEESINNERLHIKEMLKSVPLYQEELRIFSDDYIDSASKIKDLK
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHH
RQQKSILDRLKIDKVRNLRILGRDLAIPERRERIEQSLNIREDLIKEQITEAQLAQKELE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
RIEMYYEYPMDKIISMSEEILKGKQMMQHAKDQLIKANLRLVVSIAKKYANRGLHFFDLV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
QEGNIGLIKAVEKFEYKRGFKFSTYATWWIRQAITRSISDQARTIRVPVHMIEQINRLNR
HCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHH
ETRYLIQVLGKDPTDEELSARLGWDLKKVKTVKNVSREPVSLETPIGEEEDSVLSDFIED
HHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
KAIKNPAKHTSFVVLQDQIRSVLGTLPEREQEVVKMRFGLEDGYSLTLEEVGLHFNVTRE
HHHCCCHHHHHEEEEHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEHHHHCCCHHHHHH
RIRQIESKALRRLKNPKKTQKLKDYLEDLN
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685; 9084142 [H]