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The map label for this gene is dnaN [H]

Identifier: 187918303

GI number: 187918303

Start: 461913

End: 463073

Strand: Direct

Name: dnaN [H]

Synonym: BH0438

Alternate gene names: 187918303

Gene position: 461913-463073 (Clockwise)

Preceding gene: 187918302

Following gene: 187918304

Centisome position: 50.08

GC content: 30.66

Gene sequence:

>1161_bases
TTGAGTGAGAAATTTATACTTTGCGATACGGAACAAATCTCAGATGAGATTGAAAAAGCCAAAAGCATAATTTTAAACAG
AAACATAAATGATATTTGGAGTGCAATGTTGCTTGAAGTAGAAAATTCCAACCTTACGATAAAAGCAACAGATAGGAATA
TCTTCTTTGAAAGTACAATTCCAATTGTTTCAACAGAAAATTTCAAAGTATTAATTAATGCTTCCAACTTTTCAGATGCT
GTAAAGGCACTAAATTTGTACAATGAACTAAAAATAAAATTTAGAGAAGACGAGAACAGACTAAACATTATTGGAGAATC
AGAAAATAAGAGTGAGAATTGCACAAACGATCATTTAAGTGAACCCACTTTTTCCAATGAAGATATTGAGAGTTATAATT
ATGATATAAATGGAGAGACTTATAATTTTACAGTTGATTTGAACCAAAAAGAGTTTAAGAAGATTATAAACAAGATCTCA
TTCTCAGCATCACATGATGAATCTAAGAATGTTCTAAATGGGATTTATTTCACAAAAAACAAAGATTCTACATTAACACT
TGTCTCTACTAATTCGCATAGAATGTCTGTGTATAAAACCGAATTAGTATTTGAAGAGGATATTAACTTCATAGTGCCTG
TTAAAATGTTTAACCTTTTAAAGCAAATGATGATAGGAGAAGGCATGATAAAGCTCAAGGTTTCTGATAAGAAGTTTTAT
GTTGAGTTCAATAATTATAAAATAGCTTGCAGTCTTATAAATGGGAACTATCCTGATTATGAGAGTATCATACCAAATGA
ACAACAAAACAGATCACTAGTTGAGATTAGTGTTTTAAAAGATAGGCTTGCAAGGGTAAATTCCTACACAGATAAGAGAT
CTAAAAAGGTTATTCTAAATTTCTCCAGTAAACAACTAAGACTTATGGCAGAAGATCCAATTACAGGACGAAAGGGAGAA
TTTTTCATACAAGATCCCAATTATGATTATGAGGGGAGTGAGGAGGCTATAGCTATTAATAGTGCTTACCTTACTGAGGC
AATGGGTGTATTTGATACTTCAAAGTTAGAGATAAAATTCAGTGGAGGAGGTATATTAAAATTGAATGAACCTGATAATT
TTGACTTCATTCATTTAATAATGCCCATGATACTAAACTAA

Upstream 100 bases:

>100_bases
ACTATTAAAATATAATAAAAAAACATACAAATAATCAAAACAAAATTTGCATTCATAAAAGCTAAGAAGAGATGATAATA
TAATACAAGGAGGTAAAACA

Downstream 100 bases:

>100_bases
AATTCTTTCTATTGGCAAGATGATAAAGAAAATTGAGTTTTTTAATTTTAAGAATATAAAAAACCAGGTTATTAATTTTG
ATTTTAATAATATTTATTTT

Product: DNA polymerase III, beta chain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSEKFILCDTEQISDEIEKAKSIILNRNINDIWSAMLLEVENSNLTIKATDRNIFFESTIPIVSTENFKVLINASNFSDA
VKALNLYNELKIKFREDENRLNIIGESENKSENCTNDHLSEPTFSNEDIESYNYDINGETYNFTVDLNQKEFKKIINKIS
FSASHDESKNVLNGIYFTKNKDSTLTLVSTNSHRMSVYKTELVFEEDINFIVPVKMFNLLKQMMIGEGMIKLKVSDKKFY
VEFNNYKIACSLINGNYPDYESIIPNEQQNRSLVEISVLKDRLARVNSYTDKRSKKVILNFSSKQLRLMAEDPITGRKGE
FFIQDPNYDYEGSEEAIAINSAYLTEAMGVFDTSKLEIKFSGGGILKLNEPDNFDFIHLIMPMILN

Sequences:

>Translated_386_residues
MSEKFILCDTEQISDEIEKAKSIILNRNINDIWSAMLLEVENSNLTIKATDRNIFFESTIPIVSTENFKVLINASNFSDA
VKALNLYNELKIKFREDENRLNIIGESENKSENCTNDHLSEPTFSNEDIESYNYDINGETYNFTVDLNQKEFKKIINKIS
FSASHDESKNVLNGIYFTKNKDSTLTLVSTNSHRMSVYKTELVFEEDINFIVPVKMFNLLKQMMIGEGMIKLKVSDKKFY
VEFNNYKIACSLINGNYPDYESIIPNEQQNRSLVEISVLKDRLARVNSYTDKRSKKVILNFSSKQLRLMAEDPITGRKGE
FFIQDPNYDYEGSEEAIAINSAYLTEAMGVFDTSKLEIKFSGGGILKLNEPDNFDFIHLIMPMILN
>Mature_385_residues
SEKFILCDTEQISDEIEKAKSIILNRNINDIWSAMLLEVENSNLTIKATDRNIFFESTIPIVSTENFKVLINASNFSDAV
KALNLYNELKIKFREDENRLNIIGESENKSENCTNDHLSEPTFSNEDIESYNYDINGETYNFTVDLNQKEFKKIINKISF
SASHDESKNVLNGIYFTKNKDSTLTLVSTNSHRMSVYKTELVFEEDINFIVPVKMFNLLKQMMIGEGMIKLKVSDKKFYV
EFNNYKIACSLINGNYPDYESIIPNEQQNRSLVEISVLKDRLARVNSYTDKRSKKVILNFSSKQLRLMAEDPITGRKGEF
FIQDPNYDYEGSEEAIAINSAYLTEAMGVFDTSKLEIKFSGGGILKLNEPDNFDFIHLIMPMILN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=381, Percent_Identity=20.7349081364829, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 44404; Mature: 44273

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKFILCDTEQISDEIEKAKSIILNRNINDIWSAMLLEVENSNLTIKATDRNIFFESTI
CCCCEEEECHHHHHHHHHHHHHEEECCCHHHHHHEEEEEEECCCEEEEECCCEEEEECCC
PIVSTENFKVLINASNFSDAVKALNLYNELKIKFREDENRLNIIGESENKSENCTNDHLS
CEEECCCEEEEEECCCHHHHHHHHHHHEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCC
EPTFSNEDIESYNYDINGETYNFTVDLNQKEFKKIINKISFSASHDESKNVLNGIYFTKN
CCCCCCCCHHHCCEEECCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCHHHEEEEEECC
KDSTLTLVSTNSHRMSVYKTELVFEEDINFIVPVKMFNLLKQMMIGEGMIKLKVSDKKFY
CCCEEEEEECCCCEEEEEEEEEEEECCCCEEEHHHHHHHHHHHHHCCCEEEEEECCCEEE
VEFNNYKIACSLINGNYPDYESIIPNEQQNRSLVEISVLKDRLARVNSYTDKRSKKVILN
EEECCEEEEEEEECCCCCCHHHCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEEEE
FSSKQLRLMAEDPITGRKGEFFIQDPNYDYEGSEEAIAINSAYLTEAMGVFDTSKLEIKF
ECCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEEEEHHHHHHHHCCCCCCEEEEEE
SGGGILKLNEPDNFDFIHLIMPMILN
CCCCEEEECCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SEKFILCDTEQISDEIEKAKSIILNRNINDIWSAMLLEVENSNLTIKATDRNIFFESTI
CCCEEEECHHHHHHHHHHHHHEEECCCHHHHHHEEEEEEECCCEEEEECCCEEEEECCC
PIVSTENFKVLINASNFSDAVKALNLYNELKIKFREDENRLNIIGESENKSENCTNDHLS
CEEECCCEEEEEECCCHHHHHHHHHHHEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCC
EPTFSNEDIESYNYDINGETYNFTVDLNQKEFKKIINKISFSASHDESKNVLNGIYFTKN
CCCCCCCCHHHCCEEECCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCHHHEEEEEECC
KDSTLTLVSTNSHRMSVYKTELVFEEDINFIVPVKMFNLLKQMMIGEGMIKLKVSDKKFY
CCCEEEEEECCCCEEEEEEEEEEEECCCCEEEHHHHHHHHHHHHHCCCEEEEEECCCEEE
VEFNNYKIACSLINGNYPDYESIIPNEQQNRSLVEISVLKDRLARVNSYTDKRSKKVILN
EEECCEEEEEEEECCCCCCHHHCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEEEE
FSSKQLRLMAEDPITGRKGEFFIQDPNYDYEGSEEAIAINSAYLTEAMGVFDTSKLEIKF
ECCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEEEEHHHHHHHHCCCCCCEEEEEE
SGGGILKLNEPDNFDFIHLIMPMILN
CCCCEEEECCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8341609; 9403685; 8359672 [H]